CN109679828B

CN109679828B - Culture dish for bacterial culture and use method

Info

Publication number: CN109679828B
Application number: CN201910134134.5A
Authority: CN
Inventors: 胥振国; 吴义明; 蔡玉华
Original assignee: 胥振国
Current assignee: Hefei Technology College
Priority date: 2019-02-22
Filing date: 2019-02-22
Publication date: 2020-06-09
Anticipated expiration: 2039-02-22
Also published as: CN109679828A

Abstract

The invention discloses a culture dish for bacterial culture and a using method thereof. This culture dish and application method for bacterial culture through inlayer and outer mutual structure for liquid sample can obtain dual protection when cultivateing, even the inlayer fracture is not found, also can carry out the antiseep protection through the skin, and the parting bead can be separated the space of inlayer, and realizes four regional independent uses, thereby can be fit for the cultivation of the same kind or multiple liquid sample, improves its practicality.

Description

Culture dish for bacterial culture and use method

Technical Field

The invention relates to the technical field of bacterial culture, in particular to a culture dish for bacterial culture and a using method thereof.

Background

The culture dish is a laboratory vessel used for microorganism or cell culture, mainly comprises plastic and glass, and the glass can be used for plant materials, microorganism culture and adherent culture of animal cells; the plastic is mostly polyethylene material, has disposable and multiple use, is suitable for laboratory inoculation, streaking and bacterium separation operation, and can be used for plant material culture.

The existing liquid sample culture dish basically comprises a disc and a sealing cover, when the culture dish is used, if the condition of collision occurs, the condition of cracking and leakage easily occurs, and meanwhile, the sealing cover cannot be used for culturing some aerobic bacteria, so that the culture dish is easy to spill out when being transferred, and unnecessary troubles are brought to workers.

Disclosure of Invention

The invention aims to provide a culture dish for bacterial culture and a using method thereof, and aims to solve the problem that the traditional culture dish brings inconvenience to workers.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a culture dish for bacterial culture, includes the inlayer, be provided with the parting bead in the inlayer, be provided with the skin on the outer wall of inlayer, outer lower extreme is provided with the silica gel pad, the both sides of inlayer are provided with the elasticity card, outer both sides are provided with the draw-in groove, elasticity card joint draw-in groove.

Preferably, the inner layer is covered with a sealing cover, an annular first thread bulge is arranged on the inner wall of the sealing cover, an annular second thread bulge is arranged on the outer layer, and the first thread bulge is in threaded connection with the second thread bulge.

Preferably, the sealing cover is provided with a cover plate, and the cover plate is provided with a knob.

Preferably, the both sides of apron are provided with the recess, fixedly connected with spring in the recess, the other end fixedly connected with slider of spring, the other end fixedly connected with buckle of slider, fixedly connected with counterpoint piece on the buckle, the both sides of sealed lid are provided with the counterpoint groove, and the counterpoint piece slides and cup joints the counterpoint groove.

Preferably, the sealing cover is embedded with a lantern ring, the cover plate is fixedly connected with a sealing block, and the sealing block is sleeved with the lantern ring in a sliding mode.

Preferably, the separating strip is of a cross structure, the lower end of the separating strip is fixedly adhered to the bottom surface in the inner layer, and the upper end of the separating strip is tightly attached to the sealing cover.

Preferably, the elastic card is made of a regular hexagonal rubber material, and the shape of the clamping groove is the same as that of the elastic card and the size of the clamping groove is matched with that of the elastic card.

Preferably, the method for using the culture dish for bacterial culture is as follows:

the first step is as follows: firstly, combining the inner layer and the outer layer, and clamping the inner layer and the outer layer into the clamping groove through the elastic clamp so as to fix the inner layer and the outer layer;

the second step is that: respectively putting the same or different samples into the areas separated by the separating strips;

the third step: holding the sealing cover with the knob, and enabling the sealing cover to be fixed with the outer layer and seal the inner layer through the threaded rotation of the first threaded bulge and the second threaded bulge;

the fourth step: according to the anaerobic or aerobic condition of the liquid sample, the cover plate and the sealing cover can be separated or combined immovably, if the liquid sample is aerobic bacteria, the pinch plate is pulled, the alignment block is pulled out from the alignment groove, the cover plate is separated from the sealing cover, meanwhile, the sealing block is separated from the lantern ring, and air enters through the hole of the lantern ring.

Compared with the prior art, the invention has the beneficial effects that: the culture dish for bacterial culture and the use method are as follows: 1. through the mutual structure of the inner layer and the outer layer, the liquid sample can be protected doubly during culture, and even if the inner layer is not cracked, the outer layer can also be used for anti-leakage protection;

2. the separation strip can separate the space of the inner layer and realize the independent use of four areas, thereby being suitable for the culture of the same or a plurality of liquid samples and improving the practicability of the liquid samples;

3. through the hole of the lantern ring, the circulation of air can be realized to the inlayer, and the spill of liquid sample can be caused to the while also can prevent when shifting overall structure.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic top view of a separator strip according to the present invention;

FIG. 3 is a partially enlarged view of the connection between the elastic clip and the clip groove and the clip plate of the present invention;

fig. 4 is a schematic view of the seal block distribution of the present invention.

In the figure: 1 inner layer, 11 separating strips, 12 outer layers, 13 silica gel pads, 14 elastic clamps, 15 clamping grooves, 2 sealing covers, 21 first threaded bulges, 22 second threaded bulges, 3 cover plates, 31 knobs, 4 grooves, 41 springs, 42 sliding blocks, 43 buckling plates, 5 alignment blocks, 51 alignment grooves, 6 lantern rings and 61 sealing blocks.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, fig. 2 and fig. 3, the present invention provides a technical solution: the utility model provides a culture dish for bacterial culture, including inlayer 1, be provided with parting strip 11 in the inlayer 1, parting strip 11 divide into four regions with inlayer 1, independently part, can cultivate the same race or multiple bacterium, increase the practicality of single culture dish, be provided with outer 12 on inlayer 1's the outer wall, outer 12's lower extreme is provided with silica gel pad 13, outer 12 can play a protection effect to inlayer 1, silica gel pad 13 bonds through compound glue with outer 12, silica gel pad 13 can play the effect of a buffering protection, the both sides of inlayer 1 are provided with elasticity card 14, outer 12's both sides are provided with draw-in

groove

15, 14 joint draw-in grooves 15 of elasticity card, major structure such as inlayer 1 and outer 12 are polyethylene material and make.

Referring to fig. 1 and 3, the inner layer 1 is covered with the sealing cover 2, the inner wall of the sealing cover 2 is provided with an annular first thread protrusion 21, the outer layer 12 is provided with an annular second thread protrusion 22, and the first thread protrusion 21 is in threaded connection with the second thread protrusion 22, so that the inner layer 1 is conveniently sealed.

Referring to fig. 1, a cover plate 3 is disposed on a sealing cover 2, and a knob 31 is disposed on the cover plate 3 to facilitate opening of the sealing cover 2.

Referring to fig. 3, grooves 4 are formed in two sides of the cover plate 3, springs 41 are fixedly connected in the grooves 4, sliders 42 are fixedly connected to the other ends of the springs 41, buckling plates 43 are fixedly connected to the other ends of the sliders 42, aligning blocks 5 are fixedly connected to the buckling plates 43, aligning grooves 51 are formed in two sides of the sealing cover 2, the aligning blocks 5 are in sliding sleeve connection with the aligning grooves 51, and the cover plate 3 and the sealing cover 2 can be combined or separated conveniently.

Referring to fig. 1 and 4, the sealing cover 2 is embedded with a lantern ring 6, the cover plate 3 is fixedly connected with a sealing block 61, and the sealing block 61 is slidably sleeved with the lantern ring 6, so that air can be blocked or circulated according to anaerobic or aerobic conditions of bacteria culture.

Referring to fig. 1 and 2, the separation strip 11 has a cross structure, the lower end of the separation strip is fixedly adhered to the bottom surface of the inner layer 1, and the upper end of the separation strip is tightly attached to the sealing cover 2, so that the separation effect of the independent space is ensured.

Referring to fig. 1 and 3, the elastic card 14 is made of a regular hexagonal rubber material, and the shape of the card slot 15 is the same as that of the elastic card 14, and the size of the card slot is matched, so that the card is convenient to detach.

The use method of the culture dish for bacterial culture comprises the following steps:

the first step is as follows: firstly, the inner layer 1 and the outer layer 12 are combined and clamped into the clamping groove 15 through the elastic clamp 14, so that the two layers are fixed;

the second step is that: respectively putting the same or different samples into the areas separated by the separating strips 11;

the third step: holding the sealing cover 2 with the knob 31, and enabling the sealing cover 2 to be fixed with the outer layer 12 and sealing the inner layer 1 through the threaded rotation of the first threaded bulge 21 and the second threaded bulge 22;

the fourth step: according to the anaerobic or aerobic condition of the liquid sample, the cover plate 3 and the sealing cover 2 can be separated or combined immovably, if the liquid sample is aerobic bacteria, the pinch plate 43 is pulled, the alignment block 5 is pulled out from the alignment groove 51, so that the cover plate 3 is separated from the sealing cover 2, meanwhile, the sealing block 61 is separated from the lantern ring 6, and air enters through the holes of the lantern ring 6.

The invention is implemented as follows: when the device is used, the structures of all the parts are disinfected and dried, the inner layer 1 is clamped in the clamping groove 15 through the elastic clamp 14 and is combined and fixed with the outer layer 12, the inner layer and the outer layer are double-layer, the device is more durable than a single layer, the inner layer 1 cracks timely, the outer layer 12 can also play a role in assisting anti-seepage, then the knob 31 is rotated, the first threaded bulge 21 is in threaded connection with the second threaded bulge 22, the sealing cover 2 can be fixed with the outer layer 12, the inner layer 1 is sealed and covered, meanwhile, the sealing cover 2 is tightly attached to the upper end of the partition strip 11, the partition strip 11 can thoroughly separate the space of four areas, when a liquid sample is aerobic bacteria, in order to prevent the liquid from spilling caused by moving a culture dish, the buckle 43 is pulled outwards, and the alignment block 5 can slide out from the inner side of the alignment groove 51 through the stretching effect of the spring 41, therefore, the cover plate 3 can be separated from the sealing cover 2, the sealing block 61 slides out of the lantern ring 6, air circulation is achieved through the holes in the lantern ring 6, and sufficient oxygen is provided for aerobic bacteria.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A method for using a culture dish for bacterial culture is characterized in that: the culture dish for bacterial culture comprises an inner layer (1), wherein a separation strip (11) is arranged in the inner layer (1), an outer layer (12) is arranged on the outer wall of the inner layer (1), a silica gel pad (13) is arranged at the lower end of the outer layer (12), elastic cards (14) are arranged on two sides of the inner layer (1), clamping grooves (15) are arranged on two sides of the outer layer (12), and the elastic cards (14) are clamped with the clamping grooves (15);

the inner layer (1) is covered with a sealing cover (2), the inner wall of the sealing cover (2) is provided with an annular first threaded bulge (21), the outer layer (12) is provided with an annular second threaded bulge (22), and the first threaded bulge (21) is in threaded connection with the second threaded bulge (22);

a cover plate (3) is arranged on the sealing cover (2), and a knob (31) is arranged on the cover plate (3);

grooves (4) are formed in two sides of the cover plate (3), a spring (41) is fixedly connected in each groove (4), a sliding block (42) is fixedly connected to the other end of each spring (41), a buckling plate (43) is fixedly connected to the other end of each sliding block (42), a positioning block (5) is fixedly connected to each buckling plate (43), positioning grooves (51) are formed in two sides of the sealing cover (2), and the positioning blocks (5) are in sliding sleeve connection with the positioning grooves (51);

a lantern ring (6) is embedded in the sealing cover (2), a sealing block (61) is fixedly connected to the cover plate (3), and the sealing block (61) is sleeved with the lantern ring (6) in a sliding mode;

the first step is as follows: the inner layer (1) and the outer layer (12) are combined and clamped into the clamping groove (15) through the elastic clamp (14) so that the inner layer and the outer layer are fixed;

the second step is that: respectively putting the same or different samples into the areas separated by the separating strips (11);

the third step: holding the sealing cover (2) with the knob (31), and enabling the sealing cover (2) to be fixed with the outer layer (12) and seal the inner layer (1) through the threaded rotation of the first threaded bulge (21) and the second threaded bulge (22);

the fourth step: according to the anaerobic or aerobic condition of the liquid sample, the cover plate (3) and the sealing cover (2) can be separated or combined immovably, if the liquid sample is aerobic bacteria, the buckling plate (43) is pulled, the alignment block (5) is pulled out from the alignment groove (51), so that the cover plate (3) is separated from the sealing cover (2), meanwhile, the sealing block (61) is separated from the lantern ring (6), and air enters through the holes of the lantern ring (6).

2. The method of claim 1, wherein: the separation strip (11) is of a cross structure, the lower end of the separation strip is fixedly adhered to the bottom surface in the inner layer (1), and the upper end of the separation strip is tightly attached to the sealing cover (2).

3. The method of claim 1, wherein: the elastic card (14) is made of a regular hexagonal rubber material, and the clamping groove (15) is identical to the elastic card (14) in shape and matched in size.