CN209797997U

CN209797997U - easy liquid changing adherent cell culture dish

Info

Publication number: CN209797997U
Application number: CN201822135118.1U
Authority: CN
Inventors: 汪沛; 徐元元; 林彦妮
Original assignee: Suzhou Krui Gene Biotechnology Co Ltd
Current assignee: Suzhou Krui Gene Biotechnology Co Ltd
Priority date: 2018-12-19
Filing date: 2018-12-19
Publication date: 2019-12-17
Anticipated expiration: 2028-12-19

Abstract

The utility model discloses an easily trade liquid adherence cell culture dish, the ware body be bilayer structure, wherein the upper strata is the cell culture layer, the lower floor is the culture medium buffer layer, is equipped with the direct UNICOM culture medium buffer layer of liquid feeding hole on the ware body, still is equipped with the pellicle area of passing through on the cell culture layer. When the culture medium at the lower layer is sucked through the liquid feeding hole, the culture medium at the upper layer can enter the lower layer through the annular groove to be sucked; when the cells are rinsed, the buffer solution can be added into the lower layer through the liquid adding holes, the buffer solution can slowly permeate into the upper layer through the annular groove, and the buffer solution is sucked away through the liquid adding holes after the upper layer is covered; the liquid changing instrument does not directly contact the cell surface in the whole liquid changing process, so that cell pollution is caused, and local high hydraulic pressure cannot be generated to impact cells.

Description

Easy liquid changing adherent cell culture dish

Technical Field

the utility model relates to a culture dish, in particular to a culture dish for adherent cells.

Background

Cell culture is a widely used technique in many biological laboratories, biotechnology or medical companies. Cells can be largely divided into suspension cells, which are mainly suspended in the culture medium, and adherent cells, which are more prone to adhere to the surface of the culture vessel during growth, depending on their growth state. In order to maintain a good nutritional state of cells in the culture process, the culture medium needs to be changed at regular time, supernatant is sucked away for adherent cells, the cells are rinsed by buffer solution, and then the new culture medium is changed. However, for some adherent cells, especially some cells with weak adherence ability, such as HEK293, the following technical problems are easily generated in the process:

1) When the culture medium is sucked, a pipette gun or an electric pipette is generally used for operation, and cells are easily sucked away together with the supernatant;

2) in the cell rinsing process performed by a pipette or an electric pipette, the impact on the cells is caused;

the local pressure may be too high during the addition of the culture medium, which may cause damage to the cells.

Disclosure of Invention

The present invention aims to overcome the above-mentioned shortcomings of the background art, and provides a cell culture dish design for culturing adherent cells, providing a buffer space for cell liquid exchange, and reducing the damage of the operation to the cells.

The technical scheme of the utility model is that: the utility model provides an easily trade liquid adherence cell culture dish, characterized by the dish body be bilayer structure, wherein the upper strata is the cell culture layer, and the lower floor is the culture medium buffer layer, is equipped with the direct UNICOM culture medium buffer layer of liquid feeding hole on the dish body, still is equipped with the semi-permeable membrane region on the cell culture layer.

The utility model has the advantages that: by using the culture dish with the structure, the surface area of cell culture is not affected, but the operation of cell liquid change is simpler. When the culture medium at the lower layer is sucked through the liquid feeding hole, the culture medium at the upper layer can enter the lower layer through the annular groove to be sucked; when the cells are rinsed, the buffer solution can be added into the lower layer through the liquid adding holes, the buffer solution can slowly permeate into the upper layer through the annular groove, and the buffer solution is sucked away through the liquid adding holes after the upper layer is covered; the liquid changing instrument does not directly contact the cell surface in the whole liquid changing process, so that cell pollution is caused, and local high hydraulic pressure cannot be generated to impact cells.

Drawings

FIG. 1 is a top view of the embodiment;

FIG. 2 is a sectional view of the present embodiment;

wherein: 1. dish body, 2, cell culture layer, 3, culture medium buffer layer, 4, liquid feeding hole, 5, semi-permeable membrane region.

Detailed Description

In the embodiment shown in fig. 1 and 2, the upper layer of the dish body (1) is a cell culture layer (2), the lower layer of the dish body is a culture medium buffer layer (3), the dish body is provided with a liquid feeding hole (4) which is directly communicated with the culture medium buffer layer, and the cell culture layer is further provided with a semi-permeable membrane region (5).

The height of the liquid feeding hole is consistent with that of the dish body, so that most of the culture medium is kept in the cell culture layer.

The cell culture layer and the culture medium buffer layer are spaced at a distance of 0.1-0.2 mm, and the upper layer and the lower layer are spaced at a smaller distance, so that the waste of the culture medium can be reduced as much as possible.

The semi-permeable membrane region be the ring channel, and be equipped with the semi-permeable membrane on the ring channel, the tectorial membrane aperture of this semi-permeable membrane is 0.5 ~ 1 micron, guarantees that liquid can pass through this semi-permeable membrane, and the cell can't pass through, for example HEK293 cell diameter is about 10 microns.

the steps of using the utility model for replacing adherent cells are as follows:

1) The process of beginning cell culture is similar general culture dish operation, and after the cell count, take suitable cell number and add upper cell culture layer, because the pellicle is not permeable to the cell, so the cell can be always in the upper strata, in order to prevent that the culture medium from totally permeating the culture medium buffer layer of lower floor, need to add the culture medium through liquid feeding hole fast, wait the culture medium infiltration upper strata and reach suitable height, stop when about 1 ~ 2 millimeters.

2) When the cell is changed, firstly, the culture medium in the lower layer is sucked through the liquid feeding hole, and then under the sucking pressure, the culture medium in the upper layer can permeate into the lower layer to suck all liquid; adding a buffer solution through the liquid adding hole, and stopping when the buffer solution permeates into the upper layer and covers the cells; sucking all buffer solution through the solution adding hole; adding new culture medium through the liquid adding hole, and repeating the operation 1).

3) The cell digestion operation is similar to that of a common culture dish, the digestive enzyme solution is dripped into the cells on the upper layer, the digestive enzyme solution is sucked away after the cells are covered, the cells are incubated at 37 ℃ for 3-5 minutes, a proper amount of culture medium is added through the liquid adding hole, and after the culture medium slightly permeates into the upper layer, the cell suspension on the upper layer is collected.

Claims

1. The utility model provides an easily trade liquid adherence cell culture dish, characterized by easily trade liquid adherence cell culture dish's dish body is bilayer structure, and wherein the upper strata is the cell culture layer, and the lower floor is the culture medium buffer layer, is equipped with the direct UNICOM culture medium buffer layer of liquid feeding hole on the dish body, still is equipped with the pellicle area of passing through on the cell culture layer.

2. The easy-to-change adherent cell culture dish of claim 1, wherein the height of the liquid filling hole is the same as the height of the dish body.

3. The easy-to-change liquid adherent cell culture dish of claim 1, wherein the cell culture layer is spaced from the medium buffer layer by a distance of 0.1 to 0.2 mm.

4. The easy-to-change adherent cell culture dish of claim 1, wherein the semi-permeable membrane region is an annular groove, and a semi-permeable membrane is arranged on the annular groove, and the pore diameter of a membrane covered by the semi-permeable membrane is 0.5 to 1 micron.