CN205115494U - Cell culture bottle - Google Patents
Cell culture bottle Download PDFInfo
- Publication number
- CN205115494U CN205115494U CN201520890647.6U CN201520890647U CN205115494U CN 205115494 U CN205115494 U CN 205115494U CN 201520890647 U CN201520890647 U CN 201520890647U CN 205115494 U CN205115494 U CN 205115494U
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- China
- Prior art keywords
- bottle
- connecting hole
- lid
- sealing cover
- air filter
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Abstract
The utility model discloses a cell culture bottle, including training the bottle and rather than screw thread fit connection's bottle lid, the bottle lid is including connecting the connection lid of training the bottle, it has the connecting hole to connect to cover to open, from the top down is equipped with sealed lid and air filter membrane rather than interference fit in proper order in the connecting hole, sealed covering there is the air vent along its circumference equipartition. The utility model discloses set up the air vent on the bottle lid, simultaneously the below of the air vent on bottle lid installation air filter membrane, need not open under the condition of bottle lid, realize the internal outer gaseous exchange of blake bottle through the air vent, adopt the air filter membrane to filter getting into the internal air of blake bottle simultaneously, prevent that the dust of the external portion of blake bottle and bacterium from entering into the internal portion's pollution culture solution of blake bottle, for further reinforcing filter capacity, be split type with the bottle lid design, including connecting the lid and sealing up the lid, install the air filter membrane respectively in sealed lid bottom with in connecting the lid, realize the double -deck filtration to the air.
Description
Technical field
The utility model relates to microorganism culturing container technical field, particularly relates to a kind of Tissue Culture Flask.
Background technology
The vitro culture of biological cells and tissues has become to one of important topic of life studies and practice.Cultured cells is of a great variety, as virus, bacterium, fungi, human body cell and animal and plant cells.In medicine and pharmacology and biological study, usually select culturing bottle as the container of cell cultures.
Culturing bottle is generally made up of bottle and bottle cap, because culturing cell needs ventilative, therefore needs, while realization ventilation, to prevent the dust of bottle outside and bacterium from entering into bottle inside, affect the accuracy of culturing cell test-results.Mostly existing Tissue Culture Flask is that the method adopting cotton bottle stopper or directly unscrew bottle cap is to strengthen the gaseous interchange inside and outside bottle, then very easily causes nutrient solution to pollute like this, and is unfavorable for the gaseous interchange inside and outside bottle.
Utility model content
The purpose of this utility model is to provide a kind of Tissue Culture Flask, is realizing preventing the dust of bottle outside and bacterium from entering into bottle inside so that polluting nutrient solution inside and outside culturing bottle while gaseous interchange.
For reaching this object, the utility model by the following technical solutions:
A kind of Tissue Culture Flask, comprise the bottle cap cultivated bottle and be connected with its thread fit, described bottle cap comprises the connection cover connecting and cultivate bottle, described connection cover has connecting hole, is provided with successively from top to bottom and the sealing cover of its shrink-fit and air filter film in described connecting hole; Described sealing cover is evenly equipped with ventilating pit along its circumference.
As preferably, described connecting hole is shoulder hole, and it comprises the first connecting hole, the second connecting hole, the 3rd connecting hole and the 4th connecting hole that distribute successively from top to bottom.
As preferably, described first connecting hole and the 4th connecting hole are threaded hole, and described connection cover is connected by thread fit with sealing cover by the first connecting hole, described bottle cap by the 4th connecting hole with cultivate bottle thread fit and be connected.
As preferably, the aperture of described first connecting hole, the second connecting hole and the 3rd connecting hole reduces successively, and described air filter film is positioned on the step surface that the second connecting hole and the 3rd connecting hole formed.
As preferably, be provided with the gland suitable with the second connecting hole between described air filter film and sealing cover, described gland is provided with through hole.
As preferably, described ventilating pit is greater than the through hole radius on described gland near the distance between the inwall and sealing cover central axis of sealing cover central axis.
As preferably, described ventilating pit is less than or equal to the through hole radius on described gland near the distance between the inwall and sealing cover central axis of sealing cover central axis.
As preferably, the bottom of described sealing cover is provided with air filter film.
As preferably, described gland thickness and the thickness sum of air filter film being positioned at gland bottom equal the hole depth of the 3rd connecting hole.
As preferably, described ventilating pit is provided with 8,12 or 16.
The beneficial effects of the utility model: the utility model offers ventilating pit on bottle cap, install air filter film simultaneously below bottle cap upper vent hole; When not needing to open bottle cap, realized the gaseous interchange of culturing bottle inside and outside by ventilating pit; Adopting air filter film to filter entering the air cultivated in bottle simultaneously, preventing the dust of culturing bottle external body and bacterium from entering into and cultivating bottle internal contamination nutrient solution; In order to further strengthen filtration capacity, bottle cap being designed to split type, comprising connection cover and sealing cover, bottom sealing cover He in connection cover, respectively air filter film is installed, realize the double medium filtration to air; The gland of pressuring action is set above lower air filter membrane, by changing the position relationship of the through-hole diameter on gland and the ventilating pit on sealing cover or sealing cover relative to the position of gland, ventilation demand can have been met or sealed demand completely in addition.
Accompanying drawing explanation
Fig. 1 is the structural representation of Tissue Culture Flask described in the utility model;
Fig. 2 is the structural representation of bottle cap described in embodiment one;
Fig. 3 is the structural representation of connection cover described in the utility model;
Fig. 4 is the structural representation of sealing cover described in the utility model;
Fig. 5 is the aeration status figure of Tissue Culture Flask described in embodiment one;
Fig. 6 is the structural representation of Tissue Culture Flask described in embodiment two.
In figure:
1, bottle is cultivated; 2, bottle cap; 21, connection cover; 211, connecting hole; 212, the first connecting hole; 213, the second connecting hole; 214, the 3rd connecting hole; 215, the 4th connecting hole; 22, sealing cover; 221, ventilating pit; 23, air filter film; 24, gland.
Embodiment
The technical solution of the utility model is further illustrated by embodiment below in conjunction with accompanying drawing.
Embodiment one
Fig. 1 is the structural representation of Tissue Culture Flask described in the present embodiment.As shown in Figure 1, described Tissue Culture Flask comprises the bottle cap 2 cultivated bottle 1 and be connected with its thread fit, described bottle cap 2 comprises the connection cover 21 connecting and cultivate bottle 1, described connection cover 21 has connecting hole 211, is provided with successively from top to bottom in described connecting hole 211 and the sealing cover 22 of its shrink-fit and air filter film 23; Described sealing cover 22 is evenly equipped with ventilating pit 221 along its circumference, and the air cultivating bottle 1 outside is entered in bottle cap 2 by ventilating pit 221, and then enters in culturing bottle body 1 after air filter film 23 filters.When not needing to open bottle cap, realize the gaseous interchange inside and outside culturing bottle by ventilating pit 221; Adopting air filter film 23 to filter entering the air cultivated in bottle 1 simultaneously, preventing the dust of cultivation bottle 1 outside and bacterium from entering into and cultivating bottle 1 internal contamination nutrient solution.
In the present embodiment, as shown in Figure 2, described connecting hole 211 is shoulder hole, and it comprises the first connecting hole 212, second connecting hole 213, the 3rd connecting hole 214 and the 4th connecting hole 215 that distribute successively from top to bottom.Described first connecting hole 212 and the 4th connecting hole 215 are threaded hole, and described connection cover 21 is connected by thread fit with sealing cover 22 by the first connecting hole 212, and described bottle cap 1 is connected with cultivation bottle 1 thread fit by the 4th connecting hole 215.
In the present embodiment, the aperture of described first connecting hole 212, second connecting hole 213 and the 3rd connecting hole 214 reduces successively, described air filter film 23 is positioned on the step surface of the second connecting hole 213 and the formation of the 3rd connecting hole 214, is supported air filter film 23 by this step surface.
In the present embodiment, be provided with the gland 24 suitable with the second connecting hole 213 between described air filter film 23 and sealing cover 22, compress air filter film 23 by gland 24, then screwing hermetic lid 22, make the lower surface of sealing cover 22 be pressed onto on gland 24; Described gland 24 is provided with through hole, enters the air in bottle cap 2 by ventilating pit 221, and in overpressure cap 24, through hole flows to air filter film 23.
As preferably, described ventilating pit 221 is greater than the through hole radius on described gland 24 near the distance between the inwall and sealing cover 22 central axis of sealing cover 22 central axis, this Tissue Culture Flask can realize sealing completely and can ventilating.Concrete, as shown in Figure 1, screwing hermetic lid 22 makes its lower surface be pressed onto on gland 24, and ventilating pit 221 is glanded 24 completely and blocks, and now Tissue Culture Flask seals completely; When needs are ventilated, as shown in Figure 4, unscrew sealing cover 22 and make its segment distance that moves up, now enter the air in bottle cap 2 from ventilating pit 221, the through hole can be passed through on gland 24 flows to air filter film.
In order to further improve air filtration effect, the present embodiment establishes air filter film 23 in the bottom of described sealing cover 22, realizes double medium filtration.
Described gland 24 thickness equals the degree of depth of the 3rd connecting hole 214 with the thickness sum of the air filter film 23 being positioned at gland 24 bottom, so that be pressed onto on sealing cover 24 upper surface when sealing cover 22 is threaded to bottom, improves sealing effectiveness.
Ventilating pit 221 described in the present embodiment is provided with 8,12 or 16.As shown in Figure 3, the present embodiment is preferably set to 16.
Embodiment two
In the present embodiment, as shown in Figure 5, and the difference of embodiment one be: ventilating pit 221 described in the present embodiment is less than or equal to the through hole radius on described gland 24 near the distance between the inwall and sealing cover 22 central axis of sealing cover 22 central axis.Now gland 24 does not block ventilating pit 221 completely, therefore under state sealing cover 22 not adjusted, make culturing bottle described in the present embodiment be in aeration status always, avoid and repeatedly screw sealing cover 22 sealing property between sealing cover 22 and connection cover 21 is deteriorated.
Obviously, above-described embodiment of the present utility model is only used to clearly demonstrate the utility model example, and is not the restriction to embodiment of the present utility model.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.All do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., within the protection domain that all should be included in the utility model claim.
Claims (10)
1. a Tissue Culture Flask, comprise the bottle cap (2) cultivated bottle (1) and be connected with its thread fit, it is characterized in that, described bottle cap (2) comprises the connection cover (21) connecting and cultivate bottle (1), described connection cover (21) has connecting hole (211), be provided with successively from top to bottom in described connecting hole (211) and the sealing cover of its shrink-fit (22) and air filter film (23); Described sealing cover (22) is evenly equipped with ventilating pit (221) along its circumference.
2. Tissue Culture Flask according to claim 1, it is characterized in that, described connecting hole (211) is shoulder hole, and it comprises the first connecting hole (212), the second connecting hole (213), the 3rd connecting hole (214) and the 4th connecting hole (215) that distribute successively from top to bottom.
3. Tissue Culture Flask according to claim 2, it is characterized in that, described first connecting hole (212) and the 4th connecting hole (215) are threaded hole, described connection cover (21) is connected by thread fit with sealing cover (22) by the first connecting hole (212), and described bottle cap (2) is connected with cultivation bottle (1) thread fit by the 4th connecting hole (215).
4. Tissue Culture Flask according to claim 3, it is characterized in that, the aperture of described first connecting hole (212), the second connecting hole (213) and the 3rd connecting hole (214) reduces successively, and described air filter film (23) is positioned on the step surface that the second connecting hole (212) and the 3rd connecting hole (214) formed.
5. Tissue Culture Flask according to claim 4, it is characterized in that, be provided with the gland (24) suitable with the second connecting hole (213) between described air filter film (23) and sealing cover (22), described gland (24) is provided with through hole.
6. Tissue Culture Flask according to claim 5, it is characterized in that, described ventilating pit (221) is greater than the through hole radius in institute's gland (24) near the distance between the inwall and sealing cover (22) central axis of sealing cover (22) central axis.
7. Tissue Culture Flask according to claim 5, it is characterized in that, described ventilating pit (221) is less than or equal to the through hole radius in institute's gland (24) near the distance between the inwall and sealing cover (22) central axis of sealing cover (22) central axis.
8. Tissue Culture Flask according to claim 5, is characterized in that, the bottom of described sealing cover (22) is provided with air filter film (23).
9. Tissue Culture Flask according to claim 5, it is characterized in that, described gland (24) thickness equals the hole depth of the 3rd connecting hole (214) with the thickness sum of the air filter film (23) being positioned at gland (24) bottom.
10. Tissue Culture Flask according to claim 1, is characterized in that, described ventilating pit (221) is provided with 8,12 or 16.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520890647.6U CN205115494U (en) | 2015-11-10 | 2015-11-10 | Cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520890647.6U CN205115494U (en) | 2015-11-10 | 2015-11-10 | Cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205115494U true CN205115494U (en) | 2016-03-30 |
Family
ID=55571117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520890647.6U Active CN205115494U (en) | 2015-11-10 | 2015-11-10 | Cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205115494U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365697A (en) * | 2017-08-08 | 2017-11-21 | 广西柳城县绿之缘生态农业科技有限公司 | The preparing device of soil probiotics |
| CN109913366A (en) * | 2019-05-08 | 2019-06-21 | 青海省畜牧兽医科学院 | A kind of culture bottle and the culture apparatus of filamentous fungi |
-
2015
- 2015-11-10 CN CN201520890647.6U patent/CN205115494U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365697A (en) * | 2017-08-08 | 2017-11-21 | 广西柳城县绿之缘生态农业科技有限公司 | The preparing device of soil probiotics |
| CN109913366A (en) * | 2019-05-08 | 2019-06-21 | 青海省畜牧兽医科学院 | A kind of culture bottle and the culture apparatus of filamentous fungi |
| CN109913366B (en) * | 2019-05-08 | 2024-03-22 | 青海省畜牧兽医科学院 | Culture bottle and filamentous fungus's culture apparatus |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |