CN105886380B - A kind of Petri dish box - Google Patents
A kind of Petri dish box Download PDFInfo
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- CN105886380B CN105886380B CN201610226916.8A CN201610226916A CN105886380B CN 105886380 B CN105886380 B CN 105886380B CN 201610226916 A CN201610226916 A CN 201610226916A CN 105886380 B CN105886380 B CN 105886380B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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Abstract
本发明涉及生物实验仪器设备领域,特别涉及一种培养皿盒。本发明提供一种培养皿盒,包括盒盖,底座和培养皿架,所述盒盖呈圆柱形,内中空,顶端封闭,侧壁内表面下端设置有螺纹,侧壁中间设置有通气开关;所述底座是实心圆柱体,侧表面上端设置有螺纹,顶端设置有插孔,插孔不贯穿整个底座,为底座高度的3/4,所述底座侧表面上端螺纹与盒盖侧壁内表面下端螺纹相对应,盒盖通过螺纹开闭;所述培养皿架由支柱和一个以上托盘组成,支柱和托盘是一体式结构,无法拆卸,所述支柱与底座插孔嵌插式对应,培养皿架通过支柱固定在底座上,可拆卸。本发明所述的培养皿盒不仅为培养皿灭菌、培养基平板凝固、特殊微生物培养提供了私有空间,而且还可以降低微生物接种培养整个过程的染菌率。
The invention relates to the field of biological experiment equipment, in particular to a petri dish box. The invention provides a petri dish box, comprising a box cover, a base and a petri dish rack, the box cover is cylindrical, hollow inside, closed at the top, threads are provided at the lower end of the inner surface of the side wall, and a ventilation switch is provided in the middle of the side wall; The base is a solid cylinder, the upper end of the side surface is provided with threads, and the top is provided with a jack, the jack does not penetrate the entire base, which is 3/4 of the height of the base, the upper end of the side surface of the base is threaded and the inner surface of the side wall of the box cover Corresponding to the thread at the lower end, the box cover is opened and closed through the thread; the petri dish rack is composed of a pillar and more than one tray. The frame is fixed on the base by pillars and can be disassembled. The culture dish box of the present invention not only provides a private space for the sterilization of the culture dish, the solidification of the medium plate, and the cultivation of special microorganisms, but also can reduce the bacterial contamination rate in the whole process of microorganism inoculation and cultivation.
Description
技术领域technical field
本发明涉及生物实验仪器设备领域,特别涉及一种培养皿盒。The invention relates to the field of biological experiment equipment, in particular to a petri dish box.
背景技术Background technique
培养皿是微生物实验室经常用到的玻璃实验器材,主要用来培养各种微生物。通常情况下微生物接种培养的过程如下:(1)洗净晾干的培养皿用报纸包裹,高压锅灭菌;(2)超净工作台中制作培养基平板,待凝固(因为实验室超净工作台数量有限,所以平板凝固过程中其他人可以任意使用);(3)接种;(4)把接种过的培养皿装进一次性保鲜袋中扎口;(5)转移到培养箱中培养(因为实验室培养箱数量有限,所以各种微生物放在一起培养,而且培养期间其他人可以任意打开培养箱)。以上整个操作过程存在明显缺陷:(1)培养皿灭菌没有固定的容器,只能用报纸包裹,不仅费时费力而且灭菌过程中容易进水造成培养皿散开灭菌不彻底;(2)倒好的培养基平板凝固过程中容易染菌;(3)一次性保鲜袋包扎,不仅浪费而且不透气,不利于好氧菌的生长;(4)培养箱中各种微生物放在一起培养不仅容易染菌,而且好氧厌氧条件不能实现。Petri dishes are glass experimental equipment often used in microbiology laboratories, mainly used to cultivate various microorganisms. Usually, the process of microbial inoculation and cultivation is as follows: (1) wash and dry the petri dish, wrap it in newspaper, and sterilize it in a pressure cooker; The quantity is limited, so other people can use it at will during the solidification process of the plate); (3) inoculate; (4) put the inoculated culture dish into a disposable fresh-keeping bag; (5) transfer it to the incubator for cultivation (because The number of laboratory incubators is limited, so various microorganisms are cultivated together, and others can open the incubator arbitrarily during the cultivation period). There are obvious defects in the above whole operation process: (1) There is no fixed container for the sterilization of the petri dish, and it can only be wrapped in newspaper, which is not only time-consuming and laborious, but also easy to enter water during the sterilization process, causing the petri dish to scatter and the sterilization is not complete; (2) It is easy to be infected with bacteria during the solidification process of the poured medium plate; (3) the disposable fresh-keeping bag is not only wasteful but also airtight, which is not conducive to the growth of aerobic bacteria; (4) it is not only easy to cultivate various microorganisms in the incubator together Infection, and aerobic anaerobic conditions can not be achieved.
发明内容Contents of the invention
鉴于以上所述现有技术的缺点,本发明的目的在于提供一种培养皿盒,用于解决现有技术中的问题。In view of the above-mentioned shortcomings of the prior art, the object of the present invention is to provide a petri dish box for solving the problems in the prior art.
为实现上述目的及其他相关目的,本发明提供一种培养皿盒,包括盒盖,底座和培养皿架,所述盒盖呈圆柱形,内中空,顶端封闭,侧壁内表面下端设置有螺纹,侧壁中间设置有通气开关,所述通气开关有两个,左右对称分布;所述底座是实心圆柱体,侧表面上端设置有螺纹,顶端设置有插孔,插孔不贯穿整个底座,深度为底座高度的3/4,所述插孔有三个,其中两个插孔左右对称分布,第三个插孔分布在前两个插孔连接形成的圆弧中间,所述底座侧表面上端螺纹与盒盖侧壁内表面下端螺纹相对应,盒盖通过螺纹开闭;所述培养皿架由支柱和一个以上托盘组成,支柱和托盘是一体式结构,无法拆卸,所述支柱有三个,分布在托盘边缘,下端突出于托盘,上端不突出,其中第一支柱和第二支柱在托盘左右两侧对称分布,第三支柱分布在第一支柱、第二支柱之间,所述支柱与底座插孔嵌插式对应,培养皿架通过支柱固定在底座上,可拆卸,所述托盘有裙边,边缘有个30度扇形缺口,与第三支柱呈中心对称。In order to achieve the above object and other related objects, the present invention provides a petri dish box, comprising a lid, a base and a petri dish rack, the lid is cylindrical, hollow inside, closed at the top, and threaded at the lower end of the inner surface of the side wall , there is a ventilation switch in the middle of the side wall, and there are two ventilation switches, symmetrically distributed left and right; the base is a solid cylinder, the upper end of the side surface is provided with threads, and the top is provided with a jack, the jack does not penetrate the entire base, the depth It is 3/4 of the height of the base. There are three jacks, two of which are distributed symmetrically on the left and right, and the third jack is distributed in the middle of the arc formed by the connection of the first two jacks. The upper end of the side surface of the base is threaded. Corresponding to the thread on the lower end of the inner surface of the side wall of the box cover, the box cover is opened and closed through the thread; the culture dish rack is composed of a pillar and more than one tray. The pillar and the tray are an integral structure and cannot be disassembled. There are three pillars distributed On the edge of the tray, the lower end protrudes from the tray, and the upper end does not protrude, wherein the first pillar and the second pillar are symmetrically distributed on the left and right sides of the tray, and the third pillar is distributed between the first pillar and the second pillar, and the pillar is inserted into the base Corresponding to the hole insertion type, the petri dish rack is fixed on the base through a pillar and is detachable. The tray has a skirt and a 30-degree fan-shaped gap on the edge, which is centrally symmetrical with the third pillar.
优选的,所述通气开关由插板、插槽和通气孔组成。Preferably, the ventilation switch is composed of a plugboard, a slot and a ventilation hole.
更优选的,所述插板和插槽相配合。More preferably, the plug-in board matches the slot.
更有选的,所述插板上端中间位置开有一个凹槽。More optionally, a groove is formed at the middle position of the upper end of the plug-in board.
更有选的,所述通气孔开在盒盖侧壁上,对应通气孔位置的壁内侧粘附一层孔径为0.2微米的超滤膜。More preferably, the vent hole is opened on the side wall of the box cover, and a layer of ultrafiltration membrane with a pore size of 0.2 microns is adhered to the inner side of the wall corresponding to the position of the vent hole.
优选的,所述底座高度≤盒盖高度。Preferably, the height of the base ≤ the height of the lid.
优选的,培养皿盒为圆柱体,其底面直径≥其高度,底面直径>托盘直径。Preferably, the petri dish box is a cylinder, the diameter of its bottom surface ≥ its height, and the diameter of the bottom surface > the diameter of the tray.
优选的,所述托盘直径为5cm-17cm,裙边高度为1cm,托盘间间隔为4cm。Preferably, the diameter of the tray is 5cm-17cm, the height of the skirt is 1cm, and the interval between trays is 4cm.
优选的,培养皿盒各组件,包括盒盖、底座和培养皿架均采用耐高温材料制成。Preferably, each component of the petri dish box, including the lid, the base and the petri dish rack, is made of high temperature resistant materials.
本发明提供了一种培养皿盒,所述培养皿盒既可以用于微生物接种培养的整个过程,也可以单独用作培养皿灭菌容器,培养基平板凝固容器或者微生物隔离培养容器,降低染菌率。总体来说,本发明具有以下有益效果:The invention provides a petri dish box, which can be used in the whole process of microorganism inoculation and culture, and can also be used alone as a petri dish sterilization container, a medium plate solidification container or a microbial isolation culture container, reducing contamination. bacteria rate. In general, the present invention has the following beneficial effects:
(1)本发明提供的培养皿盒,用作培养皿灭菌容器可节约人力物力,易放易取,灭菌彻底;(1) The petri dish box provided by the present invention can be used as a petri dish sterilization container, which can save manpower and material resources, is easy to put and take, and can be sterilized thoroughly;
(2)本发明提供的培养皿盒,用作培养基平板凝固容器时,倒好的平板放在里面可放在任意位置等待凝固,既不占用超净工作台空间还可以降低染菌的可能性;(2) When the petri dish box provided by the present invention is used as a medium plate solidification container, the poured plate can be placed in any position to wait for solidification, which does not occupy the space of the ultra-clean workbench and can also reduce the possibility of bacterial contamination ;
(3)本发明提供的培养皿盒,用作微生物隔离培养容器时,微生物放在大培养箱中培养时可根据需要调节厌氧好氧条件并且降低污染其他杂菌的可能性;(3) When the culture dish box provided by the present invention is used as a microbial isolation culture container, when the microorganisms are cultivated in a large incubator, the anaerobic and aerobic conditions can be adjusted as required and the possibility of contaminating other miscellaneous bacteria can be reduced;
(4)本发明提供的培养皿盒,用于微生物接种培养的整个过程时,操作方便快捷,而且培养皿始终处在无菌环境中,大大降低染菌风险,提高实验成功率。(4) When the petri dish box provided by the present invention is used in the whole process of microbial inoculation and cultivation, the operation is convenient and quick, and the petri dish is always in a sterile environment, which greatly reduces the risk of bacterial contamination and improves the success rate of experiments.
附图说明Description of drawings
图1显示为本发明培养皿盒的整体结构示意图。Fig. 1 is a schematic diagram showing the overall structure of the culture dish box of the present invention.
图2显示为本发明培养皿盒通气开关关闭时的结构示意图。Fig. 2 is a schematic diagram showing the structure of the culture dish box of the present invention when the ventilation switch is turned off.
图3显示为本发明培养皿盒通气开关开放时的结构示意图。Fig. 3 is a schematic diagram showing the structure of the culture dish box of the present invention when the ventilation switch is opened.
元件标号说明Component designation description
1 盒盖1 lid
2 底座2 base
3 培养皿架3 petri dish racks
4 通气开关4 Vent switch
41 插板41 board
42 插槽42 slots
43 通气孔43 Vents
44 超滤膜44 ultrafiltration membrane
45 凹槽45 grooves
5 底座螺纹5 base thread
51 第一插孔51 First jack
52 第二插孔52 Second jack
53 第三插孔53 Third jack
61 第一支柱61 First Pillar
62 第二支柱62 Second Pillar
63 第三支柱63 The third pillar
7 托盘7 trays
具体实施方式Detailed ways
以下由特定的具体实施例说明本发明的实施方式,熟悉此技术的人士可由本说明书所揭露的内容轻易地了解本发明的其他优点及功效。The implementation of the present invention will be illustrated by specific specific examples below, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.
请参阅图1至图3。须知,本说明书所附图式所绘示的结构、比例、大小等,均仅用以配合说明书所揭示的内容,以供熟悉此技术的人士了解与阅读,并非用以限定本发明可实施的限定条件,故不具技术上的实质意义,任何结构的修饰、比例关系的改变或大小的调整,在不影响本发明所能产生的功效及所能达成的目的下,均应仍落在本发明所揭示的技术内容得能涵盖的范围内。同时,本说明书中所引用的如“上”、“下”、“左”、“右”、“中间”及“一”等的用语,亦仅为便于叙述的明了,而非用以限定本发明可实施的范围,其相对关系的改变或调整,在无实质变更技术内容下,当亦视为本发明可实施的范畴。See Figures 1 through 3. It should be noted that the structures, proportions, sizes, etc. shown in the drawings attached to this specification are only used to match the content disclosed in the specification, for those who are familiar with this technology to understand and read, and are not used to limit the implementation of the present invention. Limiting conditions, so there is no technical substantive meaning, any modification of structure, change of proportional relationship or adjustment of size, without affecting the effect and purpose of the present invention, should still fall within the scope of the present invention. The disclosed technical content must be within the scope covered. At the same time, terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description and are not used to limit this specification. The practicable scope of the invention and the change or adjustment of its relative relationship shall also be regarded as the practicable scope of the present invention without any substantial change in the technical content.
如图1所示,本发明提供一种培养皿盒,包括盒盖(1),底座(2)和培养皿架(3),底座(2)高度≤盒盖(1)高度,培养皿盒为圆柱体,其底面直径≥其高度,以保证培养皿架(3)上放置培养皿时整个培养皿盒稳定不倾倒。盒盖(1)呈圆柱形,内中空,顶端封闭,侧壁内表面下端设置有螺纹,侧壁中间设置有两个通气开关(4),左右对称分布;底座(2)是实心圆柱体,侧表面上端设置有螺纹,顶端设置有三个插孔(5),插孔(5)不贯穿整个底座(2),深度为底座(2)高度的3/4,其中第一插孔(51)和第二插孔(52)左右对称分布,第三个插孔(53)分布在前第一插孔(51)和第二插孔(52)连接形成的圆弧中间,底座(2)侧表面上端螺纹与盒盖(1)侧壁内表面下端螺纹相对应,盒盖(1)通过螺纹控制开闭;培养皿架(3)由支柱(6)和一个以上托盘(7)组成,支柱(6)和托盘(7)是一体式结构,无法拆卸。支柱(6)有三个,分布在托盘(7)边缘,下端突出于托盘(7),上端不突出;其中第一支柱(61)和第二支柱(62)在托盘左右两侧对称分布,第三支柱(63)分布在第一支柱(61)、第二支柱(62)之间,利于培养皿的取放;支柱(6)与底座插孔(5)嵌插式对应,将培养皿架(3)固定在底座(2)上,支柱(6)和底座(2)之间可拆卸,利于培养皿盒各部分的清洗。托盘(7)直径为5cm-17cm,与市售培养皿直径相对应;托盘(7)有裙边,裙边高度为1cm,防止放好的培养皿掉落;边缘有个30度扇形缺口,与第三支柱(63)呈中心对称,便于培养皿的取放;当托盘(7)数量大于1时,两个托盘(7)间间隔为4cm。As shown in Figure 1, the present invention provides a kind of petri dish box, comprises lid (1), base (2) and petri dish frame (3), base (2) height≤height of lid (1), petri dish box It is a cylinder, and its bottom surface diameter is greater than or equal to its height, so that when the culture dish is placed on the culture dish rack (3), the whole culture dish box is stable and does not topple over. The box cover (1) is cylindrical, hollow inside, closed at the top, threads are provided at the lower end of the inner surface of the side wall, and two ventilation switches (4) are arranged in the middle of the side wall, symmetrically distributed from left to right; the base (2) is a solid cylinder, Threads are provided on the upper end of the side surface, and three sockets (5) are provided on the top, the sockets (5) do not penetrate the entire base (2), and the depth is 3/4 of the height of the base (2), wherein the first socket (51) It is distributed symmetrically with the second socket (52), and the third socket (53) is distributed in the middle of the arc formed by the connection between the first socket (51) and the second socket (52). On the side of the base (2) The thread at the upper end of the surface corresponds to the thread at the lower end of the inner surface of the side wall of the box cover (1), and the opening and closing of the box cover (1) is controlled by the thread; the culture dish rack (3) is composed of a pillar (6) and more than one tray (7). (6) and pallet (7) are integral structures, can't dismantle. There are three pillars (6), distributed on the edge of the tray (7), the lower end protrudes from the tray (7), and the upper end does not protrude; wherein the first pillar (61) and the second pillar (62) are symmetrically distributed on the left and right sides of the tray, and the Three pillars (63) are distributed between the first pillar (61) and the second pillar (62), which is beneficial to the picking and placing of the culture dish; (3) It is fixed on the base (2), and the space between the pillar (6) and the base (2) is detachable, which is beneficial to the cleaning of each part of the petri dish box. The diameter of the tray (7) is 5cm-17cm, which corresponds to the diameter of the commercially available petri dish; the tray (7) has a skirt with a height of 1cm to prevent the placed petri dish from falling; there is a 30-degree fan-shaped gap on the edge, It is centrally symmetrical with the third pillar (63), which is convenient for taking and placing the culture dish; when the number of trays (7) is greater than 1, the interval between two trays (7) is 4cm.
如图2,图3所示,盒盖(1)上的通气开关(4)由插板(41)、插槽(42)和通气孔(43)组成;插板(41)和插槽(42)相配合;插板(41)上端中间位置开有一个凹槽(45),便于使用者推拉插板(41),使其沿插槽(42)滑动。As Fig. 2, shown in Fig. 3, the ventilation switch (4) on the lid (1) is made up of flashboard (41), slot (42) and ventilation hole (43); Flashboard (41) and slot ( 42) cooperate; the middle position of the upper end of the board (41) has a groove (45), which is convenient for the user to push and pull the board (41) to slide along the slot (42).
如图3所示,通气孔(43)开在盒盖(1)侧壁上,对应通气孔(43)位置的壁内侧粘附一层孔径为0.2微米的超滤膜(44)。使用时,如果需要通气或者创造供氧环境,将插板(41)拉开,露出通气孔(43),通气孔(43)内侧的超滤膜(44)可阻止细菌等杂菌进入,保证盒内的无菌环境;如果不需要通气或者创造厌氧环境,将插板(41)拉合,封闭通气孔(43),保证盒内无氧无菌环境。As shown in Figure 3, air hole (43) is opened on the lid (1) side wall, and the wall inboard of corresponding air hole (43) position is adhered one deck aperture and is the ultrafiltration membrane (44) of 0.2 micron. During use, if it is necessary to ventilate or create an oxygen supply environment, the inserting plate (41) is pulled away to expose the vent hole (43), and the ultrafiltration membrane (44) inside the vent hole (43) can prevent bacteria and other bacteria from entering, ensuring Aseptic environment in the box; if ventilation is not required or an anaerobic environment is created, the inserting plate (41) is pulled together to seal the air hole (43) to ensure an anaerobic and sterile environment in the box.
综上所述,本发明有效克服了现有技术中的种种缺点而具高度产业利用价值。To sum up, the present invention effectively overcomes various shortcomings in the prior art and has high industrial application value.
上述实施例仅例示性说明本发明的原理及其功效,而非用于限制本发明。任何熟悉此技术的人士皆可在不违背本发明的精神及范畴下,对上述实施例进行修饰或改变。因此,举凡所属技术领域中具有通常知识者在未脱离本发明所揭示的精神与技术思想下所完成的一切等效修饰或改变,仍应由本发明的权利要求所涵盖。The above-mentioned embodiments only illustrate the principles and effects of the present invention, but are not intended to limit the present invention. Anyone skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or changes made by those skilled in the art without departing from the spirit and technical ideas disclosed in the present invention should still be covered by the claims of the present invention.
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| CN106620777A (en) * | 2016-12-21 | 2017-05-10 | 明博医药技术开发(上海)有限公司 | Centrifuge tube sterilization box |
| CN107318505B (en) * | 2017-07-14 | 2023-09-26 | 遵义师范学院 | A vegetable petri dish |
| CN112961761A (en) * | 2021-02-08 | 2021-06-15 | 滨州职业学院 | Medical microbiology experimental apparatus |
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| US5395006A (en) * | 1993-04-29 | 1995-03-07 | Verma; Kuldeep | Fermentation vessels and closures therefor |
| US5520302A (en) * | 1994-01-27 | 1996-05-28 | Anderson; Roger | Petri dish having two-position lid |
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