CN201678683U

CN201678683U - Candida fast-identification culture drug susceptibility kit

Info

Publication number: CN201678683U
Application number: CN2009202452629U
Authority: CN
Inventors: 钱叶林; 王敏
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-11-16
Filing date: 2009-11-16
Publication date: 2010-12-22
Anticipated expiration: 2019-11-16

Abstract

The utility model discloses a candida fast-identification culture drug susceptibility kit, comprising a kit and a drug susceptibility board, wherein the kit comprises a kit body, and the inner part of the kit body is provided with an ultra-pure water reagent, a paraffin oil reagent, a physiological saline reagent and a candida fast-identification culture medium; and the drug susceptibility board comprises a board body and a board cover. The board body is provided with a negative testing hole, a positive testing hole and a plurality of medicinal contrast holes corresponding to the negative testing hole and the positive testing hole, and the medicinal contrast holes corresponding to the positive testing hole are filled with testing drugs. The candida fast-identification culture drug susceptibility kit has the advantages of simple operation and short testing cycle.

Description

A kind of candidiasis Rapid identification is cultivated the susceptibility test kit

Technical field

The utility model belongs to the vitro diagnostic techniques field, is specifically related to a kind of candidiasis Rapid identification and cultivates the susceptibility test kit.

Background technology

Candidiasis is a kind of of fungi, cause that the morbific candidiasis of people mainly contains Candida albicans, Oidium tropicale, Candida glabrata, candida krusei and other candidiasis, candidiasis extensively is present in healthy people's skin, oral cavity, respiratory tract and vagina etc. to be located, and belongs to conditioned pathogen.Candidiasis property can cause a lot of diseases, as mucous membrane dermatocandidiasis, white mouth or oropharynx moniliosis, deep organ moniliosis, candidemia, eye moniliosis, kidney moniliosis, liver spleen moniliosis, heart moniliosis, central nervous system moniliosis, muscle skeleton moniliosis and colpitis mycotica etc., colpitis mycotica is a kind of common gynecological disease, mainly be by the microbial colpitis of beads, it is a principal character with pruritus vulvae, cusalgia, bean dregs sample leukorrhea.Candidiasis leads to complications and also has variedly, and respiratory system can concurrent pneumonia, and digestive tube can concurrent enterorrhagia, the intestinal perforation urinary system can concurrent renal failure etc.

What be used for oidiomycetic evaluation cultivation in the market mainly is culture method, and the quick medicine-sensitive test products does not then almost have.Common diagnostic method is, cultivate about 2～3 days by flat board after, observe colonial morphology, be defined as after the candidiasis, picking colony is analyzed resistance with paper disk method again, diagnosis is finished in front and back approximately needs 4～10 days time.Operator are had higher requirements, and waste time and energy, be not suitable for the requirement of clinical quick diagnosis.

The utility model content

The purpose of this utility model provides a kind of candidiasis Rapid identification and cultivates the susceptibility test kit, has solved the problem that wastes time and energy that existing diagnostic method exists.

The technical scheme that the utility model adopted is, a kind of candidiasis Rapid identification is cultivated the susceptibility test kit, comprise test kit and drug sensitive plate, test kit comprises box body, be placed with ultrapure water reagent, paraffin oil reagent, physiological saline reagent and candidiasis Rapid identification substratum in the box body, drug sensitive plate comprises plate body and Ban Gai, plate body is provided with negative hole and the positive detection hole detected, detect in the property of medicine contrast hole that hole and positive detection hole be provided with several property of medicine contrast holes and positive detection hole correspondence corresponding to feminine gender detection of drugs is housed.

The beneficial effects of the utility model are,

(1) improves the candidiasis substratum, add quick growth factor therein, make oidiomycetic cultivation break through about 7 days time limitation of culture-based method, can finish oidiomycetic cultivation in 6～24 hours, its composition mainly comprises peptone, glucose, yeast extract, indicator, quick growth factor etc.In addition, substratum is processed as dry powder, helps the prolonged preservation of product, with using after the supporting ultrapure water dissolving.Simultaneously supporting have a ultrapure water, both uses for convenience, and what also cause for fear of the difference of different areas water quality can not expected result, has guaranteed product stability of the present utility model on to greatest extent.Also be equipped with stroke-physiological saline solution, be used for moistening cotton swab when gathering sample, in the sample short period of time that can guarantee to collect can be dead because of the change of environment, guarantee the accuracy of sample cultivation results.

(2) the method principle that medicine sensitive detecting plate adopted is to improve to form on the basis of the trace liquid diluting method principle of the NCCLS of international standard.Conventional trace liquid diluting method is that the bacterium colony through the screening of mirror mirror is carried out enlarged culturing, more cultured nutrient solution is joined in the good antibiotic concentration gradient of prior dilution, cultivates observations.Because this method operation steps is more, the drug sensitive test of finishing a case approximately needs about 7～8 days time, and culture success ratio is lower, and needs the dedicated experiments personnel to operate.The utility model drug sensitive plate comprises susceptibility plate body and Ban Gai, first row are provided with control wells in the left side of drug sensitive plate, comprise positive and negative control reacting hole, the every row reacting hole that begins from secondary series all corresponding to a kind of antibiotic medicine commonly used at present, comprising: itraconazole, nystatin, amphotericin, flucytosine, miconazole, KETOKONAZOL, econazole, clotrimazole, fluconazole, Lamisil, Mepartricin, Ciclopirox Olamine, Caspofungin, trichomycin.And every kind of microbiotic all has corresponding two concentration-response holes of height on drug sensitive plate, and all microbiotic all adopt the heat bag to be wrapped in advance to be advanced in the drug sensitive plate reacting hole by method.Each susceptibility plate body all has a drug sensitive plate lid corresponding, can prevent that extraneous dust or other foreign material from falling into the susceptibility hole or scratching the drug sensitive reaction hole, influences the accuracy of drug sensitive test.Be equipped with the paraffin oil after the sterilization simultaneously, be used in the drug sensitive test process, sealing drug sensitive reaction hole prevents the volatilization of liquid to greatest extent, influences result's judgement.

(3) the utility model can be in 24 hours a step finish candidiasis and cultivate and medicament sensitivity test, shorten candidiasis incubation time and medicament sensitivity test greatly, be suitable for the monitoring of clinical early diagnosis and clinical candidiasis resistance, provide reasonable use regimen of antibiotics for clinical, prevent antibiotic abuse, shorten patient's course of disease, alleviate patient economy burden.

Description of drawings

Fig. 1 is the structural representation of test kit in the utility model susceptibility test kit;

Fig. 2 is the structural representation of drug sensitive plate in the utility model susceptibility test kit;

Fig. 3 is the structural representation of plate lid in the utility model susceptibility test kit.

Among the figure, 1. box body, 2. ultrapure water reagent, 3. paraffin oil reagent, 4. physiological saline reagent, 5. candidiasis Rapid identification substratum, the 6. negative hole of detecting, 7. positive detection hole, 8. property of medicine contrast hole, 9. plate body, 10. plate lid.

Embodiment

Below in conjunction with the drawings and specific embodiments the utility model is elaborated.

The utility model candidiasis Rapid identification is cultivated the susceptibility test kit and is comprised test kit part and drug sensitive plate part, the structure of test kit as shown in Figure 1, comprise box body 1, be placed with two ultrapure water reagent 2 in the box body 1, a paraffin oil reagent 3, a physiological saline reagent 4 and a candidiasis Rapid identification substratum 5, the structure of drug sensitive plate as shown in Figures 2 and 3, comprise plate body 9 and plate lid 10, plate body 9 is provided with negative hole 6 and the positive detection hole 7 detected, detect in the delegation property of medicine contrast hole 8 that hole 6 and positive detection hole 7 be provided with two row property of medicine contrast holes 8 and positive detection hole 7 correspondences corresponding to feminine gender detection of drugs is housed.

The method of utilizing the utility model susceptibility test kit to detect, specifically implement according to following steps:

Step 1: get a ultrapure water reagent 2 and a candidiasis Rapid identification substratum 5, ultrapure water is joined in the candidiasis Rapid identification substratum 5, after the dissolving, obtain nutrient solution fully;

Step 2: get nutrient solution 50～150 μ l that the step obtains, the feminine gender that joins on the drug sensitive plate detects in the hole 6;

Step 3: get a physiological saline reagent 4, adsorb physiological saline with cotton swab, at the twisted several of diseased region, take out cotton swab, place immediately in the nutrient solution that step 1 obtains, stir cotton swab for several times, extract cotton swab, squeeze out wherein liquid as far as possible, take out cotton swab facing to the bottle wall, shake mixing for several times or with oscillator with sample injector piping and druming, obtain mixed nutrient solution;

Step 4: get mixed nutrient solution 50～150 μ l that the step obtains, join respectively in the positive detection hole 7 and property of medicine contrast hole 8 on the drug sensitive plate;

Step 5: get paraffin oil reagent 3, Dropwise 50 μ l paraffin oil in each hole on drug sensitive plate covers plate lid 10, places drug sensitive plate 35～37 ℃ of incubators to cultivate;

Observe in step 6:6～24 hour and read the result, interpretation standard is as follows:

(1) if feminine gender detects hole 6 nondiscolorations, 7 nondiscolorations of positive detection hole then show no candidiasis growth, and remaining hole does not make a decision;

(2) if negative detection hole 6 variable colors then show pollution, invalidate the test;

(3) if feminine gender detects hole 6 nondiscolorations, 7 variable colors of positive detection hole, showing has candidiasis in the sample, judge remaining each hole result;

A: and negative property of medicine contrast hole 8 nondiscolorations of hole 6 correspondences and property of medicine contrast hole 8 nondiscolorations of positive detection hole 7 correspondences, the medicaments insensitive that expression is corresponding to these row of detecting;

B: and negative property of medicine contrast hole 8 nondiscolorations of hole 6 correspondences and property of medicine contrast hole 8 variable colors of positive detection hole 7 correspondences, the medium sensitivity of medicine that expression is corresponding to these row of detecting;

C: and property of medicine contrast hole 8 variable colors of property of medicine contrast hole 8 variable colors of negative detection hole 6 correspondences and positive detection hole 7 correspondences, expression is insensitive to the medicine of this row correspondence.

The utility model candidiasis Rapid identification is cultivated the susceptibility test kit, simple to operate and sense cycle weak point.

Claims

1. a candidiasis Rapid identification is cultivated the susceptibility test kit, it is characterized in that, comprise test kit and drug sensitive plate, described test kit comprises box body (1), be placed with ultrapure water reagent (2) in the box body (1), paraffin oil reagent (3), physiological saline reagent (4) and candidiasis Rapid identification substratum (5), described drug sensitive plate comprises plate body (9) He Bangai (10), plate body (9) is provided with negative hole (6) and positive detection hole (7) detected, detecting hole (6) and positive detection hole (7) corresponding to feminine gender is provided with several property of medicine contrast holes (8) and positive detection hole (7) corresponding property of medicine and contrasts in the hole (8) detection of drugs is housed.