CN206328393U - A kind of centrifuge tube for cleaning and digesting tissue - Google Patents
A kind of centrifuge tube for cleaning and digesting tissue Download PDFInfo
- Publication number
- CN206328393U CN206328393U CN201621451536.6U CN201621451536U CN206328393U CN 206328393 U CN206328393 U CN 206328393U CN 201621451536 U CN201621451536 U CN 201621451536U CN 206328393 U CN206328393 U CN 206328393U
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- CN
- China
- Prior art keywords
- centrifuge tube
- cellular filter
- drain pipe
- tube
- tissue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The utility model discloses a kind of centrifuge tube for cleaning and digesting tissue, including body, drain pipe, cellular filter；The cellular filter is removably installed in the body, by the body be divided into above and below two parts, the cellular filter be located at away from the tube mouth 1/1st to three/2nd at；Its bottom connects the drain pipe；The port of export of the drain pipe is provided with convex port, and the nut of rotating folding is provided with the convex port.As long as the utility model can complete to clean and digest organizational process using 1 centrifuge tube, the loss of centrifuge tube is substantially reduced, cost is reduced, and operation is easier, the operating time is saved, while reducing contaminated probability in operating process.
Description
Technical field
The utility model is related to Bioexperiment instrument field, more particularly to a kind of centrifuge tube for cleaning and digesting tissue.
Background technology
Primary cell (primary cell) refers to tissue (such as people's tissue, mouse tissue, rat tissue and the rabbit from body
Tissue etc.) obtain individual cells through protease or other methods and be simulated the cell of body culture in vitro.Usual egg
Before white enzymic digestion, tissue block is cleaned, connective tissue and watery blood outside removal, and then tissue is shredded, to carry
The efficiency of high protein enzymic digestion.In the process, in order to be eliminated as much as haemocyte, the centrifuge tube of more than 2 can be used, is centrifuged
Pipe belongs to easily-consumed products, and greatly waste, and add the probability of pollution can be caused every time by changing.
Utility model content
In order to solve the above technical problems, the utility model provides a kind of centrifuge tube for cleaning and digesting tissue, using pipe
The structure that body is combined with cellular filter, solving needs to use multiple centrifuge tubes in cleaning and digesting organizational process and exists dirty
The problem of contaminating probability.
To reach above-mentioned purpose, the technical solution of the utility model is as follows：A kind of centrifuge tube for cleaning and digesting tissue, bag
Include body, drain pipe, cellular filter；The cellular filter is removably installed in the body, and the body is divided into
Lower two parts, the cellular filter is located at away from the tube mouth 1/1st to three/2nd；Its bottom connection
The drain pipe；The port of export of the drain pipe is provided with convex port, and rotating folding is provided with the convex port
Nut.
It is preferred that, the aperture of the cellular filter is 50-100 μm.
It is preferred that, the aperture of the cellular filter is 70 μm.
It is preferred that, the inboard wall of tube body is provided with the prominent circle of positioning for installing the cellular filter；In the cell
The periphery of filter is provided with the sealing ring coordinated with the prominent circle of the positioning.
It is preferred that, U-shaped breach is being provided with tube wall at the opening of the body, is being provided with and carries on the cellular filter
Bar, described shadoof one end is connected with the cellular filter, and the other end stretches out from the U-shaped breach；The shadoof lacks in the U-shaped
Mouth correspondence position is provided with the sealing-plug being adapted to the U-shaped breach.
It is preferred that, the drain pipe is flexible pipe, and the nearly tube end of the drain pipe is provided with pipe clamp.
It is preferred that, the drain pipe is glass tube, and the nearly tube end of the drain pipe is provided with glass cock.
It is preferred that, its bottom outer wall is provided with support feet, and the support feet is 3, centered on the body
In angular distribution.
It is preferred that, the body, equipped with 2 lids, is respectively with U-shaped plug lid and without U-shaped plug lid；It is described not
With the body fitted seal when band U-shaped plug lid is for the body installation cellular filter；The band U-shaped plug lid is used
With the body fitted seal when body has dismantled the cellular filter.
By above-mentioned technical proposal, the beneficial effect of technical solutions of the utility model is：As long as the utility model uses 1
Centrifuge tube can complete to clean and digest organizational process, substantially reduce the loss of centrifuge tube, reduce cost, and operate more
Simplicity, saves the operating time, while reducing contaminated probability in operating process.
Brief description of the drawings
, below will be to embodiment in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
Or the accompanying drawing to be used needed for description of the prior art is briefly described, it should be apparent that, drawings in the following description are only
It is some embodiments of the present utility model, for those of ordinary skill in the art, is not paying the premise of creative work
Under, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the structural representation of cellular filter of the utility model with shadoof.
Fig. 3 is the structural representation of the utility model body.
Fig. 4 is structural representation of the utility model with U-shaped plug lid.
Numeral and the corresponding component title represented by letter in figure：
1. the drain pipe of 2. cellular filter of body 3.
4. the sealing ring of 5. nut of convex port 6.
7. the sealing-plug of 8. glass cock of shadoof 9.
10. the prominent support feet of circle 11.U v notch vs 12. of positioning
13. band U-shaped plug lid
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is carried out
Clearly and completely describe, it is clear that described embodiment is only a part of embodiment of the utility model, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belongs to the scope of the utility model protection.
Embodiment
With reference to Fig. 1 to Fig. 4, the utility model discloses it is a kind of clean and digestion tissue centrifuge tube, including body 1, go out
Liquid pipe 3, cellular filter 2.Cellular filter 2 is removably installed in body 1, by body 1 be divided into above and below two parts, cellular filter
2 are located at away from the mouth of pipe 1/1st to three/2nd of body 1；The aperture of cellular filter 2 is 50-100 μm, preferably 70 μm.Pipe
The bottom of body 1 connects drain pipe 3；The port of export of drain pipe 3 is provided with convex port 4, and rotating folding is provided with convex port 4
Nut 5.In order to preferably control drain pipe 3, when drain pipe 3 uses flexible pipe, the nearly end of body 1 of drain pipe 3 is provided with pipe clamp；
Such as Fig. 1, when drain pipe 3 uses glass tube, the nearly end of body 1 of drain pipe 3 is provided with glass cock 8.Outside such as Fig. 3, the bottom of body 1
Wall can also install support feet 12, and support feet 12 is 3, be in angular distribution centered on body 1.So centrifuge tube just can be
In the case of there is no external auxiliary apparatus, realize to erect and place, very big facility is provided for operating process.
The dismountable mounting means of cellular filter 2, can specifically use following structures：Such as Fig. 1, set in the inwall of body 1
Have for installing the prominent circle 10 of the positioning of cellular filter 2；The sealing coordinated with the prominent circle 10 of positioning is provided with the periphery of cellular filter 2
Circle 6.Such as Fig. 2, U-shaped breach is being provided with tube wall at the opening of body 1, shadoof 7, shadoof 7 one are installed on cellular filter 2
End is connected with cellular filter 2, and the other end stretches out from U-shaped breach；Shadoof 7 is provided with suitable with U-shaped breach in U-shaped breach correspondence position
The sealing-plug 9 matched somebody with somebody.
In operation, it there may come a time when to need to dismantle lower cellular filter 2, therefore body 1 is preferably provided with 2 lids,
Respectively with U-shaped plug lid 13 and without U-shaped plug lid；It is used for when body 1 installs cellular filter 2 and manages without U-shaped plug lid
The fitted seal of body 1.Such as Fig. 4, band U-shaped plug lid 13 is used for when body 1 has dismantled cellular filter 2 and the fitted seal of body 1.
Below by taking stomach fat as an example, illustrate use process of the present utility model：
1st, drain pipe 3 is closed using glass cock 8 or pipe clamp, belly is sterile to take fat, is put into body 1, is placed in
On cellular filter 2；
2nd, liposuction liquid is with 1000 revs/min of brief centrifugations, adipose tissue in the top of cellular filter 2, lower section be tissue fluid and
Watery blood；
3rd, pipe clamp or glass cock 8 are clamped, nut 5 is opened, pipe clamp or glass cock 8 is unclamped, tissue fluid and watery blood is released,
Cover nut 5；
4th, PBS repeated washings are added, until the liquid clear of lower section is colourless, nut 5 is opened, releases liquid, cover
Nut 5；
5th, the NTx enzyme of addition 0.075%, 37 DEG C of waters bath with thermostatic control digestion, and shaken in digestion process every 5 minutes
Once, used after 30 minutes in the DMEM/F12 culture mediums containing 10% serum and clostridiopetidase A, 1000rpm/min is centrifuged 10 minutes, moved
Go out cellular filter 2.With the cell mass of the bottom of culture medium suspension body 1 containing serum, it is taped against in 10cm culture dish, is placed in 37
DEG C, cultivated in the incubator of the moisture-saturated containing 5%CO2.
By above-mentioned specific embodiment, the beneficial effects of the utility model are：As long as the utility model uses 1 centrifuge tube
It can complete to clean and digest organizational process, substantially reduce the loss of centrifuge tube, reduce cost, and operation is easier,
The operating time is saved, while reducing contaminated probability in operating process.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or new using this practicality
Type.A variety of modifications to these embodiments will be apparent for those skilled in the art, determine herein
The General Principle of justice can in other embodiments be realized in the case where not departing from spirit or scope of the present utility model.Cause
This, the utility model is not intended to be limited to the embodiments shown herein, and is to fit to and principles disclosed herein
The most wide scope consistent with features of novelty.
Claims (9)
1. a kind of centrifuge tube for cleaning and digesting tissue, it is characterised in that including body, drain pipe, cellular filter；The cell
Filter is removably installed in the body, by the body be divided into above and below two parts, the cellular filter be located at away from described
At tube mouth 1/1st to three/2nd；Its bottom connects the drain pipe；The port of export peace of the drain pipe
Equipped with convex port, the nut of rotating folding is provided with the convex port.
2. cleaning according to claim 1 and the centrifuge tube of digestion tissue, it is characterised in that the aperture of the cellular filter
For 50-100 μm.
3. cleaning according to claim 1 and the centrifuge tube of digestion tissue, it is characterised in that the aperture of the cellular filter
For 70 μm.
4. cleaning according to claim 1 and the centrifuge tube of digestion tissue, it is characterised in that set in the inboard wall of tube body
Have for installing the prominent circle of the positioning of the cellular filter；It is provided with and coordinates with the prominent circle of the positioning in the periphery of the cellular filter
Sealing ring.
5. cleaning according to claim 1 and the centrifuge tube of digestion tissue, it is characterised in that at the opening of the body
U-shaped breach is provided with tube wall, shadoof is installed on the cellular filter, described shadoof one end connects with the cellular filter
Connect, the other end stretches out from the U-shaped breach；The shadoof is provided with suitable with the U-shaped breach in the U-shaped breach correspondence position
The sealing-plug matched somebody with somebody.
6. cleaning according to claim 1 and the centrifuge tube of digestion tissue, it is characterised in that the drain pipe is flexible pipe,
The nearly tube end of the drain pipe is provided with pipe clamp.
7. cleaning according to claim 1 and the centrifuge tube of digestion tissue, it is characterised in that the drain pipe is glass
Pipe, glass cock is provided with the nearly tube end of the drain pipe.
8. cleaning according to claim 1 and the centrifuge tube of digestion tissue, it is characterised in that its bottom outer wall peace
Equipped with support feet, the support feet is 3, is in angular distribution centered on the body.
9. cleaning according to claim 1 and the centrifuge tube of digestion tissue, it is characterised in that the body is equipped with 2
Lid, is respectively with U-shaped plug lid and without U-shaped plug lid；It is described to install described thin for the body without U-shaped plug lid
With the body fitted seal during born of the same parents' filter；When the band U-shaped plug lid has dismantled the cellular filter for the body with
The body fitted seal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621451536.6U CN206328393U (en) | 2016-12-28 | 2016-12-28 | A kind of centrifuge tube for cleaning and digesting tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621451536.6U CN206328393U (en) | 2016-12-28 | 2016-12-28 | A kind of centrifuge tube for cleaning and digesting tissue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206328393U true CN206328393U (en) | 2017-07-14 |
Family
ID=59293243
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621451536.6U Expired - Fee Related CN206328393U (en) | 2016-12-28 | 2016-12-28 | A kind of centrifuge tube for cleaning and digesting tissue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206328393U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117305078A (en) * | 2023-09-25 | 2023-12-29 | 成都赛恩吉诺生物科技有限公司 | Tissue dissociation method |
-
2016
- 2016-12-28 CN CN201621451536.6U patent/CN206328393U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117305078A (en) * | 2023-09-25 | 2023-12-29 | 成都赛恩吉诺生物科技有限公司 | Tissue dissociation method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200522 Address after: 215000 Room 204 and 205, building A5, bio nano Park, 218 Xinghu street, Suzhou Industrial Park, Jiangsu Province Patentee after: Palmer biomedical testing (Suzhou) Co.,Ltd. Address before: Xinghu Street Industrial Park of Suzhou city in Jiangsu province 215000 BioBAY No. 218 building A5 room 201-206 Patentee before: HOPE BIOLOGICAL TECHNOLOGY (SUZHOU) Co.,Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170714 Termination date: 20211228 |