CN218989234U

CN218989234U - Device for transferring culture medium

Info

Publication number: CN218989234U
Application number: CN202320137418.1U
Authority: CN
Inventors: 魏东兵
Original assignee: Zhongbo Ruikang Shanghai Biotechnology Co ltd
Current assignee: Zhongbo Ruikang Shanghai Biotechnology Co ltd
Priority date: 2023-02-07
Filing date: 2023-02-07
Publication date: 2023-05-09
Anticipated expiration: 2033-02-07

Abstract

The utility model discloses a device for transferring a culture medium, which comprises a cover body, a liquid conduit, a gas conduit and a transfer bag, wherein the bottom of the cover body is hollowed to form a cavity structure for accommodating a culture bottle mouth, an internal thread matched with the thread of the culture bottle mouth is arranged on the inner side of the cover body, a first connecting pipe and a second connecting pipe are integrally connected to the top of the cover body, the first connecting pipe is connected with the liquid conduit, the second connecting pipe is connected with the gas conduit, a joint area is sealed by glue injection, an air filter is arranged at the tail end of the gas conduit and used for filtering cell culture tail gas, a first flow stopping clamp is arranged on the liquid conduit, a second flow stopping clamp is arranged on the gas conduit, and the liquid conduit is connected to the transfer bag. The device can realize the quick aseptic transfer of culture medium in the enclosed environment, easy operation prevents pollution, very big improvement the transfer efficiency of culture medium, and device structure is succinct, and the reliability is high, and the cost is lower.

Description

Device for transferring culture medium

Technical Field

The utility model belongs to the technical field of biological equipment, and particularly relates to a device for transferring a culture medium.

Background

The process requirements of biological cell culture technology are strict, and the working environment and conditions must be ensured to be free of microorganisms and not affected by other harmful factors. In order to prevent microbial contamination and the influence of harmful factors, the cell culture room is required to be clean in environment, fresh in air, dry and dust-free. When bottled culture medium need pour into from the bottle and shift the bag in, perhaps pour out the culture solution and accomplish the biological cell sample and produce the pollution risk easily, open the bottle lid and empty the in-process of pouring, introduce exogenous impurity into the culture solution easily in the middle of, in addition, current device increases along with the use number of times, can undoubtedly increase the possibility of retaining the bacterium in the bottle. The pollution prevention measure of the cell culture technology is a key for determining success or failure of cell culture, and the whole transfer process has very high requirement on sterility, cannot generate any pollution and is convenient and quick. At present, bottled culture medium can only be manually poured into a transfer bag from a bottle, the operation process is complex, pollution is easy to occur, and the culture medium is wasted.

Disclosure of Invention

In order to overcome the problems in the related art, the present utility model provides a device for transferring a culture medium, which can rapidly transfer the culture medium from a storage bottle to a transfer bag, improve production efficiency, and prevent contamination.

In order to at least achieve one of the above purposes, the present utility model adopts the following technical scheme:

the utility model provides a device for culture medium shifts, includes lid, liquid conduit, gas conduit, lid bottom fretwork forms the cavity structure who holds the cultivation bottleneck, just the lid inboard be equipped with cultivate the internal thread of bottleneck screw thread looks adaptation, lid top integration is connected with first takeover and second takeover, first takeover is connected with liquid conduit, the second takeover is connected with gas conduit, gas conduit's end is equipped with air cleaner.

Further, the first connecting pipe is inserted into the liquid conduit, and the joint area of the liquid conduit and the first connecting pipe is sealed by glue injection, and the first connecting pipe and the pipe cavity of the liquid conduit are communicated with the cavity structure of the cover body.

Further, the second connecting pipe is inserted into the gas conduit, and the joint area of the gas conduit and the second connecting pipe is sealed by glue injection, and the second connecting pipe and the pipe cavity of the gas conduit are communicated with the cavity structure of the cover body.

Further, the outer edge of the cover body is provided with anti-skid patterns, so that a user can conveniently assemble and disassemble the cover body.

Further, a first flow stop clip is arranged on the liquid conduit.

Further, a second flow stopping clamp is arranged on the gas conduit.

Further, the first adapter tube is provided with an annular protrusion protruding radially from an outer surface thereof, so that the liquid conduit and the first adapter tube are interference fit.

Further, the second adapter tube is provided with an annular protrusion protruding radially from an outer surface thereof, so that the gas conduit and the second adapter tube are interference fit.

Further, the air filter is a disc filter for treating cell culture tail gas.

Further, the device for transferring culture medium further comprises a transfer bag, and the liquid conduit is connected to the transfer bag.

Further, the cover body is provided with a number.

Further, the cover body, the second connecting pipe and the second connecting pipe are integrally formed, so that the production and the manufacturing are facilitated.

Compared with the prior art, the utility model has the following beneficial effects:

the device can realize the quick aseptic transfer of culture medium in the enclosed environment, easy operation prevents pollution, very big improvement the transfer efficiency of culture medium, and device structure is succinct, and the reliability is high, and the cost is lower.

Drawings

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is a schematic view of the overall structure of the present utility model connected to a transfer bag;

FIG. 3 is a front view of the cover of the present utility model;

FIG. 4 is a cross-sectional view taken along the direction A-A in FIG. 3 in accordance with the present utility model;

the components in the figures are labeled as follows: 1-lid, 2-internal screw thread, 3-first takeover, 4-second takeover, 5-liquid conduit, 6-gas conduit, 7-air cleaner, 8-first stop clamp, 9-second stop clamp, 10-transfer bag.

Detailed Description

The advantages and features of the present utility model will become more apparent from the following description when taken in conjunction with the accompanying drawings. It should be understood that the embodiments are illustrative only and should not be taken as limiting the scope of the utility model.

It should be noted that the terms "first" and "second" in the claims and the specification of this application are used only for describing similar objects, and are not necessarily to be construed as a specific order or sequence.

In the present utility model, unless explicitly specified and limited otherwise, the terms "fixedly attached," "connected," and the like are to be construed broadly and, for example, as a fixed connection, as a removable connection, or as a unit; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

Fig. 1 to 2 show the overall structure of the present utility model.

The utility model comprises a cover body 1, a liquid conduit 5 and a gas conduit 6. The top of the cover body 1 is integrally connected with a first connecting pipe 3 and a second connecting pipe 4, the first connecting pipe 3 is connected with a liquid conduit 5, the second connecting pipe 4 is connected with a gas conduit 6, and the tail end of the gas conduit 6 is provided with an air filter 7 for treating cell culture tail gas. The utility model also comprises a transfer bag 10, to which transfer bag 10 the liquid conduit 5 is connected.

The liquid conduit 5 is provided with a first flow stopping clamp 8; the gas conduit 6 is provided with a second flow stop clip 9.

The first connecting pipe 3 is inserted into the liquid conduit 5, the joint area of the liquid conduit 5 and the first connecting pipe 3 is sealed by glue injection, and the first connecting pipe 3 and the pipe cavity of the liquid conduit 5 are communicated with the cavity structure of the cover body 1; the second connection tube 4 is inserted into the gas conduit 6, and the joint area of the gas conduit 6 and the second connection tube 4 is sealed by glue injection, and the second connection tube 4 and the tube cavity of the gas conduit 6 are communicated with the cavity structure of the cover body 1.

The first adapter tube 3 is provided with an annular protrusion protruding radially from the outer surface thereof, so that the liquid conduit 5 and the first adapter tube 3 are in interference fit; the second adapter tube 4 is provided with an annular protrusion protruding radially from its outer surface, such that the gas conduit 6 and the second adapter tube 4 are interference fit.

In the present embodiment, the air filter 7 is a disc filter, and the cover 1 is provided with a number.

Fig. 3 to 4 show a specific structure of the cover body in the present utility model.

The bottom of the cover body 1 is hollowed out to form a cavity structure for accommodating the culture bottle mouth, the inner side of the cover body 1 is provided with an internal thread 2 matched with the thread of the culture bottle mouth, the outer edge of the cover body 1 is provided with anti-slip threads, and a user can conveniently assemble and disassemble the cover body 1. The cover body 1, the first connecting pipe 3 and the second connecting pipe 4 are integrally formed, so that the production and the manufacturing are facilitated.

In order to better understand the technical solution of the present utility model, the following description will be given by taking the pouring of the bottled medium from the bottle into the transfer bag as an example, the use method of the present utility model:

1) Under the A-level clean environment, the bottle cap of the culture medium bottle is unscrewed, replaced by the cover body of the culture medium bottle, and fastened to ensure the tightness.

2) The first connecting pipe and the second connecting pipe are respectively inserted into the liquid conduit and the gas conduit, then the joint area is sealed by glue injection, and the other end of the first connecting pipe is connected with the transfer bag, so that the tightness is ensured.

3) And opening the first flow stopping clamp, closing the second flow stopping clamp, allowing the culture medium to flow from the bottle to the transfer bag, and transferring the transfer bag from the A-level clean environment to the C-level environment to complete aseptic transfer of the culture medium in the whole transfer process.

The above description is only of the preferred embodiments of the present utility model and is not intended to limit the present utility model, and various modifications and variations may be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims

1. An apparatus for media transfer, characterized by: comprises a cover body (1), a liquid conduit (5) and a gas conduit (6); the bottom of the cover body (1) is hollowed out to form a cavity structure for accommodating a culture bottle mouth, an internal thread (2) matched with the thread of the culture bottle mouth is arranged on the inner side of the cover body (1), a first connecting pipe (3) and a second connecting pipe (4) are integrally connected to the top of the cover body (1), the first connecting pipe (3) is connected with a liquid conduit (5), the second connecting pipe (4) is connected with a gas conduit (6), and the tail end of the gas conduit (6) is provided with an air filter (7).

2. The apparatus for media transfer of claim 1, wherein: the first connecting pipe (3) is inserted into the liquid conduit (5), the joint area of the liquid conduit (5) and the first connecting pipe (3) is sealed by glue injection, and the first connecting pipe (3) and the pipe cavity of the liquid conduit (5) are communicated with the cavity structure of the cover body (1).

3. The apparatus for media transfer of claim 1, wherein: the second connecting pipe (4) is inserted into the gas conduit (6), and the joint area of the gas conduit (6) and the second connecting pipe (4) is sealed by glue injection, and the second connecting pipe (4) and the pipe cavity of the gas conduit (6) are communicated with the cavity structure of the cover body (1).

4. The apparatus for media transfer of claim 1, wherein: the outer edge of the cover body (1) is provided with anti-skid patterns.

5. The apparatus for media transfer of claim 1, wherein: the liquid conduit (5) is provided with a first flow stopping clamp (8).

6. The apparatus for media transfer of claim 1, wherein: the gas conduit (6) is provided with a second flow stopping clamp (9).

7. The apparatus for media transfer of claim 1, wherein: the first adapter tube (3) is provided with an annular protrusion protruding radially from the outer surface thereof, so that the liquid conduit (5) and the first adapter tube (3) are in interference fit.

8. The apparatus for media transfer of claim 1, wherein: the second connection tube (4) is provided with an annular protrusion protruding radially from the outer surface thereof, so that the gas conduit (6) and the second connection tube (4) are in interference fit.

9. The apparatus for media transfer of claim 1, wherein: the air filter (7) is a disc filter.

10. The apparatus for media transfer of claim 1, wherein: further comprises a transfer bag (10), and the liquid conduit (5) is connected to the transfer bag (10).