CN110564599B - Anti-pollution formula bacteria culture device of clinical laboratory - Google Patents

Anti-pollution formula bacteria culture device of clinical laboratory Download PDF

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CN110564599B
CN110564599B CN201910904682.1A CN201910904682A CN110564599B CN 110564599 B CN110564599 B CN 110564599B CN 201910904682 A CN201910904682 A CN 201910904682A CN 110564599 B CN110564599 B CN 110564599B
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shell
chassis
dish body
bacteria culture
bacteria
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CN110564599A (en
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郭旭昌
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Xi'an No4 Hospital
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Xi'an No4 Hospital
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination

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  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
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Abstract

The invention belongs to the technical field of bacteria culture, and particularly relates to an anti-pollution bacteria culture device for a clinical laboratory, which comprises a dish body and a cover shell above the dish body, wherein a base plate is arranged below the dish body, a linking shell is arranged between the dish body and the base plate, the linking shell and the dish body are of an integrated structure, a gauze disc and a positioning disc are respectively arranged on the upper surface and the lower surface of the base plate, the positioning disc at the bottom of the base plate can be screwed off from a through groove, so that bacteria culture liquid in the linking shell can be quickly discharged along a flowing warp gauze disc and the through groove, a small cap above a connecting column is opened, the culture liquid is re-injected into the dish body by using tools such as an external dropper or a needle cylinder, the problem that the original bacteria culture dish is easily polluted by bacteria when the culture liquid in the interior is discharged and replaced is solved, the quality and the efficiency of the bacteria culture are ensured, and the number of ventilation holes can be controlled by moving a second plate shell, so that the speed of the air exchange with the exterior in the bacteria culture process is effectively controlled.

Description

Anti-pollution formula bacteria culture device of clinical laboratory
Technical Field
The invention belongs to the technical field of bacterial culture, and particularly relates to an anti-pollution type bacterial culture device for a clinical laboratory.
Background
The bacteria culture is a technology for making bacteria grow and propagate by an artificial method, the bacteria are extremely wide in distribution, large in quantity and multiple in types in the nature, can benefit mankind and can also become a cause of diseases, most of the bacteria can be cultured by the artificial method, namely, the bacteria are inoculated on a culture medium to make the bacteria grow and propagate, the cultured bacteria are used for research, identification and application, the bacteria culture is a complex technology, the bacteria are everywhere, so the whole culture process needs to be carried out according to the requirement of aseptic operation when the culture medium is prepared, otherwise, the external bacteria pollute a specimen and cause wrong results, once the cultured pathogenic bacteria pollute the environment, cross infection can be caused, in the existing technical means, a bacteria culture dish is usually used for correspondingly culturing the bacteria, but the problems of pollution type still exist, wherein the common problem is that a culture dish cover needs to be opened when an inspection department discharges and replaces a bacteria culture solution, in the process, other bacteria are often polluted, the problems that the existing bacteria culture dish is used in the process, the gas exchange efficiency is not correspondingly reduced, and the gas exchange efficiency is reduced.
Disclosure of Invention
To solve the problems raised in the background art described above. The invention provides an anti-pollution type bacteria culture device for a clinical laboratory, which has the characteristics of preventing pollution caused by replacing a bacteria culture solution and having more practical functions.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides an anti-pollution formula bacteria culture device of clinical laboratory, including the ware body and the lid shell of ware body top, the below of the ware body is provided with the chassis, be provided with between the ware body and the chassis and link up the shell, link up shell and ware body formula structure as an organic whole, the upper and lower surface on chassis is provided with gauze dish and positioning disk respectively, logical groove has been seted up to the inside on chassis, the annular distribution has the fixed block on the inner wall of lid shell, the chassis is on the surface with the corresponding position department butt fusion of fixed block has the stopper, the draught hole has been seted up to the upper surface of lid shell, the both sides position department of draught hole top all is provided with first shell and second shell.
Preferably, the gauze disc and the positioning disc are both connected to the base plate in a rotating mode through threads, the shape and the size of the outer portion of the gauze disc are matched with the shape and the size of the circular groove formed in the dish body, and connecting columns are integrally formed in the positions of the two sides of the upper surface of the cover shell.
Preferably, the first board shell and the cover shell are welded, the first board shell and the second board shell are mutually clamped together, the number of the second board shells is two, and the magnetic blocks are embedded on the surfaces of the two second board shells, which are in contact with each other.
Preferably, the chassis is connected with the linking shell in a clamping manner through a limiting block and a fixing block, and the upper surface of the chassis is bonded with a sealing layer which is a rubber component.
Preferably, the lower surface of the cover shell is integrally formed with a clamping edge, an edge groove is formed in the position, corresponding to the clamping edge, on the dish body, and the cover shell and the dish body are connected in a clamping mode through the clamping edge and the edge groove.
Preferably, the lower surface of the chassis is distributed with a base, and the base and the chassis are of an integrated structure.
Compared with the prior art, the invention has the beneficial effects that: in the using process, the positioning disc at the bottom of the chassis can be rotated away from the through groove, so that the bacterial culture solution in the connecting shell can be quickly discharged along the warp flow net disc and the through groove, the small cap above the connecting column is opened, the culture solution is re-injected into the dish body by using tools such as an external dropper or a needle cylinder, and the like, so that the problem that the original bacterial culture dish is easily polluted by bacteria when the internal culture solution is discharged and replaced is solved, the quality and the efficiency of bacterial culture are ensured, the number of the ventilating holes can be controlled by moving the second plate shell, the speed of the bacterial culture dish during exchange with external gas is effectively controlled, the good stability between the dish body and the cover shell is ensured under the action of the clamping edge and the side groove, and the practicability and the functionality of the bacterial culture dish are improved.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic view of the structure of the present invention;
FIG. 2 is a front view of the assembled culture dish of the present invention;
FIG. 3 is a schematic view showing the structure of the inside of the adaptor case according to the present invention;
FIG. 4 is an enlarged schematic view of the structure at A in FIG. 3;
fig. 5 is a schematic structural view of the first plate package of the present invention.
In the figure: 1. a dish body; 2. a linker shell; 3. a chassis; 4. a cover shell; 5. clamping edges; 6. a side groove; 7. a screen disc; 8. connecting columns; 9. a first plate shell; 10. a second plate shell; 11. a ventilation hole; 12. a base; 13. positioning a plate; 14. a through groove; 15. a fixed block; 16. a limiting block; 17. and (4) a sealing layer.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1-5, the present invention provides the following technical solutions: the utility model provides an anti-pollution formula bacteria culture device of clinical laboratory, lid shell 4 including the ware body 1 and the ware body 1 top, the below of the ware body 1 is provided with chassis 3, be provided with between the ware body 1 and the chassis 3 and link up shell 2, link up shell 2 and the ware body 1 formula structure as an organic whole, on chassis 3, the lower surface is provided with gauze dish 7 and positioning disk 13 respectively, logical groove 14 has been seted up to chassis 3's inside, annular distribution has fixed block 15 on the inner wall of lid shell 4, chassis 3 is on the surface with the corresponding position department butt fusion of fixed block 15 spacing block 16, venthole 11 has been seted up to the upper surface of lid shell 4, the both sides position department of venthole 11 top all is provided with first shell 9 and second shell 10, utilize corresponding liquid component and the structure of taking a breath of changing, solve original bacteria culture ware and arrange the problem that easily receives bacterial contamination when changing of internal culture liquid, speed when effectual control bacteria culture ware exchanges with outside gas, the practicality and the functional of bacteria culture ware have been increased.
Specifically, gauze dish 7 and positioning disk 13 all connect soon on chassis 3 through the screw thread, gauze dish 7 outside shape size and the inside shape size phase-match of seting up the circular slot of dish body 1, the both sides position department integrated into one piece of lid 4 upper surface has spliced pole 8, through with positioning disk 13 and chassis 3 alternate segregation, can make the quick outflow of the inside culture solution of dish body 1, make the in-process that the liquid was traded in the bacterial culture be difficult for receiving the interference of outside miscellaneous fungus, the normal clear that the liquid process was traded in the bacterial culture has been guaranteed.
Specifically, first board shell 9 and lid shell 4 looks butt fusion, and first board shell 9 and second board shell 10 looks joint are in the same place each other, and second board shell 10 is provided with two altogether, and the gomphosis has the magnetic path on the surface that two second board shells 10 contact, under the effect of magnetic path, makes two second board shells 10 closure that can be quick in the contact, through the quantity of using hand pulling second board shell 10 steerable draught hole 11 to speed when effectual control bacteria culture in-process and outside gas exchange.
Specifically, chassis 3 is connected with linking up 2 blocks of shell through stopper 16 and fixed block 15, and the upper surface bonding of chassis 3 has seal 17, and seal 17 is the rubber component, block nature and seal 17's effect between stopper 16 and the fixed block 15, makes 3 tops on chassis and the dish body 1 and link up being connected with better seal between the shell 2, has prevented overflowing of the inside culture solution of the dish body on the one hand, and on the other hand has guaranteed the normal clear of bacteria culture process.
Specifically, the lower surface integrated into one piece of lid shell 4 has card limit 5, has seted up limit groove 6 with the corresponding position department in card limit 5 on the ware body 1, and lid shell 4 and ware body 1 are connected through card limit 5 and 6 block in limit groove, and better connection stability and leakproofness have been guaranteed between the ware body 1 and the lid shell 4 to block connectivity between the two, make the bacteria culture process can not receive the interference of external environment factor.
Specifically, the lower surface distribution of chassis 3 has base 12, base 12 and chassis 3 formula structure as an organic whole, and the outside registrates of base 12 has the rubber sleeve for whole bacteria cultivation dish can not slide easily when placing on the desktop, guarantees going on that the bacteria cultivation process is stable.
The working principle and the using process of the invention are as follows: in the using process of the invention, before a worker cultures bacteria, the worker can select a gauze disc 7 with a proper aperture size according to the specific situation of the bacteria to be cultured so that the bacteria to be cultured cannot flow downwards through the gauze disc 7, then the gauze disc 7 is screwed above the chassis 3, then the positioning disc 13 is connected below the chassis 3 in the same screwing mode, the chassis 3 is clamped into the connection shell 2, the limiting block 16 on the chassis 3 is inserted into the fixing block 15 on the inner wall of the connection shell 2, the sealing layer 17 on the upper surface of the chassis 3 is in contact with the surface of the circular groove formed in the dish body 1, then corresponding bacteria and culture liquid are put into the dish body 1, the clamping edge 5 on the cover shell 4 is clamped into the side groove 6 on the dish body 1, so that the bacteria culture process in the bacteria culture dish is completed, after a period of culture, the positioning disc 13 can be screwed off from the inside of the dish body 14 by hand, at the moment, the culture liquid in the through groove 14 in the dish body 1 flows out, the bacteria along the through groove 14, the air in the air exchange needle tube 1, and the air exchange process of the air exchange needle tube shell 1, so that the air exchange of the air exchange shell can be carried out by using the air exchange tube 1, and the air exchange process of the air exchange device, and the air exchange process of the air exchange shell, wherein the air exchange tube shell 1.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art will understand that various changes, modifications and substitutions can be made without departing from the spirit and scope of the invention as defined by the appended claims. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (4)

1. The utility model provides an anti-pollution formula bacteria culture device of clinical laboratory, includes lid shell (4) of ware body (1) and ware body (1) top, its characterized in that: a chassis (3) is arranged below the dish body (1), a connecting shell (2) is arranged between the dish body (1) and the chassis (3), the connecting shell (2) and the dish body (1) are of an integral structure, a gauze disc (7) and a positioning disc (13) are respectively arranged on the upper surface and the lower surface of the chassis (3), a through groove (14) is formed in the chassis (3), fixing blocks (15) are annularly distributed on the inner wall of the cover shell (4), a limiting block (16) is welded on the surface of the chassis (3) at a position corresponding to the fixing blocks (15), an air vent (11) is formed in the upper surface of the cover shell (4), and a first plate shell (9) and a second plate shell (10) are arranged at positions on two sides above the air vent (11); the gauze disc (7) and the positioning disc (13) are both screwed on the chassis (3) through threads, the shape and size of the outer part of the gauze disc (7) are matched with the shape and size of a circular groove formed in the dish body (1), and connecting columns (8) are integrally formed at the positions of two sides of the upper surface of the cover shell (4); first board shell (9) and lid shell (4) looks butt fusion, first board shell (9) and second board shell (10) mutual block are in the same place, and second board shell (10) are provided with two altogether, and the gomphosis has the magnetic path on the surface that two second board shells (10) contacted.
2. The contamination-preventive bacteria culture apparatus for clinical laboratory according to claim 1, wherein: the chassis (3) is connected with the linking shell (2) in a clamping mode through the limiting blocks (16) and the fixing blocks (15), the upper surface of the chassis (3) is bonded with the sealing layer (17), and the sealing layer (17) is a rubber component.
3. The contamination-preventive bacteria culture apparatus for clinical laboratory according to claim 1, wherein: the lower surface of the cover shell (4) is integrally formed with a clamping edge (5), an edge groove (6) is formed in the position, corresponding to the clamping edge (5), on the dish body (1), and the cover shell (4) and the dish body (1) are connected in a clamping mode through the clamping edge (5) and the edge groove (6).
4. The contamination-preventive bacteria culture apparatus for clinical laboratory according to claim 1, wherein: the lower surface of the chassis (3) is distributed with a base (12), and the base (12) and the chassis (3) are of an integrated structure.
CN201910904682.1A 2019-09-24 2019-09-24 Anti-pollution formula bacteria culture device of clinical laboratory Active CN110564599B (en)

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CN110564599B true CN110564599B (en) 2023-01-03

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090056667A (en) * 2007-11-30 2009-06-03 코아스템(주) The cell culture apparatus and mass automatic cell culture device having it
CN103597068A (en) * 2011-03-30 2014-02-19 独立行政法人国立长寿医疗研究中心 Membrane-separation-type culture device, membrane-separation-type culture kit, stem cell separation method using same, and separation membrane
CN109456892A (en) * 2018-12-12 2019-03-12 河南医学高等专科学校 A kind of incubator of accelerated cell growth rate

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090056667A (en) * 2007-11-30 2009-06-03 코아스템(주) The cell culture apparatus and mass automatic cell culture device having it
CN103597068A (en) * 2011-03-30 2014-02-19 独立行政法人国立长寿医疗研究中心 Membrane-separation-type culture device, membrane-separation-type culture kit, stem cell separation method using same, and separation membrane
CN109456892A (en) * 2018-12-12 2019-03-12 河南医学高等专科学校 A kind of incubator of accelerated cell growth rate

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