CN207528747U - A kind of zebrafish embryo microwell plate arranges adding set - Google Patents
A kind of zebrafish embryo microwell plate arranges adding set Download PDFInfo
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- CN207528747U CN207528747U CN201721454100.7U CN201721454100U CN207528747U CN 207528747 U CN207528747 U CN 207528747U CN 201721454100 U CN201721454100 U CN 201721454100U CN 207528747 U CN207528747 U CN 207528747U
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Abstract
The utility model is related to a kind of zebrafish embryo microwell plates to arrange adding set, including vacuum chamber locular wall, embryo addition room locular wall and porous barrier, embryo addition room locular wall is fixed at the connection sealed around of porous barrier top surface and forms the embryo addition room without top box shape with porous barrier top surface, the vacuum chamber locular wall and the locular wall integrated molding of embryo addition room, and the closing connection of the bottom surface of the vacuum chamber locular wall and porous barrier forms bottomless box-like space, and the bottomless box-like space is covered by film when in use and forms vacuum chamber, into the matrix arrangement and embryo of perforation embryo addition room and vacuum chamber is equipped in the porous barrier and captures through-hole.Compared with prior art, the utility model can in the case that do not influence embryo's normal growth and development disposably complete microwell plate zebrafish embryo add work, can be with Reusability；And significantly improve conventional efficient.
Description
Technical field
The utility model is related to Bioexperiment product technical fields, and in particular to a kind of zebrafish embryo microwell plate arrangement adds
Feeder apparatus.
Background technology
Now, the model organism for being widely used in drug and toxicologic study is mainly mouse, drosophila, nematode etc.
Biology, these small-sized biologicals can keep organism complete, and the reflection of observation analysis organism moves, cycle,
It is immune to wait indices, so as to compensate for be equally used for the cell line of screening and detection and tissue culture technique due to its environment with
Technology requirement is high, the deficiency of cell is difficult to replicate and Testing index is single etc..These model organisms can be divided into vertebra and move
Object and two major class of invertebrate, in vertebrate, the example that fish especially zebra fish is used for model organism is the widest
It is general.Zebra fish is low as a kind of raising expense, rearing conditions are simple, and individual size is smaller can mass rearing in the lab
Model organism has obtained more and more concerns in recent years, becomes a kind of drug and the representativeness biology of toxicologic study.Together
When again since the yield of its embryo is big, embryonic development is fast and synchronous development can meet the big research of sample requirements, and develop
The features such as optical clarity of process organ is high causes zebrafish embryo and juvenile fish to become extensive pharmacology and toxicologic study
Ideal model or even the alternative model for being hopeful further to become adult fish classics ecotoxicity assay.
Contradiction, the analysis of small model organism in a manner of high-throughput and high-content, such as fish embryo and juvenile fish still
It is a challenging task.This is mainly due to the inherent limitation of routine techniques and metazoa organism are more difficult
Caused by carrying out laboratory automation.Although some are in progress in terms of the counting of embryo, embryo selects, exposure, and culture is main
It is performed manually by, and cultivates, exposure is mostly carried out with detection in 6,24,96,384 hole microwell plates, when this will expend a large amount of
Between with the energy of scientific research personnel or even influence the accuracy of experiment.And it is taken also the most most in the application of zebrafish embryo
Exactly zebrafish embryo is put into microwell plate for crucial step, 96 or 384 are mostly used again in order to reduce the influence between embryo
Embryo in a manner that dropper draws embryo, is put into microwell plate by hole microwell plate, is put into per hole after an embryo and drug or latent
It is exposed in the solution mixing of noxious material.It well imagines, millimetre-sized zebrafish embryo is moved into microwell plate will expend greatly
The time of amount, one expertly scientific research personnel complete a 96 hole microwell plates addition take around 30~40 minutes, complete 384
The time that the bed board work of hole microwell plate is spent is up to several hours.Moreover, in a manner that dropper draws embryo, not only may be used
Mechanical injuries can be caused to embryo, and during embryo is moved into microwell plate, have a certain amount of Embryo Culture
Liquid is also added into microwell plate together, and solution of these culture solutions in the future with drug or other substances to be detected mixes, dilute
Liquid to be detected is released, no standard measure and diluted degree varies with each individual, this just causes the reliability of experimental result certain shadow
It rings.
The once technological development such as applied robot and image identification of the Research Team of University of California in Los Angeles are a kind of high
The full-automatic Screening Platform of flux, being drawn and placed using mechanical arm realizes the full-automatic of embryo's addition by the way of embryo, obtains
Fabulous application effect, saves a large amount of scientific research time.But it is higher there is also cost, popularize the big limitation of difficulty.
Therefore a kind of efficient, safety is developed, the microwell plate adding set of economic zebrafish embryo just seems very urgent
With necessity.However up to the present there are no one can take into account at a high speed, the high-throughput product with low price comes out.
Utility model content
The purpose of this utility model be exactly in order to overcome the problems of the above-mentioned prior art and provide it is a kind of it is efficiently quick,
The economic zebrafish embryo microwell plate arrangement adding set of cleaning.
The purpose of this utility model can be achieved through the following technical solutions：A kind of zebrafish embryo microwell plate arrangement adds
Feeder apparatus, the adding set include vacuum chamber locular wall, embryo addition room locular wall and porous barrier, embryo addition room room
Wall is fixed at the connection sealed around of porous barrier top surface and forms the embryo without top box shape with porous barrier top surface and adds
Room, the vacuum chamber locular wall and the locular wall integrated molding of embryo addition room, and the bottom of the vacuum chamber locular wall and porous barrier
Face closure connects to form bottomless box-like space, and covers the bottomless box-like space by film when in use and form vacuum chamber,
Into the matrix arrangement and embryo of perforation embryo addition room and vacuum chamber is equipped in the porous barrier and captures through-hole.This practicality
It is novel to connect vacuum chamber with embryo addition room by setting vacuum chamber, and by embryo's capture through-hole, it when in use, will
Embryo medium containing zebrafish embryo is poured into embryo addition room, due to air pressure subatmospheric in vacuum chamber, so
Embryo medium can flow into embryo and capture in through-hole, be captured in through-hole so as to take zebrafish embryo to embryo, as all embryos
After accommodating zebrafish embryo in capture through-hole, standard size microwell plate is tipped upside down on porous barrier, and by whole device
Then overturning stops vacuum chamber and generates negative pressure, in this way, zebrafish embryo is with regard to that can fall into the micropore of standard size microwell plate.
The utility model device can disposably complete micropore in the case where not influencing embryo's normal growth and development without mechanical damage
The zebrafish embryo addition work of plate, can be with Reusability.Sample-adding can not only add in embryo manually by the way of artificial,
It can be added to culture solution is more or less in microwell plate, but by the way of vacuum, culture solution can be drawn into vacuum pump, institute
It can be fallen into embryo when inverted, and culture solution chance will not enter microwell plate, so as to reduce diluted possibility.
Aspirating hole there are one being set in the vacuum chamber locular wall, is connect with aspirating hole with vacuum pump, then opens vacuum
Pump, vacuum chamber is in negative pressure state at this time.
The embryo captures the negative pressure channel that through-hole includes embryo's capture groove of upper end and lower end is vertically arranged, institute
It states negative pressure channel to connect with vacuum chamber, and the aperture of negative pressure channel is less than the grain size of zebrafish embryo.Embryo captures groove
The taper or cylinder that aperture is gradually reduced from top to bottom, as long as can guarantee that its inner space can accommodate a zebra
Fish embryo.The aperture of negative pressure channel is less than the grain size of zebrafish embryo, so as to ensure that it is negative that zebrafish embryo will not be inhaled into
In balancing gate pit, but rest on embryo and capture in groove.In addition, embryo captures the size of groove according to experiment demand, it is general only
An embryo can be accommodated, multiple embryos is avoided to fall into same microwell plate micropore, influence experimental result, when certain special experiments need
Two zebrafish embryos are captured simultaneously, then the inside that embryo can be captured to groove expands.
Four angles of the porous barrier top surface are equipped with to block the fixing groove for setting standard size microwell plate.Setting is solid
Determine slot, when preventing from overturning whole device, with porous barrier position offset occurs for standard size microwell plate, so as to can not normally turn
Move embryo.The shape of fixing groove and the edge of standard size microwell plate match, and are close to the edge of standard size microwell plate.
Described four sides of porous barrier are equipped with gentle slope, and the height on the gentle slope is equipped with embryo's capture through-hole less than intermediate
Partial height.Gentle slope is set, is to be captured after through-hole captures zebrafish embryo in all embryos, it may be convenient to will
Embryo medium and extra zebrafish embryo are removed to gentle slope, it is avoided to rest on embryo and is captured near through-hole, to later stage embryo
Transfer have an impact.
The arrangement of embryo's capture through-hole matches with the pore arrangement in standard size microwell plate in the porous barrier.
The porous barrier color is dark color.Since the color of zebrafish embryo is light color, so the face of porous barrier
Color is arranged to dark color, can relatively easily judge whether there is zebrafish embryo in embryo captures groove.
Compared with prior art, the beneficial effects of the utility model are embodied in following several respects：
(1) the utility model device can be in the case where not influencing embryo's normal growth and development without mechanical damage one
Secondary property completes the zebrafish embryo addition work of microwell plate, can be with Reusability；
(2) it realizes and zebrafish embryo is quickly added into microwell plate, significantly improve conventional efficient；
(3) apparatus structure is simple, cheap, and cleaning is convenient, will not because of it is complicated, be difficult to clear up, occur thin
The situation of bacterium infecting embryo.
Description of the drawings
Fig. 1 is the positive dimensional structure diagram of the utility model device；
Fig. 2 is the vertical view of Fig. 1；
Fig. 3 is Fig. 1 side sectional views；
Fig. 4 is the structure diagram that the utility model embryo captures through-hole；
Fig. 5 is the structure diagram of standard size microwell plate.
Wherein, 1 is embryo addition room, and 2 be vacuum chamber, and 3 be porous barrier, and 4 capture through-hole for embryo, and 5 be negative pressure
Room locular wall, 6 be aspirating hole, and 7 be embryo addition room locular wall, and 8 be fixing groove, and 9 be embryo's capture region, and 10 be gentle slope, and 11 be mark
Object staff cun microwell plate, 12 be micropore, and 13 capture groove for embryo, and 14 be negative pressure channel, and 15 be adhesive membrane.
Specific embodiment
Elaborate below to the embodiment of the utility model, the present embodiment using technical solutions of the utility model before
It puts and is implemented, give detailed embodiment and specific operating process, but the scope of protection of the utility model is unlimited
In following embodiments.
Embodiment 1
A kind of zebrafish embryo microwell plate arranges adding set, structure as shown in Figure 1, 2, 3, mainly by embryo addition room
1, the porous barrier 3 for capturing through-hole 4 between vacuum chamber 2 and embryo addition room 1 and vacuum chamber 2 with embryo forms, each portion
/ to produce once molding be fixedly connected；7 bottom of embryo addition room locular wall is closed by covering one layer of adhesive membrane 15,
Vacuum chamber 2 captures through-hole 4 by embryo and is connect with porous barrier 3, and porous barrier 3 is carried in 1 side quadrangle of embryo addition room
Microwell plate fixing groove 8, vacuum chamber locular wall 7 are provided with aspirating hole 6
Porous barrier 3 includes the gentle slope 10 of intermediate embryo's capture region 9 and surrounding.It is set in embryo's capture region 9
Embryo capture through-hole 4 and the arrangement of micropore 12 in standard size microwell plate 11 and match, when standard size microwell plate 11 is buckled to
On porous barrier 3, the embryo on porous barrier captures through-hole 4 and 96 micropores 12 of standard size microwell plate 11 are mutually right
Should, wherein, the structure of standard size microwell plate 11 is as shown in Figure 5.
Embryo captures 4 upper end of through-hole and is equipped with the embryo's capture groove 13 for being slightly larger than embryo's cross-sectional area, and embryo captures groove
The negative pressure channel 14 for being less than embryo's cross-sectional area after 13 bottom openings with a cross-sectional area is connect, the connection of 14 other end of negative pressure channel
Vacuum chamber, as shown in Figure 4.
In use, with 2 bottom of an adhesive membrane 15 covering vacuum chamber, so that it becomes a closed cavity, uses vacuum pump
Vacuum pump is opened after being connect with the aspirating hole 6 on vacuum chamber locular wall 5, vacuum chamber 2 is in negative pressure state at this time, due to embryo
4 both ends of capture through-hole connect vacuum chamber 2 and embryo addition room 1 respectively, so the embryo of 1 side of embryo addition room captures through-hole
4 can obtain suction, and the embryo of addition is captured and is stuck in 13 bottom of embryo's capture groove, and embryo medium then can be along bottom
Gap be drawn into vacuum chamber 2.After embryo's capture groove 13 that all embryos are captured on through-hole 4 is filled embryo, close
Vacuum pump collects surplus embryos and embryo medium, standard size microwell plate 11 is buckled into microwell plate fixing groove 8, makes micropore
The embryo that 12 opening face embryos capture through-hole 4 captures groove 13, overturns the arrangement addition of zebrafish embryo microwell plate simultaneously later
Device and standard size microwell plate 11 make vacuum chamber 2 upward, blow and beat 1 side of vacuum chamber of porous barrier 3, final zebra
Fish embryo's total number is fallen into 11 in standard size microwell plate.
Certainly, embryo addition room, vacuum chamber, the connection mode of porous barrier can be the flexible connections such as card slot connection.This
Embryo captures groove and can also be used semicircle, half elliptic or other suitable shapes, negative pressure channel be alternatively cuboid etc. other
Applicability shape.It can also according to the microwell plate type (96 holes, 384 holes etc.) used and not for capturing the porous barrier of embryo
Different pore size distributions with brand are revised as adaptable structure.Light color, other applicable colors of transparent grade can also be used in color.Material
It is alternatively other applicability materials such as resin, plastics, rubber.It is such transformation all fall within the scope of protection of the utility model it
It is interior.
Claims (7)
1. a kind of zebrafish embryo microwell plate arranges adding set, which is characterized in that the adding set include vacuum chamber locular wall,
Embryo addition room locular wall and porous barrier, embryo addition room locular wall are fixed at the sealed around of porous barrier top surface
It connects and forms the embryo addition room without top box shape, the vacuum chamber locular wall and embryo addition room locular wall with porous barrier top surface
It is integrally formed, and the closing connection of the bottom surface of the vacuum chamber locular wall and porous barrier forms bottomless box-like space, and using
When the bottomless box-like space covered by film form vacuum chamber, be equipped with into matrix arrangement in the porous barrier and penetrate through
The embryo of embryo addition room and vacuum chamber captures through-hole.
2. a kind of zebrafish embryo microwell plate arrangement adding set according to claim 1, which is characterized in that described is negative
Aspirating hole there are one being set in balancing gate pit's locular wall.
A kind of 3. zebrafish embryo microwell plate arrangement adding set according to claim 1, which is characterized in that the embryo
The negative pressure channel that tire capture through-hole includes embryo's capture groove of upper end and lower end is vertically arranged, the negative pressure channel and negative pressure
Power room connects, and the aperture of negative pressure channel is less than the grain size of zebrafish embryo.
4. a kind of zebrafish embryo microwell plate arrangement adding set according to claim 1, which is characterized in that described is more
Four angles of hole partition board top surface are equipped with to block the fixing groove for setting standard size microwell plate.
5. a kind of zebrafish embryo microwell plate arrangement adding set according to claim 1, which is characterized in that described is more
Partition board four sides in hole are equipped with gentle slope, and the height on the gentle slope is less than the intermediate height for being equipped with embryo and capturing throughhole portions.
6. a kind of zebrafish embryo microwell plate arrangement adding set according to claim 1, which is characterized in that described is more
The arrangement of embryo's capture through-hole matches with the pore arrangement in standard size microwell plate in the partition board of hole.
7. a kind of zebrafish embryo microwell plate arrangement adding set according to claim 1, which is characterized in that described is more
Hole partition board color is dark color.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721454100.7U CN207528747U (en) | 2017-11-03 | 2017-11-03 | A kind of zebrafish embryo microwell plate arranges adding set |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721454100.7U CN207528747U (en) | 2017-11-03 | 2017-11-03 | A kind of zebrafish embryo microwell plate arranges adding set |
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| CN207528747U true CN207528747U (en) | 2018-06-22 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107966581A (en) * | 2017-11-03 | 2018-04-27 | 同济大学 | A kind of zebrafish embryo microwell plate arranges adding set |
| CN108982826A (en) * | 2018-09-28 | 2018-12-11 | 广东工业大学 | Test device and test method of a kind of zebra fish detection nano particle to embryotoxicity |
-
2017
- 2017-11-03 CN CN201721454100.7U patent/CN207528747U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107966581A (en) * | 2017-11-03 | 2018-04-27 | 同济大学 | A kind of zebrafish embryo microwell plate arranges adding set |
| CN107966581B (en) * | 2017-11-03 | 2023-08-29 | 同济大学 | Zebra fish embryo microplate arrangement adding device |
| CN108982826A (en) * | 2018-09-28 | 2018-12-11 | 广东工业大学 | Test device and test method of a kind of zebra fish detection nano particle to embryotoxicity |
| CN108982826B (en) * | 2018-09-28 | 2024-03-26 | 广东工业大学 | Test device and test method for detecting embryotoxicity of nano particles by zebra fish |
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