CN220317835U - Multicellular non-contact co-culture device - Google Patents
Multicellular non-contact co-culture device Download PDFInfo
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- CN220317835U CN220317835U CN202321799218.9U CN202321799218U CN220317835U CN 220317835 U CN220317835 U CN 220317835U CN 202321799218 U CN202321799218 U CN 202321799218U CN 220317835 U CN220317835 U CN 220317835U
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- 238000003501 co-culture Methods 0.000 title claims abstract description 38
- 238000004113 cell culture Methods 0.000 claims abstract description 100
- 239000012528 membrane Substances 0.000 claims abstract description 35
- 239000000463 material Substances 0.000 claims abstract description 16
- 230000000975 bioactive effect Effects 0.000 claims abstract description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 description 76
- 230000009286 beneficial effect Effects 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- 230000003993 interaction Effects 0.000 description 8
- 238000001727 in vivo Methods 0.000 description 6
- 210000004748 cultured cell Anatomy 0.000 description 3
- 238000013401 experimental design Methods 0.000 description 3
- 102000004127 Cytokines Human genes 0.000 description 2
- 108090000695 Cytokines Proteins 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000002775 capsule Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
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Abstract
The utility model aims to provide a multicellular non-contact co-culture device which consists of an external large vessel, a telescopic carrying plate, a plurality of cell culture cells and a dish cover, wherein each cell culture cell is round, the inner diameter of each cell culture cell can be consistent or inconsistent, the bottom of each cell culture cell is made of a semipermeable membrane material, the aperture of the semipermeable membrane is less than or equal to 1 mu m, and only bioactive molecules can pass but cells cannot pass, so that the contact influence among cells is isolated; the two ends of the telescopic carrying plate are provided with protruding parts with the thickness of 1mm so as to be capable of being carried on the side wall of the external large vessel, the thickness of the side wall of the large vessel is more than or equal to 1mm, the lowest point of the telescopic carrying plate is about 5mm away from the inner bottom surface of the external large vessel, the cell culture cell is arranged on the telescopic carrying plate, and the bottom of the cell is about 2mm away from the inner bottom surface of the external large vessel.
Description
Technical Field
The utility model relates to the field of basic cell experiments, in particular to a multicellular non-contact co-culture experiment.
Background
The cell co-culture technology can simulate in vivo environment to a great extent so as to better observe the interaction between cells and culture environment, and discuss the action mechanism and possible action targets of drugs by detecting the relationship between different cytokines. It was found that not only signal transduction and mass exchange can be performed by direct contact between cells, but also interaction can be performed by secreted cytokines. The non-contact co-culture of cells eliminates the influence of the conditional cells themselves on the target cells, thereby better observing the effect of the conditional cells on the target cells. However, at present, cell non-contact co-culture devices such as a Transwell chamber and the like only can allow two kinds of cells to be co-cultured, and no cell non-contact co-culture device for 3 or more kinds of cells exists. Therefore, how to solve the above-mentioned problems and meet the experimental design of non-contact co-culture of multiple cells, so that the cell experiment is more in line with the microenvironment where the cells are located in vivo, and improvement of the existing non-contact co-culture device is needed.
Disclosure of Invention
The utility model aims to provide a multicellular non-contact co-culture device which adopts a design of a plurality of cell culture chambers, can meet the requirement of non-contact co-culture of 3 or more cells, improves the limitation that the traditional co-culture device such as Transwell and the like can only co-culture two cells, and is more beneficial to observing the interaction among a plurality of cells so as to enable the cell experiment to be more in accordance with in-vivo research.
The technical scheme of the utility model is as follows: a multicellular non-contact co-culture device, characterized in that: the cell culture device consists of an external large vessel, a telescopic carrying plate, a plurality of cell culture cells and a vessel cover, wherein the telescopic carrying plate is positioned inside the large vessel, two ends of the telescopic carrying plate are provided with convex parts with the diameter of 1mm, the telescopic carrying plate can be carried on the side wall of the large vessel, the length of any telescopic carrying plate can be adjusted, the telescopic carrying plate is movably connected with the side wall of the large vessel, the thickness of any telescopic carrying plate is 1mm, the distance between the telescopic carrying plate and the bottom surface of the large vessel is about 5mm, the cell culture cells are positioned inside the large vessel, the cell culture cells are cylindrical, the upper end of the cell culture cells are opened, the cell culture chamber bottom is sealed with semi-permeable membrane material, cell culture chamber's upper mouth edge all with outwards extend about 1mm, cell culture chamber's semi-permeable membrane material can be PC membrane or PET membrane, cell culture chamber is all placed on scalable embarkation board, wantonly cell culture chamber is swing joint with scalable embarkation board, cell culture chamber bottom is about 2mm apart from big household utensils inner bottom surface, big household utensils shape can be cylindrical, big household utensils lateral wall thickness is greater than or equal to 1mm, the dish lid is located the upper portion of big household utensils, dish lid and big household utensils can gomphosis each other, be swing joint between dish lid and the big household utensils.
Further, the plurality of cell culture chambers are independent and have a semipermeable membrane sealing structure at the lower end.
Further, the two ends of the telescopic carrying plate are respectively provided with a protruding part with the length of 1mm, the telescopic carrying plate can be erected on the side wall of the external large vessel, the distance between the telescopic carrying plate and the inner bottom surface of the large vessel is about 5mm, and the length and the number of the telescopic carrying plate can be freely adjusted according to the size and the number of the cell culture cells.
Furthermore, the semipermeable membrane material at the bottom of the cell culture chamber can be PC membrane or PET membrane, the pore diameter of the semipermeable membrane is less than or equal to 1 mu m, and the semipermeable membrane material can allow the free passage of bioactive substances, but the cells cannot pass through the semipermeable membrane material.
Further, the cell culture chamber can be placed on the telescopic carrying plate, and is movably connected with the telescopic carrying plate, and the bottom surface of the cell culture chamber is about 2mm away from the inner bottom surface of the large vessel.
The plurality of cell culture chambers may be equal in size or different in size, and the plurality of retractable mounting plates may be equal in length or different in length.
The number of the plurality of cell culture chambers is 2 to 4, and the number of the plurality of retractable mounting plates is 2 to 3.
The utility model has the beneficial effects that the device is a multicellular non-contact co-culture device which adopts a design of a plurality of cell culture chambers, can meet the requirement of non-contact co-culture of 3 or more cells, improves the limitation that the traditional co-culture device such as Transwell can only co-culture two cells, is more beneficial to observing the interaction among a plurality of cells, and enables the cell experiment to be more in line with the in-vivo research. The inner diameter of each cell culture chamber can be consistent or inconsistent, the bottom of each cell culture chamber is sealed by a semi-permeable membrane material, the colors of the cell culture chambers can be various, the cultured cells are mainly convenient to mark, each cell culture chamber is independently arranged and is not contacted with each other, the sizes of the cell culture chambers can be different according to the types of cultured cells, the number interval of the cell culture chambers in the external large vessel is 2-4, thus the non-contact co-culture of 3 or more cells can be met, a telescopic carrying plate is also carried in the large vessel, the length of the telescopic carrying plate can be freely adjusted according to the sizes of the cell culture chambers, the two ends of the telescopic carrying plate are provided with protruding parts with the thickness of 1mm so as to be arranged on the side wall of the large vessel, the thickness of the side wall of the large vessel is more than or equal to 1mm, the cell culture chambers are arranged on the telescopic carrying plate, the bottom of the cell culture chambers are closer to the inner bottom surface of the large vessel than the telescopic carrying plate, the cell culture chambers can be allowed to freely flow, the liquid is not influenced by the telescopic carrying plate, the cover and the large vessel can be embedded with the large vessel, the cell culture chamber can be beneficial to the gas culture, and the evaporation requirements of the liquid can be reduced; the device has simple structure, convenient use and low cost, can meet the requirement of non-contact co-culture of 3 or more cells, is more beneficial to observing the interaction among various cells, widens the experimental design range of the non-contact co-culture of the cells, and ensures that the cell experiment is more in line with in-vivo research.
Drawings
FIG. 1 is a front view of the present utility model
FIG. 2 is a schematic structural view of a cell culture chamber according to the present utility model
FIG. 3 is a schematic view showing the connection structure of the telescopic carrying plate and the large vessel according to the present utility model
FIG. 4 is a schematic side view of the whole of the utility model without the capsule
Wherein: 1. external large vessel 2, telescopic carrying plate
3. Cell culture chamber 4, dish lid
Detailed Description
The following is a brief description of embodiments of the present utility model with reference to the accompanying drawings.
The multicellular non-contact co-culture device as shown in fig. 1, 2, 3 and 4 is characterized in that: the cell culture dish comprises an external large vessel 1, a telescopic carrying plate 2, a plurality of cell culture small chambers 3 and a dish cover 4, wherein the telescopic carrying plate 2 is positioned inside the large vessel 1, two ends of the telescopic carrying plate 2 are provided with protruding 1mm parts, the telescopic carrying plate 2 can be carried on the side wall of the large vessel 1, any length of the telescopic carrying plate 2 can be adjusted, the telescopic carrying plate 2 and the side wall of the large vessel 1 are movably connected, any thickness of the telescopic carrying plate 2 is 1mm, the lowest point of the telescopic carrying plate 2 is about 5mm away from the inner bottom surface of the large vessel 1, the cell culture small chambers 3 are positioned inside the large vessel 1, the cell culture small chambers 3 are all cylindrical, the upper ends of the cell culture small chambers 3 are opened, the bottom ends of the cell culture small chambers 3 are sealed by semi-permeable membrane materials, the upper opening edges of the cell culture small chambers 3 are uniformly and outwards extended by about 1mm, the semi-permeable membrane materials of the cell culture small chambers 3 can be PC membranes or membranes, the cell culture small chambers 3 can be placed on the inner bottom surface of the large vessel 1mm, the cell culture small chambers 2 are movably connected with the large vessel 1, the cell culture small chambers 2 are positioned on the inner bottom surface of the large vessel 1, the cell culture small chambers 2 are movably connected with the large vessel 1, the cell culture small chambers 4 are movably arranged on the inner bottom surface of the large vessel 1, and the cell culture small chamber 2 is positioned on the inner bottom surface of the large vessel 1, and the cell culture dish is positioned between the cell culture small chamber 2 and the cell culture dish 1 and the cell cover 1 is positioned inside the cell cover 1. The cell culture chambers 3 are independent and have a semipermeable membrane material sealing structure at the lower end. The two ends of the telescopic carrying plate 2 are respectively provided with a protruding part with the length of 1mm, the telescopic carrying plate can be erected on the side wall of the external large vessel 1, the distance between the telescopic carrying plate 2 and the inner bottom surface of the large vessel 1 is about 5mm, and the length and the number of the telescopic carrying plates 2 can be freely adjusted according to the size and the number of the cell culture cells 3. The semi-permeable membrane material at the bottom of the cell culture chamber 3 can be PC membrane or PET membrane, the pore diameter of the semi-permeable membrane is less than or equal to 1 μm, and the semi-permeable membrane material can allow bioactive substances to pass freely, but cells cannot pass. The cell culture chamber 3 is placed on the telescopic carrying plate 2 and is movably connected with the telescopic carrying plate 2, and the bottom surface of the cell culture chamber is about 2mm away from the inner bottom surface of the large vessel 1. The cell culture chambers 3 may be equal in size or different in size, and the retractable mounting plates 2 may be equal in length or different in length. The number of the cell culture chambers 3 is 2 to 4, and the number of the telescopic carrying plates 2 is 2 to 3.
Embodiments are described below: the co-culture device is designed by adopting a plurality of cell culture chambers, can meet the non-contact co-culture of 3 or more cells, improves the limitation that the existing device can only perform non-contact co-culture of two cells, is more beneficial to observing the interaction among various cells, widens the application range of cell co-culture experiments, and enables the living environment of the cells in the co-culture experiments to be more fit with the micro environment in the body; the cell culture device mainly comprises an external large vessel 1, a telescopic carrying plate 2, a plurality of cell culture cells 3 and a vessel cover 4, wherein each cell culture cell 3 is independent, the lower end of each cell culture cell is of a semi-permeable membrane sealing structure, the cell culture cells 3 are movably connected with the telescopic carrying plate 2 so as to facilitate convenient removal when cells are replaced, the cell culture cells 3 can be the same in size or different in size, the cell culture cells can be freely selected according to specific cell types, one cell culture cell 3 is white in color, the other cell culture cells 3 can be deeper in color, thus being beneficial to cells needing strict light-proof culture, the names of the cultured cells can be marked, the phenomenon of confusion is prevented when the cells are cultured, in addition, the distance between the bottom of each cell culture cell 3 and the bottom of the large vessel 1 is equal, and is about 2mm, so that the culture solution can be used for the cells in the cell culture cells 3, the number of the cell culture cells 3 in the large vessel 1 is 2-4, and the simultaneous culture requirements of various cells can be met; the length of each telescopic carrying plate 2 can be freely adjusted, the thickness is 1mm, the two ends of each telescopic carrying plate are respectively provided with a part protruding for 1mm so as to be capable of being erected on the side wall of the large vessel 1, the thickness of the side wall of the large vessel 1 is more than or equal to 1mm, so that the protruding part of each telescopic carrying plate 2 cannot exceed the large vessel 1, in addition, the distance between the lowest surface of each telescopic carrying plate 2 and the bottom surface of the large vessel 1 is about 5mm, the distance is larger than the distance between the cell culture small chamber 3 and the inner bottom surface of the large vessel 1, the purpose is to prevent the telescopic carrying plates 2 from contacting culture solution and interfering interaction among cells, the length of each telescopic carrying plate 2 can be equal, the connection between the large vessel 1 and the large vessel 1 is flexible, the position of the telescopic carrying plate 2 is conveniently adjusted to be beneficial to observing cells, the number interval of the telescopic carrying plate 2 in the large vessel 1 is 2-3, a plurality of cell culture cells 3 can be carried, the large vessel 1 is an external main body of the whole device and is also a cell culture dish, a plurality of types of cells can be cultured, when a plurality of types of cells are subjected to non-contact co-culture, induced cells are cultured in the large vessel 1, cells to be differentiated are cultured in the cell culture cells 3, and therefore the effect of lower induced cells on upper cells to be differentiated is observed; the dish cover 4 is positioned at the uppermost layer of the whole device, can be perfectly embedded with the large vessel 1, is used for covering the microenvironment where the cells are cultured, and can reduce the evaporation of the cell culture solution; the device has simple structure, convenient use and low cost, can meet the requirement of non-contact co-culture of 3 or more cells, is more beneficial to observing the interaction among various cells, widens the experimental design range of the non-contact co-culture of the cells, and ensures that the cell experiment is more in line with in-vivo research.
In the description of the present utility model, it should be noted that the positional or positional relationship indicated by the terms such as "bottom", "inner", "outer", "top", "bottom", "end", etc. are based on the positional or positional relationship shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or element in question must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The foregoing describes one embodiment of the present utility model in detail, but the description is only a preferred embodiment of the present utility model and should not be construed as limiting the scope of the utility model. All equivalent changes and modifications within the scope of the present utility model are intended to be covered by the present utility model.
Claims (7)
1. A multicellular non-contact co-culture device, characterized in that: the cell culture dish comprises an external large vessel, a telescopic carrying plate, a plurality of cell culture cells and a dish cover, wherein the telescopic carrying plate is positioned inside the large vessel, two ends of the telescopic carrying plate are provided with protruding parts with the thickness of 1mm, the length of the telescopic carrying plate can be adjusted, the telescopic carrying plate and the side wall of the large vessel are movably connected, the thickness of the telescopic carrying plate is 1mm, the lowest point of the telescopic carrying plate is 5mm away from the bottom surface of the large vessel, the cell culture cells are positioned inside the large vessel, the cell culture cells are cylindrical, the upper ends of the cell culture cells are opened, the bottom ends of the cell culture cells are sealed by semi-permeable membrane materials, the edge of the upper opening of the cell culture cells is uniformly extended to the left or right of the large vessel, the semi-permeable membrane materials of the bottom surface of the cell culture cells can be PC membranes or membranes, the cell culture cells are placed on the telescopic carrying plate, the cell culture cells are 1mm away from the bottom surface of the large vessel, the bottom surface of the cell culture cells are movably connected with the bottom surface of the large vessel, the bottom surface of the cell culture dish is 2mm, the shape of the large vessel is equal to or more than the bottom surface of the large vessel, and the shape of the large vessel is equal to the large vessel is 2mm, and the shape of the large vessel is equal to the large vessel, and the large vessel is connected with the dish, and the dish is 2mm and the dish, and the large dish is connected by the dish and the dish.
2. The multicellular non-contact co-culture apparatus of claim 1 wherein: the cell culture chambers are independent, and the lower ends of the cell culture chambers are semi-permeable membrane sealing structures.
3. The multicellular non-contact co-culture apparatus of claim 1 wherein: the two ends of the telescopic carrying plate are respectively provided with a protruding part with the length of 1mm, the telescopic carrying plate can be erected on the side wall of the external large vessel, the distance between the telescopic carrying plate and the inner bottom surface of the large vessel is about 5mm, and the length and the number of the telescopic carrying plate can be freely adjusted according to the size and the number of the cell culture cells.
4. The multicellular non-contact co-culture apparatus of claim 1 wherein: the semi-permeable membrane material at the bottom of the cell culture chamber can be PC membrane or PET membrane, the pore diameter of the semi-permeable membrane is less than or equal to 1 μm, and the semi-permeable membrane material can allow bioactive substances to pass freely, but cells cannot pass.
5. The multicellular non-contact co-culture apparatus of claim 1 wherein: the cell culture chamber can be placed on the telescopic carrying plate and is movably connected with the telescopic carrying plate, and the distance between the bottom surface of the cell culture chamber and the inner bottom surface of the large vessel is about 2 mm.
6. The multicellular non-contact co-culture apparatus of claim 1 wherein: the cell culture chambers may be equal in size or different in size, and the retractable mounting plates may be equal in length or different in length.
7. The multicellular non-contact co-culture apparatus of claim 1 wherein: the number of the cell culture chambers is 2-4, and the number of the telescopic carrying plates is 2-3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321799218.9U CN220317835U (en) | 2023-07-10 | 2023-07-10 | Multicellular non-contact co-culture device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321799218.9U CN220317835U (en) | 2023-07-10 | 2023-07-10 | Multicellular non-contact co-culture device |
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| CN220317835U true CN220317835U (en) | 2024-01-09 |
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| CN202321799218.9U Active CN220317835U (en) | 2023-07-10 | 2023-07-10 | Multicellular non-contact co-culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110616140A (en) * | 2019-10-28 | 2019-12-27 | 上海国睿生命科技有限公司 | Cell co-culture dish |
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2023
- 2023-07-10 CN CN202321799218.9U patent/CN220317835U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110616140A (en) * | 2019-10-28 | 2019-12-27 | 上海国睿生命科技有限公司 | Cell co-culture dish |
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