CN207159214U - A kind of Tissue Culture Plate - Google Patents
A kind of Tissue Culture Plate Download PDFInfo
- Publication number
- CN207159214U CN207159214U CN201721171129.4U CN201721171129U CN207159214U CN 207159214 U CN207159214 U CN 207159214U CN 201721171129 U CN201721171129 U CN 201721171129U CN 207159214 U CN207159214 U CN 207159214U
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- culture
- hole
- culture plate
- exchange
- independent
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Abstract
The utility model discloses a kind of Tissue Culture Plate, including culture plate, disk cover, and middle spacer sleeve is provided between the culture plate, disk cover, and the wall with through hole is provided with middle spacer sleeve, NF membrane is provided with above wall；Center exchanging area, intermediate buffer and peripheral independent zones that large baffle separates are provided with culture plate, exchanging area is divided into multiple exchange culture units by large baffle, each exchange culture unit includes the independent culture hole of exchange culture hole placed in the middle and surrounding, separated between the exchange culture hole and independent culture hole by demarcation strip, demarcation strip is provided with exchange groove.The utility model Tissue Culture Plate is simply applicable, the exchanging area that can be communicated with each other and act on by the cellular informatics set up between each culture hole, and as control intermediate buffer, peripheral independent zones, it can be used in the different intercellular experiments that influence each other of each culture hole, comparative study can be carried out, it is easy to experimental observation and contrast, it is easy to use.
Description
Technical field
Bioexperiment clothing arts are the utility model is related to, specially a kind of Tissue Culture Plate.
Background technology
Culture plate is used to cultivating microorganism (bacterium and fungi) and cell in the laboratory of biology or medical science and (such as fed
Newborn zooblast, cell tissue etc.).As the container of culture medium, culture plate is in cell or field of microbial culture technology
There are many years using oneself, often use in the lab.Culture plate is typically square, has multiple cultures by receiving culture medium
The plate body in hole and the plate lid composition to match.Plate lid is positioned on plate body, and culture medium can be prevented by microorganism in external environment condition etc.
Pollution, while allow air to pass through in favor of the survival of cell or microorganism, growth and observation, detection.Make the material of culture plate
Material includes glass, synthetic resin, quartz and corrosion resistant metal (less use) etc..
Cell culture technology widely uses in medical science and biological study with application field.Generally, using Tissue Culture Plate
Contain fluid nutrient medium, cell adherent growth or suspension growth in a liquid.The hole count of the culture hole of conventional Tissue Culture Plate
There are 6,12,24,48,96,384,1536 holes etc..According to type, required volume of culture and the difference of culture cell when specifically chosen
Experiment purpose depending on.
But at present common Tissue Culture Plate have one it is common the characteristics of, i.e., definitely separate between each culture hole, no
Allow communicating with each other for cellular informatics between different culture holes, so also being done different intercellular influence each other each culture hole
Not comparative study.
Utility model content
The purpose of this utility model be to provide it is a kind of it is simple be applicable, can be used in the different intercellular phases of each culture hole
Experiment is mutually influenceed, carries out the Tissue Culture Plate of comparative study.
The purpose of this utility model can be achieved through the following technical solutions：
A kind of Tissue Culture Plate, including culture plate, disk cover, the culture plate, disk cover are square, the culture plate, disk
Middle spacer sleeve is provided between lid, the middle spacer sleeve is the square tubulose of both ends open, between being provided with the middle spacer sleeve
Interlayer, the wall are provided with the through hole of dense distribution, are provided with NF membrane above the wall, in the disk cover side wall
It is provided with multiple air-vents；Three regions are provided with the culture plate, be respectively by large baffle separate in most
The independent zones of the exchanging area of the heart, middle buffering area and outermost, the exchanging area is divided into multiple exchanges by large baffle
Unit is cultivated, each exchange culture unit includes exchange culture hole placed in the middle and multiple independent culture holes positioned at surrounding, institute
State and separated between exchange culture hole and independent culture hole by demarcation strip, the demarcation strip is provided with exchange groove.
Further, the buffering area is divided into multiple buffering culture subregions, each buffering culture by large baffle
Multiple buffering culture holes are separated into by small baffle in subregion；Multiple independent cultures are separated into by large baffle in the independent zones
Hole.
Further, the large baffle top is provided with sealing gasket, and raised item is provided with below the wall, described convex
It is corresponding with the large baffle in culture plate to play bar, the raised item top is provided with the soft bar of sealing.
The beneficial effects of the utility model：
The utility model Tissue Culture Plate is simply applicable, spacer sleeve in being set wherein between culture plate, disk cover, middle spacer sleeve
It is middle set with through hole wall, and wall above be provided with NF membrane, can keep culture plate inner air and outer air can
In the case of circulation, completely cut off outer aerial pollutant, reduce empirical risk；By large baffle, small baffle by culture plate
Numerous independent or quasi-sovereign culture region is separated into, meets the environment of the different intercellular experiments that influence each other of each culture hole
Demand；Exchanging area in culture plate, the independent culture of exchange culture hole placed in the middle and surrounding is separated into using demarcation strip
Hole, communicating with each other and acting on for the cellular informatics between each culture hole is promoted by the exchange groove set up on demarcation strip, is easy to try
Observation and contrast are tested, it is easy to use.
Brief description of the drawings
For the ease of it will be appreciated by those skilled in the art that being further described below in conjunction with the accompanying drawings to the utility model.
Fig. 1 is the example of the utility model structural front view；
Fig. 2 is the example of the utility model culture plate structure top view.
In figure：1st, culture plate, 11, exchanging area, 111, exchange culture hole, 112, independent culture hole, 12, buffering area, 121,
Buffer culture hole, 13, independent zones, 131, independent culture hole, 2, disk cover, 21, air-vent, 3, middle spacer sleeve, 31, wall,
311st, through hole, 312, raised item, 313, the soft bar of sealing, 32, NF membrane, 41, large baffle, 42, small baffle, 43, demarcation strip, 5, close
Packing.
Embodiment
Such as Fig. 1,2, a kind of Tissue Culture Plate, including culture plate 1, disk cover 2, the culture plate 1, disk cover 2 are square, institute
State and multiple air-vents 21 are provided with the side wall of disk cover 2.Middle spacer sleeve 3 is provided between the culture plate 1, disk cover 2, wherein among
Spacer 3 is the square tubulose of both ends open, wall 31 is provided with middle spacer sleeve 3, wall 31 is provided with dense distribution
Through hole 311, the top of wall 31 is provided with NF membrane 32.
Such as Fig. 1,2, three regions are provided with the culture plate 1, be respectively by large baffle 41 separate positioned at most
The independent zones 13 of the exchanging area 11 at center, middle buffering area 12 and outermost.The exchanging area 11 is divided by large baffle 41
Multiple exchange culture units are cut into, each exchange culture unit includes exchange culture hole 111 placed in the middle and positioned at the more of surrounding
Individual individually culture hole 112, wherein being separated between exchange culture hole 111 and individually culture hole 112 by demarcation strip 43, in demarcation strip
43 are provided with exchange groove, facilitate communicating with each other and acting on for each individually cellular informatics between culture hole 112, be easy to experimental observation and
Contrast；The buffering area 12 is divided into multiple buffering culture subregions by large baffle 41, and each buffering culture subregion passes through septulum
Plate 42 is separated into multiple buffering culture holes 121, and each buffering culture subregion is each independent, and each buffers air in culture subregion
Naturally circulate, be easy to that buffering culture subregion is each buffered the influencing each other for iuntercellular culture in culture hole 121 and observed；
Multiple independent culture holes 131 are separated into by large baffle 41 in the independent zones 13, each mutual not shadow of independent culture hole 131
Ring, paired observation experiment can be carried out as a control group.The top of large baffle 41 in the culture plate 1 is provided with sealing gasket 5, and
The lower section of wall 31 is provided with raised item 312, and raised item 312 is corresponding with the large baffle 41 in culture plate 1, and raised item 312
Top is provided with the soft bar 313 of sealing, and the sealing gasket 5 can be fixed together with sealing after soft bar 313 is in contact, and make culture plate 1
The section that interior large baffle 41 separates is separate, and mutual air will not circulate, and maintains independent environmental requirement.
Above content is only to the utility model structure example and explanation, the technology people of affiliated the art
Member is made various modifications or supplement to described instantiation or substituted using similar mode, without departing from practicality
New structure surmounts scope defined in the claims, all should belong to the protection domain of utility model.
Claims (3)
1. a kind of Tissue Culture Plate, including culture plate (1), disk cover (2), the culture plate (1), disk cover (2) are square, and it is special
Sign is, middle spacer sleeve (3) is provided between the culture plate (1), disk cover (2), and the middle spacer sleeve (3) is both ends open
Square tubulose, wall (31) is provided with the middle spacer sleeve (3), the wall (31) is provided with the through hole of dense distribution
(311) NF membrane (32), is provided with above the wall (31), multiple air-vents are provided with disk cover (2) side wall
(21)；Three regions are provided with the culture plate (1), are the friendship positioned at bosom separated by large baffle (41) respectively
The independent zones (13) of area (11), middle buffering area (12) and outermost are flowed, the exchanging area (11) passes through large baffle (41)
Multiple exchange culture units are divided into, each exchange culture unit includes exchange culture hole (111) placed in the middle and positioned at surrounding
It is multiple individually culture holes (112), it is described exchange culture hole (111) and independent culture hole (112) between pass through demarcation strip (43)
Separate, the demarcation strip (43) is provided with exchange groove.
2. a kind of Tissue Culture Plate according to claim 1, it is characterised in that the buffering area (12) passes through large baffle (41)
Multiple buffering culture subregions are divided into, being separated into multiple bufferings by small baffle (42) in each buffering culture subregion cultivates
Hole (121)；Multiple independent culture holes (131) are separated into by large baffle (41) in the independent zones (13).
3. a kind of Tissue Culture Plate according to claim 2, it is characterised in that large baffle (41) top is provided with sealing
Pad (5), be provided with raised item (312) below the wall (31), the raised item (312) with culture plate (1) it is big every
Plate (41) is corresponding, and raised item (312) top is provided with the soft bar (313) of sealing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721171129.4U CN207159214U (en) | 2017-09-13 | 2017-09-13 | A kind of Tissue Culture Plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721171129.4U CN207159214U (en) | 2017-09-13 | 2017-09-13 | A kind of Tissue Culture Plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207159214U true CN207159214U (en) | 2018-03-30 |
Family
ID=61721439
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721171129.4U Expired - Fee Related CN207159214U (en) | 2017-09-13 | 2017-09-13 | A kind of Tissue Culture Plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207159214U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112011452A (en) * | 2020-09-21 | 2020-12-01 | 新疆维吾尔自治区人民医院 | Drug sensitive detection plate |
-
2017
- 2017-09-13 CN CN201721171129.4U patent/CN207159214U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112011452A (en) * | 2020-09-21 | 2020-12-01 | 新疆维吾尔自治区人民医院 | Drug sensitive detection plate |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180330 Termination date: 20210913 |