CN212513778U - Sterile sampling device - Google Patents
Sterile sampling device Download PDFInfo
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- CN212513778U CN212513778U CN202020046256.7U CN202020046256U CN212513778U CN 212513778 U CN212513778 U CN 212513778U CN 202020046256 U CN202020046256 U CN 202020046256U CN 212513778 U CN212513778 U CN 212513778U
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Abstract
The invention discloses an aseptic sampling device, which comprises a sampling bottle and a piston, wherein the sampling bottle is connected with the piston; the sampling bottle is provided with two openings, one opening is an exhaust port, an air filter is arranged on the exhaust port, and the other opening is a liquid injection port; the piston comprises a piston cylinder, a limiting pin and a piston column. The piston cylinder, one end is the opening end, inserts the end for the piston post, the other end is the quick interface of health type, is close to the quick interface of health type and is equipped with an opening in piston cylinder length direction, its opening is unanimous with the liquid filling opening size of sampling bottle, the position corresponds from top to bottom, sampling bottle and piston can integrated into one piece, also can process the back bonding together alone. The sterile sampling device has the advantages of convenience in carrying and operation, capability of remarkably reducing sampling cost and the like.
Description
Technical Field
The invention relates to an aseptic sampling device, in particular to a disposable aseptic sampling device, and belongs to the technical field of biology.
Background
At present, in the biological culture process, a timing sampling is needed to analyze whether the biological culture process is polluted, so that the sampling process needs to prevent the sampled sample from being polluted by the environment, and an online sampling device is particularly needed. However, the existing devices have complex structure, inconvenient carrying and complex operation.
Like the CN209759434U utility model that has authorized discloses an aseptic sample cup, when the sample, need sterilize to whole sample cup with steam, this just has high temperature and high pressure resistant requirement to the material of sample cup, when sampling the cup sterilization simultaneously, still has the risk that takes place to burst, therefore the device is with high costs, and has the potential safety hazard. But when in use, the reactor is connected with the sampling end of the reactor and is sterilized on line at the same time, so that the one-time sterilization time can be saved compared with other products of the same type.
CN201821474223.1 utility model the device include stock solution portion, sample portion, first steam pipe and second steam pipe, whole structure includes 2 valves and 2 sections pipelines at least, the operation is more complicated, compares with CN209759434U patent, this set of device is safer, but in use needs twice sterilization, it is not convenient to operate.
The CN203148726U patent is similar to 201821474223 and has similar disadvantages because the device has a complicated structure, which is difficult to be completed by one person during operation, and the valve pipe and the sampling bottle are carefully cleaned after use, so the operation convenience is greatly restricted.
To sum up, present sampling device has the valve pipeline many, carries inconvenient, complex operation and is difficult to after the use technical problem such as clean.
Disclosure of Invention
The invention aims to solve the technical problems that the sampling device has more valve pipelines, is complex in design, is not easy to operate, is not easy to clean after being used and the like, and provides the sterile sampling device; and through processing sampling bottle and piston part into disposable consumptive material, the bagging-off is adorned after disinfecting in advance, takes out when using and connects on the tee bend through the clamp, therefore the operation is simple and direct.
Technical scheme of the invention
An aseptic sampling device comprises a sampling bottle and a piston;
the sampling bottle is provided with two openings, one opening is an exhaust opening, an air filter is arranged on the exhaust opening, the other opening is a liquid injection opening, and the volume of the sampling bottle is 5mL-5000 mL;
the piston is arranged on the sampling bottle;
the piston comprises a piston cylinder, a limiting pin and a piston column, and the piston column is arranged in the piston cylinder;
the upper wall of the sampling bottle is used as the lower wall of the piston cylinder, and the piston cylinder and the sampling bottle are integrally processed and molded; or the piston cylinder and the sampling bottle are respectively processed, and a liquid injection port of the sampling bottle is vertically aligned with a liquid outflow port on the piston cylinder and then is adhered into a whole by using an adhesive;
the sampling bottle and the piston are made of glass, metal, silica gel, resin, waterproof paper boards, plastics or rubber and the like;
one end of the piston cylinder is an open end and is a piston column insertion end, the other end of the piston cylinder is a first quick connector, and the first quick connector is a flange or a chuck with an opening in the middle;
when the sampling device is used, the limiting pin is pulled out from the second limiting hole and placed in the first limiting hole, so that the piston column cannot be pulled out of the piston cylinder to pollute the sampling bottle;
the piston column consists of a pull rod and a plunger, the pull rod is used for pushing and pulling the piston column, 1-2 annular O-shaped ring grooves are respectively formed in one end, close to the pull rod, of the plunger and one end, close to the first quick connector, of the plunger, and the O-shaped rings are rotated in the grooves, so that the sealing performance of the device is further improved;
a liquid outlet is arranged below the piston cylinder in the length direction and close to the first quick connector, and the liquid outlet is in vertical positive correspondence with the liquid inlet of the sampling bottle, and has the same shape and the same opening size; the size of the liquid outlet on the piston cylinder is 2mm-100mm, and the liquid outlet can be round or square;
can add 1 ~ 4 exports on the foretell sample bottle for with other aseptic bottles butt joints to expand the sample volume of this device, each export of addding adopts the spiral cover to seal.
The aseptic sampling device is put into an aseptic bag after being sterilized, or is put into a packaging bag and then sterilized, and is stored separately, and only when the aseptic sampling device is used, the packaging bag is disassembled again, and the first quick connector of the piston cylinder is connected with a tee joint through a hoop.
The sterile sampling device is used for sampling fermentation liquor in the biological fermentation process, sampling in the cell culture process and sampling water for injection, and also can be used for sampling liquid food, cosmetics, blood products or clean gas.
The method for using the sterile sampling device is specifically shown in fig. 4:
firstly, taking a tee joint, and then connecting a first tee joint in the tee joint with a sampling tube of a device to be sampled;
then connecting a second way of the three ways with a discharge valve;
then, a third tee in the tee is connected with the first quick connector of the piston cylinder through a hoop;
opening a steam valve 5 and a valve 7 on a sampling pipe of the device to be sampled, slowly opening a discharge valve 8 at the lower end of the tee joint to allow a small amount of steam to be emitted so as to keep a certain steam pressure in the sampling pipe, allowing the steam to enter the tee joint from top to bottom along the pipe and diffuse to the outer side of a first quick connector of a piston cylinder of the sterile sampling device, sterilizing the outer side of the first quick connector at high temperature by the steam at the position, closing the steam valve 5 after 10 minutes, opening a liquid discharge valve 6 of the device to be sampled, allowing the liquid to flow out of the discharge valve 8 along the pipe, and closing the discharge valve 8 after the liquid flows out;
then the piston limit pin is pulled out from the second limit hole and placed in the first limit hole, the piston column is pulled outwards until the piston column is clamped by the limit pin of the first limit hole, at the moment, the sample liquid to be sampled begins to enter the sampling bottle, when the volume meets the requirement, the piston column is pushed back to the end of the piston cylinder, the piston limit pin is pulled out from the first limit hole and placed in the second limit hole, the liquid discharge valve 6 of the device to be sampled is closed, the discharge valve 8 is opened, the steam valve 5 is opened, at the moment, the liquid in the pipeline can be removed by the steam, and the steam valve 5 and the valve 7 are closed;
and after sampling is finished, opening the clamp connected with the tee joint and the piston cylinder, and taking down the sterile sampling device. Then, in a sterile room, the air filter on the vent hole of the sampling bottle is pulled out, and the sample liquid is taken out by an instrument from the vent hole.
The invention has the beneficial technical effects
According to the sterile sampling device, the sampling bottle and the piston part of the sterile sampling device are integrally designed, so that a plurality of valves are omitted, the design scheme is simple, and the device is convenient to carry.
Furthermore, the sampling bottle and the piston part are processed into disposable consumables, the consumables are sterilized in advance and then bagged, and the consumables are taken out and directly connected to the tee joint when the aseptic sampling device is used, so that one-time sterilization operation is reduced, cleaning is avoided, operation time is saved, and operation cost is reduced. Therefore, the invention has the advantages of convenient carrying, convenient operation, obvious reduction of sampling cost and the like.
Drawings
FIG. 1 is a schematic view showing the general structure of the sterile sampling device of example 1;
FIG. 2, cross-sectional view of a sampling vial;
FIG. 3, a cross-sectional view of the piston;
FIG. 4 is a schematic view showing the connection of the sterile sampling device to the bioreactor;
fig. 5 is a schematic view of the general structure of the sterile sampling device of example 2.
Fig. 6 is a schematic view of the general structure of the sterile sampling device of embodiment 3.
Detailed description of the preferred embodiments
The invention is further illustrated, but not limited, by the following specific examples in connection with the accompanying drawings.
Detailed description of the preferred embodiment 1
An aseptic sampling device, as shown in figure 1, comprises a sampling bottle 1 and a piston 2, wherein the volume of the sampling bottle is 10 mL;
as shown in fig. 2, the sampling bottle 1 has two openings on the upper portion of the bottle wall of the sampling bottle 1, one is an exhaust port 11, the exhaust port 11 is used for installing the air filter 4, and the other is a liquid injection port 12;
the piston 2 is arranged on the sampling bottle 1;
the piston 2 is specifically shown in fig. 3, and includes a piston cylinder 21, a limiting pin 22 and a piston column 23, the piston column 23 is installed in the piston cylinder 21, two limiting holes, namely a first limiting hole 24 and a second limiting hole 25, are arranged above the piston cylinder 21, and the position of the piston column 23 in the piston cylinder 21 is controlled by inserting and pulling the limiting pin 22 in the first limiting hole 24 and the second limiting hole 25;
the end of the piston column 23 is provided with a pull rod 232 for pushing and pulling the plunger 233, two ends of the plunger 233 are respectively provided with an annular groove 231, and the plunger 233 and the pull rod 232 are integrally formed;
the total length of the wall of the piston cylinder 21 is 1/2-2/3 of the length of the upper wall of the sampling bottle, and the width of the wall of the piston cylinder 21 is consistent with that of the sampling bottle 1;
the upper wall of the sampling bottle 1 is used as the lower wall of the piston cylinder 21, namely the piston cylinder 21 and the sampling bottle 1 are integrally processed and molded;
one end 211 of the piston cylinder 21 is an open end and is an insertion end of the piston column 23, the other end 214 is a first quick connector, the first quick connector is a sanitary chuck with a middle opening, and the middle opening 212 is a plunger inlet;
a liquid outlet 213 with a 5mm long rectangular opening is arranged at the position of the piston cylinder 21 close to the sanitary chuck along the length direction, and the liquid outlet 213 is consistent with the liquid injection port 12 of the sampling bottle 1 in shape and size and corresponds to the liquid injection port up and down in position;
in this embodiment, the plunger cylinder 21 of the plunger is integrally formed with the sampling bottle 1, that is, the upper wall of the sampling bottle 1 is used as the lower wall of the plunger cylinder 21, specifically, as shown in the upper wall of the sampling bottle 1 in fig. 2, the liquid injection port 12 of the sampling bottle 1 is a liquid outflow port 213 on the plunger cylinder 21, and the opening size of the liquid outflow port is a rectangular port 5mm long;
the sampling bottle 1 and the piston 2 are made of polypropylene.
The method for using the sampling device to sample in the microbial fermentation process (refer to fig. 5) is as follows:
firstly, preparing a tee joint, connecting a first tee joint in the tee joint with a sampling pipe of a device to be sampled, and connecting a second tee joint in the tee joint with a discharge valve; then, the third joint is connected with the first quick joint of the piston cylinder through a hoop;
then opening a steam valve 5 and a valve 7 on a sampling pipe of the device to be sampled, slowly opening a discharge valve 8 to allow a small amount of steam to be emitted so as to keep a certain steam pressure in the sampling pipe, at the moment, the steam enters a tee joint from top to bottom along the pipe and is diffused to the outer side of a first quick connector of a piston cylinder of the sterile sampling device, performing high-temperature sterilization on the outer side of the first quick connector by the steam, closing the steam valve 5 after 10 minutes, opening a liquid discharge valve 6 of the device to be sampled, allowing liquid to flow out of the discharge valve 8 along the pipe, and closing the discharge valve 8 after liquid flows out;
then the piston limit pin is pulled out from the second limit hole and placed in the first limit hole, the piston column is pulled outwards until the piston column is clamped by the limit pin of the first limit hole, at the moment, the sample liquid to be sampled begins to enter the sampling bottle, when the volume meets the requirement, the piston column is pushed back to the end of the piston cylinder, the piston limit pin is pulled out from the first limit hole and placed in the second limit hole, at the moment, the liquid discharge valve 6 of the device to be sampled is closed, the discharge valve 8 is opened, the steam valve 5 is opened, at the moment, the liquid in the pipeline can be removed by the steam, and the steam valve 5 and the valve 7 are closed;
and (4) after sampling, the opened tee joint is connected with the hoop of the piston cylinder, and the sterile sampling device is taken down. Then, in a sterile room, the air filter on the vent hole of the sampling bottle is pulled out, and the sample liquid is taken out by an instrument from the vent hole.
Detailed description of the preferred embodiment 2
A sterile sampling device is prepared by processing a piston cylinder and a sampling bottle respectively, aligning a liquid injection port of the sampling bottle with a liquid outflow port on the piston cylinder up and down, and adhering the sampling bottle and the liquid outflow port to form a whole body by using an adhesive, wherein the rest is the same as example 1, and is specifically shown in FIG. 5.
Detailed description of preferred embodiments 3
An aseptic sampling device, the upper wall of the said sampling bottle 1 is regarded as the lower wall of the piston cylinder 21, namely the piston cylinder 21 is integrally processed and shaped with the sampling bottle 1; an "O" ring is placed in the plunger groove 231 and can be tightly attached to the inner wall of the piston cylinder to enhance the sterility of the device, which is otherwise the same as in embodiment 1, as shown in fig. 6.
In conclusion, compared with the existing device, the sampling device capable of sterilizing on line provided by the invention reduces a plurality of valves, has the advantages of convenience in carrying and operation, capability of obviously reducing sampling cost and the like, and is very suitable for on-line sterile sampling operation in microbial fermentation and cell culture processes.
The foregoing is merely an example of the embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (8)
1. An aseptic sampling device comprises a sampling bottle and is characterized by also comprising a piston;
the sampling bottle is provided with two openings, one opening is an exhaust port, an air filter is arranged on the exhaust port, and the other opening is a liquid injection port;
the piston is arranged on the sampling bottle;
the piston comprises a piston cylinder, a limiting pin and a piston column, and the piston column is arranged in the piston cylinder;
one end of the piston cylinder is an open end and is a piston column insertion end, the other end of the piston cylinder is a first quick connector, and the first quick connector is a flange or a chuck with an opening in the middle;
two limiting holes, namely a first limiting hole and a second limiting hole, are arranged above the piston cylinder, and the position of the piston column in the piston cylinder can be controlled by switching the limiting pin in the two limiting holes;
a liquid outlet is arranged below the piston cylinder in the length direction and close to the first quick connector, and the liquid outlet is in vertical positive correspondence with the liquid inlet of the sampling bottle and has the same shape;
the piston column consists of a pull rod and a plunger, and the pull rod is used for pushing and pulling the piston column.
2. An aseptic sampling device as defined in claim 1, wherein the upper wall of the sampling vial is formed as the lower wall of a plunger barrel which is integrally formed with the sampling vial.
3. An aseptic sampling device as defined in claim 1, wherein said piston cylinder is formed separately from the sampling bottle, and the liquid inlet of the sampling bottle is aligned with the liquid outlet of the piston cylinder and bonded to the liquid inlet of the sampling bottle by an adhesive or welded by a welding machine.
4. An aseptic sampling device as defined in claim 1 wherein the liquid discharge opening in the barrel is sized to be between 2mm and 100 mm.
5. An aseptic sampling device as defined in claim 1 in which the sampling bottle has a volume of 5mL to 5000 mL.
6. An aseptic sampling device as defined in claim 1, wherein the plunger has 1-2 annular recesses at its upper end near the pull rod and at its end near the first quick connector, the planes of the recesses being perpendicular to the axis of the plunger.
7. An aseptic sampling device as defined in claim 1, wherein said sampling bottle and said piston are made of glass, metal, silica gel, resin, waterproof cardboard, plastic or rubber.
8. An aseptic sampling device as defined in claim 1, wherein 1-4 outlets are additionally provided on said sampling bottle and sealed by screw cap.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020046256.7U CN212513778U (en) | 2020-01-09 | 2020-01-09 | Sterile sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020046256.7U CN212513778U (en) | 2020-01-09 | 2020-01-09 | Sterile sampling device |
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| Publication Number | Publication Date |
|---|---|
| CN212513778U true CN212513778U (en) | 2021-02-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202020046256.7U Active CN212513778U (en) | 2020-01-09 | 2020-01-09 | Sterile sampling device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113481082A (en) * | 2021-07-13 | 2021-10-08 | 深圳赛桥生物创新技术有限公司 | Sterile cell sampling device and method based on injector |
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2020
- 2020-01-09 CN CN202020046256.7U patent/CN212513778U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113481082A (en) * | 2021-07-13 | 2021-10-08 | 深圳赛桥生物创新技术有限公司 | Sterile cell sampling device and method based on injector |
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