CN109679828A - A kind of Bacteria Culture culture dish and application method - Google Patents
A kind of Bacteria Culture culture dish and application method Download PDFInfo
- Publication number
- CN109679828A CN109679828A CN201910134134.5A CN201910134134A CN109679828A CN 109679828 A CN109679828 A CN 109679828A CN 201910134134 A CN201910134134 A CN 201910134134A CN 109679828 A CN109679828 A CN 109679828A
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- internal layer
- culture dish
- sealing cover
- outer layer
- culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Abstract
The invention discloses a kind of Bacteria Culture culture dish and application method, including internal layer, divider is provided in internal layer, outer layer is provided on the outer wall of internal layer, the lower end of outer layer is provided with silicagel pad, and the two sides of internal layer are provided with elastic card, the two sides of outer layer are provided with card slot, and elastic card is clamped card slot.The Bacteria Culture culture dish and application method; pass through the mutual structure of internal layer and outer layer; so that fluid sample is in culture; dual protection can be obtained, even if internal layer cracking is not found, leakage protection can also be carried out by outer layer; divider can be separated the space of internal layer; and realize being independently operated, so as to be suitble to of the same race or plurality of liquid sample culture of four regions, improve the practicality.
Description
Technical field
The present invention relates to Bacteria Culture technical field, specially a kind of Bacteria Culture culture dish and application method.
Background technique
Culture dish is a kind of for microorganism or the labware of cell culture, predominantly plastic and glass, glass
The adhere-wall culture that can be used for vegetable material, microculture and zooblast；Plastics are mostly polythene materials, there is one
It is secondary property and nonexpondable, it is suitble to laboratory inoculation, scribing line, the operation of separation of bacterial, can be used for the culture of vegetable material.
Existing fluid sample culture dish is all substantially a disk and a sealing cover composition, when in use, if going out
Existing collision situation is easy to appear the case where cracking leaks, while the culture of some aerobic bacterias cannot use sealing cover, shift
When, it is easy to spill, brings unnecessary trouble to staff.
Summary of the invention
The purpose of the present invention is to provide a kind of Bacteria Culture culture dish and application method, with solve conventional culture dish to
Staff brings the problem of work inconvenience.
To achieve the above object, the invention provides the following technical scheme: a kind of Bacteria Culture culture dish, including internal layer,
It is provided with divider in the internal layer, outer layer is provided on the outer wall of the internal layer, the lower end of the outer layer is provided with silicagel pad,
The two sides of the internal layer are provided with elastic card, and the two sides of the outer layer are provided with card slot, and the elastic card is clamped card slot.
Preferably, it is covered with sealing cover on the internal layer, cricoid first thread protrusion is provided on the inner wall of sealing cover,
Cricoid second thread protrusion is provided on the outer layer, the first thread protrusion is threadedly coupled the second thread protrusion.
Preferably, it is provided with cover board on the sealing cover, is provided with knob on cover board.
Preferably, the two sides setting of the cover board is fluted, spring is fixedly connected in groove, the other end of spring is fixed
It is connected with sliding block, the other end of sliding block is fixedly connected with buckle, and bit block, the two sides of the sealing cover are fixedly connected on buckle
Alignment grooves are provided with, bit block is slidably socketed alignment grooves.
Preferably, it is embedded with lantern ring on the sealing cover, sealing block, sealing block sliding sleeve are fixedly connected on the cover board
Female connector ring.
Preferably, the divider is criss-cross construction, the bottom surface fixed bonding in lower end and internal layer, the upper end with
Sealing cover is close to.
Preferably, the elastic card is made of the rubber material of regular hexagon, the shape of the card slot and the shape of elastic card
Shape is identical, and size matches.
Preferably, the application method of the Bacteria Culture culture dish is as follows:
Step 1: first merging internal layer with outer layer, it is caught in card slot by elastic card, so that the two is fixed；
Step 2: of the same race or different types of sample is respectively put into the region of divider separation；
Step 3: the sealing cover with knob is held, by the first thread protrusion and the second thread protrusion threads turn, so that close
Capping can be fixed with outer layer, and be sealed to internal layer；
Step 4: according to the anaerobism of fluid sample or aerobic situation, cover board can be separated with sealing cover or merge it is motionless, if
Aerobic bacteria then pulls buckle, and bit block is pulled out out of alignment grooves, so that cover board and sealing cover disengage, meanwhile, sealing block and set
Ring separation, by the hole of lantern ring, realizes the entrance of air.
Compared with prior art, the beneficial effects of the present invention are: the Bacteria Culture culture dish and application method: 1, passing through
The mutual structure of internal layer and outer layer, so that fluid sample can obtain dual protection in culture, even if internal layer cracking is not sent out
It is existing, leakage protection can also be carried out by outer layer；
2, divider can be separated the space of internal layer, and realize that being independently operated, for four regions is same so as to be suitble to
The culture of kind or plurality of liquid sample improves the practicality；
3, by the hole of lantern ring, internal layer is enabled to realize the circulation of air, while be also possible to prevent in transfer overall structure
When, cause spilling for fluid sample.
Detailed description of the invention
Fig. 1 is overall structure diagram of the invention；
Fig. 2 is divider plan view shape schematic diagram of the invention；
Fig. 3 connects for elastic card of the invention with card slot and buckle part partial enlargement diagram；
Fig. 4 is sealing block distribution schematic diagram of the invention.
In figure: 1 internal layer, 11 dividers, 12 outer layers, 13 silicagel pads, 14 elastic cards, 15 card slots, 2 sealing covers, 21 first spiral shells
Line protrusion, 22 second thread protrusions, 3 cover boards, 31 knobs, 4 grooves, 41 springs, 42 sliding blocks, 43 buckles, 5 bit blocks, 51 contrapositions
Slot, 6 lantern rings, 61 sealing blocks.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 1, Fig. 2 and Fig. 3 are please referred to, the present invention provides a kind of technical solution: a kind of Bacteria Culture culture dish, including it is interior
Layer 1, is provided with divider 11 in internal layer 1, and divider 11 divides internal layer 1 for four regions, independent to separate, and can cultivate of the same race or more
Kind bacterium, increases the practicability of single culture dish, outer layer 12 is provided on the outer wall of internal layer 1, the lower end of outer layer 12 is provided with silicon
Rubber mat 13, outer layer 12 can play a protecting effect to internal layer 1, and silicagel pad 13 and outer layer 12 pass through compound glue sticking, silica gel
Pad 13 can play the effect of a buffer protection, and the two sides of internal layer 1 are provided with elastic card 14, and the two sides of outer layer 12 are provided with card
Slot 15, elastic card 14 are clamped card slot 15, and the main structures such as internal layer 1 and outer layer 12 are that polythene material is made.
Refering to fig. 1 and Fig. 3, it is covered with sealing cover 2 on internal layer 1, cricoid first screw thread is provided on the inner wall of sealing cover 2
Protrusion 21, cricoid second thread protrusion 22 is provided on outer layer 12, and the first thread protrusion 21 is threadedly coupled the second thread protrusion
22, convenient for being sealed to internal layer 1.
Refering to fig. 1, it is provided with cover board 3 on sealing cover 2, knob 31 is provided on cover board 3, is easy to open sealing cover 2.
Refering to Fig. 3, the two sides of cover board 3 are arranged fluted 4, are fixedly connected with spring 41, the other end of spring 41 in groove 4
It is fixedly connected with sliding block 42, the other end of sliding block 42 is fixedly connected with buckle 43, and bit block 5 is fixedly connected on buckle 43, close
The two sides of capping 2 are provided with alignment grooves 51, and bit block 5 is slidably socketed alignment grooves 51, convenient for merging to cover board 3 and sealing cover 2
Or it separates.
Refering to fig. 1 and Fig. 4, it is embedded with lantern ring 6 on sealing cover 2, sealing block 61, sealing block 61 is fixedly connected on cover board 3
It is slidably socketed lantern ring 6, blocking or circulating for air can be carried out according to the anaerobism or aerobic situation of Bacteria Culture.
Refering to fig. 1 and Fig. 2, divider 11 are criss-cross construction, the bottom surface fixed bonding in lower end and internal layer 1,
Upper end is close to sealing cover 2, guarantees the segregation of separate space.
It is made refering to fig. 1 with Fig. 3, elastic card 14 of the rubber material of regular hexagon, the shape and elastic card 14 of card slot 15
Shape it is identical, and size matches, clamping easy to disassemble.
The application method of the Bacteria Culture culture dish is as follows:
Step 1: first merging internal layer 1 with outer layer 12, it is caught in card slot 15 by elastic card 14, so that the two is fixed；
Step 2: of the same race or different types of sample is respectively put into the region that divider 11 separates；
Step 3: holding the sealing cover 2 with knob 31, turned by the first thread protrusion 21 and 22 screw thread of the second thread protrusion
It is dynamic, enable sealing cover 2 fixed with outer layer 12, and be sealed to internal layer 1；
Step 4: according to the anaerobism of fluid sample or aerobic situation, cover board 3 can be separated with sealing cover 2 or merge it is motionless, if
For aerobic bacteria, then buckle 43 is pulled, bit block 5 is pulled out out of alignment grooves 51, so that cover board 3 and sealing cover 2 disengage, meanwhile,
Sealing block 61 is separated with lantern ring 6, by the hole of lantern ring 6, realizes the entrance of air.
The present invention is in the specific implementation: when it is desired to be used, first drying Each part disinfection, then leads to internal layer 1
It crosses elastic card 14 to be clamped in card slot 15, merges fixation with outer layer 12, inside and outside bilayer is more durable compared to single layer, interior in time
There is crack in layer 1, and outer layer 12 can also play the effect of auxiliary seepage proof leakage, and then turning knob 31, utilize the first screw thread
Protrusion 21 is threadedly coupled the second thread protrusion 22, enables sealing cover 2 fixed with outer layer 12, to seal to internal layer 1
Covering, while sealing cover 2 is close to the upper end of divider 11, divider 11 is carried out to the space in four regions thorough
Separation, when fluid sample be aerobic bacteria when, move the case where culture dish causes liquid to spill in order to prevent, pull out buckle
43, by the drawing effect of spring 41, bit block 5 is skidded off from the inside of alignment grooves 51, so that cover board 3 can
Disengaged with sealing cover 2 so that sealing block 61 is skidded off out of lantern ring 6, by the hole of lantern ring 6, realize the circulation of air into
Enter, provides enough oxygen for aerobic bacteria.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (8)
1. a kind of Bacteria Culture culture dish, including internal layer (1), it is characterised in that: be provided with divider in the internal layer (1)
(11), it is provided with outer layer (12) on the outer wall of the internal layer (1), the lower end of the outer layer (12) is provided with silicagel pad (13), institute
The two sides for stating internal layer (1) are provided with elastic card (14), and the two sides of the outer layer (12) are provided with card slot (15), the elastic card
(14) card slot (15) are clamped.
2. a kind of Bacteria Culture culture dish according to claim 1, it is characterised in that: be covered on the internal layer (1)
Sealing cover (2) is provided with cricoid first thread protrusion (21) on the inner wall of sealing cover (2), is provided on the outer layer (12)
Cricoid second thread protrusion (22), the first thread protrusion (21) are threadedly coupled the second thread protrusion (22).
3. a kind of Bacteria Culture culture dish according to claim 2, it is characterised in that: be arranged on the sealing cover (2)
Have cover board (3), knob (31) are provided on cover board (3).
4. a kind of Bacteria Culture culture dish according to claim 3, it is characterised in that: the two sides of the cover board (3) are set
It is equipped with groove (4), is fixedly connected with spring (41) in groove (4), the other end of spring (41) is fixedly connected with sliding block (42), sliding
The other end of block (42) is fixedly connected with buckle (43), is fixedly connected on buckle (43) bit block (5), the sealing cover (2)
Two sides be provided with alignment grooves (51), bit block (5) is slidably socketed alignment grooves (51).
5. a kind of Bacteria Culture culture dish according to claim 3, it is characterised in that: embedded on the sealing cover (2)
Have lantern ring (6), is fixedly connected with sealing block (61) on the cover board (3), sealing block (61) is slidably socketed lantern ring (6).
6. a kind of Bacteria Culture culture dish according to claim 1, it is characterised in that: the divider (11) is cross
Intersection construction, lower end and the bottom surface fixed bonding in internal layer (1), the upper end is close to sealing cover (2).
7. a kind of Bacteria Culture culture dish according to claim 1, it is characterised in that: elastic card (14) You Zhengliu
The rubber material of side shape is made, and the shape of the card slot (15) is identical as the shape of elastic card (14), and size matches.
8. a kind of Bacteria Culture culture dish according to claim 1, it is characterised in that: the Bacteria Culture culture dish
Application method is as follows:
Step 1: first merging internal layer (1) with outer layer (12), it is caught in card slot (15) by elastic card (14), so that the two is solid
It is fixed；
Step 2: of the same race or different types of sample is respectively put into the region of divider (11) separation；
Step 3: holding the sealing cover (2) with knob (31), pass through the first thread protrusion (21) and the second thread protrusion (22)
Threads turn so that sealing cover (2) can be fixed with outer layer (12), and is sealed internal layer (1)；
Step 4: cover board (3) can be separated with sealing cover (2) or be merged not according to the anaerobism of fluid sample or aerobic situation
It is dynamic, if aerobic bacteria, then buckle (43) are pulled, bit block (5) is pulled out out of alignment grooves (51), so that cover board (3) and sealing
(2) are covered to disengage, meanwhile, sealing block (61) is separated with lantern ring (6), by the hole of lantern ring (6), realizes the entrance of air.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910134134.5A CN109679828B (en) | 2019-02-22 | 2019-02-22 | Culture dish for bacterial culture and use method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910134134.5A CN109679828B (en) | 2019-02-22 | 2019-02-22 | Culture dish for bacterial culture and use method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109679828A true CN109679828A (en) | 2019-04-26 |
| CN109679828B CN109679828B (en) | 2020-06-09 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910134134.5A Active CN109679828B (en) | 2019-02-22 | 2019-02-22 | Culture dish for bacterial culture and use method |
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| Country | Link |
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| CN (1) | CN109679828B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112029642A (en) * | 2020-09-17 | 2020-12-04 | 张贺喜 | Device for extracting cells from stem of corydalis bungeana |
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| US20110003376A1 (en) * | 2009-03-09 | 2011-01-06 | Eppendorf Ag | Cell Culture Dish |
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| CN208151366U (en) * | 2018-03-29 | 2018-11-27 | 江西省科星生物工程有限公司 | Culture dish is used in a kind of experiment of biological medicine |
| CN208218852U (en) * | 2018-03-15 | 2018-12-11 | 成都康华生物制品股份有限公司 | A kind of Bacteria Culture culture dish |
| CN208485874U (en) * | 2018-07-11 | 2019-02-12 | 宁夏农林科学院农作物研究所（宁夏回族自治区农作物育种中心） | It is a kind of that ventilative culture dish is adjusted |
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2019
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|---|---|---|---|---|
| CN85106361A (en) * | 1984-10-09 | 1986-04-10 | 小林制药株式会社 | The method of training Ti Shi culture dish of culturing bacterium and inspection drug susceptibility |
| US20100093075A1 (en) * | 2007-05-24 | 2010-04-15 | Mueller Rolf | Receptacle for accepting nutrient media |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112029642A (en) * | 2020-09-17 | 2020-12-04 | 张贺喜 | Device for extracting cells from stem of corydalis bungeana |
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| Publication number | Publication date |
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| CN109679828B (en) | 2020-06-09 |
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Effective date of registration: 20200415 Address after: 238000 Anhui Chaohu Economic Development Zone Laoshan South Road Hefei vocational and Technical College Applicant after: Xu Zhenguo Address before: 238000 Anhui Hefei Chaohu Anhui Chaohu Economic Development Zone Hefei Career Technical College Applicant before: HEFEI TECHNOLOGY College |
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Effective date of registration: 20211019 Address after: 238000 Hefei vocational and technical college, Laoshan South Road, Chaohu Economic Development Zone, Hefei City, Anhui Province Patentee after: HEFEI TECHNOLOGY College Address before: 238000 Hefei vocational and technical college, Laoshan South Road, Chaohu Economic Development Zone, Anhui Province Patentee before: Xu Zhenguo |