CN213388658U - Bacterial group phase culture reagent bottle - Google Patents
Bacterial group phase culture reagent bottle Download PDFInfo
- Publication number
- CN213388658U CN213388658U CN202022028929.9U CN202022028929U CN213388658U CN 213388658 U CN213388658 U CN 213388658U CN 202022028929 U CN202022028929 U CN 202022028929U CN 213388658 U CN213388658 U CN 213388658U
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- Prior art keywords
- bottle
- culture
- glass conduit
- glass
- conduit
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 17
- 230000001580 bacterial effect Effects 0.000 title description 5
- 239000011521 glass Substances 0.000 claims abstract description 43
- 241000894006 Bacteria Species 0.000 claims abstract description 24
- 238000007789 sealing Methods 0.000 claims abstract description 17
- 230000001681 protective effect Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- 239000003292 glue Substances 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 description 6
- 238000005070 sampling Methods 0.000 description 3
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011012 sanitization Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a bacteria group phase culture reagent bottle, which comprises a culture bottle, wherein a glass conduit A is arranged in the middle of one side of the culture bottle, one side of the glass conduit A extends to the bottom inside the culture bottle, a rubber cap is arranged at the top of the glass conduit A, a glass conduit B is arranged in the middle of the bottom of the glass conduit A, a sealing cover is arranged at the bottom of the glass conduit B, an air suction opening is arranged at the top of one side of the culture bottle, which is close to the glass conduit A, a connecting opening is arranged at one side of the culture bottle, which is far away from the glass conduit A, a sealing bag is arranged at one side of the connecting opening, and a bottle bottom is arranged at the; the utility model discloses a glue under the cooperation of cap rubber, glass pipe A, glass pipe B, sealed lid, be convenient for take a sample to the reagent of inside cultivation, need not carry out loaded down with trivial details operation, the step is simple, can not pollute inside bacterium simultaneously.
Description
Technical Field
The utility model relates to a bacteria culture bottle technical field specifically is a bacteria mass phase culture reagent bottle.
Background
The bacterial culture technology is an important technology in the field of biological engineering, while a culture bottle is one of basic containers for bacterial culture, in the traditional culture method, when the growth state of bacteria in a liquid culture medium is monitored at any time or the condition that engineering bacteria express target protein is measured, samples need to be taken from the culture bottle at any time in the culture process;
the prior device has the following defects:
1. the closed culture bottle mouth is opened during sampling operation, and the bottle mouth is sterilized and closed after sampling is finished, so that the operation is relatively complicated, and secondary pollution is easily caused.
2. When anaerobic bacteria are cultured, air in the bottle needs to be evacuated to change the interior of the bottle into a vacuum state, but the culture bottle cannot observe whether the interior of the bottle is in the vacuum state, and if the air is left in the bottle, the culture of the bacteria is influenced.
3. The inside of the culture bottle needs to be cleaned after the bacteria are cultured every time, most of the existing culture bottles are difficult to clean, and the next experiment can be influenced if the existing culture bottles are not cleaned completely.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a bacteria mass phase culture reagent bottle, propose in solving above-mentioned background art and open the confined cultivation bottleneck when the sampling operation, the bottleneck of disinfecting again after the sample ends and seal, the operation is loaded down with trivial details relatively, and cause secondary pollution easily, need manage to find time the air in the bottle when cultivateing anaerobic bacteria, make inside become vacuum state, but whether be vacuum state in the bottle can't be observed to the blake bottle, if there is the air in the bottle, can influence the cultivation of bacterium, all need wash the inside of blake bottle after having cultivateed the bacterium at every turn, current blake bottle most all is difficult to wash, can influence the problem of next experiment if not the sanitization.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a bacteria mass phase culture reagent bottle, includes the blake bottle, the centre of blake bottle one side is provided with glass pipe A, one side of glass pipe A extends to the inside bottom of blake bottle, glass pipe A's top is provided with glues the cap, the centre of glass pipe A bottom is provided with glass pipe B, glass pipe B's bottom is provided with sealed lid, the blake bottle is close to the top of glass pipe A one side and is provided with the extraction opening, one side that glass pipe A was kept away from to the blake bottle is provided with the connector, one side of connector is provided with the seal bag, the bottom of blake bottle is provided with at the bottom of the bottle, the bottom of blake bottle both sides and the top of bottle bottom both sides are provided with the snap ring, the top of.
Preferably, the top at the bottom of the bottle is provided with a sealing ring, the bottom of the culture bottle is provided with a groove, and the groove and the sealing ring are mutually matched.
Preferably, one side of one end of the culture bottle is provided with a scale mark.
Preferably, the top of the culture bottle is provided with a protective cover.
Preferably, the rubber cap is made of a rubber material.
Preferably, the bottom of the outer side of the glass conduit B and the inner side of the sealing cover are both provided with a thread structure.
Compared with the prior art, the beneficial effects of the utility model are that: the bacteria group phase culture reagent bottle;
1. by matching the rubber cap, the glass conduit A, the glass conduit B and the sealing cover, the reagent cultured inside can be conveniently sampled, complicated operation is not needed, the steps are simple, and meanwhile, the bacteria inside cannot be polluted;
2. under the matching of the connecting port and the sealing bag, whether the interior is vacuumized can be observed during vacuumizing, so that the condition that air exists in the interior and the anaerobic bacteria are cultured is prevented from being influenced;
3. under the cooperation through snap ring, bolt, bottle end, can pull down the bottle end, conveniently wash inside, improve abluent effect, can use the bottle end as the culture dish when pulling down the bottle end again.
Drawings
Fig. 1 is a front sectional view of the present invention;
FIG. 2 is a front view of the present invention;
fig. 3 is an enlarged schematic view of a structure in fig. 1 according to the present invention.
In the figure: 1. a culture bottle; 2. a connecting port; 3. sealing the bag; 4. an air extraction opening; 5. a rubber cap; 6. a glass conduit A; 7. a glass conduit B; 8. a snap ring; 9. a bolt; 10. a bottle bottom; 11. and (7) sealing the cover.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides an embodiment: the utility model provides a bacterial colony culture reagent bottle, including blake bottle 1, the centre of blake bottle 1 one side is provided with glass pipe A6, one side of glass pipe A6 extends to the inside bottom of blake bottle 1, glass pipe A6's top is provided with rubber cap 5, the centre of glass pipe A6 bottom is provided with glass pipe B7, glass pipe B7's bottom is provided with sealed lid 11, blake bottle 1 is close to the top of glass pipe A6 one side and is provided with extraction opening 4, blake bottle 1 keeps away from one side of glass pipe A6 and is provided with connector 2, one side of connector 2 is provided with sealed bag 3, blake bottle 1's bottom is provided with bottle end 10, the bottom of blake bottle 1 both sides and the top of bottle end 10 both sides are provided with snap ring 8, the top of snap ring 8 is provided with.
Further, the top of bottle end 10 is provided with the sealing ring, and the bottom of blake bottle 1 is provided with the recess, and recess and the mutual adaptation of sealing ring improve the seal, prevent that the condition of weeping and gas leakage from taking place to take place.
Furthermore, one side of one end of the culture bottle 1 is provided with scale marks, so that the height of the liquid level can be observed conveniently.
Furthermore, the top of blake bottle 1 is provided with the protective cover, conveniently puts into the culture solution, prevents gas leakage.
Further, the rubber cap 5 is made of a rubber material, and the rubber material is good in elasticity.
Furthermore, the bottom of the outer side of the glass guide pipe B7 and the inner side of the sealing cover 11 are both provided with thread structures, so that the fixing effect is good.
The working principle is as follows: during the use, will open the protective cover earlier and put into blake bottle 1 with the bacterium and the culture solution that need cultivate, cover the protective cover again, extract the inside air of blake bottle 1 through extraction opening 4, observe whether the state of sealed bag 3 is flat state, if for flat state description inside air has been extracted totally, cultivate for a period of time back, through pressing the inside bacterium of cultivateing of rubber cap 5 extraction blake bottle 1, the reagent of cultivateing the bacterium also can flow into the inside of glass pipe B7 when the extraction, then open sealed lid 11 culture solution and will follow glass pipe B7 and flow out, take a sample, use up the back and rotate bolt 9 and remove the fixed to snap ring 8, then open the bottle end 10, clear up the inside of blake bottle 1, can place the bacterium in the bottle end 10 again simultaneously and observe.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are merely for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Claims (6)
1. A bacteria group phase culture reagent bottle comprises a culture bottle (1), and is characterized in that: the middle of one side of the culture bottle (1) is provided with a glass conduit A (6), one side of the glass conduit A (6) extends to the bottom inside the culture bottle (1), the top of the glass conduit A (6) is provided with a rubber cap (5), the middle of the bottom of the glass conduit A (6) is provided with a glass conduit B (7), the bottom of the glass conduit B (7) is provided with a sealing cover (11), the top of one side, close to the glass conduit A (6), of the culture bottle (1) is provided with an air suction opening (4), one side, far away from the glass conduit A (6), of the culture bottle (1) is provided with a connecting opening (2), one side of the connecting opening (2) is provided with a sealing bag (3), the bottom of the culture bottle (1) is provided with a bottle bottom (10), the bottoms of two sides of the culture bottle (1) and the tops of two sides of the bottle bottom (10, and a bolt (9) is arranged at the top of the clamping ring (8).
2. The bacteria mass culture reagent bottle of claim 1, wherein: the top of the bottle bottom (10) is provided with a sealing ring, the bottom of the culture bottle (1) is provided with a groove, and the groove and the sealing ring are matched with each other.
3. The bacteria mass culture reagent bottle of claim 1, wherein: one side of one end of the culture bottle (1) is provided with scale marks.
4. The bacteria mass culture reagent bottle of claim 1, wherein: the top of blake bottle (1) is provided with the protective cover.
5. The bacteria mass culture reagent bottle of claim 1, wherein: the rubber cap (5) is made of rubber materials.
6. The bacteria mass culture reagent bottle of claim 1, wherein: the bottom of the outer side of the glass guide pipe B (7) and the inner side of the sealing cover (11) are both provided with thread structures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022028929.9U CN213388658U (en) | 2020-09-16 | 2020-09-16 | Bacterial group phase culture reagent bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022028929.9U CN213388658U (en) | 2020-09-16 | 2020-09-16 | Bacterial group phase culture reagent bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213388658U true CN213388658U (en) | 2021-06-08 |
Family
ID=76179325
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022028929.9U Active CN213388658U (en) | 2020-09-16 | 2020-09-16 | Bacterial group phase culture reagent bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213388658U (en) |
-
2020
- 2020-09-16 CN CN202022028929.9U patent/CN213388658U/en active Active
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