CN204550568U - Iris type culture dish - Google Patents
Iris type culture dish Download PDFInfo
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- CN204550568U CN204550568U CN201520250456.3U CN201520250456U CN204550568U CN 204550568 U CN204550568 U CN 204550568U CN 201520250456 U CN201520250456 U CN 201520250456U CN 204550568 U CN204550568 U CN 204550568U
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Abstract
The utility model discloses a kind of iris type culture dish, comprise ware body and ware lid, the cover inner surface of described ware lid is provided with annular protrusion, in the groove between the sidewall that the sidewall of ware body is plugged in annular protrusion and ware lid, forms the ware body and ware lid that fasten; The internal surface of described ware body base plate is provided with vertical right-angled intersection diaphragm structure, and ware body inside is divided into four fan-shaped marker spaces by this diaphragm structure; The side-wall outer side of described ware body is provided with annular flange flange structure.The utility model is used for isolated Dual culture and the synthetic determination of the main pathogenic fungi in mammitis of cow milk sample, establishes cross diaphragm structure in ware body, can prepare four kinds of different substratum in same ware body; Projection is set in ware lid simultaneously, prevents the slip between ware lid and ware body, avoid causing microbiological contamination or smashing culture dish.
Description
Technical field
The utility model relates to Micro biological Tests technique with the apparatus field, especially a kind of iris type culture dish for the main pathogenic fungi separation and Culture in mammitis of cow milk sample.
Background technology
Culture dish is one of the most frequently used experiment consumptive material of life science, microbiological art, can be used for bacterium streak inoculation, colony morphological observation, bacterial count and bacteriostatic test etc.The Micro-Organism Culture Dish used in the inspection of current veterinary microorganism can only prepare a kind of substratum, and the ware intracoelomic cavity of culture dish arranges unreasonable, rare baffle design, and ware body and divider height improper, use in inconvenient test operation process.
Usually from sample is seeded in culture dish until complete cultivation, for avoiding culture microbiological contamination in ware, the ware lid of culture dish all can not be opened.When culture dish is placed in the plane such as desk or experiment table, because culture dish surface is more smooth and be circular, be therefore not easy very much to be picked up, and when taking, hand is easy to touch out upper cover, and causes microbiological contamination.And have certain space between the sidewall of ware lid and the sidewall of ware body, ware lid is easy to slide, and easily causes microbiological contamination or smashes culture dish.
Identification of Major Pathogens in Bovine Mastitis comprises streptococcus agalactiae, streptococcus aureus, streptococcus dysgalactiae and intestinal bacteria, need four streak inoculations same milk sample being carried out to blood agar plate, improvement Ai Dehua agar plate, general graceful agar plate and maconkey agar flat board in separation and Culture process, result judges to carry out synthetic determination according to the colony growth characteristic of milk sample in four kinds of isolation mediums.Micro-Organism Culture Dish in the past can only prepare a kind of solid separation culture medium, before sample streak inoculation, substratum need be divided in culture dish bottom surface with marking pen, and mark in zoning, same milk sample needs streak inoculation to a four different isolation medium, the colony growth characteristic of observing same milk sample needs simultaneously with reference to the result of four isolation mediums, cause the efficiency of bacteria distribution, cultivation in each milk sample lower, also the easy growth to bacterium in culture dish, observation and preliminary evaluation result cause and obscure erroneous judgement.
Utility model content
The utility model is for the deficiencies in the prior art, and propose a kind of iris type culture dish, structure is ingenious, easy to use.
In order to realize above-mentioned utility model object, the utility model provides following technical scheme: a kind of iris type culture dish, comprise ware body and ware lid, the cover inner surface of described ware lid is provided with annular protrusion, in groove between the sidewall that the sidewall of ware body is plugged in annular protrusion and ware lid, form the ware body and ware lid that fasten; The internal surface of described ware body base plate is provided with vertical right-angled intersection diaphragm structure, and ware body inside is divided into four fan-shaped marker spaces by this diaphragm structure; The side-wall outer side of described ware body is provided with annular flange flange structure.
Further, be provided with tick marks district outside ware body sidewall, bottom has large mark zone.
Further, the external diameter of described annular flange flange structure is greater than the external diameter of ware lid.
Further, the infall of described diaphragm structure is positioned at the center of ware body, and this central crossbar place is provided with middle jack-post and scale marks.
Compared with prior art, the utility model has the following advantages: in ware body, establish cross diaphragm structure, the isolation identification substratum of 4 kinds of different moietys can be poured into a mould in same culture dish simultaneously, be prepared into combined type solid medium, realize the isolated Dual culture of the main pathogenic fungi in mammitis of cow milk sample; Projection is set in ware lid simultaneously, prevents the slip between ware lid and ware body, avoid causing microbiological contamination or smashing culture dish.
This isolated culture dish saves test equipment and cost, greatly can improve the separation and Culture efficiency of Identification of Major Pathogens in Bovine Mastitis, avoid the cross infection of different sample.Secondly, by observing the colony growth characteristic of same milk sample on four kinds of different culture medias, result is very clear, can sharp separation, identify the classification of bacterium.
Accompanying drawing explanation
Fig. 1 is the sectional view of the isolated culture dish of the utility model;
Fig. 2 is the vertical view of ware body.
Embodiment
Be described in detail the utility model below in conjunction with accompanying drawing, the description of this part is only exemplary and explanatory, should not have any restriction to protection domain of the present utility model.
A kind of iris type culture dish as illustrated in fig. 1 and 2, comprises ware body 2 and ware lid 1, and ware body 2 is connected and composed by the lower end of annular sidewall 22 and the peripheral sealing of circular bottom plate 21, and ware lid 1 is connected and composed by the peripheral sealing of the upper end of annular sidewall 12 and circular cover 11.
Cover plate 11 internal surface of described ware lid 1 is provided with annular protrusion 13, in the groove between the sidewall 12 that the sidewall 22 of ware body 2 is plugged in annular protrusion 13 and ware lid 1, forms the ware body 2 and ware lid 1 that fasten; The internal surface of described ware body 2 base plate 21 is provided with vertical right-angled intersection diaphragm structure 23, and ware body 2 inside is divided into four fan-shaped marker spaces by this diaphragm structure 23; Described ware body sidewall 22 is outside upper is provided with annular flange flange structure 24.
Wherein the external diameter of annular flange flange structure 24 is greater than the external diameter of ware lid 1, and four fan-shaped marker spaces are not connected.The central crossbar place of decussation diaphragm structure 23 is the center of circular bottom plate 21, and this central crossbar place is provided with middle jack-post and scale marks, when being convenient to filling substratum, controls amount and the making sheet height of substratum; Outside the sidewall 22 of ware body 2, corresponding four fan-segmentation districts are provided with 4 tick marks districts, for marking four kinds of different substratum titles; One large mark zone is provided with, for marking milk sample number, inoculation time etc. at the place of bottom centre of ware body 2.
In groove between the sidewall 12 that the sidewall 22 of ware body 2 is plugged in annular protrusion 13 and ware lid 1, be conducive to ware lid 1 and ware body 2 better snaps together, prevent ware body 2 from slidably reciprocating in ware lid 1, microbiological contamination or smash culture dish when avoiding taking culture dish.
Vertical decussation diaphragm structure 23 thickness is 1.0mm, is highly 13mm, lower than the height of ware body 2; Because four fan-shaped marker spaces are mutually isolated, not connected, four kinds of different isolation mediums can be prepared in same plate, be conducive to rapid examination and the synthetic determination of test-results.
The outer wall of ware body sidewall 22 is provided with one annular flange flange structure 24, the culture dish that front of being convenient to take in experimental implementation process is placed.
The material (polypropylene) of high-temperature-resistant high-pressure-resistant selected by culture dish, and hardness is high, and quality is light, and glossy clear, is convenient to the observation of test-results, is independent ware body prepared by press forming, can repeated disinfection uses.
Applying culture dish of the present utility model, to carry out the step of milk sample separation and Culture as follows:
(1) culture dish sterilizing and medium preparing: culture dish kraft paper or tinfoil closely wrap up, 121 DEG C, sterilizing in 20 minutes, by culture dish dry for standby after sterilizing completes.
(2) 4 kinds of isolation mediums are prepared: 5% sheep blood nutrient agar, improvement Ai Dehua nutrient agar, general graceful nutrient agar and maconkey agar substratum.
(3) making sheet: in an aseptic environment, treat that culture medium solution naturally cools to about 56 DEG C, the plate of clean sterilizing is placed on Bechtop table top, 4 fan-shaped marker spaces of each plate add 4 kinds of different isolation mediums respectively, substratum thickness is made to reach about 4-5mm (to 5mm scale marks place), naturally cooling, to solidify, mark the title of corresponding substratum at ware body sidewall.
(4) streak inoculation and separation and Culture: carry out milk sample mark in plate bottom surface, shake up milk sample, streak inoculation is 4 kinds of different isolation mediums in plate, 4 isolation medium streak inoculations milk sample in a plate, 37 DEG C of incubators are cultivated, after 24 hours, observe the cultural characters of bacterium, qualification.
The above is only preferred implementation of the present utility model; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the utility model principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
Claims (4)
1. an iris type culture dish, comprises ware body and ware lid, it is characterized in that: the cover inner surface of described ware lid is provided with annular protrusion, in the groove between the sidewall that the sidewall of ware body is plugged in annular protrusion and ware lid, forms the ware body and ware lid that fasten; The internal surface of described ware body base plate is provided with vertical right-angled intersection diaphragm structure, and ware body inside is divided into four fan-shaped marker spaces by this diaphragm structure; The side-wall outer side of described ware body is provided with annular flange flange structure.
2. iris type culture dish as claimed in claim 1, it is characterized in that: be provided with tick marks district outside ware body sidewall, bottom has large mark zone.
3. iris type culture dish as claimed in claim 1, is characterized in that: the external diameter of described annular flange flange structure is greater than the external diameter of ware lid.
4. iris type culture dish as claimed in claim 1, it is characterized in that: the infall of described diaphragm structure is positioned at the center of ware body, this central crossbar place is provided with middle jack-post and scale marks.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520250456.3U CN204550568U (en) | 2015-04-23 | 2015-04-23 | Iris type culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520250456.3U CN204550568U (en) | 2015-04-23 | 2015-04-23 | Iris type culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204550568U true CN204550568U (en) | 2015-08-12 |
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ID=53825704
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520250456.3U Expired - Fee Related CN204550568U (en) | 2015-04-23 | 2015-04-23 | Iris type culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204550568U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110945114A (en) * | 2017-06-08 | 2020-03-31 | 维特罗莱夫公司 | Cover for culture dish |
| CN111235014A (en) * | 2020-03-16 | 2020-06-05 | 西南民族大学 | Culture dish and method for rapidly testing bacteriostatic ability of bacteriostatic substance by using culture dish |
| CN112574880A (en) * | 2020-12-10 | 2021-03-30 | 中国人民解放军陆军军医大学第二附属医院 | Lymphocyte separation culture dish |
-
2015
- 2015-04-23 CN CN201520250456.3U patent/CN204550568U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110945114A (en) * | 2017-06-08 | 2020-03-31 | 维特罗莱夫公司 | Cover for culture dish |
| CN111235014A (en) * | 2020-03-16 | 2020-06-05 | 西南民族大学 | Culture dish and method for rapidly testing bacteriostatic ability of bacteriostatic substance by using culture dish |
| CN112574880A (en) * | 2020-12-10 | 2021-03-30 | 中国人民解放军陆军军医大学第二附属医院 | Lymphocyte separation culture dish |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150812 Termination date: 20190423 |