CN212533020U

CN212533020U - Special culture dish of embryo time difference monitoring system

Info

Publication number: CN212533020U
Application number: CN202020302887.0U
Authority: CN
Inventors: 李平
Original assignee: Guangzhou Pinzhi Medical Equipment Co ltd
Current assignee: Guangzhou Pinzhi Medical Equipment Co ltd
Priority date: 2020-03-12
Filing date: 2020-03-12
Publication date: 2021-02-12
Anticipated expiration: 2030-03-12

Abstract

The utility model relates to a special culture dish of embryo time difference monitoring system, including the culture dish main part, the culture dish main part comprises diapire and the lateral wall round the vertical setting of diapire outer fringe, wherein the diapire is circular or oval, and diapire and lateral wall form the cavity that can hold liquid. The inside protruding ring that is connected with the diapire that is equipped with of culture dish main part and around the peripheral a plurality of circular arc be used for abluent well, a plurality of micropores that are used for placing the embryo are seted up to the bottom of protruding ring. The culture dish can culture embryos in the same environment together through the design; meanwhile, a plurality of micropores are arranged in the middle convex circular ring, so that the position of the embryo is limited in a specific micropore in the culture process, the embryo can be observed, detected and photographed in the whole process in real time by matching with time difference culture monitoring equipment, and the embryo with the best quality is selected under the condition of reducing the interference on the embryo and the culture environment as much as possible; meanwhile, the evaluation of the development potential of the embryo is more objective and standardized, and the human error is reduced.

Description

Special culture dish of embryo time difference monitoring system

Technical Field

The utility model belongs to cell culture dish field, concretely relates to special culture dish of embryo time difference monitoring system.

Background

Embryo culture refers to culturing the obtained early embryo in an in vitro artificial environment to monitor the development of the embryo or the possibility of artificial intervention in a certain development process, and the conventional embryo culture is mostly carried out on a common culture dish and needs to be put in a special culture box for culture; embryo analysis requires manually transferring embryos to a microscope under an atmospheric environment, and the embryos need to be placed back into an incubator after microscopic examination. The operation causes fluctuation of air pressure, temperature and humidity, the embryo exposure chance is increased, and the development images of a plurality of time points can only be obtained by conventional embryo culture analysis and evaluation; because the indexes selected in the laboratory and the time and skill of technicians for evaluating the embryos are different, the evaluation result has greater subjectivity and limitation.

At the present stage, a new time difference culture monitoring technology is provided, and the embryo development process can be continuously photographed; in the time difference incubator, an embryologist does not need to take the embryos out of the incubator for observation, and people can observe the development conditions of the embryos intuitively and uninterruptedly only through images in a laboratory; the whole process is free from interference and is closer to the culture condition of a parent body; meanwhile, the time difference monitoring technology enables the embryo development potential evaluation to be more objective and standardized, and reduces human errors. However, the culture dish used for matching with the embryo time difference monitoring system cannot realize that a plurality of embryos are cultured in the same environment together.

SUMMERY OF THE UTILITY MODEL

In order to overcome prior art's not enough, the utility model aims to provide a special culture dish of embryo time difference monitoring system, including the culture dish main part, the culture dish main part comprises diapire and the lateral wall round the vertical setting of diapire outer fringe, and inside protruding ring and a plurality of abluent well that are used for of being equipped with and being connected with the diapire of culture dish main part, and a plurality of micropores that are used for placing the embryo are seted up to the bottom of protruding ring. The culture dish can meet the requirements of a special dish for monitoring embryo culture through the design, and can realize that a plurality of embryos are cultured in the same environment together; meanwhile, the time difference monitoring technology is used for independently and continuously photographing the embryos, so that the embryo development interference can be reduced, and the embryo development process information can be obtained in real time.

The purpose of the utility model is realized by adopting the following technical scheme:

embryo time difference monitoring system special culture dish, including the culture dish main part, the culture dish main part by the diapire and round the lateral wall of the vertical setting of diapire outer fringe is constituteed, culture dish main part inside be equipped with protruding ring and a plurality of abluent well that are used for that the diapire is connected, a plurality of micropores that are used for placing the embryo are seted up to the bottom of protruding ring, make the embryo cultivate jointly in same environment.

Furthermore, four or five micropores are in one group, and a plurality of groups of micropores are arranged and distributed in a matrix.

Further, the opening shape of the micro-hole is circular.

Further, the purge well inlay in the diapire, the purge well has four and surrounds the protruding ring interval sets up.

Further, the lateral wall is equipped with a plurality of inside sunken and is used for fixing the recess of the special culture dish of embryo time difference monitoring system, the recess is the semicircle column type and along the inboard interval setting of lateral wall.

Furthermore, the outer side wall surrounding the culture dish main body is provided with a holding part which is convenient for a human hand to hold, and the holding part is in a wavy line shape.

Further, the bottom wall is circular or elliptical.

Furthermore, the culture dish special for the embryo time difference monitoring system is made of plastics.

Furthermore, the special culture dish for the embryo time difference monitoring system further comprises a dish cover matched with the special culture dish, and the dish cover is provided with three convex ventilating grooves.

Compared with the prior art, the beneficial effects of the utility model reside in that:

the utility model discloses a special culture dish of embryo time difference monitoring system, including the culture dish main part, the culture dish main part comprises diapire and the lateral wall round the vertical setting of diapire outer fringe, wherein the diapire is circular or oval, and diapire and lateral wall form the cavity that can hold liquid. A raised circular ring connected with the bottom wall and a plurality of wells for cleaning are arranged in the culture dish main body, and a plurality of micropores for placing embryos are formed in the bottom of the raised circular ring, so that the embryos are cultured together in the same environment; meanwhile, a plurality of micropores are arranged in the middle convex circular ring, so that the position of the embryo is limited in a specific micropore in the culture process, and the embryo with the best quality is selected under the condition of reducing the interference of the embryo and the culture environment as much as possible by being matched with time difference culture monitoring equipment to carry out whole-process observation, monitoring and photographing in real time; meanwhile, the evaluation of the development potential of the embryo is more objective and standardized, and the human error is reduced.

Drawings

FIG. 1 is a schematic structural diagram of a culture dish specially used for an embryo time difference monitoring system according to the preferred embodiment of the present invention;

FIG. 2 is a front view of a culture dish for embryo time difference monitoring system according to the preferred embodiment of the present invention;

FIG. 3 is a cross-sectional view of a culture dish for embryo time difference monitoring system according to the preferred embodiment of the present invention.

FIG. 4 is a schematic structural view of the preferred embodiment of the dish cover special for the embryo time difference monitoring system

In the figure: 1. a culture dish main body; 2. a raised circular ring; 3. cleaning the well; 4. micropores; 5. a groove; 6. a grip portion; 7. a dish cover; 8. and (4) ventilating grooves.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that the embodiments or technical features described below can be arbitrarily combined to form a new embodiment without conflict.

The utility model discloses embryo time difference monitoring system special culture dish is shown in fig. 1-3, including culture dish main part 1, culture dish main part 1 comprises diapire and the lateral wall round the vertical setting of diapire outer fringe, wherein the diapire is circular or oval, and diapire and lateral wall form the cavity that can hold liquid. The inside protruding ring 2 and a plurality of cleaning well 3 that are used for cultivating the cleaning of subassembly that are connected with the diapire that are equipped with of culture dish main part 1, a plurality of micropores 4 that are used for placing the embryo have been seted up to the bottom of protruding ring 2, make the embryo cultivate in same environment jointly. Meanwhile, a plurality of micropores are arranged in the middle convex ring 2, so that the position of the embryo is limited in a specific micropore in the culture process, and the embryo with the best quality is selected under the condition of reducing the interference of the embryo and the culture environment as much as possible by being matched with time difference culture monitoring equipment to carry out whole-process observation, monitoring and photographing in real time; meanwhile, the evaluation of the development potential of the embryo is more objective and standardized, and the human error is reduced.

Preferably, the bottom wall and the side wall of the main body 1 of the culture dish in the present embodiment are preferably formed in a shape similar to that of a conventionally used culture dish (35mm x 12mm), i.e., a culture dish adapted to be used in a common culture box at the same time.

In this embodiment, the four micropores 4 are in a group and distributed in a matrix, the number of the micropores 4 may be set to be a plurality of groups for simultaneous observation and detection, each group is composed of four or more holes formed adjacent to each other, and the groups may not be adjacent to each other. The outer edge shape of the opening of the minute hole 4 is not particularly linear, and is preferably circular. Generally, when culturing embryos or the like, the medium for the embryos is dropped into a petri dish and then oil is dropped to prevent the medium from drying. In this embodiment, the raised ring 2 is located at the centre of the culture dish body 1 and all the wells 4 are separated from other areas within the culture dish body 1 by a raised inner ring surrounding them, avoiding dispersion of the embryo culture medium while ensuring that the embryo culture is not contaminated by other areas.

Preferably, in order to distinguish and identify different embryos in the microwells 4, a mark for distinguishing is further provided around the microwells 4, and the mark can be a number or a letter. It is convenient to observe research and register information.

In this embodiment, a large washing well 3 is provided in the other region of the culture dish body 1 where the middle convex ring 2 is not provided. The wells are at least four in number and are spaced around the periphery of the raised ring 2. The cleaning well 3 can be embedded in the bottom wall or can be protruded from the bottom wall to form an inner wall. The purpose of the washing well 3 is mainly to wash embryos or applicable accessory components during embryo culture, such as a suction pipe, a suction head and the like. Ensuring that the cultured embryos are not contaminated.

Preferably, a holding part 6 which is convenient for being held by hands is arranged on the outer side wall surrounding the culture dish main body 1, and the holding part 6 is in a wavy line shape, so that the frictional resistance can be increased. The culture dish can be held tightly by workers, and the culture dish is not easy to slide.

Preferably, the lateral wall is equipped with a plurality of inside sunken and be used for fixing the recess 5 of the special culture dish of embryo time difference monitoring system, recess 5 is the semicircle column type and follows the inboard interval of lateral wall sets up. The height of this recess 5 is preferably no higher than the height of the peripheral grip portion 6. The purpose of this groove 5 is to stabilize the culture dish inside the apparatus; or for determining the orientation of the culture dish when in use.

The culture dish special for the embryo time difference monitoring system in the embodiment is made of plastic. The plastic material includes but is not limited to one or more of polystyrene PS, polycarbonate PC, polyethylene terephthalate PET, polymethyl methacrylate PMMA. The culture dish accessible injection moulding in this scheme.

Preferably, this scheme still be equipped with time difference equipment special culture dish complex ware lid 7, ware lid 7 is equipped with three bellied ventilative groove 8, after being convenient for cover the ware lid, the culture dish exchanges with external air.

The culture dish in this scheme is generally produced by the injection moulding process, puts into the alcohol of certain concentration earlier after the production is accomplished and cleans the back and carries out surface hydrophilic treatment. When the embryo culture is carried out, the processed embryo is sucked by a Pasteur sucker and put into the micropore 4 in the center of the culture dish after the molding and surface treatment, the embryo is respectively arranged at the corresponding positions according to the number of the embryos, and the position information can be determined according to the letters above the pores and the numbers at the left. Then, the culture oil is poured into the convex ring 2 to prevent the culture medium from volatilizing. The capsule lid 7 is closed. After the embryo is placed, the culture dish is held by hand to be arranged in a fixed groove 5Placing into a gas environment (37 deg.C; 6% CO)₂Air) in the incubator; after the embryo starts to crack, the shooting is started by a plurality of observation lenses arranged in the device and is continued until the required time.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention cannot be limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are all within the protection scope of the present invention.

Claims

1. Embryo time difference monitoring system special culture dish, its characterized in that: including the culture dish main part, the culture dish main part by the diapire and round the lateral wall of the vertical setting of diapire outer fringe is constituteed, culture dish main part inside be equipped with protruding ring and a plurality of abluent well that are used for that the diapire is connected, a plurality of micropores that are used for placing the embryo are seted up to the bottom of protruding ring, make the embryo cultivate jointly in same environment.

2. The special culture dish for embryo timing difference monitoring system according to claim 1, wherein: four or five micropores are in a group, and a plurality of groups of micropores are arranged and distributed in a matrix.

3. The special culture dish for embryo timing difference monitoring system according to claim 1, wherein: the opening of the micropore is circular.

4. The special culture dish for embryo timing difference monitoring system according to claim 1, wherein: the washing well inlay in the diapire, the washing well has four and surrounds protruding ring interval sets up.

5. The special culture dish for embryo timing difference monitoring system according to claim 1, wherein: the lateral wall is equipped with a plurality of inside sunken and is used for fixing the recess of the special culture dish of embryo time difference monitoring system, the recess is the semicircle column type and along the inboard interval setting of lateral wall.

6. The special culture dish for embryo timing difference monitoring system according to claim 1, wherein: the outer side wall of the culture dish main body is provided with a holding part which is convenient for a human hand to hold, and the holding part is wavy and linear.

7. The special culture dish for embryo timing difference monitoring system according to claim 1, wherein: the bottom wall is circular or oval.

8. The special culture dish for embryo timing difference monitoring system according to claim 1, wherein: the special culture dish for the embryo time difference monitoring system is made of plastics.

9. The special culture dish for embryo timing difference monitoring system according to claim 1, wherein: the special culture dish for the embryo time difference monitoring system further comprises a dish cover matched with the special culture dish, and the dish cover is provided with three convex ventilating grooves.