CN112574880A

CN112574880A - Lymphocyte separation culture dish

Info

Publication number: CN112574880A
Application number: CN202011454622.3A
Authority: CN
Inventors: 邱远; 马海涛; 杜广胜; 肖卫东; 杨桦
Original assignee: Second Affiliated Hospital Army Medical University
Current assignee: Second Affiliated Hospital Army Medical University
Priority date: 2020-12-10
Filing date: 2020-12-10
Publication date: 2021-03-30

Abstract

The invention relates to the technical field of culture dishes, in particular to a lymphocyte separation culture dish, which comprises a storage mechanism, wherein the storage mechanism comprises a tank body with an open-type structure on the top surface, the bottom surface of the tank body is provided with a through hole, and a support column is connected in the through hole in a sliding manner; the lymphocyte separation culture dish is convenient for independent placement of the culture dish through the arranged storage mechanism, namely the arranged tank body and the sealing cover, and provides a closed placement environment for the culture dish; meanwhile, the arranged springs, the support columns, the support plates, the stop blocks and the like are used, namely, the culture dish is always positioned in the tank body under the action of the springs, and when the culture dish is taken, the culture dish can be protruded out of the tank body by pressing the culture dish downwards, so that the culture dish is convenient to take and use; this design is convenient for place the culture dish alone to for the culture dish provides the closed environment, formed the protection to the culture dish, this design also is convenient for take the culture dish simultaneously.

Description

Lymphocyte separation culture dish

Technical Field

The invention relates to the technical field of culture dishes, in particular to a lymphocyte separation culture dish.

Background

The culture dish is a laboratory vessel for culturing microorganisms or cells, consists of a flat disc-shaped bottom and a cover, and is generally made of glass or plastic; lymphocytes are one type of white blood cells, the smallest volume. The lymphocyte is produced by a lymph organ, mainly exists in circulating lymph fluid in a lymph duct, is an important cell component of the immune response function of an organism, and has important significance for human beings; at present, when the lymphocyte is separated, the lymphocyte needs to be transferred to a culture dish so as to keep the activity of the lymphocyte; in the prior art, the culture dish for placing the sample needs to be placed in a closed space, the existing culture dish lacks of an independent placing environment, the culture dish is often placed together by a plurality of culture dishes, confusion among the culture dishes is easy to cause, inconvenience is brought to research of scientific research personnel, and accordingly, the lymphocyte separation culture dish is provided.

Disclosure of Invention

The present invention is directed to a lymphocyte separation culture dish to solve the above problems in the background art.

In order to achieve the purpose, the invention provides the following technical scheme:

a lymphocyte separation culture dish comprises a storage mechanism, wherein the storage mechanism comprises a tank body with an open-type structure on the top, a through hole is formed in the bottom surface of the tank body, a support is connected in the through hole in a sliding manner, a spring is sleeved on the support, a fixing plate is tightly bonded at the top end of the support, a plurality of support plates which are distributed in an annular equal interval manner are tightly bonded on the outer wall of the circumference of the fixing plate, a stop block is tightly bonded at the tail end of the top surface of each support plate, a support is tightly bonded at the bottom end of the support, two ends of the spring respectively abut against the bottom surface of the tank body and the top surface of the support, and a plurality of support legs which are distributed in an annular equal; a sealing cover is arranged above the tank body and is in threaded connection with the tank body, a groove is formed in the top surface of the sealing cover, and an observation lens is embedded in the groove; the internal culture dish that is equipped with of jar, the culture dish is located a plurality ofly between the dog, a plurality of culture chamber that are the equidistant distribution of annular are seted up to the top surface of culture dish, and two are adjacent closely bond between the culture chamber has the baffle.

As a preferable technical scheme of the invention, two symmetrical handles are closely adhered to the circumferential outer wall of the tank body.

As a preferable technical scheme of the invention, a plurality of annular ribs distributed at equal intervals are closely adhered on the circumferential outer wall of the sealing cover.

As a preferable technical scheme of the invention, the overall shape of the stop block is arc-shaped, and the outer wall of the culture dish is attached to the inner wall of the stop block.

In a preferred embodiment of the present invention, the observation lens is a convex lens made of transparent glass.

As a preferable technical scheme of the invention, two symmetrical bulges are closely adhered on the circumferential outer wall of the culture dish.

Compared with the prior art, the invention has the beneficial effects that: the lymphocyte separation culture dish is convenient for independent placement of the culture dish through the arranged storage mechanism, namely the arranged tank body and the sealing cover, and provides a closed placement environment for the culture dish; meanwhile, the arranged springs, the support columns, the support plates, the stop blocks and the like are used, namely, the culture dish is always positioned in the tank body under the action of the springs, and when the culture dish is taken, the culture dish can be protruded out of the tank body by pressing the culture dish downwards, so that the culture dish is convenient to take and use; this design is convenient for place the culture dish alone to for the culture dish provides the closed environment, formed the protection to the culture dish, this design also is convenient for take the culture dish simultaneously.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic diagram of the overall explosive structure of the present invention;

FIG. 3 is a schematic structural view of a can body according to the present invention;

FIG. 4 is a schematic view of a portion of the storage mechanism of the present invention;

FIG. 5 is a schematic view showing the structure of a culture dish according to the present invention.

The various reference numbers in the figures mean:

1. a storage mechanism; 11. a tank body; 111. a through hole; 12. a handle; 13. a pillar; 14. a spring; 15. a fixing plate; 16. a support plate; 17. a stopper; 18. a support; 19. a support leg;

2. a sealing cover; 21. a groove; 22. a rib; 23. observing the lens;

3. a culture dish; 31. a culture chamber; 32. a partition plate; 33. and (4) protruding.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the equipment or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.

Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.

The invention provides a technical scheme that:

referring to fig. 1-5, a lymphocyte separation culture dish comprises a storage mechanism 1, wherein the storage mechanism 1 comprises a tank body 11 with an open top, a through hole 111 is formed in the bottom surface of the tank body 11, a support column 13 is slidably connected in the through hole 111, a spring 14 is sleeved on the support column 13, a fixing plate 15 is tightly bonded at the top end of the support column 13, a plurality of support plates 16 which are annularly and equidistantly distributed are tightly bonded on the circumferential outer wall of the fixing plate 15, a stop block 17 is tightly bonded at the tail end of the top surface of each support plate 16, a support 18 is tightly bonded at the bottom end of the support column 13, two ends of the spring 14 respectively abut against the bottom surface of the tank body 11 and the top surface of the support 18, and a plurality of support legs 19 which are annularly and equidistantly distributed are; a sealing cover 2 is arranged above the tank body 11, the sealing cover 2 is in threaded connection with the tank body 11, a groove 21 is formed in the top surface of the sealing cover 2, and an observation lens 23 is embedded in the groove 21; be equipped with culture dish 3 in jar body 11, culture dish 3 is located between a plurality of dogs 17, and a plurality of culture chamber 31 that are the equidistant distribution of annular are seted up to culture dish 3's top surface, and closely bond between two adjacent culture chamber 31 has baffle 32.

In this embodiment, two symmetrical handles 12 are closely adhered to the outer circumferential wall of the pot 11, and the handles 12 are arranged to facilitate downward pressing of the pot 11, thereby facilitating the removal of the culture dish 3 from the pot 11.

In this embodiment, the circumference outer wall of sealing lid 2 goes up close bonding has a plurality of fins 22 that are the equidistant distribution of annular, and the setting of a plurality of fins 22 is convenient for increase hand and sealing lid 2's frictional force, is convenient for rotate sealing lid 2 to its opening and shutting.

In this embodiment, the whole shape of dog 17 is the arc, and the outer wall of culture dish 3 is laminated mutually with the inner wall of dog 17, and setting up of dog 17 has played spacing effect to culture dish 3, is convenient for fix culture dish 3 on fixed plate 15.

In this embodiment, the observation lens 23 is a convex lens made of transparent glass, and the arrangement of the observation lens 23 facilitates real-time observation of the condition in the culture dish 3.

In this embodiment, two symmetrical protrusions 33 are closely adhered to the outer circumferential wall of the culture dish 3, and the protrusions 33 are arranged to facilitate the removal of the culture dish 3 from the vessel 11.

When the lymphocyte separation culture dish of this embodiment is used, a user firstly unscrews the sealing cover 2, then the user holds the handles 12 at both sides with hands and presses downward, the tank body 11 moves downward under the action of pressure, the through hole 111 slides along the support column 13, the spring 14 is compressed, at this time, the culture dish 3 protrudes from the opening on the top surface of the tank body 11, and the user can take off the culture dish.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A lymphocyte separation culture dish, includes storage mechanism (1), its characterized in that: the storage mechanism (1) comprises a tank body (11) with an open-top structure, a through hole (111) is formed in the bottom surface of the tank body (11), a support column (13) is connected in the through hole (111) in a sliding mode, a spring (14) is sleeved on the support column (13), a fixing plate (15) is tightly bonded to the top end of the support column (13), a plurality of support plates (16) which are distributed in an annular shape at equal intervals are tightly bonded to the outer wall of the circumference of the fixing plate (15), a stop block (17) is tightly bonded to the top end of each support plate (16), a support seat (18) is tightly bonded to the bottom end of the support column (13), two ends of the spring (14) respectively abut against the bottom surface of the tank body (11) and the top surface of the support seat (18), and a plurality of support legs (19) which are distributed in an annular shape; a sealing cover (2) is arranged above the tank body (11), the sealing cover (2) is in threaded connection with the tank body (11), a groove (21) is formed in the top surface of the sealing cover (2), and an observation lens (23) is embedded in the groove (21); be equipped with culture dish (3) in jar body (11), culture dish (3) are located a plurality ofly between dog (17), a plurality of culture chamber (31) that are the equidistant distribution of annular are seted up to the top surface of culture dish (3), and two are adjacent closely bond between culture chamber (31) has baffle (32).

2. The lymphocyte separation culture dish according to claim 1, wherein: two symmetrical handles (12) are closely adhered to the circumferential outer wall of the tank body (11).

3. The lymphocyte separation culture dish according to claim 1, wherein: the outer wall of the circumference of the sealing cover (2) is closely adhered with a plurality of circular convex edges (22) which are distributed at equal intervals.

4. The lymphocyte separation culture dish according to claim 1, wherein: the whole shape of dog (17) is the arc, the outer wall of culture dish (3) is laminated with the inner wall of dog (17) mutually.

5. The lymphocyte separation culture dish according to claim 1, wherein: the observation lens (23) is a convex lens made of transparent glass.

6. The lymphocyte separation culture dish according to claim 1, wherein: two symmetrical bulges (33) are tightly adhered on the circumferential outer wall of the culture dish (3).