CN202482308U - Culture and drug sensitivity integrated detection board containing special gas for special microorganisms - Google Patents
Culture and drug sensitivity integrated detection board containing special gas for special microorganisms Download PDFInfo
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- CN202482308U CN202482308U CN 201220101735 CN201220101735U CN202482308U CN 202482308 U CN202482308 U CN 202482308U CN 201220101735 CN201220101735 CN 201220101735 CN 201220101735 U CN201220101735 U CN 201220101735U CN 202482308 U CN202482308 U CN 202482308U
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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Abstract
The utility model discloses a culture and drug sensitivity integrated detection board containing special gas for special microorganisms. The detection board comprises a board body, a self-sealing rubber plug and a board cover, and is structurally characterized in that two rows of detection holes are formed on the board body; a puncture hole is formed in the middle of the self-sealing rubber plug; the bottom of the puncture hole is made into a thin layer; the board cover is provided with an opening corresponding to the detection hole on the board body in position; the diameter of the opening is less than that of the self-sealing rubber plug; and after the self-sealing rubber plug is plugged in the detection hole of the board body, the board body is covered with the board cover. The detection board disclosed by the utility model has low requirements on basic conditions, the culture of the special pathogenic microorganisms and drug sensitivity tests can be performed at the same time simply by virtue of a common constant-temperature incubator instead of an expensive special incubator, and the culture and the test are not limited by the types of the pathogenic microorganisms; and the different types of pathogenic microorganisms with different requirements can be cultured in the one common constant-temperature incubator at the same time. An operator of a clinical laboratory only needs to inject a collected patient specimen into a tube through a pipettor and put the pipettor in the common constant-temperature incubator.
Description
Technical field
The utility model belongs to medicine and hygiene fields, especially belongs to a kind of peculiar microorganism and uses cultivation, the susceptibility one check-out console that contains special gas.
Background technology
Microorganism culturing is used on clinical detection very extensively, and Chang Zuowei " gold standard " is applied to medical diagnosis on disease.
Different pathogenic micro-organisms has demands of different to the growth ring condition; Like nutritive substance, growth temperature, air conditions ... Deng; The human required nutritive substance of most of pathogenic micro-organism process of growth of findding out basically; Also there is the required substratum sale of finished goods of different microorganisms growth in market, and is easy to use.Then be very easy to control for growth temperature.But it is very harsh that some pathogenic micro-organism requires the required gas condition of growing, and promptly can not grow or poor growth if reach requirement, brings difficulty even mistaken diagnosis to clinical diagnosis.
Air conditions there is being the mikrobe of requirement roughly be divided into: anerobes, aerophil, microaerobe; Even except that requiring trace oxygen, also need other mixed gass in some microaerobe process of growth; Increased difficulty for clinical cultivation; Have 80-90% hospital can't carry out this type pathogenic micro-organism approximately cultivates in the whole nation; Because the cultivation of this type pathogenic micro-organism needs expensive extraordinary incubator, and an incubator once can only cultivate a kind of pathogenic micro-organism, and operator are essential will be through professional training.
At present, hospital generally can only carry out the cultivation of anerobes and common aerophil, but in operating process, still has the factor that is unfavorable for the pathogenic micro-organism growth.Such as: candle jar method is adopted in the cultivation for anerobes always, be about to substratum and put into a Glass Containers, short candle of internal combustion and with the container cover sealing, candle extinguishes during oxygen in candle exhausts container, container interior anaerobic state.But the materials such as smog that discharge in the candle combustion process have directly influenced microbial growth also, are to cause pathogenic bacteria in one of late stage of culture poor growth or odd-shaped reason.Often do not have particular requirement for common aerophil, but the pathogenic micro-organism that needs high oxygen concentration to cultivate is not then reached culture condition at common incubator, cause the pathogenic bacteria poor growth.
In today of abuse of antibiotics, most of mikrobe has produced resistance (resistance) to some microbiotic, and in the diagnosis and treatment process of the routine that means such as no drug sensitive experiment instruct, which medicaments insensitive the doctor can't judge mikrobe to, to which medicine resistance.Therefore in therapeutic process, possibly institute's medicament be the medicine of resistance,, cause the disease obstinate failing to respond to any medical treatment, but the reason of can not find.Therefore drug sensitive experiment has in the antimicrobial therapy and has very important significance.Regrettably there are a lot of medical institutions can't carry out special row microorganism culturing and drug sensitive experiment at present because of the condition reason.
Summary of the invention
The purpose of the utility model is to provide a kind of medical institutions to need not that particular facility can be cultivated extraordinary pathogenic agent simultaneously and the peculiar microorganism of drug sensitive experiment uses cultivation, the susceptibility one check-out console that contains special gas; Substratum that specific disease pathogenic microorganism growth is required and special gas charge in advance and detect in the hole, and the operator of clinical laboratory only need patient's sample of being gathered through in the pipettor filling orifice and put into common incubator and get final product.Hospital does not need expensive extraordinary incubator; More do not need operator to carry out loaded down with trivial details operation; Only need in common constant incubator, just to carry out the specific disease pathogenic microorganism and cultivate drug sensitive experiment; And not limited by the pathogenic micro-organism kind, the logical constant incubator of a Daepori can be cultivated the pathogenic micro-organism of dissimilar demands of different simultaneously, makes peculiar microorganism cultivation and drug sensitive experiment become more convenient, quicker, more economical, quicker.The most important thing is provides the most effectively medication foundation for clinical treatment.
The purpose of the utility model is achieved in that described peculiar microorganism uses cultivation, the susceptibility one check-out console that contains special gas, comprises plate body, self-enclosed plug and Ban Gai, and its constructional feature is provided with two gaging holes of listing and indexing for plate body; Self-enclosed plug middle part is a puncturing hole, processes thin layer at the bottom of the hole of puncturing hole; Plate cover be provided with plate body on the corresponding opening in detection hole site, opening diameter is less than the diameter of self-enclosed plug, the detection stopple of plate body is stamped the plate lid after self-enclosed plug is arranged outside plate body.
The plate body periphery is provided with a wire projection buckle bar; Enclose in the plate lid put one with the corresponding buckle bar of wire projection buckle bar of plate body, the wire projection buckle bar that encloses in the plate lid is placed corresponding buckle bar fastening outward with plate body.
Corresponding negative control hole, positive detection hole, lower concentration susceptibility detection hole and the high density susceptibility of being distributed with detects the hole in the described detection hole.
The advantage of the utility model: the utility model compared with prior art reaches more useful effect and is:
1, basic condition requires low: do not need expensive extraordinary incubator; Only need in common constant incubator, just can carry out simultaneously the cultivation and the drug sensitive experiment of specific disease pathogenic microorganism; And not limited by the pathogenic micro-organism kind, the logical constant incubator of a Daepori can be cultivated the pathogenic micro-organism of dissimilar demands of different simultaneously.
2, simple to operate: the operator of clinical laboratory only need patient's sample of being gathered through in the pipettor injection tube and put into common constant incubator and get final product.
3, applied widely: any hospital all can carry out the cultivation and the susceptibility of specific disease pathogenic microorganism, need not particular facility.
But 4 expanded ranges are wide: this cultivation susceptibility is analysed and can be charged into gas with various by the different microorganisms requirement of growing, and can be used for all kinds of microorganism culturing and drug sensitive experiment.
5, economy: the product cost alleviates patient economy burden.
Description of drawings
Fig. 1 is the plate lid structural representation of the utility model.
Fig. 2 is the plate body structure synoptic diagram of the utility model.
Fig. 3 is the schematic perspective view of the self-enclosed plug of the utility model.
Fig. 4 is the profile structural representation of Fig. 1.
Fig. 5 has the profile structural representation of self-enclosed plug for the detection stopple of the plate body of the utility model.
Fig. 6 is stamped the profile structural representation that plate covers after the detection stopple of the plate body of the utility model has self-enclosed plug outside plate body.
Among the figure: plate body 1, self-enclosed plug 2, plate lid 3 detects hole 4, wire projection buckle bar 5, buckle bar 6, opening 7, puncturing hole 8.
Embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is elaborated:
Like Fig. 1, Fig. 2, Fig. 3, Fig. 4, Fig. 5 and shown in Figure 6; The described peculiar microorganism of the utility model uses cultivation, the susceptibility one check-out console that contains special gas; Comprise plate body 1, self-enclosed plug 2 and plate lid 3; Said plate body 1 is processed like the transparent PVC material by transparent material, is convenient to observe the microorganism growth situation; Its constructional feature is that plate body 1 is provided with two gaging holes 4 of listing and indexing, and negative control hole, positive detection hole, lower concentration susceptibility is detected hole and high density susceptibility detect the hole and carry out subregion; Plate body 1 periphery is provided with a wire projection buckle bar 5, makes to enclose the corresponding buckle bar 6 that is provided with in itself and the plate lid 3 and closely engage.Self-enclosed plug 2 is processed by soft rubber material or silica gel, and material should have enough rebound resiliences, makes behind the puncturing hole puncture application of sample tight closure rapidly; Self-enclosed plug 2 middle parts are puncturing hole 8, process thin layer at the bottom of the hole of puncturing hole, are convenient to puncture and add sample or culture.Plate lid 3 is processed by materials such as transparent PVCs, so that observe the microorganism culturing situation; Plate lid 3 be provided with plate body on the corresponding opening 7 in detection hole site, opening 7 diameters are less than the diameter of self-enclosed plug 2; Enclose in the plate lid 3 put one with the wire projection buckle bar 5 corresponding buckle bars 6 of plate body, make the closely engaging of plate body plate lid.During detection, the required special culture media of extraordinary pathogenic micro-organism growth is packed in the detection hole of plate body, in detecting the hole, charge into desired gas, the good self-enclosed plug of plug is buckled well the plate lid.The envelope film is preserved.During use, the puncturing hole of pathogenic micro-organism suspension through self-enclosed plug injected in the detection hole, subsequently cultivation, the susceptibility one check-out console of the utility model are put into constant incubator and got final product with pipettor.
Claims (3)
1. a peculiar microorganism uses cultivation, the susceptibility one check-out console that contains special gas, comprises plate body (1), self-enclosed plug (2) He Bangai (3), it is characterized in that plate body (1) is provided with two gaging holes (4) of listing and indexing; Self-enclosed plug (2) middle part is puncturing hole (8), processes thin layer at the bottom of the hole of puncturing hole; Plate lid (3) be provided with plate body on the corresponding opening in detection hole site (7); Opening (7) diameter is less than the diameter of self-enclosed plug (2), and the detection hole (4) of plate body (1) is plugged with self-enclosed plug (2) back and outside plate body, is stamped plate lid (3).
2. peculiar microorganism according to claim 1 uses cultivation, the susceptibility one check-out console that contains special gas, it is characterized in that plate body (1) periphery is provided with a wire projection buckle bar (5); Enclose in the plate lid (3) put one with the corresponding buckle bar of wire projection buckle bar (5) (6) of plate body, the wire projection buckle bar (6) that encloses in the plate lid (3) is placed corresponding buckle bar (5) outward with plate body and is fastened.
3. peculiar microorganism according to claim 1 and 2 uses cultivation, the susceptibility one check-out console that contains special gas, it is characterized in that corresponding negative control hole, positive detection hole, lower concentration susceptibility detection hole and the high density susceptibility of being distributed with detects the hole in the described detection hole (4).
Priority Applications (1)
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CN 201220101735 CN202482308U (en) | 2012-03-19 | 2012-03-19 | Culture and drug sensitivity integrated detection board containing special gas for special microorganisms |
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CN 201220101735 CN202482308U (en) | 2012-03-19 | 2012-03-19 | Culture and drug sensitivity integrated detection board containing special gas for special microorganisms |
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CN202482308U true CN202482308U (en) | 2012-10-10 |
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CN 201220101735 Expired - Fee Related CN202482308U (en) | 2012-03-19 | 2012-03-19 | Culture and drug sensitivity integrated detection board containing special gas for special microorganisms |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2016166315A1 (en) * | 2015-04-16 | 2016-10-20 | Insphero Ag | System for propagating cells |
ITUB20151992A1 (en) * | 2015-07-08 | 2017-01-08 | Ivtech S R L | MULTI-COCKPIT PLATE FOR CELL CULTURE |
-
2012
- 2012-03-19 CN CN 201220101735 patent/CN202482308U/en not_active Expired - Fee Related
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2016166315A1 (en) * | 2015-04-16 | 2016-10-20 | Insphero Ag | System for propagating cells |
US10982180B2 (en) | 2015-04-16 | 2021-04-20 | Insphero Ag | System for propagating cells |
ITUB20151992A1 (en) * | 2015-07-08 | 2017-01-08 | Ivtech S R L | MULTI-COCKPIT PLATE FOR CELL CULTURE |
EP3115449A1 (en) * | 2015-07-08 | 2017-01-11 | IVTech S.r.l. | Multi-well plate for cell cultures |
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Legal Events
Date | Code | Title | Description |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C17 | Cessation of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121010 Termination date: 20140319 |