CN206266633U - A kind of cell Indirect co-culture support - Google Patents

A kind of cell Indirect co-culture support Download PDF

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Publication number
CN206266633U
CN206266633U CN201621357418.9U CN201621357418U CN206266633U CN 206266633 U CN206266633 U CN 206266633U CN 201621357418 U CN201621357418 U CN 201621357418U CN 206266633 U CN206266633 U CN 206266633U
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CN
China
Prior art keywords
donut
culture
cell
support
indirect
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201621357418.9U
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Chinese (zh)
Inventor
李丹丹
赵蒲
王占友
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Northeastern University China
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Northeastern University China
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Northeastern University China filed Critical Northeastern University China
Priority to CN201621357418.9U priority Critical patent/CN206266633U/en
Application granted granted Critical
Publication of CN206266633U publication Critical patent/CN206266633U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model provides a kind of cell Indirect co-culture support, including base and support bar, the base is formed by outer donut and the docking of interior donut, the thickness of the outer donut is more than the interior donut, and the bottom of two donuts flushes, the joint of the outer donut and the interior donut is provided with groove.The utility model simple structure, enormously simplify cell Indirect co-culture process, save experimental period, and make the low raw-material cost that the utility model support is used.Additionally, also reusable.

Description

A kind of cell Indirect co-culture support
Technical field
The utility model is related to a kind of cell culturing bracket, and in particular to a kind of cell Indirect co-culture support.
Background technology
At present, the co-culture method between two kinds of cells mainly has two kinds of Co-culture and Indirect co-culture.Wherein, It is that two or more cell is inoculated on different carriers respectively to connect co-cultivation, is then placed in together both carriers Among one culture environment, different types of cell is set to share same cultivating system and be not directly contacted with.
The conventional method of cell Indirect co-culture co-cultures method for " creep plate formula "." creep plate formula " co-cultivation method is by cell First it is inoculated with slide after treatment, after after cell attachment, is put into the culture dish of another cell with certain proportion and is total to it Culture, but the method needs first to drip wax on slide and plant cell again when operating, while also needing to six orifice plates, the burnt training of 35mm copolymerization Ware and candle are supported, therefore operating process is relatively complicated and relatively costly.
The content of the invention
The purpose of this utility model is to provide a kind of cell Indirect co-culture support, the existing cell Indirect co-culture of simplification Flow and reduces cost.The technical solution of the utility model is:
A kind of cell Indirect co-culture support, including base and support bar, the base by outer donut and it is interior with one heart Annulus docking is formed, and the thickness of the outer donut is more than the interior donut, and the bottom of two donuts flushes, The joint of the outer donut and the interior donut is provided with groove.
Further, the support bar is the inverted "L" shaped structure for hanging the base.
Cell Indirect co-culture support of the present utility model will coordinate six orifice plates when cell Indirect co-culture is carried out, by one The individual slide for being loaded with cell is placed in six orifice plate bottoms, and another slide for being loaded with cell is placed in into cell Indirect co-culture support On interior donut, then support is put into six orifice plates, you can realize Indirect co-culture process.
The beneficial effects of the utility model are:The utility model simple structure, enormously simplify cell Indirect co-culture mistake Journey, saves experimental period, and make the low raw-material cost that the utility model support is used.Additionally, also repeat making With.
Brief description of the drawings
Fig. 1 is the structural representation of cell Indirect co-culture support of the present utility model;
Fig. 2 is the structural representation of the base that Fig. 1 China and foreign countries donut and interior donut are constituted;
Fig. 3 is the structural representation that the utility model is placed in six orifice plates;
In Fig. 1 ~ 3, the outer donuts of 1-, donut in 2-, 3- grooves, 4- support bars, the orifice plates of 5- six.
Specific embodiment
The utility model is described in further details with specific embodiment below in conjunction with the accompanying drawings, described is to this practicality New explanation is rather than restriction.
Fig. 1 and Fig. 2 provides a kind of structural representation of cell Indirect co-culture support, including base and support bar 4, institute State base to be formed by outer donut 1 and the docking of interior donut 2, the thickness of the outer donut 1 is 0.5mm, a diameter of 31mm, the thickness of interior donut 2 is 0.2mm, and a diameter of 27mm, the bottom of two donuts flushes, the outer concentric circles The joint of ring 1 and the interior donut 2 is provided with the groove 3 for lifting cell culture slide;Support bar is for hanging The inverted "L" shaped structure of the base, quantity is 4.
The cell Indirect co-culture branch is placed in six orifice plates, as shown in figure 3, two kinds of different cells are first distinguished On inoculation slide after treatment, cell attachment is treated;Two slides are respectively placed in six orifice plate bottoms and cell Indirect co-culture On the interior donut of support, according to the characteristics of two kinds of cells, two kinds of intercellular co-cultivations are completed;Taken out from six orifice plates thin Born of the same parents' Indirect co-culture support, lifts and takes out slide using groove.

Claims (2)

1. a kind of cell Indirect co-culture support, it is characterised in that including base and support bar, the base is by outer donut Formed with the docking of interior donut, the thickness of the outer donut is more than the interior donut, and two donuts Bottom flushes, and the joint of the outer donut and the interior donut is provided with groove.
2. a kind of cell Indirect co-culture support according to claim 1, it is characterised in that the support bar is for hanging Hang the inverted "L" shaped structure of the base.
CN201621357418.9U 2016-12-12 2016-12-12 A kind of cell Indirect co-culture support Expired - Fee Related CN206266633U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201621357418.9U CN206266633U (en) 2016-12-12 2016-12-12 A kind of cell Indirect co-culture support

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201621357418.9U CN206266633U (en) 2016-12-12 2016-12-12 A kind of cell Indirect co-culture support

Publications (1)

Publication Number Publication Date
CN206266633U true CN206266633U (en) 2017-06-20

Family

ID=59044031

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201621357418.9U Expired - Fee Related CN206266633U (en) 2016-12-12 2016-12-12 A kind of cell Indirect co-culture support

Country Status (1)

Country Link
CN (1) CN206266633U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107723238A (en) * 2017-09-29 2018-02-23 东北大学 A kind of cell culture apparatus and its application

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107723238A (en) * 2017-09-29 2018-02-23 东北大学 A kind of cell culture apparatus and its application
CN107723238B (en) * 2017-09-29 2020-07-28 东北大学 Cell culture device and application thereof

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GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20170620

Termination date: 20191212