CN208233725U - A kind of cell culture bottle cap optimizing structure - Google Patents
A kind of cell culture bottle cap optimizing structure Download PDFInfo
- Publication number
- CN208233725U CN208233725U CN201820407108.6U CN201820407108U CN208233725U CN 208233725 U CN208233725 U CN 208233725U CN 201820407108 U CN201820407108 U CN 201820407108U CN 208233725 U CN208233725 U CN 208233725U
- Authority
- CN
- China
- Prior art keywords
- bottle cap
- cap
- top end
- bottle
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 18
- 238000002474 experimental method Methods 0.000 abstract description 10
- 238000012864 cross contamination Methods 0.000 abstract description 3
- 238000005070 sampling Methods 0.000 abstract description 3
- 210000003813 thumb Anatomy 0.000 abstract description 3
- 238000011109 contamination Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 4
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 4
- 239000012528 membrane Substances 0.000 description 3
- 239000004810 polytetrafluoroethylene Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
本实用新型涉及一种优化结构的细胞培养瓶瓶盖，包括瓶盖本体和顶端盖，瓶盖本体是中空的圆柱体，瓶盖本体内设置有内螺纹，顶端盖的下端面设置有与瓶盖本体配合扣紧的环形凸缘，顶端盖的边缘还设置有便于打开的凸起，与凸起相对的顶端盖另一边缘通过连接片与瓶盖本体连接，顶端盖上还设置有透气盖。本实用新型在瓶盖本体的基础上增加了顶端盖，顶端盖的边缘设置有便于打开的凸起。采用该瓶盖在实验过程中，用拇指指腹顶开顶端盖就可以进行加样和取样的操作，不需要多次旋开瓶盖本体，简化了实验操作，减少实验过程中的操作污染和交叉污染，且单手就可以打开顶端盖，操作非常方便；同时在实验十分必要时也可旋开瓶盖本体，为实验操作提供方便。
The utility model relates to a bottle cap for a cell culture bottle with an optimized structure, which comprises a bottle cap body and a top end cap. The cap body cooperates with the buckled ring flange, and the edge of the top end cap is also provided with a protrusion that is easy to open, and the other edge of the top end cap opposite to the protrusion is connected with the bottle cap body through a connecting piece, and the top end cap is also provided with a ventilating cap . The utility model adds a top end cap on the basis of the bottle cap body, and the edge of the top end cap is provided with a protrusion for easy opening. With this bottle cap, during the experiment, the operation of adding and sampling samples can be performed by opening the top cap with the pulp of the thumb, without unscrewing the bottle cap body many times, which simplifies the experimental operation and reduces operational pollution and contamination during the experiment. Cross-contamination, and the top cap can be opened with one hand, which is very convenient to operate; at the same time, the bottle cap body can also be unscrewed when the experiment is very necessary, which provides convenience for the experimental operation.
Description
技术领域technical field
本实用新型涉及一种瓶盖，特别涉及一种优化结构的细胞培养瓶瓶盖。The utility model relates to a bottle cap, in particular to a cell culture bottle cap with an optimized structure.
背景技术Background technique
现有细胞培养瓶的瓶盖与瓶口之间均是通过螺纹连接，在加样或是取样时，需要旋转扭开瓶盖。在细胞培养和收获实验操作过程中，多个步骤需要旋开瓶盖，一是旋开的盖子放置在操作台面上很容易被溅到液体(试剂、标本）或者被碰到引起污染，羊水细胞对于生长的环境要求很高，一旦污染就收获不到染色体，二是旋转开盖的操作方式需要双手操作，不能实现单手操作，给实验操作带来不便。The bottle cap and the bottle mouth of the existing cell culture bottle are all connected by threads, and the bottle cap needs to be rotated and unscrewed when sample is added or sampled. In the process of cell culture and harvesting experiments, multiple steps need to unscrew the bottle cap. First, the unscrewed cap is placed on the operating table and it is easy to be splashed with liquid (reagents, specimens) or touched to cause pollution. Amniotic fluid cells The requirements for the growth environment are very high. Once it is polluted, the chromosomes will not be harvested. Second, the operation method of rotating and opening the lid requires two-handed operation, which cannot be achieved by one-handed operation, which brings inconvenience to the experimental operation.
目前公开的专利文献中，中国实用新型专利2016214242852公开了一种细胞培养瓶瓶盖，包括瓶盖、瓶盖顶端、PTFE组培膜、瓶盖顶盖、顶盖凸锁扣、顶盖凹锁扣，细胞培养时，瓶盖拧紧的状态下，打开瓶盖顶盖即可，PTFE组培膜在保证空气交换的条件下，同时可以避免细菌以及病毒进入瓶内，达到防止污染的目的。虽然该专利设置有瓶盖顶盖，能够实现空气交换，但是因其还设置有PTFE组培膜，加样取样的过程仍然需要将整个瓶盖通过旋转的方式取下，还是没有解决旋转式瓶盖存在的容易被污染和操作不便的问题。另外该专利的瓶盖顶盖通过顶盖凸锁扣和顶盖凹锁扣配合扣紧在瓶盖上，需要打开时没有一个支点，打开不方便。In the currently published patent literature, Chinese utility model patent 2016214242852 discloses a bottle cap for cell culture bottles, including a bottle cap, a top end of the bottle cap, a PTFE tissue culture membrane, a top cover of the bottle cap, a convex lock of the top cover, and a concave lock of the top cover. Button, during cell culture, when the bottle cap is tightened, just open the top cap of the bottle cap. The PTFE tissue culture membrane can prevent bacteria and viruses from entering the bottle under the condition of ensuring air exchange, so as to prevent pollution. Although this patent is provided with a bottle cap top cover, which can realize air exchange, but because it is also provided with a PTFE tissue culture membrane, the process of adding and sampling still needs to remove the entire bottle cap by rotating it, and there is still no solution for the rotary bottle. The problem that the cover exists is easy to be polluted and inconvenient to operate. In addition, the bottle cap top cover of this patent is fastened on the bottle cap through the cooperation of the top cover convex lock and the top cover concave lock, and there is no fulcrum when it needs to be opened, which is inconvenient to open.
发明内容Contents of the invention
本实用新型要解决的技术问题是：提供一种优化结构的细胞培养瓶瓶盖，采用该瓶盖在实验过程中能简化实验操作（避免多次旋开盖子），减少实验过程中的操作污染和交叉污染，且单手就可以打开瓶盖，方便实验操作，解决了上述现有技术存在的问题。The technical problem to be solved by the utility model is to provide a cell culture bottle cap with an optimized structure, which can simplify the experimental operation (avoid unscrewing the cap multiple times) and reduce operational pollution during the experimental process. and cross-contamination, and the bottle cap can be opened with one hand, which is convenient for experimental operation and solves the above-mentioned problems in the prior art.
解决上述技术问题的技术方案是：一种优化结构的细胞培养瓶瓶盖，包括瓶盖本体和顶端盖，所述的瓶盖本体是中空的圆柱体，瓶盖本体内设置有内螺纹，顶端盖的下端面设置有与瓶盖本体配合扣紧的环形凸缘，顶端盖的边缘还设置有便于打开的凸起，与凸起相对的顶端盖另一边缘通过连接片与瓶盖本体连接，所述顶端盖上还设置有透气盖。The technical solution for solving the above-mentioned technical problems is: a cell culture bottle cap with an optimized structure, including a bottle cap body and a top end cap, the bottle cap body is a hollow cylinder, an internal thread is arranged in the bottle cap body, and the top end cap The lower end surface of the cap is provided with an annular flange that cooperates with the bottle cap body, and the edge of the top end cap is also provided with a protrusion that is easy to open. The other edge of the top end cap opposite to the protrusion is connected to the bottle cap body through a connecting piece. A breathable cover is also arranged on the top end cover.
所述凸起的形状为半圆形。The shape of the protrusion is a semicircle.
由于采用上述技术方案，本实用新型具有以下有益效果：Due to the adoption of the above technical solution, the utility model has the following beneficial effects:
1、本实用新型在瓶盖本体的基础上增加了顶端盖，顶端盖的边缘设置有便于打开的凸起。采用该瓶盖在实验过程中，用拇指指腹顶开顶端盖就可以进行加样和取样的操作，不需要多次旋开瓶盖本体，简化了实验操作，减少实验过程中的操作污染和交叉污染，且单手就可以打开顶端盖，操作非常方便。1. The utility model adds a top end cap on the basis of the bottle cap body, and the edge of the top end cap is provided with a protrusion for easy opening. With this bottle cap, during the experiment, the operation of adding and sampling samples can be performed by opening the top cap with the pulp of the thumb, without unscrewing the bottle cap body many times, which simplifies the experimental operation and reduces operational pollution and contamination during the experiment. Cross-contamination, and the top cover can be opened with one hand, which is very convenient to operate.
2、同时在实验十分必要时也可旋开瓶盖本体，为实验操作提供方便。2. At the same time, when the experiment is very necessary, the body of the bottle cap can also be unscrewed to provide convenience for the experiment operation.
3、与上述专利《一种细胞培养瓶瓶》相比，本实用新型瓶盖本体是完全敞开的，并没有用ptfe膜封住，接种时不用旋开瓶盖本体添加培养液，而是用拇指指腹顶开顶端盖，直接伸进试管加入培养液，这一设计正好用于实验培养第七天细胞换液的实验操作，羊水细胞培养的第七天需要将原来接种时加入的培养液倒出来再加入新的培养液，在倒出培养液的过程中，需要旋开盖子，而旋开的盖子暴露在空气中容易引起感染，而本实用新型的设计就正好可以避免这个问题，不需要旋开盖子，只需要顶开顶端盖伸进无菌吸管将原来培养液吸出，加入新的培养液继续生长。同时在实验收获这一步骤，加入胰酶消化贴壁细胞时，加入胰酶和吸尽被消化的细胞时减少了来回旋开盖子的操作。另外，本实用新型顶端盖与瓶盖本体之间是通过连接片连接，顶端盖可以尽可能地往后仰，方便在实验过程中吸管的伸进和伸出。3. Compared with the above-mentioned patent "a cell culture bottle bottle", the bottle cap body of the utility model is completely open, and is not sealed with a ptfe film. When inoculating, it is not necessary to unscrew the bottle cap body to add culture medium, but to use Open the top cover with the pulp of the thumb, and directly extend into the test tube to add the culture medium. This design is just suitable for the experimental operation of changing the medium of the cells on the seventh day of the experimental culture. Pour it out and then add new culture solution. In the process of pouring out the culture solution, you need to unscrew the cover, and the unscrewed cover is exposed to the air, which is easy to cause infection, and the design of the utility model can just avoid this problem. It is necessary to unscrew the cover, and only need to open the top cover and insert a sterile straw to suck out the original culture solution, and then add new culture solution to continue growth. At the same time, in the harvesting step of the experiment, when adding trypsin to digest the adherent cells, the operation of unscrewing the lid back and forth was reduced when adding trypsin and aspirating the digested cells. In addition, the top end cap of the present invention is connected with the bottle cap body through a connecting piece, and the top end cap can be tilted back as far as possible, which facilitates the insertion and extension of the straw during the experiment.
下面，结合附图和实施例对本实用新型之一种优化结构的细胞培养瓶瓶盖的技术特征作进一步的说明。Below, the technical features of a cell culture bottle cap with an optimized structure of the present invention will be further described in conjunction with the accompanying drawings and examples.
附图说明Description of drawings
图1：本实用新型之一种优化结构的细胞培养瓶瓶盖立体图之一。Fig. 1: One of the three-dimensional views of the cap of the cell culture bottle with an optimized structure of the utility model.
图2：本实用新型之一种优化结构的细胞培养瓶瓶盖立体图之二。Figure 2: The second perspective view of a cell culture bottle cap with an optimized structure of the utility model.
图3：本实用新型使用状态图。Fig. 3: The usage state diagram of the utility model.
图中：1-瓶盖本体，2-连接片，3-顶端盖，4-环形凸缘，5-凸起，6-透气盖。In the figure: 1-bottle cap body, 2-connecting piece, 3-top end cap, 4-ring flange, 5-protrusion, 6-breathable cap.
P表示细胞培养瓶瓶体。P represents the body of the cell culture flask.
具体实施方式Detailed ways
实施例1：一种优化结构的细胞培养瓶瓶盖，如图1-图3所示，包括瓶盖本体1和顶端盖3，所述的瓶盖本体是中空的圆柱体，瓶盖本体内设置有与细胞培养瓶瓶体连接的内螺纹，顶端盖3的下端面设置有与瓶盖本体配合扣紧的环形凸缘4，顶端盖的边缘还设置有便于打开的凸起5，与凸起相对的顶端盖另一边缘通过连接片2与瓶盖本体1连接，所述顶端盖上还设置有透气盖6。Example 1: A cell culture bottle cap with an optimized structure, as shown in Figures 1-3, includes a bottle cap body 1 and a top end cap 3, the bottle cap body is a hollow cylinder, and the body of the bottle cap An internal thread connected with the cell culture bottle body is provided, and the lower end surface of the top end cap 3 is provided with an annular flange 4 that is fastened with the bottle cap body, and the edge of the top end cap is also provided with a protrusion 5 that is easy to open. The other edge of the opposite top end cap is connected to the bottle cap body 1 through a connecting piece 2, and a vent cap 6 is also arranged on the top end cap.
本实施例中，所述凸起5的形状为半圆形。作为一种变换，所述的凸起5的形状还可以根据实际情况变化。In this embodiment, the shape of the protrusion 5 is a semicircle. As a change, the shape of the protrusion 5 can also be changed according to actual conditions.
本实用新型所述的透气盖6的具体结构与现在技术相同，此处不再赘述。The specific structure of the breathable cover 6 described in the utility model is the same as that of the prior art, and will not be repeated here.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820407108.6U CN208233725U (en) | 2018-03-23 | 2018-03-23 | A kind of cell culture bottle cap optimizing structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820407108.6U CN208233725U (en) | 2018-03-23 | 2018-03-23 | A kind of cell culture bottle cap optimizing structure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208233725U true CN208233725U (en) | 2018-12-14 |
Family
ID=64585872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820407108.6U Expired - Fee Related CN208233725U (en) | 2018-03-23 | 2018-03-23 | A kind of cell culture bottle cap optimizing structure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208233725U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023151260A1 (en) * | 2022-02-11 | 2023-08-17 | 辛普森国际生命科技(天津)有限公司 | Cell culture flask |
-
2018
- 2018-03-23 CN CN201820407108.6U patent/CN208233725U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023151260A1 (en) * | 2022-02-11 | 2023-08-17 | 辛普森国际生命科技(天津)有限公司 | Cell culture flask |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8822209B2 (en) | Disposable spinner flask | |
| CN209854174U (en) | Cell culture device for biotechnology | |
| CN205329034U (en) | A culture dish for microbial growth | |
| CN212159203U (en) | Sampling device for liquid repeatable sterilization | |
| CN211292242U (en) | Sputum liquefaction anti-pollution sputum cup | |
| CN217973236U (en) | Dropper for embryo culture | |
| CN219699972U (en) | Sample collection reagent pipe | |
| CN216919285U (en) | Anti-pollution cell culture tube with easy-open cover | |
| CN218989234U (en) | Device for transferring culture medium | |
| CN212476730U (en) | A sampling mechanism and virus sampling tube | |
| CN216584980U (en) | A throat swab collection sample sampler | |
| CN211999763U (en) | a cell culture flask | |
| CN211129304U (en) | A plant material disinfection bottle | |
| CN217516937U (en) | Shake bottle fermentation auxiliary mixing device for microbiological experiments | |
| CN221701519U (en) | Cell culture bottle convenient to change gasket | |
| CN211870165U (en) | An easy-to-open inner cap of a chemical reagent bottle | |
| CN214142367U (en) | Container capable of counting and preserving cells | |
| CN221918078U (en) | A sampling device for improving the detection rate of sputum microorganisms | |
| CN221558434U (en) | Reagent bottle integral type simple dropper strorage device | |
| CN221738875U (en) | Pipette tip waste bin | |
| CN207811762U (en) | Dual system gas exchanges culture bottle | |
| CN223667969U (en) | Swab collection assembly | |
| CN206563748U (en) | A kind of antibody incubation device suitable for western blot test | |
| CN221368839U (en) | Blood glucose medical waste collecting and separating device | |
| CN223687105U (en) | A specimen bottle for collecting bronchoalveolar lavage fluid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181214 |