CN201678682U - Susceptibility test kit capable of rapidly identifying pathogenic fungi through culturing - Google Patents
Susceptibility test kit capable of rapidly identifying pathogenic fungi through culturing Download PDFInfo
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- CN201678682U CN201678682U CN2009202452614U CN200920245261U CN201678682U CN 201678682 U CN201678682 U CN 201678682U CN 2009202452614 U CN2009202452614 U CN 2009202452614U CN 200920245261 U CN200920245261 U CN 200920245261U CN 201678682 U CN201678682 U CN 201678682U
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Abstract
The utility model discloses a susceptibility test kit capable of rapidly identifying pathogenic fungi through culturing, which comprises a kit and a drug susceptibility plate, wherein the kit comprises a kit body, and ultrapure water reagents, paraffin oil reagent, normal saline reagent and a culture medium for rapidly identifying pathogenic fungi are placed in the kit body; and the drug susceptibility plate comprises a plate body and a plate cover, a negative test hole and a positive test hole are formed on the plate body, a plurality of contrast holes for drug property corresponding to the negative and the positive test holes are formed on the plate body, and the contrast holes for drug property corresponding to the positive test hole are provided with test drugs therein. The susceptibility test kit has the advantages of simple operation and short test period.
Description
Technical field
The utility model belongs to the vitro diagnostic techniques field, is specifically related to a kind of pathomycete Rapid identification and cultivates the susceptibility test kit.
Background technology
Pathogenic fungus roughly is divided into superficial part fungal infection and deep infection fungi because the position of its infringement is different with clinical manifestation.Wherein the superficial part fungal infection can be divided into skin infections fungi and subcutis fungal infection again; And the deep infection fungi mainly comprises region pathomycete and opportunistic infection fungi.
What be used in the market that the evaluation of pathomycete cultivates mainly is culture method, and the drug sensitive test product does not then almost have.Common diagnostic method is, cultivate about 2～3 days by flat board after, observe colonial morphology, be defined as after the pathomycete, picking colony is analyzed resistance with paper disk method again, diagnosis is finished in front and back approximately needs 10 days time.Operator are had higher requirements, and waste time and energy, be not suitable for the requirement of clinical quick diagnosis.
The utility model content
The purpose of this utility model provides a kind of pathomycete Rapid identification and cultivates the susceptibility test kit, has solved the problem that wastes time and energy that existing diagnostic method exists.
The technical scheme that the utility model adopted is, a kind of pathomycete Rapid identification is cultivated the susceptibility test kit, comprise test kit and drug sensitive plate, test kit comprises box body, be placed with ultrapure water reagent, paraffin oil reagent, physiological saline reagent and pathomycete Rapid identification substratum in the box body, drug sensitive plate comprises plate body and Ban Gai, plate body is provided with negative hole and the positive detection hole detected, detect in the property of medicine contrast hole that hole and positive detection hole be provided with several property of medicine contrast holes and positive detection hole correspondence corresponding to feminine gender detection of drugs is housed.
The beneficial effects of the utility model are,
(1) improves the pathomycete substratum, add quick growth factor therein, make the cultivation of pathomycete break through about 7 days time limitation of culture-based method, can finish the cultivation of pathomycete in 6～24 hours, its composition mainly comprises peptone, glucose, yeast extract, indicator, quick growth factor etc.In addition, substratum is processed as dry powder, helps the prolonged preservation of product, with using after the supporting ultrapure water dissolving.Simultaneously supporting have a ultrapure water, both uses for convenience, and what also cause for fear of the difference of different areas water quality can not expected result, guarantees on to greatest extent.Product stability of the present utility model.Also be equipped with stroke-physiological saline solution, be used for moistening cotton swab when gathering sample, in the sample short period of time that can guarantee to collect can be dead because of the change of environment, guarantee the accuracy of sample cultivation results.
(2) the method principle that medicine sensitive detecting plate adopted is to improve to form on the basis of the trace liquid diluting method principle of the NCCLS of international standard.Conventional trace liquid diluting method is that the bacterium colony through the screening of mirror mirror is carried out enlarged culturing, more cultured nutrient solution is joined in the good antibiotic concentration gradient of prior dilution, cultivates observations.Because this method operation steps is more, the drug sensitive test of finishing a case approximately needs about 7～8 days time, and culture success ratio is lower, and needs the dedicated experiments personnel to operate.The utility model drug sensitive plate comprises susceptibility plate body and Ban Gai, first row are provided with control wells in the left side of drug sensitive plate, comprise positive and negative control reacting hole, the every row reacting hole that begins from secondary series all corresponding to a kind of antibiotic medicine commonly used at present, comprising: itraconazole, nystatin, amphotericin, flucytosine, miconazole, KETOKONAZOL, econazole, clotrimazole, fluconazole, Lamisil, Mepartricin, Ciclopirox Olamine, Caspofungin, trichomycin.And every kind of microbiotic all has corresponding two concentration-response holes of height on drug sensitive plate, and all microbiotic all adopt the heat bag to be wrapped in advance to be advanced in the drug sensitive plate reacting hole by method.Each susceptibility plate body all has a drug sensitive plate lid corresponding, can prevent that extraneous dust or other foreign material from falling into the susceptibility hole or scratching the drug sensitive reaction hole, influences the accuracy of drug sensitive test.Be equipped with the paraffin oil after the sterilization simultaneously, be used in the drug sensitive test process, sealing drug sensitive reaction hole prevents the volatilization of liquid to greatest extent, influences result's judgement.
(3) the utility model can be in 6～24 hours a step finish pathomycete and cultivate and medicament sensitivity test, shorten pathomycete incubation time and medicament sensitivity test greatly, be suitable for the monitoring of clinical early diagnosis and clinical pathomycete resistance, provide reasonable use regimen of antibiotics for clinical, prevent antibiotic abuse, shorten patient's course of disease, alleviate patient economy burden.
Description of drawings
Fig. 1 is the structural representation of test kit in the utility model susceptibility test kit;
Fig. 2 is the structural representation of drug sensitive plate in the utility model susceptibility test kit;
Fig. 3 is the structural representation of plate lid in the utility model susceptibility test kit.
Among the figure, 1. box body, 2. ultrapure water reagent, 3. paraffin oil reagent, 4. physiological saline reagent, 5. pathomycete Rapid identification substratum, the 6. negative hole of detecting, 7. positive detection hole, 8. property of medicine contrast hole, 9. plate body, 10. plate lid.
Embodiment
Below in conjunction with the drawings and specific embodiments the utility model is elaborated.
The utility model pathomycete Rapid identification is cultivated the susceptibility test kit and is comprised test kit part and drug sensitive plate part, the structure of test kit as shown in Figure 1, comprise box body 1, be placed with two ultrapure water reagent 2 in the box body 1, a paraffin oil reagent 3, a physiological saline reagent 4 and a pathomycete Rapid identification substratum 5, the structure of drug sensitive plate as shown in Figures 2 and 3, comprise plate body 9 and plate lid 10, plate body 9 is provided with negative hole 6 and the positive detection hole 7 detected, detect in the delegation property of medicine contrast hole 8 that hole 6 and positive detection hole 7 be provided with two row property of medicine contrast holes 8 and positive detection hole 7 correspondences corresponding to feminine gender detection of drugs is housed.
The method of utilizing the utility model susceptibility test kit to detect, specifically implement according to following steps:
Step 1: get a ultrapure water reagent 2 and a pathomycete Rapid identification substratum 5, ultrapure water is joined in the pathomycete Rapid identification substratum 5, after the dissolving, obtain nutrient solution fully;
Step 2: get nutrient solution 50～150 μ l that the step obtains, the feminine gender that joins on the drug sensitive plate detects in the hole 6;
Step 3: get a physiological saline reagent 4, adsorb physiological saline with cotton swab, at the twisted several of diseased region, take out cotton swab, place immediately in the nutrient solution that step 1 obtains, stir cotton swab for several times, extract cotton swab, squeeze out wherein liquid as far as possible, take out cotton swab facing to the bottle wall, shake mixing for several times or with oscillator with sample injector piping and druming, obtain mixed nutrient solution;
Step 4: get mixed nutrient solution 50～150 μ l that the step obtains, join respectively in the positive detection hole 7 and property of medicine contrast hole 8 on the drug sensitive plate;
Step 5: get paraffin oil reagent 3, Dropwise 50 μ l paraffin oil in each hole on drug sensitive plate covers plate lid 10, places drug sensitive plate 35～37 ℃ of incubators to cultivate;
Observe in step 6:6～24 hour and read the result, interpretation standard is as follows:
(1) if feminine gender detects hole 6 nondiscolorations, 7 nondiscolorations of positive detection hole then show no pathomycete growth, and remaining hole does not make a decision;
(2) if negative detection hole 6 variable colors then show pollution, invalidate the test;
(3) if feminine gender detects hole 6 nondiscolorations, 7 variable colors of positive detection hole, showing has pathomycete in the sample, judge remaining each hole result;
A: and negative property of medicine contrast hole 8 nondiscolorations of hole 6 correspondences and property of medicine contrast hole 8 nondiscolorations of positive detection hole 7 correspondences, the medicaments insensitive that expression is corresponding to these row of detecting;
B: and negative property of medicine contrast hole 8 nondiscolorations of hole 6 correspondences and property of medicine contrast hole 8 variable colors of positive detection hole 7 correspondences, the medium sensitivity of medicine that expression is corresponding to these row of detecting;
C: and property of medicine contrast hole 8 variable colors of property of medicine contrast hole 8 variable colors of negative detection hole 6 correspondences and positive detection hole 7 correspondences, expression is insensitive to the medicine of this row correspondence.
The utility model pathomycete Rapid identification is cultivated susceptibility test kit and detection method, simple to operate and sense cycle weak point.
Claims (1)
1. a pathomycete Rapid identification is cultivated the susceptibility test kit, it is characterized in that, comprise test kit and drug sensitive plate, described test kit comprises box body (1), be placed with ultrapure water reagent (2) in the box body (1), paraffin oil reagent (3), physiological saline reagent (4) and pathomycete Rapid identification substratum (5), described drug sensitive plate comprises plate body (9) He Bangai (10), plate body (9) is provided with negative hole (6) and positive detection hole (7) detected, detecting hole (6) and positive detection hole (7) corresponding to feminine gender is provided with several property of medicine contrast holes (8) and positive detection hole (7) corresponding property of medicine and contrasts in the hole (8) detection of drugs is housed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202452614U CN201678682U (en) | 2009-11-16 | 2009-11-16 | Susceptibility test kit capable of rapidly identifying pathogenic fungi through culturing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202452614U CN201678682U (en) | 2009-11-16 | 2009-11-16 | Susceptibility test kit capable of rapidly identifying pathogenic fungi through culturing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201678682U true CN201678682U (en) | 2010-12-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009202452614U Expired - Fee Related CN201678682U (en) | 2009-11-16 | 2009-11-16 | Susceptibility test kit capable of rapidly identifying pathogenic fungi through culturing |
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| CN (1) | CN201678682U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106350571A (en) * | 2016-08-30 | 2017-01-25 | 北京市农林科学院 | Method for rapidly screening sensitive antibiotic for treating cow mastitis |
| CN106755279A (en) * | 2017-03-23 | 2017-05-31 | 合肥百盛园生物药业有限公司 | Nutrient solution, kit and detection method for detection bacterium vaginitis |
-
2009
- 2009-11-16 CN CN2009202452614U patent/CN201678682U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106350571A (en) * | 2016-08-30 | 2017-01-25 | 北京市农林科学院 | Method for rapidly screening sensitive antibiotic for treating cow mastitis |
| CN106350571B (en) * | 2016-08-30 | 2019-09-24 | 北京市农林科学院 | A kind of method of the sensitive antibiotics of quick screening treatment mastitis for milk cows |
| CN106755279A (en) * | 2017-03-23 | 2017-05-31 | 合肥百盛园生物药业有限公司 | Nutrient solution, kit and detection method for detection bacterium vaginitis |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| DD01 | Delivery of document by public notice |
Addressee: Qian Yelin Document name: Notification of Passing Examination on Formalities |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101222 Termination date: 20151116 |
|
| EXPY | Termination of patent right or utility model |