CN205826329U - Collection is sampled, reacts, is detected as device integrally - Google Patents
Collection is sampled, reacts, is detected as device integrally Download PDFInfo
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- CN205826329U CN205826329U CN201620650350.7U CN201620650350U CN205826329U CN 205826329 U CN205826329 U CN 205826329U CN 201620650350 U CN201620650350 U CN 201620650350U CN 205826329 U CN205826329 U CN 205826329U
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Abstract
The utility model discloses two kinds of devices that collection is sampled, reacted, is detected as one, the first device includes liquid storage pipe, liquid separatnig valve, stator, sampling wiper sub bar and reaction tube, and liquid separatnig valve is provided with broken needle；Stator connects sampling wiper sub bar；Liquid separatnig valve loads liquid storage pipe inner chamber, and the storage intracavity of liquid storage pipe and liquid separatnig valve formation is positioned at storage intracavity equipped with solution, the needle of liquid separatnig valve；Stator loads in liquid storage pipe, and is provided with gap between liquid separatnig valve, places chemical substance in gap；The opening of liquid storage pipe puts reaction tube.The second device comprises all elements of the first device, contain simultaneously can damaged ampoul tube, the needle of liquid separatnig valve is positioned at outside storage chamber.This utility model simple in construction, places plurality of reagents subregion, is used in mixed way before reaction, can be continuously finished the collection of sample, process, reacts and detecting step.Can be widely applied to the Pathogen test of clinical sample, microorganism culturing, biochemical enzymes activity analysis, immunochemistry detection etc..
Description
Technical field
This utility model relates to chemical devices, particularly relates to the device that collection is sampled, reacted, is detected as one.
Background technology
Medical science relates to two parts, i.e. sample collecting and detection.The most effective collection sampling and detection
The reaction unit being integrated, two above steps are carried out separately.Meanwhile, the credibility of testing result depend on and
Time effectively process sample, thus the collection sampling of integral type, react and be detected as the device of one, just can locate in sampling location
Reason sample, not only saves time and has also been correspondingly improved accuracy in detection.
The Chinese utility model patent of Patent No. ZL200510023792.5 discloses the dress of a kind of sample collecting and testing
Put, this device be contained within can damaged valve, can the needle of damaged valve be positioned in reservoir solution.Meanwhile, can damaged valve valve body with
Sampling swab tubule is connected (front end of sampling swab tubule is inserted and can be located between needle and the valve body of damaged valve), formed one whole
Body.Before needle does not fractures, liquid cannot be introduced into the inner chamber of sampling swab.The structure of this device can only be equipped with one in causing liquid storage pipe
Plant reagent, it is impossible to be applied to the integrated device of plurality of reagents reaction, use and have certain limitation.
Utility model content
The technical problems to be solved in the utility model is the device providing collection to sample, reacting, be detected as one, this device
Can apply to the integrated device of plurality of reagents reaction.This apparatus structure is simple, easy to operate, just can locate in sampling location
Reason sample, not only saves time and has also been correspondingly improved accuracy in detection.
For solving above-mentioned technical problem, this utility model following technical scheme of offer:
In one side of the present utility model, it is provided that a kind of collect sampling, react, be detected as one device, this device includes:
Liquid storage pipe, liquid separatnig valve, stator, sampling wiper sub bar, reaction tube；Liquid separatnig valve is provided with broken needle；Stator connects to be adopted
Sample swab handle；Liquid separatnig valve loads liquid storage pipe inner chamber, and the storage intracavity of liquid storage pipe and liquid separatnig valve formation is equipped with solution, the valve of liquid separatnig valve
Pin is positioned at described storage intracavity；Stator loads in liquid storage pipe, and is provided with gap between liquid separatnig valve, places chemicals in gap
Matter；The opening of liquid storage pipe puts reaction tube.
It is preferably, in described gap, is additionally provided with porous membrane.
Being preferably, one end of described sampling wiper sub bar is provided with aseptic cotton carrier, inoculating loop or spiral sampling cone, and the other end inserts
In the cavity of stator.
Being preferably, described liquid storage pipe is made up of clear supple material；Described reaction tube is for having resilient transparent flexible pipe
Body.
Being preferably, the tail end of described reaction tube is provided with the adapter of band snap bar, and sets trocar sheath and be enclosed within outside adapter；
Described adapter cavity hollow, snap bar is solid.Break the snap bar of the adapter of the tail end of disconnected reaction tube, take out reaction tube
Trocar sheath, the outer wall of extruding reaction pipe, liquid flows out from adapter port, oozes detection after can be used for reactant mixing.
In one side of the present utility model, it is provided that another kind of collection is sampled, reacts, is detected as device integrally, this device bag
Include: liquid storage pipe, can damaged ampoul tube, liquid separatnig valve, stator, sampling wiper sub bar, reaction tube；Liquid separatnig valve is provided with broken valve
Pin；Stator connects sampling wiper sub bar；Liquid separatnig valve loads bottom liquid storage pipe, the storage intracavity that liquid storage pipe and liquid separatnig valve are formed equipped with
Solution, the needle of liquid separatnig valve is positioned at outside described storage chamber；Described can be preinstalled with chemical substance by damaged ampoul tube, and deposit described in loading
In storage chamber；Stator loads in liquid storage pipe, and is provided with gap between liquid separatnig valve, places chemical substance in gap；Opening of liquid storage pipe
Mouth end puts reaction tube.
It is preferably, in described gap, is additionally provided with porous membrane.
Being preferably, described sampling wiper sub bar inserts in stator, and stator loads liquid storage pipe inner chamber.
Being preferably, one end of described sampling wiper sub bar is provided with aseptic cotton carrier, inoculating loop or spiral sampling cone, and the other end inserts
In the cavity of stator.
Being preferably, described liquid storage pipe is made up of clear supple material；Described reaction tube is for having resilient transparent flexible pipe
Body.
Being preferably, the tail end of described reaction tube is provided with the adapter of band snap bar, and sets trocar sheath and be enclosed within outside adapter；
Described adapter cavity hollow, snap bar is solid.Break the snap bar of the adapter of disconnected reaction tube tail end, take out outside reaction tube
Sleeve pipe, the outer wall of extruding reaction pipe, liquid flows out from adapter port, oozes detection after can be used for reactant mixing.
Described can damaged ampoul tube can be one or more, preferably several.
In described gap and can filled chemical substance can be liquid in damaged ampoul tube, it is also possible to be solid.
The first device, the liquid separatnig valve in liquid storage pipe is two parts separated with sampling wiper sub bar, is separated with between the two
Gap.The advantage in this gap is to play the effect of another storage chamber, makes a liquid storage pipe inner region divide to store two simultaneously
Plant and the most different reagent.Additionally, porous membrane also can be placed in this gap, powdered chemical reagent is trapped in gap
In, or make saturated solution be filtered before flowing through sampling wiper sub bar, swab handle cavity will not be blocked.And the patent No.
ZL200510023792.5 uses be linked to be entirety damaged valve and sampling swab tubule can be unable to reach this effect.
The difference of the second device and the first device be to add can damaged ampoul tube, and the position of the needle of liquid separatnig valve
Put difference.The storage intracavity being positioned at liquid storage pipe and liquid separatnig valve formation from the needle of the first device liquid separatnig valve is different, and the second fills
The needle of the liquid separatnig valve in the liquid storage pipe put is positioned at the outside of the storage chamber solution of liquid storage pipe and liquid separatnig valve formation, liquid separatnig valve simultaneously
Being two parts separated with sampling wiper sub bar, there is certain gap in both in liquid storage pipe.Additionally, liquid separatnig valve and liquid storage pipe
Be placed with in the storage chamber formed can be damaged ampoul tube, ampoul tube is built with another chemical substance.The advantage of this design
It is: (1) liquid storage pipe can accommodate more than at least 3 kinds different reagent and (be placed with No. 1 reacting substance in ampoul tube, storage
The storage intracavity that liquid pipe and liquid separatnig valve are formed is filled with No. 2 reactant liquors, and liquid separatnig valve loads in liquid storage pipe, and needle is positioned at liquid storage pipe liquid
External body, forms closed system.Stator fixed sample swab thin bar, loads in liquid storage pipe, and and liquid separatnig valve have certain between
Gap, is placed with No. 3 reacting substances in gap).After being particularly well-suited to mixing, unstable needs is retained separately, and could mix before reaction
The reagent closed.(2) needle of liquid separatnig valve is positioned at the outside of storage chamber solution, gives to store intracavity and places the space of ampoul tube, with
Time will not be hindered by snap bar when racking ampoul tube, it is to avoid needle is fractureed during racking ampoul tube by mistake, too early
Liquid in release liquid storage pipe.The effect that this device reaches is that patent No. ZL200510023792.5 cannot realize.
Compared with prior art, the beneficial effects of the utility model are: this utility model simple in construction, give multiple examination
The space that agent subregion is placed, remixes use before reaction, can be continuously finished the collection of sample, process, react and detecting step, pole
The earth avoids sample contamination, easy to operate.This utility model device can be widely applied to the Pathogen test of clinical sample, microorganism
The fields such as cultivation, biochemical enzymes activity analysis, immunochemistry detection.
Accompanying drawing explanation
Fig. 1 a is the structural representation of liquid storage pipe in this utility model device.
Fig. 1 b is the structural representation of ampoul tube in this utility model device.
Fig. 1 c is the structural representation of liquid separatnig valve in this utility model device.
Fig. 1 d is the structural representation of porous membrane in this utility model device.
Fig. 1 e is the structural representation of sampling wiper sub bar stator in this utility model device.
Fig. 1 f is the structural representation of sampling wiper sub bar in this utility model device.
Fig. 1 g is the structural representation of reaction tube in this utility model device.
Fig. 1 h is the structural representation of this utility model embodiment 1 device.
Fig. 1 i is the structural representation of this utility model embodiment 2 device.
Fig. 2 a is that the tail end of the reaction tube of this utility model embodiment 3 connects the apparatus structure of adapter having band snap bar
Schematic diagram (effect: ooze detection after reactant mixing).
Fig. 2 b is that the tail end of the reaction tube of this utility model embodiment 4 connects the apparatus structure of adapter having band snap bar
Schematic diagram (effect: ooze detection after reactant mixing).
In figure, description of reference numerals is as follows:
1 is liquid storage pipe, and 2 is ampoul tube, and 3 is ampoule lid, and 4 is liquid separatnig valve, and 5 is needle, and 6 is porous membrane, and 7 is sampling
Swab handle stator, 8 is sampling wiper sub bar, and 9 is sampling wiper sub bar hole, and 10 is reaction tube, and 11 is adapter, and 12 is to fracture
Bar, 13 is trocar sheath, and A is gap, and B is cotton head.
Detailed description of the invention:
With embodiment, this utility model is described in detail below in conjunction with the accompanying drawings:
This utility model embodiment 1: the chromogenic reaction of enzyme-to-substrate
As shown in figure 1h, the present embodiment 1 device can be applicable to the chromogenic reaction of enzyme-to-substrate.Before reaction, enzyme-to-substrate needs
It is retained separately, the mixing when reaction.Meanwhile, in some cases, the lower instability of the substrate liquid of some enzyme temperature often, but its
Solid-state (powder) can stablize preservation at normal temperatures.Now, place containing enzyme in the storage chamber that liquid separatnig valve 4 and liquid storage pipe 1 are formed
Buffer, liquid separatnig valve 4 then places the corresponding substrate (powder or solid-state) of enzyme with the gap A of sampling wiper sub bar stator 7, and
Separate (porous membrane 6 is positioned at gap A, is close to sampling wiper sub bar 8 stator 7, sees Fig. 1 h) with porous membrane 6, prevent substrate
Fall in sampling wiper sub bar 8.During use, rotate the junction of liquid storage pipe 1 and reaction tube 10, take out sampling wiper sub bar 8 and sample, will
The cotton head B of sampling wiper sub bar 8 gathers sample.Sample collecting terminates, and sampling wiper sub bar 8 is turned back to (sampling swab in reaction tube 10
The cotton head B of bar 8 stretches into the inner chamber of reaction tube 10 and is positioned at reaction tube 10 end) and entangle firm with liquid storage pipe 1 and screw.Break disconnected liquid separatnig valve 4
Needle 5, extrude liquid storage pipe 1 outer wall, between enzyme buffer liquid stream enters between the valve pocket of liquid separatnig valve 4 and sampling wiper sub bar stator 7
Mix with zymolyte in gap A, more repeatedly extruding liquid storage pipe 1 outer wall, then the enzyme-to-substrate mixed can flow through sampling wiper sub bar 8 cavity
And react with the sample contents cotton head B from the outflow of sampling wiper sub bar hole 9.
This utility model embodiment 2: severe foster microorganism culturing
As shown in figure 1i, the present embodiment 2 device can be applicable to severe foster microorganism culturing.Clinical detection specimen is supported containing severe
Microorganism, such as Neisseria.This quasi-microorganism is the strictest to transporting the environmental requirement cultivated, it is desirable to contain in culture environment
The carbon dioxide of 5%-10%.Meanwhile, pattern detection terminates, and for avoiding sample contamination environment, can add antibacterial in device.
As shown in figure 1i, in ampoul tube 2, place antibacterial, place citric acid solution in liquid storage pipe 1, separate with liquid separatnig valve 4, liquid separatnig valve 4
And place powdered sodium carbonate in the gap A between sampling wiper sub bar stator 7, separate with porous membrane 6 that (porous membrane 6 is positioned at
In the A of gap, it is close to sampling wiper sub bar stator 7, sees Fig. 1 i).During use, take out sampling wiper sub bar 8 and sample, in retrieval device.
Fracture the needle 5 of liquid separatnig valve 4, with sodium carbonate hybrid reaction therein in extruding liquid storage pipe 1 outer wall release citric acid to gap A,
The carbon dioxide generated, through repeatedly extruding liquid storage pipe 1 outer wall, by the cavity of sampling wiper sub bar 8, mixes with sample.Make sample
It is stored in the environment of enough humidity and carbon dioxide, improves the activity of severe foster microorganism.After arriving test point, take out containing sample
This sampling wiper sub bar 8 coating is cultivated.Coating terminates, and by sampling wiper sub bar 8 retrieval device, racks ampoul tube 2 release sterilization
Agent, antibacterial and swab cotton head B effect, kill the thalline carried on sample, and whole device can be rationally without sterilization treatment
Dispose.
This utility model embodiment 3: sample pre-treatments
As shown in Figure 2 a, the present embodiment 3 device can apply to the pre-treatment of similar whole blood sample.As shown in Figure 2 a, liquid
State gold colloidal can be positioned in the storage chamber of liquid separatnig valve 4 and liquid storage pipe 1 formation, between liquid separatnig valve 4 and sampling wiper sub bar stator 7
Gap A in place cell cracking agent (such as TritonX-100), separate with porous membrane 6 that (porous membrane 6 is positioned at gap A
In, it is close to sampling wiper sub bar stator 7, sees Fig. 2 a).During use, rotate the junction of liquid storage pipe 1 and reaction tube 10, take out
Sampling wiper sub bar 8 is sampled.Sample collecting terminates, and turns back to sampling wiper sub bar 8 entangle firm in reaction tube 10 and with liquid storage pipe 1 screw.
Secondly, the needle 5 of disconnected liquid separatnig valve 4 is broken, between release liquid state colloid gold flows through between liquid separatnig valve 4 and sampling wiper sub bar stator 7
Gap A is reacted with cell cracking agent, and repeatedly extruding liquid storage pipe 1 outer wall, passes liquid through the cavity of sampling wiper sub bar 8 from sampling wiper
Sub bar hole 9 is flowed out and is mixed with the sample contents on cotton head B, thus processes sample.Sample treatment terminates, and breaks the tail of disconnected reaction tube 10
The snap bar 12 of the adapter 11 of end, takes out the trocar sheath 13 of reaction tube 10, and the outer wall of extruding reaction pipe 10, liquid is from adapter
11 ports ooze, and detect on Test paper card.
This utility model embodiment 4: prepare Antigen extraction reagent
As shown in Figure 2 b, the present embodiment 4 device can apply to similar acetic acid and the double-component reagent of sodium nitrite reaction.
After the mixing of both components, reaction generates nitrous acid.Nitrous acid is that the common extraction A race in microbe diagnosis field is streptococcic anti-
Original reagent, but nitrous acid instability, need preparation in time and utilize.Simultaneously as nitrous acid is acidic materials, upper test strips
Need between detection to regulate pH condition.Therefore, as shown in Figure 2 b, acetic acid can be positioned over the storage chamber that liquid separatnig valve 4 is formed with liquid storage pipe 1
In, acid-base neutralization reagent is positioned in ampoul tube 2, and stoppers ampoule lid 3.Between liquid separatnig valve 4 and sampling wiper sub bar stator 7
Gap A in place sodium nitrite, separate with porous membrane 6 that (porous membrane 6 is positioned at gap A, is close to sampling wiper sub bar and fixes
Sheet 7, is shown in Fig. 2 b).During use, rotate the junction of liquid storage pipe 1 and reaction tube 10, take out sampling wiper sub bar 8 and sample.Sample is adopted
Collection terminates, and turns back to sampling wiper sub bar 8 entangle firm in reaction tube 10 and with liquid storage pipe 1 screw.Secondly, the needle of disconnected liquid separatnig valve 4 is broken
5, the gap A that releasing liquid flows through between liquid separatnig valve 4 and sampling wiper sub bar stator 7 reacts with sodium nitrite, repeatedly extrudes storage
Liquid pipe 1 outer wall, the cavity passing liquid through sampling wiper sub bar 8 flows out mixed with the sample contents cotton head B from sampling wiper sub bar hole 9
Close, thus extract sample antigen.Finally, discharging acid-base neutralization reagent by broken ampoul tube 2, acid-base neutralization reagent arrives cotton head B's
Position neutralization reaction product.Extraction terminates, and breaks the snap bar 12 of the adapter 11 of the tail end of disconnected reaction tube 10, takes out reaction tube
The trocar sheath 13 of 10, the outer wall of extruding reaction pipe 10, liquid flows out from adapter 11 port.Can be at A race streptococcus Test paper
On card, detection or external small tubes collect antigen.
Claims (9)
1. one kind collect sampling, react, be detected as one device, it is characterised in that this device includes: liquid storage pipe (1), liquid separatnig valve
(4), stator (7), sampling wiper sub bar (8), reaction tube (10)；Liquid separatnig valve (4) is provided with broken needle (5)；Stator
(7) sampling wiper sub bar (8) is connected；Liquid separatnig valve (4) loads liquid storage pipe (1) inner chamber, and what liquid storage pipe (1) and liquid separatnig valve (4) were formed deposits
Storage chamber is positioned at described storage intracavity built with solution, the needle (5) of liquid separatnig valve (4)；Stator (7) loads in liquid storage pipe (1), with
It is provided with gap (A) between liquid separatnig valve (4), in gap (A), places chemical substance；The opening of liquid storage pipe (1) puts reaction tube
(10)。
Device the most according to claim 1, it is characterised in that be additionally provided with porous membrane (6) in described gap (A).
Device the most according to claim 1, it is characterised in that: one end of described sampling wiper sub bar (8) be provided with aseptic cotton carrier,
Inoculating loop or spiral sampling cone, the other end inserts in the cavity of stator (7).
Device the most according to claim 1, it is characterised in that: the tail end of described reaction tube (10) is provided with band snap bar (12)
Adapter (11), and set trocar sheath (13) and be enclosed within the outside of adapter (11)；Described adapter (11) cavity hollow, snap bar
(12) it is solid.
5. one kind collects sampling, reacts, is detected as the device of one, it is characterised in that this device includes: liquid storage pipe (1), can be damaged
Ampoul tube (2), liquid separatnig valve (4), stator (7), sampling wiper sub bar (8), reaction tube (10)；Liquid separatnig valve (4) is provided with broken
Needle (5)；Stator (7) connects sampling wiper sub bar (8)；Liquid separatnig valve (4) load liquid storage pipe (1) inner chamber, liquid storage pipe (1) and divide
The storage intracavity that liquid valve (4) is formed is positioned at outside described storage chamber equipped with solution, the needle (5) of liquid separatnig valve (4)；Described can damaged pacify
Small jar pipe (2) is preinstalled with chemical substance, and loads described storage intracavity；Stator (7) loads in liquid storage pipe (1), with liquid separatnig valve (4)
Between be provided with gap (A), place chemical substance in gap (A)；The opening of liquid storage pipe (1) puts reaction tube (10).
Device the most according to claim 5, it is characterised in that: it is additionally provided with porous membrane (6) in described gap (A).
Device the most according to claim 5, it is characterised in that: one end of described sampling wiper sub bar (8) be provided with aseptic cotton carrier,
Inoculating loop or spiral sampling cone, the other end inserts in the cavity of stator (7).
Device the most according to claim 5, it is characterised in that: the tail end of described reaction tube (10) is provided with band snap bar (12)
Adapter (11), and setting trocar sheath (13), to be enclosed within adapter (11) outside；Described adapter (11) cavity hollow, snap bar
(12) it is solid.
Device the most according to claim 5, it is characterised in that: described can damaged ampoul tube (2) be multiple.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620650350.7U CN205826329U (en) | 2016-06-27 | 2016-06-27 | Collection is sampled, reacts, is detected as device integrally |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620650350.7U CN205826329U (en) | 2016-06-27 | 2016-06-27 | Collection is sampled, reacts, is detected as device integrally |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205826329U true CN205826329U (en) | 2016-12-21 |
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ID=58146993
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620650350.7U Active CN205826329U (en) | 2016-06-27 | 2016-06-27 | Collection is sampled, reacts, is detected as device integrally |
Country Status (1)
| Country | Link |
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| CN (1) | CN205826329U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106053121A (en) * | 2016-06-27 | 2016-10-26 | 益思美诠生物科技(上海)有限公司 | Device integrating sampling, reaction and detection |
| CN112680338A (en) * | 2021-01-25 | 2021-04-20 | 塔里木大学 | Inoculation and sampling device for walnut endophytic bacterium HB1310 fermented cotton stalk hydrolyzed sugar liquid oil production fermentation broth |
-
2016
- 2016-06-27 CN CN201620650350.7U patent/CN205826329U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106053121A (en) * | 2016-06-27 | 2016-10-26 | 益思美诠生物科技(上海)有限公司 | Device integrating sampling, reaction and detection |
| CN112680338A (en) * | 2021-01-25 | 2021-04-20 | 塔里木大学 | Inoculation and sampling device for walnut endophytic bacterium HB1310 fermented cotton stalk hydrolyzed sugar liquid oil production fermentation broth |
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| C14 | Grant of patent or utility model | ||
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