CN106754358B - A kind of cell co-cultivation flask convenient for transferring cells - Google Patents

Abstract

The invention discloses a cell co-cultivation culture bottle which is convenient for transferring cells. The body of the culture bottle is divided into two culture chambers by a spacer. The airtight cover that seals the mouth of the bottle, the spacer is provided with a communication mechanism that can communicate with the two cultivation chambers, the communication mechanism is provided with a sealing mechanism that can cut off the passages of the two cultivation chambers, and the side wall of the cultivation chamber is also provided with The variable pressure part that can change the air pressure inside the cultivation chamber. The bottom surface of the cultivation chamber is a double-layer structure. The upper structure is an elastic film located in the cultivation chamber, and the lower structure is a rigid support plate located below the elastic film. Close the airtight cover to seal the bottle. In addition, when the pressure changing part of the cultivation chamber generates negative pressure in the cultivation chamber, the elastic film bulges to form a closed cavity between the elastic film and the rigid support plate. The cell culture bottle can realize the co-cultivation of cells, and at the same time, it is convenient to transfer the cells in the bottle.

Description

A kind of cell co-cultivation flask convenient for transferring cells

technical field

The invention relates to a cell culture device, in particular to a cell co-cultivation flask for convenient cell transfer.

Background technique

A cell culture flask is a device used to culture cells. According to the material, it can be divided into glass and plastic, and the shape of the bottom is square, rectangular, triangular, etc., and there are 5 to 500ml of bottles to choose from according to the volume of the bottle. Because the cell attachment area provided by its bottom surface is larger than that of conventional culture dishes, it is often used for large-scale cultivation of cells. Cell culture bottles are generally provided with a bottle body, and a small bottle mouth is arranged on the side wall of the bottle body. Bottle cap, the bottle cap of the domestic cell culture bottle is mostly a closed airtight cap, which can be used for airtight culture. At the same time, the bottle cap and the bottle mouth are generally connected by threads, and the bottle cap can be loosened to ensure the gas in the bottle and the gas in the environment. Exchange, can also be used for open culture. The cells in the cell culture flask are cultured adherently at the bottom of the bottle body. Various operations during the cell culture process, such as changing the medium, cell subculture, etc., require the tip of the dropper or pipette to be extended from the small mouth of the bottle. Into the bottle for operation, and in order to avoid contamination during the operation, the dropper and pipette tip should try to avoid touching the bottle mouth, the operation process is more troublesome, especially in the process of transferring cells such as cell passage, the cells are separated from the culture bottle For the bottom surface, the culture medium in the bottle needs to be aspirated first, and the cell surface is washed with PBS to remove the residual culture medium, then added with transferase to digest for a certain period of time, and then new medium with serum is added to stop the digestion, and the culture medium is sucked up with a dropper to continuously wash, Blow the bottom of the culture bottle to make the cells fall off the bottom of the bottle to form a cell suspension. However, due to the small mouth of the culture bottle, the movement of the dropper in the culture bottle is greatly restricted, and it is difficult to re-wash the culture medium into the bottle. The cells at the corners of the bottom surface of the body bring difficulties to the entire cell transfer process. In addition, the interaction of different cells is a common occurrence in the biological world. Co-cultivation of cells is a basic means to study cell interactions. With the increase in the depth of cell biology research, the interaction between cells is actually a kind of information exchange between cells. This information exchange has a great impact on cells, affecting their proliferation, differentiation, and substance secretion. Therefore, cell co-cultivation has a profound impact on cell biology research. However, cell co-cultivation is not simply placing different cells in the same culture chamber for culture. Different cells are mixed and cultured. The growth cycle of different cells is different. Competing for the living space of another kind of cells too quickly makes it inconvenient to observe; it is also inconvenient to distinguish mixed culture cells, which leads to certain difficulties in in situ identification of cells. To achieve co-cultivation of cells.

Contents of the invention

The purpose of the invention is to improve the existing products and provide a cell co-cultivation culture bottle which is convenient for cell transfer. This kind of culture bottle can realize the exchange and exchange of cell secretions at any time according to the needs of the experiment, realize the information exchange between cells, realize the co-cultivation of cells, and can also be used when transferring cells The adherent cells at the bottom of the culture flask are easily detached from the bottom of the entire flask, which makes the experimental operation that needs to transfer cells more convenient.

In order to achieve the above object, the technical scheme provided by the invention is as follows:

A cell co-culture culture bottle for convenient cell transfer, the bottle body of the culture bottle is divided into two culture chambers by a spacer, each culture chamber is provided with a culture bottle mouth on the side wall, and the culture bottle mouth is connected to a sealed bottle The sealing cover of the mouth, the spacer is provided with a communication mechanism that can communicate with the two cultivation chambers, and the communication mechanism is provided with a sealing mechanism that can cut off the passages of the two cultivation chambers, and the side wall of the cultivation chamber is also provided with a variable The pressure changing part inside the cultivation chamber. The bottom surface of the cultivation chamber is a double-layer structure. The upper structure is an elastic film located in the cultivation chamber, and the lower structure is a rigid support plate located below the elastic film. The pressure changing part of the chamber makes the elastic membrane bulge when negative pressure is generated in the cultivation chamber, forming a closed cavity between the elastic membrane and the rigid support plate.

The cell culture bottle divides the inside of the bottle into two culture chambers through a spacer with a switchable communication mechanism, and the two culture chambers are respectively provided with bottle mouths with airtight covers. When the communication mechanism on the spacer is closed, the two culture chambers The culture chambers are independent of each other, and the different cells cultured in the two culture chambers do not affect each other. According to the growth requirements of the cells themselves and the needs of the experiment, conventional cells such as liquid exchange (medium replacement) and passage can be independently completed through the bottle mouth of each culture chamber. Experimental manipulation without affecting cell growth in another culture chamber. In the operation that needs to transfer cells such as cell subculture, after completing the step of transferase digestion and adding a sufficient amount of medium, it is only necessary to repeatedly change the air pressure in the culture chamber by closing the airtight cap and sealing the bottle body by using the variable pressure part of the culture chamber. The pressure changing part reduces the air pressure in the culture chamber, and the elastic film on the bottom of the culture chamber stretches and bulges under the action of negative pressure. At the same time, the entire elastic film (including the edge) to which the cells adhere fluctuates with the change of air pressure, causing the cells digested by transferase to fall off from the elastic film. The disturbance of the airflow in the bottle, the airflow acts on the medium, so that the cells in the medium are fully dispersed to form a cell suspension. In the traditional operation, use a dropper to absorb the medium and continuously wash and blow the bottom of the culture bottle to make the cells fall off from the bottom of the bottle to obtain the cell suspension. The movement of the bottle body is greatly restricted, and it is difficult for the culture medium to re-brush to the corner position of the bottom surface of the bottle body. At the same time, when the experiment requires the communication of cells between the two culture chambers, the communication mechanism on the spacer part can be opened, and the medium in the two culture chambers can diffuse and exchange with each other through the passage of the communication mechanism, so as to realize the communication and exchange of cell secretions, thereby Realize the information exchange between cells to achieve the purpose of cell co-cultivation. If adherent cells are cultivated in both culture chambers, different cells are still in their respective culture chambers and do not mix with each other during the process of cell information exchange and interaction. , to facilitate observation and observation of cell growth and shape changes. At the same time, during the process of cell co-cultivation, close the airtight lids of the two culture chambers to seal the culture flasks, and use the pressure-changing mechanism to slightly fluctuate the elastic films on the bottom of the two culture chambers for an appropriate time. , It can also speed up the mutual mixing of culture media between culture chambers, enhance the interaction between cell secretions, and be more conducive to experimental observation.

Specifically, the spacer is arranged in the center of the bottle, and the bottle is equally divided into left and right culture chambers, and the mouth of each culture chamber is arranged on the side wall away from the spacer. Parts are provided on the front side wall of each culture chamber.

Specifically, the spacer includes a partition that divides the interior of the bottle into two cultivation chambers, and the bottom of the partition is provided with a through hole that communicates with the two cultivation chambers near the bottom of the bottle, and the partition also includes an insert that can close the through hole The partition is provided with a slot that matches the insert, the port of the slot is located on the top of the partition, the slot runs through the through hole, the insert is inserted into the partition from the port of the slot, and the spacer An insert positioning mechanism is also provided to fix the position of the insert to close or open the through hole, and the spacer is also provided with a sealing mechanism to prevent the culture chamber from leaking.

Specifically, the port on one side of the through hole is covered with a microporous membrane, and the membrane pore size of the microporous membrane is less than 50 microns. The pore size setting of the microporous membrane allows the culture medium to enter freely, but does not allow the cells to enter and exit, so that even when the cells cultured in the two culture chambers are cultured in suspension, different cells can still be kept in separate culture chambers without mixing with each other, which is convenient for cell experiments Operation and observation, observation.

Specifically, the insertion piece positioning mechanism includes a baffle installed on the top of the insertion piece, the size of the bottom surface of the baffle is larger than the size of the top surface of the insertion piece connected to it, the baffle is located outside the bottle body, the bottle body The upper surface is provided with an opening corresponding to the port at the top of the slot. The shape and size of the opening are the same as the port at the top of the slot. The mechanism is a rubber sealing ring installed at the opening of the upper surface of the bottle, and the rubber sealing ring is closely attached to the side wall of the insert; the baffle is provided with an elastic supporting mechanism and a pressing mechanism, and the elastic supporting mechanism is in a natural state The baffle is supported above the bottle body, and the insert connected to the baffle is limited in the slot above the through hole. The through hole is opened, and the two culture chambers are connected. The medium in the culture chamber is exchanged with each other through the through hole to realize cell Information exchange; when the compression mechanism can compress the elastic support mechanism to compress and deform, so that the baffle is close to the upper surface of the bottle body, under the pressure of the compression mechanism, the baffle drives the insert to insert into the bottom of the slot to close the through hole. At this time, the cell The channel for medium exchange is closed, the two culture rooms are relatively independent, and the different cells cultured in the two culture rooms do not affect each other. According to the growth requirements of the cells themselves and the experimental needs, the medium exchange and addition can be completed independently through the bottle mouth of each culture room. Routine cell experiment operations such as sampling and passage.

Specifically, the elastic support mechanism of the baffle is two springs symmetrically arranged at the bottom of the baffle, one end of the spring is connected to the bottom surface of the baffle, and the other end of the spring is connected to the upper surface of the bottle body. , the baffle is supported above the bottle body, the insert connected to the baffle is limited in the slot above the through hole, and the through hole is opened; the pressing mechanism of the baffle includes a baffle sheath placed outside the baffle , the baffle sheath is installed on the upper surface of the bottle body, the baffle sheath is provided with a baffle jack, the baffle passes through the upper surface of the baffle sheath from the baffle jack, and the baffle sheath One end of the rubber band is fixedly connected to the edge of the surface, and a hook for fixing the free end of the rubber band is set at the edge of the upper surface of the baffle sheath away from the fixed end of the rubber band. When the rubber band is stretched, the free end of the rubber band is connected to the hook. The tendon passes through the groove provided on the top of the baffle to press the baffle toward the bottle body, the spring is compressed, and the insert is inserted into the bottom of the slot to close the through hole.

Specifically, a rubber sealing gasket is provided at the bottom of the slot, and the distance between the bottom of the through hole and the bottom surface (inner surface) of the bottle body is 1-2 mm. The design of the rubber sealing gasket makes the contact between the insert and the bottom of the slot more closely, and plays a role of sealing and isolation for replacement. During the cell culture process, the amount of culture medium in the culture bottle is relatively limited, and it is designed to be close to the bottom of the bottle by 1-2mm The through holes can ensure the mutual flow of culture medium.

Specifically, the pressure changing part capable of changing the air pressure inside the cultivation chamber includes a rubber balloon, and the suction port of the rubber balloon is connected to the vent hole provided on the side wall of the bottle body to communicate with the cultivation chamber. Compress the rubber balloon to discharge the gas inside the balloon. At this time, close the airtight cover, and then loosen the balloon body. The suction port of the balloon sucks air from the cultivation chamber, reducing the air pressure in the cultivation chamber and improving the elasticity of the bottom surface of the cultivation chamber. The film stretches and bulges under the action of negative pressure, compresses the balloon again, the gas in the ball rushes into the cultivation chamber, the air pressure in the cultivation chamber recovers, and the shape of the elastic film recovers, repeating this several times, so that the elastic film (including the edge) moves Fluctuating due to the change of the enzyme, the cells digested by transferase fall off from the elastic film. At the same time, the process of changing the air pressure in the culture chamber by the rubber balloon also causes the disturbance of the airflow in the bottle. The airflow acts on the medium, making the cells in the medium The cell suspension is fully dispersed to form a cell suspension. After the above operations are completed, the cell transfer operation can be completed by simply sucking up the cell suspension with a dropper, and the operation is simple and convenient.

Specifically, the upper surface of the bottle body, the side wall, and the rigid support plate on the bottom of the culture chamber are all transparent hard plastic structures, and the elastic film on the upper layer of the bottom of the culture chamber is a PDMS transparent elastic film, and the surrounding area of the elastic film is The edge is fixedly connected with the rigid support plate below, forming a closed space between the elastic film and the rigid support plate. The PDMS transparent elastic film has good elasticity and light transmittance, which can meet the needs of observing cells with an optical microscope during cell culture. The thickness of the elastic film is 150-300 microns. The inventors found that the PDMS film of this thickness has better elasticity, and is not easy to be damaged in the process of repeated stretching and deformation, and can be used repeatedly, which can ensure the service life of the cell culture bottle . The total thickness of the double-layer structure on the bottom of the culture chamber is 0.8-1.2 mm, which can meet the observation needs of the optical fiber microscope.

The beneficial effects of the present invention are: the present invention provides a cell co-cultivation culture bottle which is convenient for cell transfer. This kind of culture bottle can realize the exchange and exchange of cell secretions at any time according to the needs of the experiment, realize the information exchange between cells, realize the co-cultivation of cells, and can also be used when transferring cells The adherent cells at the bottom of the culture flask are easily detached from the bottom of the entire flask, which makes the experimental operation that needs to transfer cells more convenient. Specifically, the cell culture bottle divides the bottle body into two culture chambers through a spacer with a switchable communication mechanism. The two culture chambers are each provided with a bottle mouth with an airtight cover. The communication mechanism on the spacer When closed, the two culture chambers are independent of each other, and the different cells cultured in the two culture chambers do not affect each other. According to the growth requirements of the cells themselves and the experimental needs, the liquid exchange (replacement of the medium) can be completed independently through the bottle mouth of each culture chamber. Routine cell experiment operations such as passage without affecting the growth of cells in another culture room. In the operation that needs to transfer cells such as cell subculture, after completing the step of transferase digestion and adding a sufficient amount of medium, it is only necessary to repeatedly change the air pressure in the culture chamber by closing the airtight cap and sealing the bottle body by using the variable pressure part of the culture chamber. The pressure changing part reduces the air pressure in the culture chamber, and the elastic film on the bottom of the culture chamber stretches and bulges under the action of negative pressure. At the same time, the entire elastic film (including the edge) to which the cells adhere fluctuates with the change of air pressure, causing the cells digested by transferase to fall off from the elastic film. The disturbance of the airflow in the bottle, the airflow acts on the medium, so that the cells in the medium are fully dispersed to form a cell suspension. In the traditional operation, use a dropper to absorb the medium and continuously wash and blow the bottom of the culture bottle to make the cells fall off from the bottom of the bottle to obtain the cell suspension. The movement of the bottle body is greatly restricted, and it is difficult for the culture medium to re-brush to the corner position of the bottom surface of the bottle body. At the same time, when the experiment requires the communication of cells between the two culture chambers, the communication mechanism on the spacer part can be opened, and the medium in the two culture chambers can diffuse and exchange with each other through the passage of the communication mechanism, so as to realize the communication and exchange of cell secretions, thereby Realize the information exchange between cells to achieve the purpose of cell co-cultivation. If adherent cells are cultivated in both culture chambers, different cells are still in their respective culture chambers and do not mix with each other during the process of cell information exchange and interaction. , to facilitate observation and observation of cell growth and shape changes. At the same time, during the process of cell co-cultivation, close the airtight lids of the two culture chambers to seal the culture flasks, and use the pressure-changing mechanism to slightly fluctuate the elastic films on the bottom of the two culture chambers for an appropriate time. , It can also speed up the mutual mixing of culture media between culture chambers, enhance the interaction between cell secretions, and be more conducive to experimental observation.

Description of drawings

Fig. 1 is the structural representation of the separated state of two culture chambers of the cell culture bottle of the present invention;

Fig. 2 is the AA sectional view of Fig. 1;

Fig. 3 is the big picture of B prescription of Fig. 1;

Fig. 4 is the sectional view of dividing plate;

Fig. 5 is a schematic structural view of the communication state between the two culture chambers of the cell culture bottle of the present invention;

Fig. 6 is A'A' to sectional view of Fig. 5;

In the figure: 1 bottle body, 2 culture chamber, 3 airtight cover, 4 partitions, 5 inserts, 6 rubber bands; 7 grooves, 8 through holes, 9 rubber sealing gaskets, 10 hooks, 11 rubber wind balls, 12 Elastic film, 13 springs, 14 microporous membranes, 15 slots, 16 baffle sheaths, 17 baffles, 18 baffle jacks.

Detailed ways

In order to clearly illustrate the technical features of the solution, the solution will be described below through a specific implementation mode combined with the accompanying drawings.

As shown in Figures 1 to 6, a cell co-cultivation culture bottle for convenient cell transfer, the bottle body 1 of the culture bottle is divided into two culture chambers 2 by a spacer, and the side walls of each culture chamber 2 are respectively The mouth of the culture bottle is provided, and the mouth of the culture bottle is connected to the airtight cover 3 that seals the mouth of the bottle. The spacer is provided with a communication mechanism that can communicate with the two culture chambers 2. Organ, the side wall of the cultivation chamber 2 is also provided with a variable pressure component that can change the internal air pressure of the cultivation chamber 2. The bottom surface of the cultivation chamber 2 is a double-layer structure. The upper structure is an elastic film 12 located in the cultivation chamber, and the lower structure It is a rigid support plate located below the elastic film 12. Close the airtight cover 3 to seal the bottle body 2, and the pressure-transforming component of the cultivation chamber 1 causes the negative pressure in the cultivation chamber to rise, and the elastic film 12 bulges. A closed cavity is formed between them.

Specifically, the spacer is arranged in the center of the bottle body 1, and the bottle body 1 is equally divided into left and right culture chambers 2, and the mouth of each culture chamber 2 is arranged on the side wall away from the spacer. , the pressure-transforming component is arranged on the front side wall of each culture chamber 2 . The spacer includes a partition 4 that divides the interior of the bottle body 1 into two culture chambers 2, and the bottom of the partition 4 is provided with a through hole 8 that communicates with the two culture chambers 2 near the bottom surface of the bottle body 1. The spacer also includes a sealable The insertion sheet 5 of the through hole 8, the partition 4 is provided with a slot 15 matching the insertion sheet 5, the port of the slot 15 is located at the top of the partition 4, the slot 15 runs through the through hole 8, and the insertion sheet 5 is inserted into the partition 4 from the port of the slot 15, and the spacer is also provided with an insert positioning mechanism for fixing the position of the insert 5 to close or open the through hole 8, and the spacer is also provided with a function to prevent the culture chamber 2 from leaking the sealing mechanism. The port on one side of the through hole 8 is covered with a microporous filter membrane 14 with a membrane pore size of 30 microns. The insertion piece positioning mechanism includes a baffle plate 17 installed on the top of the insertion piece 5, the size of the bottom surface of the baffle plate 17 is greater than the size of the top surface of the insertion piece 5 connected thereto, and the baffle plate 17 is positioned at the outside of the bottle body 1. The upper surface of the bottle body 1 is provided with an opening corresponding to the port at the top of the slot 15, and the shape and size of the opening are the same as the port at the top of the slot 15, and the insert 5 passes through the opening of the upper surface of the bottle body 1 to connect the baffle plate 17 of the bottle body 1 , the sealing mechanism for preventing the air leakage of the cultivation chamber 2 is a rubber sealing ring installed at the opening of the upper surface of the bottle body 1, and the rubber sealing ring is closely attached to the side wall of the insert piece 5; the baffle plate 17 is set There are elastic support mechanism and pressing mechanism, and the elastic support mechanism supports the baffle 17 above the bottle body 1 in a natural state, and the insert 5 connected to the baffle 17 is limited in the slot 15 above the through hole 8, and the through hole 8 open; said compression mechanism can compress and deform the elastic support mechanism, so that the baffle 17 is close to the upper surface of the bottle body 1. Under the pressure of the compression mechanism, the baffle 17 drives the insertion piece 5 to insert the slot 15 at the bottom to close the through hole 8 . The elastic support mechanism of the baffle plate 17 is two springs 13 symmetrically arranged at the bottom of the baffle plate 17, one end of the spring 13 is connected to the bottom surface of the baffle plate 17, and the other end of the spring 13 is connected to the upper surface of the bottle body 1, and the spring 13 is not affected. When the pressure of the pressing mechanism acts, the baffle plate 17 is supported above the bottle body 1, and the insert 5 connected to the baffle plate 17 is limited in the slot 15 above the through hole 8, and the through hole 8 is opened; the baffle plate 17 The compression mechanism includes a baffle sheath 16 that is sleeved on the outside of the baffle 17, and the baffle sheath 16 is installed on the upper surface of the bottle body 1, and the baffle sheath 16 is provided with a baffle insertion hole 18, and the baffle 17 Pass through the upper surface of the baffle sheath 16 from the baffle jack 18, the edge of the upper surface of the baffle sheath 16 is fixedly connected to one end of the rubber band 6, away from the edge of the baffle sheath 16 upper surface of the fixed end of the rubber band 6 A hook 10 for fixing the free end of the rubber band 6 is set at the place. Under the stretched state of the rubber band 6, the free end of the rubber band 6 is connected to the hook 10, and the rubber band 6 passes through and is provided with a groove 7 on the top of the baffle plate 17 to the bottle body 1. The direction presses the baffle plate 17, the spring 13 is compressed, and the insertion piece 5 is inserted into the bottom of the slot 15 to close the through hole 8. The bottom of the slot 15 is provided with a rubber sealing gasket 9, and the distance between the bottom of the through hole 8 and the bottom of the bottle body 1 is 1.5 mm. The variable pressure component capable of changing the internal air pressure of the cultivation chamber 2 includes a rubber balloon 11 , and the suction port of the rubber balloon 11 is connected to the vent hole provided on the side wall of the bottle body 1 to communicate with the cultivation chamber 2 . The upper surface of the bottle body 1, the side wall and the rigid support plate on the lower floor of the culture chamber 2 are all transparent hard plastic structures, and the elastic film 12 on the upper layer of the culture chamber 2 bottom surface is a PDMS transparent elastic film, and the elastic film 12 The surrounding edges of the elastic film 12 and the rigid support plate below are fixedly connected to form a closed space between the elastic film 12 and the rigid support plate. The thickness of the elastic film 12 is 150-300 microns, and the total thickness of the double layer structure on the bottom of the culture chamber 2 is 0.8-1.2 mm.

The implementation methods listed above are only for understanding the present invention, and are not intended to limit the technical solutions described in the present invention. Those of ordinary skill in the relevant fields can also make various changes on the basis of the technical solutions described in the claims. or deformation, all equivalent changes or deformations shall fall within the protection scope of the claims of the present invention. The parts of the present invention that are not described in detail are known technologies of those skilled in the art.

Claims (8)

1. The cell co-culture bottle is characterized in that a bottle body (1) of the culture bottle is divided into two culture chambers (2) by a spacing component, a culture bottle opening is respectively arranged on the side wall of each culture chamber (2), a sealing cover (3) for sealing the bottle opening is connected with the culture bottle opening, the spacing component is provided with a communication mechanism capable of communicating the two culture chambers (2), the communication mechanism is provided with a sealing mechanism capable of separating the passages of the two culture chambers (2), the side wall of the culture chamber (2) is also provided with a pressure changing component capable of changing the internal pressure of the culture chamber (2), the bottom surface of the culture chamber (2) is of a double-layer structure, the upper-layer structure is a rigid support plate positioned below the elastic membrane (12) and is used for sealing the bottle body (1), and the elastic membrane (12) is bulged under the condition that the pressure changing component of the culture chamber (2) generates negative pressure in the culture chamber, so that a sealing cavity is formed between the elastic membrane (12) and the rigid support plate; the partition component comprises a partition board (4) for dividing the interior of the bottle body (1) into two culture chambers (2), a through hole (8) for communicating the two culture chambers (2) is formed in the lower portion of the partition board (4) close to the bottom surface of the bottle body (1), the partition component further comprises an inserting piece (5) capable of sealing the through hole (8), an inserting groove (15) matched with the inserting piece (5) is formed in the partition board (4), a port of the inserting groove (15) is formed in the top of the partition board (4), the inserting groove (15) penetrates through the through hole (8), the inserting piece (5) is inserted into the partition board (4) from the port of the inserting groove (15), and the partition component is further provided with an inserting piece positioning mechanism for fixing the position of the inserting piece (5) to seal or open the through hole (8), and the partition component is further provided with a sealing mechanism for preventing the culture chambers (2) from leaking gas; the inserting sheet positioning mechanism comprises a baffle plate (17) arranged at the top of the inserting sheet (5), the size of the bottom surface of the baffle plate (17) is larger than that of the top surface of the inserting sheet (5) connected with the baffle plate, the baffle plate (17) is positioned outside the bottle body (1), an opening is formed in the upper surface of the bottle body (1) corresponding to a port at the top of the slot (15), the shape and the size of the opening are the same as those of the port at the top of the slot (15), the inserting sheet (5) penetrates out of the opening at the upper surface of the bottle body (1) to be connected with the baffle plate (17), and the sealing mechanism for preventing the air leakage of the culture chamber (2) is a rubber sealing ring arranged at the opening at the upper surface of the bottle body (1), and the rubber sealing ring is tightly attached to the side wall of the inserting sheet (5); the baffle (17) is provided with an elastic supporting mechanism and a pressing mechanism, the elastic supporting mechanism supports the baffle (17) above the bottle body (1) in a natural state, the inserting piece (5) connected with the baffle (17) is limited in the slot (15) above the through hole (8), and the through hole (8) is opened; the pressing mechanism can press the elastic supporting mechanism to compress and deform, so that the baffle plate (17) is close to the upper surface of the bottle body (1), and under the pressure action of the pressing mechanism, the baffle plate (17) drives the inserting piece (5) to be inserted into the bottom of the inserting groove (15) to seal the through hole (8).

2. A cell co-culture flask for facilitating cell transfer according to claim 1, wherein the partition member is disposed at the center of the flask body (1) and equally divides the flask body (1) into left and right culture chambers (2), the mouth of each culture chamber (2) is disposed on a side wall away from the partition member, and the pressure changing member is disposed on a front side wall of each culture chamber (2).

3. A cell co-culture flask for facilitating cell transfer according to claim 1, wherein a microporous filter membrane (14) is covered at a side port of the through hole (8), and a membrane pore size of the microporous filter membrane is smaller than 50 μm.

4. The cell co-culture flask convenient for transferring cells according to claim 1, wherein the elastic supporting mechanism of the baffle plate (17) is two springs (13) symmetrically arranged at the bottom of the baffle plate (17), one end of each spring (13) is connected with the bottom surface of the baffle plate (17), the other end of each spring (13) is connected with the upper surface of the flask body (1), when the springs (13) are not acted by the pressing mechanism, the baffle plate (17) is supported above the flask body (1), the inserting piece (5) connected with the baffle plate (17) is limited in the slot (15) above the through hole (8), and the through hole (8) is opened; the pressing mechanism of baffle (17) is including overlapping in baffle sheath (16) of baffle (17) outside, baffle sheath (16) are installed on bottle (1) upper surface, baffle sheath (16) are provided with baffle jack (18), the upper surface of baffle sheath (16) is worn out from baffle jack (18) to baffle (17), the one end of edge fixed connection rubber band (6) of baffle sheath (16) upper surface, the edge of baffle sheath (16) upper surface of keeping away from rubber band (6) stiff end sets up couple (10) of fixed rubber band (6) free end, under rubber band (6) tensile state, couple (10) are connected to the free end of rubber band, rubber band (6) pass set up in baffle (17) top be provided with slot (7) to bottle (1) direction oppression baffle (17), spring (13) compress, insert slot (15) bottom and seal through-hole (8).

5. The cell co-culture flask convenient for transferring cells according to claim 1, wherein a rubber sealing gasket (9) is arranged at the bottom of the slot, and the distance between the bottom of the through hole (8) and the bottom surface of the flask body (1) is 1-2 mm.

6. The cell co-culture flask for facilitating cell transfer according to claim 1, wherein the pressure changing component capable of changing the internal air pressure of the culture chamber (2) comprises a rubber air ball (11), and the air suction port of the rubber air ball (11) is connected with a vent hole arranged on the side wall of the flask body (1) and communicated with the culture chamber (2).

7. The cell co-culture flask convenient for transferring cells according to claim 1, wherein the upper surface, the side wall and the rigid support plate of the lower layer of the bottom surface of the flask body (1) and the culture chamber (2) are all transparent hard plastic structures, the elastic film (12) of the upper layer of the bottom surface of the culture chamber (2) is a PDMS transparent elastic film, the peripheral edge of the elastic film (12) is fixedly connected with the rigid support plate below the elastic film, and a closed space is formed between the elastic film (12) and the rigid support plate.

8. A cell co-culture flask for facilitating the transfer of cells according to claim 7, wherein the elastic membrane (12) has a thickness of 150 to 300 μm and the total thickness of the double-layer structure of the bottom surface of the culture chamber (2) is 0.8 to 1.2 mm.

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