CN216585038U - Bacterial biofilm drug resistance detection device - Google Patents
Bacterial biofilm drug resistance detection device Download PDFInfo
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- CN216585038U CN216585038U CN202123332763.0U CN202123332763U CN216585038U CN 216585038 U CN216585038 U CN 216585038U CN 202123332763 U CN202123332763 U CN 202123332763U CN 216585038 U CN216585038 U CN 216585038U
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Abstract
The utility model provides a bacterial biofilm drug resistance detection device, including holding the component, carrier member and shade component, it forms through dismantling the part combination to hold the component, its inside has bacterial biofilm culture room, the purge chamber, the quick room of medicine and resume and cultivate the room, carrier member installs in holding the component, and cultivate the room with bacterial biofilm respectively, the purge chamber, the quick room of medicine and resume and cultivate the room cooperation, its inside space that places detection solution that has, the shade component is installed on holding the component, and cultivate the room with bacterial biofilm respectively, the purge chamber, the quick room of medicine and resume and cultivate the room cooperation, cultivate the room to bacterial biofilm by the shade component, the purge chamber, the quick room of medicine and resume and cultivate the room and carry out the shade protection. The detection device can be disassembled into an independent bacteria biofilm culturing room, a cleaning room, a drug sensitive room and a recovery culturing room, the bacteria in the culturing room can be cultured by independently disassembling the culturing room, and the storage and the efficiency of solution in each room are not remained.
Description
Technical Field
The utility model discloses microbiological experiment equipment especially relates to a bacterial biofilm drug resistance detection device.
Background
Today, morbidity, mortality, and social and economic costs from antibiotic-resistant bacterial infections are increasing, with an estimated 1000 million people worldwide dying from antibiotic resistance each year by 2050, and the cumulative economic cost will amount to $ 100 trillion. The serious resistance problem presents a huge challenge to the global public health system, where the biofilm resistance hazard is even more non-trivial.
Bacterial biofilms refer to a collection of microorganisms formed by bacteria and their extracellular secretions that adhere to biological or non-biological surfaces, a mode of bacterial survival that corresponds to planktonic bacteria. Biofilm formation poses a significant threat to human health, and statistically, biofilms mediate about 80% of human infections worldwide, and about 65% of chronic infections are directly associated with biofilm formation. The complex physiological structure of the biological membrane endows bacteria with the capability of resisting external adverse conditions, the drug resistance of the bacteria in the biological membrane to antibiotics is obviously higher than that of planktonic bacteria, and once the bacteria are formed, the bacteria are difficult to thoroughly clear, so that the treatment difficulty of infection is greatly increased. Therefore, it is necessary to determine the effective drug concentration to completely inhibit and eliminate biofilm bacteria.
Due to the particularity of biofilms, the existing research is relatively complicated to culture bacterial biofilms and detect drug resistance. In the CLSI standard, the broth microdilution method only aims at antibiotic sensitivity experiments carried out on bacteria in a planktonic state, omits bacteria in a biofilm and does not take the situation that the bacteria form the biofilm into consideration, and the concentration of the antibiotic medicine cannot completely kill the bacteria attached to the surface of protein or biological materials. Therefore, when medication guidance is given to infections caused by bacteria, it is necessary to consider the influence of biofilm formation on bacterial resistance. Therefore, a bacterial biofilm drug resistance detection device is provided, which measures the minimum inhibitory concentration (BIC) and minimum biofilm removal concentration (MBEC) of antibiotics to bacteria in a biofilm and provides a basis for clinical rational medication.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a bacterial biofilm drug resistance detection device provides the basis for clinical reasonable using medicine.
The utility model discloses a solve the technical scheme that its technical problem adopted and be:
a bacterial biofilm resistance detection device comprising:
a containing member formed by combining detachable components, wherein the containing member is internally provided with a bacterial biofilm culturing chamber, a cleaning chamber, a drug sensitive chamber and a recovery culturing chamber;
a bearing member which is arranged in the accommodating member, is respectively matched with the bacterial biofilm culturing chamber, the cleaning chamber, the drug sensitive chamber and the recovery culturing chamber, and is internally provided with a space for placing a detection solution;
and a mask member mounted on the accommodating member and respectively fitted with the bacterial biofilm culturing chamber, the cleaning chamber, the drug sensitive chamber and the recovery culturing chamber, wherein the mask member masks and protects the bacterial biofilm culturing chamber, the cleaning chamber, the drug sensitive chamber and the recovery culturing chamber.
Specifically, the accommodating member includes:
the bacterial biofilm box body, the cleaning box body, the drug sensitive box body and the recovery culture box body are respectively rectangular, the upper ends of the bacterial biofilm box body, the cleaning box body, the drug sensitive box body and the recovery culture box body are open and are mutually combined through a detachable connection structure, and the bacterial biofilm culture chamber, the cleaning chamber, the drug sensitive chamber and the recovery culture chamber are respectively positioned in the bacterial biofilm box body, the cleaning box body, the drug sensitive box body and the recovery culture box body.
The bearing member includes:
the bacterial biofilm culturing cup body, the cleaning cup body, the drug sensitive cup body and the recovery culturing cup body are respectively arranged in a bacterial biofilm culturing room, a cleaning room, a drug sensitive room and a recovery culturing room.
The shade member includes:
the bacterial biofilm culturing cover cap, the cleaning cover cap, the drug sensitive cover cap and the recovery culturing cover cap are respectively arranged at the tops of the bacterial biofilm culturing chamber, the cleaning chamber, the drug sensitive chamber and the recovery culturing chamber and cover the bacterial biofilm culturing cup body, the cleaning cup body, the drug sensitive cup body and the recovery culturing cup body.
The sizes of the bacterial biofilm box body, the cleaning box body, the drug sensitive box body and the recovery culture box body are consistent, and the bacterial biofilm box body, the cleaning box body, the drug sensitive box body and the recovery culture box body are made of polypropylene.
The bacteria biofilm culturing cup body, the cleaning cup body, the drug sensitive cup body and the recovery culturing cup body are respectively in a hollow grid shape.
The bacterial biofilm culturing cover cap, the cleaning cover cap, the drug sensitive cover cap and the recovery culturing cover cap are respectively made of polystyrene.
The top of the bacterial biofilm culturing chamber, the top of the cleaning chamber, the top of the drug sensitive chamber and the top of the recovery culturing chamber are respectively provided with a positioning groove, and the bacterial biofilm culturing cover cap, the bacterial biofilm cleaning cover cap, the bacterial biofilm sensitive cover cap and the bacterial biofilm recovery culturing cover cap are respectively provided with a positioning tenon matched with the bacterial biofilm culturing cover cap.
The bottom of the bacterial biofilm culture cover is provided with a group of inoculating needles which are made of polypropylene and correspond to the bacterial biofilm culture cup bodies one by one.
The utility model has the advantages that:
the detection device has simple structure and reasonable design, and can put the thallus to be detected into the culture chamber and cover the cover plate, and the biomembrane grows and adheres to the inoculation needle; after the thalli are cultured to be mature, the inoculating needle can be moved into a cleaning chamber, and planktonic bacteria can be washed away; then, only inoculating needles of tunica capsulata are connected into a drug sensitive chamber containing antibiotics with different concentrations, and the minimum inhibitory concentration BIC of drug sensitive assay is carried out; finally, the treated biofilm is transferred into a recovery culture chamber for recovery culture to determine the minimum biofilm removal concentration (MBEC).
The detection device can be disassembled into an independent bacteria organism culture room, a cleaning room, a drug sensitive room and a recovery culture room, the culture room can be disassembled independently to culture bacteria in the culture room, and the storage and the efficiency of solution in all the rest rooms can not be influenced. The device is divided into functional areas, so that the determination and evaluation of the drug resistance of the bacterial biofilm are facilitated. The inoculating needle is made of polystyrene material, which is beneficial to the attachment and growth of bacterial biofilm. The method is convenient to operate, can directly obtain the bacterial biofilm and perform drug sensitivity detection, greatly reduces the difficulty of drug resistance detection of the bacterial biofilm, and provides a basis for clinical reasonable medication.
Drawings
Fig. 1 is a schematic structural diagram of a bacterial biofilm drug resistance detection device provided by the present invention;
fig. 2 is a schematic side view of the detecting device.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings in the embodiments of the present invention are combined below to clearly and completely describe the technical solutions in the embodiments of the present invention. It is to be understood that the embodiments described are only some of the embodiments of the present invention, and not all of them. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
As figure 1, as shown in figure 2, the utility model provides a bacteria biofilm drug resistance detection device is including holding the component, carrier member and shade component, it forms through dismantling the part combination to hold the component, it has bacteria biofilm culture room to its inside, the purge chamber, the quick room of medicine and resume the culture room, carrier member installs in holding the component, and respectively with bacteria biofilm culture room, the purge chamber, the quick room of medicine and resume the culture room cooperation, it has the space of placing detection solution to its inside, the shade component is installed on holding the component, and respectively with bacteria biofilm culture room, the purge chamber, the quick room of medicine and resume the culture room cooperation, by shade component to bacteria biofilm culture room, the purge chamber, the quick room of medicine and resume the culture room and carry out the shade protection. In this embodiment, the accommodating member includes a bacterial biofilm box 110, a cleaning box 120, a drug sensitive box 130 and a recovery box 140, the bacterial biofilm box, the cleaning box, the drug sensitive box and the recovery box are rectangular respectively, the upper ends thereof are open, and are combined with each other through a detachable connection structure, and the bacterial biofilm culturing chamber, the cleaning chamber, the drug sensitive chamber and the recovery box are respectively located in the bacterial biofilm box, the cleaning box, the drug sensitive box and the recovery box. The bearing component comprises a bacterial biofilm culturing cup body 210, a cleaning cup body 220, a drug sensitive cup body 230 and a recovery culturing cup body 240, wherein the bacterial biofilm culturing cup body, the cleaning cup body, the drug sensitive cup body and the recovery culturing cup body are respectively arranged in a bacterial biofilm culturing room, a cleaning room, a drug sensitive room and a recovery culturing room. The mask component comprises a bacterial biofilm culturing cover cap 310, a cleaning cover cap 320, a drug sensitive cover cap 330 and a recovery culturing cover cap 340, wherein the bacterial biofilm culturing cover cap, the cleaning cover cap, the drug sensitive cover cap and the recovery culturing cover cap are respectively arranged at the tops of a bacterial biofilm culturing chamber, a cleaning chamber, a drug sensitive chamber and a recovery culturing chamber and cover the bacterial biofilm culturing cup body, the cleaning cup body, the drug sensitive cup body and the recovery culturing cup body. The sizes of the bacterial biofilm box body, the cleaning box body, the drug sensitive box body and the recovery culture box body are consistent, the bacterial biofilm box body, the cleaning box body, the drug sensitive box body and the recovery culture box body are made of polypropylene, and the inner walls of the bacterial biofilm box body, the cleaning box body, the drug sensitive box body and the recovery culture box body are respectively provided with a sealing ring matched with the bacterial biofilm culture cup body, the cleaning cup body, the drug sensitive cup body and the recovery culture cup body for preventing liquid leakage. The bacteria biofilm culturing cup body, the cleaning cup body, the drug sensitive cup body and the recovery culturing cup body are respectively in a hollow grid shape. The bacterial biofilm culturing cover cap, the cleaning cover cap, the drug sensitive cover cap and the recovery culturing cover cap are respectively made of polystyrene. The top of the bacterial biofilm culturing chamber, the top of the cleaning chamber, the top of the drug sensitive chamber and the top of the recovery culturing chamber are respectively provided with a positioning groove, and the bacterial biofilm culturing cover cap, the bacterial biofilm cleaning cover cap, the bacterial biofilm sensitive cover cap and the bacterial biofilm recovery culturing cover cap are respectively provided with a positioning tenon matched with the bacterial biofilm culturing cover cap. The bottom of the bacterial biofilm culture cover cap is provided with a group of inoculating needles 311 which are made of polypropylene and correspond to the bacterial biofilm culture cup bodies one by one, and the bacterial biofilm culture cover cap provided with the inoculating needles can replace a cleaning cover cap, a drug sensitive cover cap and a recovery culture cover cap at will for relevant operations.
The using method of the detection device comprises the following steps:
1. and (3) culturing bacterial biofilm: adding a certain amount of required measured thallus into a micropore cup of a biofilm culture chamber containing a culture solution, covering an inoculating needle, culturing in an environment required by the growth of a bacterial strain biofilm until the bacterial biofilm is mature, and attaching the bacterial biofilm to the inoculating needle.
2. Cleaning the biofilm: taking off the upper cover of the inoculating needle adhered with the mature bacteria biofilm, putting the inoculating needle into a micropore cup of a cleaning chamber containing cleaning fluid with certain concentration, and cleaning for several times to wash off free bacteria on the inoculating needle.
3. And (3) drug sensitivity determination: covering the inoculating needle with the floating bacteria washed off in drug sensitive cups containing antibiotics with different concentrations, performing antibiotic treatment, and measuring the minimum inhibitory concentration (BIC) value after culturing.
4. And (3) recovery culture: the inoculating needle treated by the antibiotics is washed in a cleaning room to remove adhered dead bacteria and residual antibiotics, and is put into a microporous cup of a recovery culture room containing fresh culture solution for recovery culture, and the minimum biofilm removal concentration (MBEC) is determined.
The utility model discloses be suitable for any biofilm fungus, culture solution, washing liquid and antibiotic kind in each function room can add according to researcher's actual demand, and used material is suitable for the growth of bacterium biofilm. The utility model discloses simple structure, convenient operation has fine flexibility and practicality.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "left", "right", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, or orientations or positional relationships that are conventionally placed when the products of the present invention are used, or orientations or positional relationships that are conventionally understood by those skilled in the art, and are only for convenience of description of the present invention and simplification of description, but do not indicate or imply that the designated devices or elements must have a specific orientation, be configured and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance. In the description of the present invention, it should be further noted that, unless explicitly stated or limited otherwise, the terms "disposed" and "connected" are to be interpreted broadly, and for example, "connected" may be a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; the connection may be direct or indirect via an intermediate medium, and may be a communication between the two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
Claims (9)
1. A bacterial biofilm resistance test device, comprising:
a containing member formed by combining detachable components, wherein the containing member is internally provided with a bacterial biofilm culturing chamber, a cleaning chamber, a drug sensitive chamber and a recovery culturing chamber;
a bearing component which is arranged in the accommodating component, is respectively matched with the bacterial biofilm culturing chamber, the cleaning chamber, the drug sensitive chamber and the recovery culturing chamber, and is internally provided with a space for placing a detection solution;
and a mask member mounted on the accommodating member and respectively fitted with the bacterial biofilm culturing chamber, the cleaning chamber, the drug sensitive chamber and the recovery culturing chamber, wherein the mask member masks and protects the bacterial biofilm culturing chamber, the cleaning chamber, the drug sensitive chamber and the recovery culturing chamber.
2. A bacterial biofilm resistant test apparatus as claimed in claim 1, wherein said receiving means comprises:
the bacterial biofilm box body, the cleaning box body, the drug sensitive box body and the recovery culture box body are respectively rectangular, the upper ends of the bacterial biofilm box body, the cleaning box body, the drug sensitive box body and the recovery culture box body are open and are mutually combined through a detachable connection structure, and the bacterial biofilm culture chamber, the cleaning chamber, the drug sensitive chamber and the recovery culture chamber are respectively positioned in the bacterial biofilm box body, the cleaning box body, the drug sensitive box body and the recovery culture box body.
3. The apparatus of claim 2, wherein the carrier member comprises:
the bacterial biofilm culturing cup body, the cleaning cup body, the drug sensitive cup body and the recovery culturing cup body are respectively arranged in a bacterial biofilm culturing room, a cleaning room, a drug sensitive room and a recovery culturing room.
4. A bacterial biofilm resistant detection apparatus as claimed in claim 3, wherein said mask means comprises:
the bacterial biofilm culturing cover cap, the cleaning cover cap, the drug sensitive cover cap and the recovery culturing cover cap are respectively arranged at the tops of the bacterial biofilm culturing chamber, the cleaning chamber, the drug sensitive chamber and the recovery culturing chamber and cover the bacterial biofilm culturing cup body, the cleaning cup body, the drug sensitive cup body and the recovery culturing cup body.
5. The apparatus of claim 2, wherein:
the sizes of the bacterial biofilm box body, the cleaning box body, the drug sensitive box body and the recovery culture box body are consistent, and the bacterial biofilm box body, the cleaning box body, the drug sensitive box body and the recovery culture box body are made of polypropylene.
6. The apparatus of claim 3, wherein:
the bacteria biofilm culturing cup body, the cleaning cup body, the drug sensitive cup body and the recovery culturing cup body are respectively in a hollow grid shape.
7. The apparatus of claim 3, wherein:
the bacterial biofilm culturing cover cap, the cleaning cover cap, the drug sensitive cover cap and the recovery culturing cover cap are respectively made of polystyrene.
8. The apparatus of claim 3, wherein:
the top of the bacterial biofilm culturing chamber, the top of the cleaning chamber, the top of the drug sensitive chamber and the top of the recovery culturing chamber are respectively provided with a positioning groove, and the bacterial biofilm culturing cover cap, the bacterial biofilm cleaning cover cap, the bacterial biofilm sensitive cover cap and the bacterial biofilm recovery culturing cover cap are respectively provided with a positioning tenon matched with the bacterial biofilm culturing cover cap.
9. The apparatus of claim 3, wherein:
the bottom of the bacterial biofilm culture cover is provided with a group of inoculating needles which are made of polypropylene and correspond to the bacterial biofilm culture cup bodies one by one.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123332763.0U CN216585038U (en) | 2021-12-28 | 2021-12-28 | Bacterial biofilm drug resistance detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123332763.0U CN216585038U (en) | 2021-12-28 | 2021-12-28 | Bacterial biofilm drug resistance detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216585038U true CN216585038U (en) | 2022-05-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123332763.0U Active CN216585038U (en) | 2021-12-28 | 2021-12-28 | Bacterial biofilm drug resistance detection device |
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| CN (1) | CN216585038U (en) |
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2021
- 2021-12-28 CN CN202123332763.0U patent/CN216585038U/en active Active
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