CN108660066A - Preclinical medicine pathogen culture ware - Google Patents
Preclinical medicine pathogen culture ware Download PDFInfo
- Publication number
- CN108660066A CN108660066A CN201810509042.6A CN201810509042A CN108660066A CN 108660066 A CN108660066 A CN 108660066A CN 201810509042 A CN201810509042 A CN 201810509042A CN 108660066 A CN108660066 A CN 108660066A
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- CN
- China
- Prior art keywords
- ware
- groove
- ware body
- barrier
- barrier circle
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to medical experiment appliance technologies, more particularly to a kind of preclinical medicine pathogen culture ware, including transparent ware body and ware lid, bottom is connected with barrier circle in ware body, the first groove is offered along barrier circle upper surface, the restriction plate of multiple and different arc length is slidably connected in first groove, winding spacer ring lateral wall is provided with several shelves items for connecting side wall in barrier circle and ware body, space between barrier circle and ware body ware wall is separated into several cultivation regions by shelves item, it is both provided with mark and air hole on the external side wall of each corresponding ware in cultivation region, it is equipped with the second groove along ware lid edge, and ware body open edge be adapted with the second groove it is sliding.Multiple cultivation regions are arranged in the present invention, can cultivate multiple pathogens simultaneously and be convenient for comparative study, while exchanging area is arranged, communicate with each other convenient for information between pathogen cells between cultivation region, carry out cell-tocell transduction mechanism and signaling molecule screening study.
Description
Technical field
The invention belongs to medical experiment appliance technology, more particularly to a kind of preclinical medicine pathogen culture ware.
Background technology
Culture dish is a kind of labware being used for microorganism or cell culture, by the bottom and one of a flat circle plate-like
A lid composition, is generally made of glass or plastics.Culture dish material is essentially divided into two classes, predominantly plastic and glass, glass
The adhere-wall culture that can be used for vegetable material, microculture and zooblast of glass may also be used.Plastics may be poly-
Vinyl material, once property and it is nonexpondable, be suitble to laboratory inoculation, scribing line, separation of bacterial operation, can be used for
The culture of vegetable material.
Pathogen refers to microorganism, parasite or other media (the microorganism weight that people or animals and plants can be caused to catch
Group body includes hybrid or mutant), wherein microorganism accounts for the overwhelming majority, including virus, Chlamydia, Richettsia, mycoplasma,
Bacterium, conveyor screw and fungi；Parasite mainly has protozoon and Ru worms.It is generally required to cause of disease in the daily research of preclinical medicine
Body carries out in vitro culture, and the associated metabolic and growth characteristics of pathogen are understood with this.But it is used in existing preclinical medicine operation
Pathogen culture ware has the following disadvantages：：In culture dish can not subregion, it is very inconvenient when being pathogen contrast experiment；Because being
Circle, orientation have no idea to recognize, and operator can be drawn or be marked with marking pen behind in culture dish in many cases, this
If sample wipes out, experiment can only be just reformed.
Invention content
Multiple cultivation regions are arranged in the present invention, can cultivate multiple pathogens simultaneously and be convenient for comparative study, while exchange is arranged
Area communicates with each other convenient for information between pathogen cells between cultivation region, carries out cell-tocell transduction mechanism and signaling molecule sieve
Choosing research.
The present invention provides a kind of preclinical medicine pathogen culture ware, including transparent ware body and ware lid, the wares
Internal bottom is connected with barrier circle, is exchanging area in the barrier circle, and the first groove, institute are offered along barrier circle upper surface
The restriction plate for slidably connecting multiple and different arc length in the first groove is stated, is equipped with around barrier circle lateral wall several for connecting
The space between the ware body ware wall is enclosed in the barrier by the shelves item of side wall in the barrier circle and the ware body, the shelves item
Several cultivation regions are separated into, and the height of the shelves item is higher than the height of the barrier circle, the restriction plate and the shelves item
It is highly identical, it is arranged at intervals with vertical scale on the shelves item, each on the corresponding external side wall of ware in the cultivation region
Be both provided with mark and air hole, and on the external side wall of the ware, the air hole edge be provided with removable cover, the activity
Lid, which can cover, closes the air hole；
It is equipped with the second groove along the ware lid edge, and the ware body open edge is adapted cunning with second groove
It connects, in the ware lid, is provided with rubber pad in the second groove enclosing region, and the rubber pad can contact institute respectively
State restriction plate and the shelves item.
Preferably, ware body bottom is connected with stabilizer blade, and is arranged with rubber sleeve outside the stabilizer blade.
Preferably, it is provided with filter membrane in the air hole, and sterilization adsorbent is distributed on the filter membrane.
Preferably, the sterilization adsorbent is the active powdered carbon for being impregnated with thimerosal.
Preferably, the quantity of the shelves item is 4, and is uniformly distributed around barrier circle, the radian of the restriction plate
1/4,1/2 or the 3/4 of a length of barrier circle periphery perimeter.
Compared with prior art, beneficial effects of the present invention：
Multiple cultivation regions mutually completely cut off are arranged in the present invention, avoid interfering between pathogen, while can cultivate a variety of diseases
Substance is convenient for comparative study；The pathogen information for merging different cultivation regions by different restriction plates realizes cultivation region in exchanging area
Between between pathogen cells information communicate with each other, carry out cell-tocell transduction mechanism and signaling molecule screening study；Rubber pad
And second groove mutually completely cut off between each cultivation region when ensuring to cover ware lid, the setting of air hole makes culture dish that can both carry out
Aerobic culture can also carry out Anaerobic culturel；Filter membrane carries out sterilization absorption to air, reduces microbes in air to cultivation region
Interference.
Description of the drawings
Fig. 1 is preclinical medicine pathogen culture ware ware body schematic diagram of the present invention；
Fig. 2 is preclinical medicine pathogen culture ware ware lid schematic diagram of the present invention；
Fig. 3 is that preclinical medicine of the present invention is illustrated with the restriction plate that pathogen culture ware arc length is barrier circle periphery perimeter 3/4
Figure；
Fig. 4 is that preclinical medicine of the present invention is illustrated with the restriction plate that pathogen culture ware arc length is barrier circle periphery perimeter 1/2
Figure；
Fig. 5 is that preclinical medicine of the present invention is illustrated with the restriction plate that pathogen culture ware arc length is barrier circle periphery perimeter 1/4
Figure.
Reference sign：
1- ware bodies, 2- ware lids, the second grooves of 21-, 3- barrier circles, the first grooves of 31-, 32- restriction plates, 4- grades of items, 41- are carved
Degree, 5- marks, 6- air holes, 7- removable covers, 8- rubber pads.
Specific implementation mode
The specific embodiment of the present invention is described in detail in 1-5 below in conjunction with the accompanying drawings, it is to be understood that this hair
Bright protection domain is not restricted by specific implementation.
As shown in Figs. 1-5, a kind of preclinical medicine pathogen culture ware, including transparent ware body 1 and ware lid 2, ware body 1
Interior bottom is connected with barrier circle 3, is exchanging area in barrier circle 3, and the first groove 31 of annular is offered along 3 upper surfaces of barrier circle,
The restriction plate 32 of multiple and different arc length is slidably connected in first groove 31, winding 3 lateral wall of spacer ring is provided with 4 for connecting
The shelves item 4 of 3 and 1 madial wall of ware body is enclosed in barrier, and shelves item 4 is uniformly distributed around barrier circle 3, and barrier is enclosed 3 and ware body 1 by shelves item 4
Space between ware wall is separated into several cultivation regions, and the height of the height resistivity spacer ring 3 of shelves item 4 is high, restriction plate 32 and shelves item 4
Height it is identical, vertical scale 41 is arranged at intervals on shelves item 4, convenient for control culture dish in cultivate base unit weight, each cultivation region
Be both provided with mark 5 and air hole 6 on corresponding 1 lateral wall of ware body, and on 1 lateral wall of ware body, 6 edge of air hole is provided with
Removable cover 7, removable cover 7, which can cover, closes air hole 6；
The second groove 21 of annular is offered along 2 edge of ware lid, and 1 open edge of ware body is adapted with the second groove 21
It is sliding, in ware lid (2), it is provided with rubber pad 8 in 21 enclosing region of the second groove, rubber pad 8 can contact restriction plate 32 respectively
With shelves item 4, to completely cut off each cultivation region.
1 bottom of ware body is connected with stabilizer blade, and rubber sleeve is arranged with outside stabilizer blade, enhances the stability of ware body.
It is provided with filter membrane in air hole 6, and sterilization adsorbent is distributed on filter membrane, sterilization adsorbent is to be impregnated with to disappear
The active powdered carbon of venom, air carry out sterilization absorption, reduce interference of the microbes in air to cultivation region.
1/4,1/2 or the 3/4 of the radian 3 periphery perimeters of a length of barrier circle of restriction plate 32, by not when cultivating pathogen
The pathogen information of different cultivation regions is merged in exchanging area with restriction plate 32, realizes between cultivation region information phase between pathogen cells
It mutually links up, carries out cell-tocell transduction mechanism and signaling molecule screening study.
It should be noted that repeat in order to prevent, description of the invention preferred embodiment, but technology in the art
Personnel once know basic creative concept, then other change and modification can be made to embodiment.So appended right is wanted
It asks and is intended to be construed to include preferred embodiment and fall into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
God and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (5)
1. a kind of preclinical medicine pathogen culture ware, which is characterized in that described including transparent ware body (1) and ware lid (2)
Ware body (1) interior bottom is connected with barrier circle (3), is exchanging area in the barrier circle (3), is opened along barrier circle (3) upper surface
Equipped with the first groove (31), the restriction plate (32) of multiple and different arc length is slidably connected in first groove (31), around described
Barrier circle (3) lateral wall is equipped with several shelves items (4) for connecting described barrier circle (3) and the ware body (1) madial wall, described
The space that the barrier is enclosed between (3) and the ware body (1) ware wall is separated into several cultivation regions, and the shelves item by shelves item (4)
(4) height is higher than the height of the barrier circle (3), and the restriction plate (32) is identical as the height of shelves item (4), the shelves
It is arranged at intervals with vertical scale (41) on item (4), is each respectively provided on corresponding ware body (1) lateral wall in the cultivation region
Have a mark (5) and air hole (6), and on the ware body (1) lateral wall, the air hole (6) edge be provided with removable cover
(7), the removable cover (7), which can cover, closes the air hole (6)；
It is equipped with the second groove (21), and ware body (1) open edge and second groove along the ware lid (2) edge
(21) it is adapted sliding, on the ware lid (2), is provided with rubber pad (8) in the second groove (21) enclosing region, and
The rubber pad (8) can contact the restriction plate (32) and the shelves item (4) respectively.
2. preclinical medicine as described in claim 1 pathogen culture ware, which is characterized in that ware body (1) bottom is affixed
There is stabilizer blade, and rubber sleeve is arranged with outside the stabilizer blade.
3. preclinical medicine as described in claim 1 pathogen culture ware, which is characterized in that setting in the air hole (6)
There is filter membrane, and sterilization adsorbent is distributed on the filter membrane.
4. preclinical medicine as claimed in claim 3 pathogen culture ware, which is characterized in that the sterilization adsorbent is dipping
There is the active powdered carbon of thimerosal.
5. preclinical medicine as described in claim 1 pathogen culture ware, which is characterized in that the quantity of the shelves item (4) is 4
Item, and be uniformly distributed around barrier circle (3), (3) periphery perimeter is enclosed in a length of barrier of radian of the restriction plate (32)
1/4,1/2 or 3/4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810509042.6A CN108660066A (en) | 2018-05-24 | 2018-05-24 | Preclinical medicine pathogen culture ware |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810509042.6A CN108660066A (en) | 2018-05-24 | 2018-05-24 | Preclinical medicine pathogen culture ware |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108660066A true CN108660066A (en) | 2018-10-16 |
Family
ID=63777675
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810509042.6A Pending CN108660066A (en) | 2018-05-24 | 2018-05-24 | Preclinical medicine pathogen culture ware |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108660066A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109362654A (en) * | 2018-12-25 | 2019-02-22 | 甘肃农业大学 | A kind of Larva Harmonia Axyridis breeding apparatus and its method for breeding |
| CN115651841A (en) * | 2022-11-09 | 2023-01-31 | 昆明医科大学第一附属医院 | Device and method for co-culturing multiple cells of retina |
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| WO2007008609A2 (en) * | 2005-07-07 | 2007-01-18 | The Regents Of The University Of California | Methods and apparatus for cell culture array |
| CN202226863U (en) * | 2011-09-01 | 2012-05-23 | 山西医科大学 | Cell culture dish |
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| CN204939492U (en) * | 2015-05-27 | 2016-01-06 | 邬江 | A kind of multifunctional porous Tissue Culture Dish |
| CN205329034U (en) * | 2015-12-31 | 2016-06-22 | 山东省农作物种质资源中心 | A culture dish for microbial growth |
| CN106497768A (en) * | 2016-11-30 | 2017-03-15 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris culture dish of sealing ventilation |
| CN106591123A (en) * | 2016-12-19 | 2017-04-26 | 新疆畜牧科学院草业研究所 | Biological culture dish |
| CN206396199U (en) * | 2016-12-06 | 2017-08-11 | 四川国光农化股份有限公司 | A kind of Microbiological Culture Collection device |
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2018
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Patent Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998027431A1 (en) * | 1996-12-16 | 1998-06-25 | Wun Chun K | Motility channel pathogen detector and method of use |
| WO2007008609A2 (en) * | 2005-07-07 | 2007-01-18 | The Regents Of The University Of California | Methods and apparatus for cell culture array |
| CN202226863U (en) * | 2011-09-01 | 2012-05-23 | 山西医科大学 | Cell culture dish |
| CN202766533U (en) * | 2012-09-11 | 2013-03-06 | 青岛德瑞骏发生物科技有限公司 | Culture vessel |
| CN103031252A (en) * | 2012-12-20 | 2013-04-10 | 唐培培 | Multicellular co-culture dish and application method thereof |
| CN103205363A (en) * | 2013-04-19 | 2013-07-17 | 山东大学 | Cell culture dish |
| CN103320320A (en) * | 2013-06-06 | 2013-09-25 | 浙江硕华医用塑料有限公司 | Dish body of cell culture dish |
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| CN205329034U (en) * | 2015-12-31 | 2016-06-22 | 山东省农作物种质资源中心 | A culture dish for microbial growth |
| CN106497768A (en) * | 2016-11-30 | 2017-03-15 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris culture dish of sealing ventilation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109362654A (en) * | 2018-12-25 | 2019-02-22 | 甘肃农业大学 | A kind of Larva Harmonia Axyridis breeding apparatus and its method for breeding |
| CN115651841A (en) * | 2022-11-09 | 2023-01-31 | 昆明医科大学第一附属医院 | Device and method for co-culturing multiple cells of retina |
| CN115651841B (en) * | 2022-11-09 | 2023-11-10 | 昆明医科大学第一附属医院 | Device and method for co-culturing multiple cells of retina |
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| PB01 | Publication | ||
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181016 |
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| RJ01 | Rejection of invention patent application after publication |