CN204224588U - A kind of filamentous fungus purifying vessel and filamentous fungus culture dish - Google Patents
A kind of filamentous fungus purifying vessel and filamentous fungus culture dish Download PDFInfo
- Publication number
- CN204224588U CN204224588U CN201420674148.9U CN201420674148U CN204224588U CN 204224588 U CN204224588 U CN 204224588U CN 201420674148 U CN201420674148 U CN 201420674148U CN 204224588 U CN204224588 U CN 204224588U
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- hollow tubular
- vessel
- tubular vessel
- filamentous fungus
- culture dish
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Abstract
The utility model relates to a kind of filamentous fungus purifying vessel and filamentous fungus culture dish.Involved filamentous fungus purifying vessel comprise hollow tubular vessel; Involved filamentous fungus culture dish comprises ware lid and ware body, and the roof in described ware lid is connected with hollow tubular vessel, and these hollow tubular vessel extend but not bottom contact plate body, the sidewall of described hollow tubular vessel offer through hole bottom ware body.Culture dish internal space is divided into two parts by hollow tubular vessel of the present utility model, the media surface of bacterial growth in hollow tubular vessel, near vaccination, fungi can grow into media surface outside hollow tubular vessel, growth fungi of media surface outside hollow tubular vessel is carried out flash liberation again, the fungi of purifying can be obtained.This vessel can effectively be separated fungi and bacterium, short form test step, shorten purification time.
Description
Technical field
The utility model relates to spawn culture tools for purification, is specifically related to a kind of filamentous fungus purifying instrument and filamentous fungus culture dish.
Background technology
Culture dish is the most frequently used instrument that microorganism separation, cultivation, purifying are carried out in laboratory.Conventional culture dish is made up of ware body and ware lid, and structure is simple, not only for the culture of isolated of laboratory microorganism, is also usually used in the separation and purification of producing bacterial classification.Such as in edible fungus culturing practice, the bacterial classification performance of edible mushrooms usually there will be the phenomenon of production performance decline, needs its separation and purification to recover its premium properties.Utilize traditional culture dish separation and purification filamentous fungus, effect is not often very desirable.Be separated in the process of filamentous fungus in laboratory, because sample is easily contaminated by bacterial, the purifying that needs repeatedly to transfer obtains targeted fungal.In the experimentation of reality, in order to obtain the filamentous fungus of purifying, often give in substratum and add microbiotic, but this method is with the problem such as the pollution of microbiotic medicament, thalline resistance, cost intensive.
Therefore, using Nostoc commune Vanch ware when being separated fungi, for avoiding bacterial contamination, needing strict experimental implementation process and longer separation and purification time.
Summary of the invention
For defect or the deficiency of prior art, the utility model provides a kind of filamentous fungus purifying vessel, and these vessel comprise hollow tubular vessel.
Optionally, described hollow tubular vessel are round shape.
The utility model additionally provides a kind of filamentous fungus culture dish, comprise ware lid and ware body, roof in described ware lid is connected with hollow tubular vessel, and these hollow tubular vessel extend bottom ware body but not bottom contact plate body, the sidewall of described hollow tubular vessel offer through hole.
Optionally, the sidewall of described hollow tubular vessel offers multiple through hole.
Optionally, described hollow tubular vessel are positioned at the central position of ware lid.
Culture dish internal space is divided into two parts by hollow tubular vessel of the present utility model, the media surface of bacterial growth in hollow tubular vessel, near vaccination, fungi can grow into media surface outside hollow tubular vessel, growth fungi of media surface outside hollow tubular vessel is carried out flash liberation again, the fungi of purifying can be obtained.This vessel can effectively be separated fungi and bacterium, short form test step, shorten purification time.
Accompanying drawing explanation
Fig. 1 is the structural representation of vessel of the present utility model;
Fig. 2 is the use shape body structure schematic diagram of vessel of the present utility model;
Fig. 3 is the structural representation of culture dish of the present utility model;
Fig. 4 is the ware lid structural representation of culture dish of the present utility model;
Fig. 5 is the ware body structure schematic diagram of culture dish of the present utility model.
Embodiment
For simplifying the purifying process of filamentous fungus, contriver invent a kind of be placed in existing culture dish vessel, as depicted in figs. 1 and 2, the structure of designed vessel 01 is hollow tubular structure, when utilizing these vessel and general culture dish 011 pair of filamentous fungus to carry out purifying, the vessel of hollow tube-like structure being placed on inoculation has on the substratum of bacterial classification, cover ware lid, and between hollow tubular vessel and culture dish lid top wall, leave the gap of about 1 mm wide, ensure that filamentous fungus is when growing, mycelia is protruding by this gap, bacterium is limited in its interior region by hollow tubular vessel and grows, the mycelia stretched out can not be polluted, thus reach the object of purifying.
Invention based on above-mentioned hollow tubular vessel designs, contriver have also been devised a kind of culture dish, itself and general culture dish structure unlike, as shown in Figure 3, hollow tubular vessel 3 are connected with in the roof of ware lid 1, these hollow tubular vessel 3 extend to the bottom of ware body 2 but do not contact bottom (when having substratum, stretch into substratum inside), the sidewall of hollow tubular vessel 3 offers through hole 5, hypha,hyphae can stretch out from this through hole 5, when filling substratum 4 in ware body, hollow tubular vessel 5 stretch into or insert in substratum 4 simultaneously, ensure miscellaneous bacteria indiffusion growth.
Embodiment:
As in Figure 3-5, the culture dish of this embodiment comprises ware body 2 and ware lid 1, is connected with hollow tubular vessel 3 in ware lid 1 pushes up.In figure, each spot size is:
N＝4mm、D＝94mm、C＝91mm、B＝17.5mm、A＝16mm、G＝4mm、H＝5mm、I＝14mm、E＝15mm、F＝18mm、K＝19.5mm、J＝18mm、L＝87mm、M＝90m。
Culture dish wall thickness 1.5mm, ware body bottom inner diameter is 87mm, ware body inner height is 18mm, and ware lid internal diameter is 91mm, and ware lid inner height is 16mm, hollow tubular vessel 3 internal diameter 15mm, external diameter 18mm, length is 14mm, and six height that are circumferentially provided with of distance hollow tubular vessel 3 end 4mm are the through hole of 1mm, six through holes are positioned at circumferentially same and are uniformly distributed, and namely the corresponding central angle of adjacent through-holes equals 30 °.
When laboratory separation and purification filamentous fungus, first prepare fungi culture medium and sterilizing by standard operating procedure, at aseptic operating platform pour plate, substratum thickness about 4 ~ 5mm.After substratum is got well, hollow tubular vessel 3 embed in substratum, and culture dish is divided into two separate space by hollow tubular vessel 3: hollow tubular vessel 3 are inner outside with it.
During inoculation, sample is inoculated in the inner corresponding media surface of hollow tubular vessel 3, cultivate in optimal temperature, bacterium cannot cross hollow tubular vessel 3, can only be grown on the vertical corresponding media surface of hollow tubular vessel 3, hypha,hyphae can pass through hole, is grown on the substratum of hollow tubular vessel 3 outside, when being further purified, only need from a little mycelia of hollow tubular vessel 3 External Medium surface picking.The setting of culture dish hollow tubular vessel 3, makes bacterium effectively be separated with fungi, simplifies testing sequence, shorten purification time.
Claims (5)
1. filamentous fungus purifying vessel, is characterized in that, these vessel comprise hollow tubular vessel.
2. filamentous fungus purifying vessel as claimed in claim 1, it is characterized in that, described hollow tubular vessel are round shape.
3. a filamentous fungus culture dish, comprise ware lid and ware body, it is characterized in that, the roof in described ware lid is connected with hollow tubular vessel, and these hollow tubular vessel extend but not bottom contact plate body, the sidewall of described hollow tubular vessel offer through hole bottom ware body.
4. filamentous fungus culture dish as claimed in claim 3, is characterized in that, the sidewall of described hollow tubular vessel offers multiple through hole.
5. filamentous fungus culture dish as claimed in claim 3, it is characterized in that, described hollow tubular vessel are positioned at the central position of ware lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420674148.9U CN204224588U (en) | 2014-11-12 | 2014-11-12 | A kind of filamentous fungus purifying vessel and filamentous fungus culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420674148.9U CN204224588U (en) | 2014-11-12 | 2014-11-12 | A kind of filamentous fungus purifying vessel and filamentous fungus culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204224588U true CN204224588U (en) | 2015-03-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420674148.9U Expired - Fee Related CN204224588U (en) | 2014-11-12 | 2014-11-12 | A kind of filamentous fungus purifying vessel and filamentous fungus culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204224588U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110029042A (en) * | 2019-03-20 | 2019-07-19 | 上海市农业科学院 | A kind of ganoderma lucidum mycelium solid fermentation box |
| CN110551642A (en) * | 2019-09-29 | 2019-12-10 | 郑萍 | separation and purification inspection method for fungi with high pollution background |
| CN110551620A (en) * | 2019-09-29 | 2019-12-10 | 陈健 | Special culture dish device of filamentous fungi separation and purification |
-
2014
- 2014-11-12 CN CN201420674148.9U patent/CN204224588U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110029042A (en) * | 2019-03-20 | 2019-07-19 | 上海市农业科学院 | A kind of ganoderma lucidum mycelium solid fermentation box |
| CN110551642A (en) * | 2019-09-29 | 2019-12-10 | 郑萍 | separation and purification inspection method for fungi with high pollution background |
| CN110551620A (en) * | 2019-09-29 | 2019-12-10 | 陈健 | Special culture dish device of filamentous fungi separation and purification |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150325 Termination date: 20161112 |
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| CF01 | Termination of patent right due to non-payment of annual fee |