CN217535988U - A multi-type cell co-culture dish - Google Patents

Abstract

The utility model provides a multiple type of cell culture dish altogether, including lower floor's culture dish and upper culture dish, upper culture dish closely offsets with lower floor's culture dish inner wall, leaves the clearance between upper culture dish bottom and the lower floor's culture dish bottom, and upper culture dish bottom is equipped with a plurality of circular cones protrudingly, and the upper culture dish lateral wall is equipped with the exchange hole. Many kinds of cell coculture dishes can cultivate multiple cell simultaneously, the suspension growth environment of cell in vivo can be simulated to the upper culture dish, realizes 3D cell culture, its is rational in infrastructure, uses extensively.

Description

A multi-type cell co-culture dish

technical field

The utility model belongs to the field of experimental equipment, in particular to a multi-type cell co-culture dish.

Background technique

Petri dish is a kind of laboratory vessel for microbial culture or cell culture. It has good air permeability and pollution prevention, and has a wide range of applications in the laboratory.

At present, the co-culture of various types of cells is mainly carried out in experimental equipment such as ordinary culture dishes, multi-well plates, and transwell culture dishes, which can meet the direct or indirect communication between various types of cells. In ordinary culture dishes or multi-well plates, the cells are fully mixed, and it is more complicated to observe the changes of individual cells; and in transwell culture dishes and ordinary culture equipment, many kinds of cells grow flat, which is difficult to simulate the suspension environment in vivo.

Utility model content

In view of this, the present utility model aims to propose a multi-type cell co-culture dish, so as to realize 3D cell culture, simulate the in vivo growth environment, and at the same time facilitate observation.

In order to achieve the above object, the technical scheme of the present utility model is achieved in this way:

A multi-type cell co-culture dish, comprising a lower-layer culture dish and an upper-layer culture dish, the upper-layer culture dish and the inner wall of the lower-layer culture dish are in close contact, the upper-layer culture dish is erected in the lower-layer culture dish, and the upper-layer culture dish has an upper side wall and a lower-layer culture dish. The inner walls of the petri dish are in contact with each other, and there is a gap between the bottom of the upper petri dish and the bottom of the lower petri dish. The bottom of the upper petri dish is evenly provided with a plurality of conical protrusions, and the tips of the conical protrusions are set upward to promote the culture medium and cells there. It has a larger contact area and 3D culture function, which can better simulate the environment of cells in vivo. There are exchange holes on the side wall of the upper culture dish. The size of the exchange holes can make various cytokines, exosomes, and microvesicles. It is possible to support the exchange flow of cytokines and the like in the co-culture system by allowing the cells to pass through without passing the cells.

Further, the side wall of the upper layer culture dish is inclined, the upper end opening area of the upper layer culture dish is larger than the bottom area, and the vertical cross-sectional view of the upper layer culture dish is an inverted trapezoid, which provides more growth space as the cells are cultured.

Further, the bottom of the lower layer culture dish is connected with a liquid replenishing pipe, and the liquid replenishing pipe and the side wall of the lower layer culture dish are inclined at a certain angle, which is convenient for bottom cell planting, culturing, liquid exchange, collection and other operations.

Further, an end cap is detachably connected to the end of the liquid replenishing tube, and corresponding threads are provided between the end of the liquid replenishing tube and the end cap, and the end cap can prevent dust from entering the co-culture dish.

Further, the end cover is provided with ventilation holes to provide a gas exchange channel for the co-culture dish.

Further, an antibacterial and breathable film is provided on the end cover to prevent external bacteria from entering the co-culture dish and affect the culture effect.

Further, the lower-layer culture dish is detachably connected with a culture-dish cover, the inner diameter of the culture-dish cover is slightly larger than the outer diameter of the lower-layer culture-dish, and the culture-dish cover is buckled on the upper-layer culture-dish and the lower-layer culture-dish to play a protective role. .

Further, the co-culture dish is made of a transparent material, preferably polystyrene, so as to facilitate the observation of cell growth.

Compared with the prior art, the multi-type cell co-culture dish described in the present invention has the following advantages:

(1) The multi-type cell co-culture dish described in the present utility model can simultaneously cultivate a variety of cells, and the upper layer of the culture dish can simulate the suspended growth environment of cells in the body to realize 3D cell culture, and its structure is reasonable and widely used;

(2) The multi-type cell co-culture dish described in the present utility model can carry out operations such as planting, culturing, changing liquid, and collecting the bottom cells without opening the cover of the culture dish by setting a liquid refilling tube that communicates with the lower-layer culture dish. , easy to use.

Description of drawings

The accompanying drawings that constitute a part of the present invention are used to provide further understanding of the present invention, and the schematic embodiments and descriptions of the present invention are used to explain the present invention and do not constitute an improper limitation of the present invention. In the attached image:

FIG. 1 is a schematic structural diagram of a multi-type cell co-culture dish according to an embodiment of the present invention.

Description of reference numbers:

1. Petri dish cover; 2. Upper Petri dish; 3. Conical protrusions; 4. Lower Petri dish; 5. End cap; 6. Infusion tube.

Detailed ways

It should be noted that the embodiments of the present invention and the features of the embodiments may be combined with each other under the condition of no conflict.

In the description of the present invention, it should be understood that the terms "center", "longitudinal", "horizontal", "upper", "lower", "front", "rear", "left", "right", The orientations or positional relationships indicated by "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility. The novel and simplified description does not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operate in a particular orientation, and therefore should not be construed as limiting the invention. In addition, the terms "first", "second", etc. are used for descriptive purposes only, and should not be construed as indicating or implying relative importance or implying the number of indicated technical features. Thus, a feature defined as "first", "second", etc., may expressly or implicitly include one or more of that feature. In the description of the present invention, unless otherwise specified, "plurality" means two or more.

In the description of the present invention, it should be noted that, unless otherwise expressly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it may be a fixed connection or a connectable connection. Detachable connection, or integral connection; may be mechanical connection or electrical connection; may be direct connection, or indirect connection through an intermediate medium, or internal communication between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood through specific situations.

The present utility model will be described in detail below with reference to the accompanying drawings and in conjunction with the embodiments.

In the multi-type cell co-culture dish in the present invention, the co-culture dish is made of transparent material, preferably polystyrene, so as to facilitate the observation of cell growth. The co-culture dish includes a lower-layer culture dish and an upper-layer culture dish. The inner wall of the petri dish is closely abutted, the upper petri dish is erected in the lower petri dish, the upper end side wall of the upper petri dish is in contact with the inner wall of the lower petri dish, and there is a gap between the bottom of the upper petri dish and the bottom of the lower petri dish,

The side wall of the upper layer of culture dish is inclined, the upper opening area of the upper layer of culture dish is larger than the bottom area, and the vertical cross-sectional view of the upper layer of culture dish is an inverted trapezoid, which provides more growth space as the cells are cultured.

The bottom of the upper petri dish is evenly equipped with a plurality of conical protrusions, and the tips of the conical protrusions are set upward, which promotes a larger contact area between the medium and the cells, and has a 3D culture function, which better simulates the environment in which the cells are located in the body. The size of the exchange hole allows various cytokines, exosomes, microvesicles, etc. to pass through without allowing cells to pass through, which can support the exchange and flow of cytokines in the co-culture system.

The bottom of the lower petri dish is connected with a rehydration tube, and the rehydration tube and the side wall of the lower petri dish are inclined at a certain angle, which is convenient for the bottom cell planting, culture, liquid exchange, collection and other operations. There are corresponding threads between the end of the tube and the end cap. The end cap can prevent dust from entering the co-culture dish. The end cap is provided with ventilation holes to provide a gas exchange channel for the co-culture dish. The end cap is provided with antibacterial ventilation. Membrane to prevent external bacteria from entering the co-culture dish, affecting the culture effect,

A petri dish cover is detachably connected to the lower petri dish, the inner diameter of the petri dish cover is slightly larger than the outer diameter of the lower petri dish, and the petri dish cover is buckled on the upper petri dish and the lower petri dish to play a protective role.

The above are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the within the protection scope of the present invention.

Claims (8)

1. a multi-type cell co-culture dish is characterized in that: comprise lower-layer culture dish and upper-layer culture-dish, described upper-layer culture-dish and lower-layer culture-dish inner wall are closely abutted, and between upper-layer culture-dish bottom and lower-layer culture-dish bottom, there is a A plurality of conical protrusions are arranged at the bottom of the upper culture dish, and exchange holes are arranged on the side walls of the upper culture dish. 2 . The multi-type cell co-culture dish according to claim 1 , wherein the side wall of the upper layer culture dish is inclined, and the opening area of the upper end of the upper layer culture dish is larger than the bottom area. 3 . 3 . The multi-type cell co-culture dish according to claim 1 , wherein the bottom of the lower layer culture dish is connected with a fluid supplement pipe. 4 . 4 . The multi-type cell co-culture dish according to claim 3 , wherein an end cap is detachably connected to the end of the fluid supplement pipe. 5 . 5 . The multi-type cell co-culture dish according to claim 4 , wherein the end cap is provided with ventilation holes. 6 . 6 . The multi-type cell co-culture dish according to claim 5 , wherein the end cap is provided with an antibacterial and breathable membrane. 7 . 7 . The multi-type cell co-culture dish according to claim 1 , wherein the lower layer culture dish is detachably connected with a culture dish cover. 8 . 8 . The multi-type cell co-culture dish according to claim 1 , wherein the co-culture dish is made of transparent material. 9 .

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