CN101555516A - Candida albicans rapid identification and cultivation susceptibility test kit and test method - Google Patents
Candida albicans rapid identification and cultivation susceptibility test kit and test method Download PDFInfo
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- CN101555516A CN101555516A CNA2009100225517A CN200910022551A CN101555516A CN 101555516 A CN101555516 A CN 101555516A CN A2009100225517 A CNA2009100225517 A CN A2009100225517A CN 200910022551 A CN200910022551 A CN 200910022551A CN 101555516 A CN101555516 A CN 101555516A
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Abstract
The invention discloses a candida albicans rapid identification and cultivation susceptibility test kit which comprises a kit and a susceptibility panel, wherein, the kit comprises a box body which is internally provided with ultrapure water reagent, paraffin oil reagent, physiological saline reagent and candida albicans rapid identification culture medium; the susceptibility panel includes a panel body and a panel cover, wherein, the panel body is provided with a negative test hole and a positive test hole, a plurality of drug property comparison holes are arranged correspondingly to the negative test hole and the positive test hole, and the drug property comparison holes corresponding to the positive test hole is internally provided with test medicine. The invention also discloses a test method which comprises: culture fluid is firstly prepared and then added into the negative test hole to extract pathological change solution, and the pathological change solution is added into the culture fluid to obtain mixed solution; the mixed solution is added into the positive test hole and the drug property comparison holes to be cultured, and then the result is obtained. The candida albicans rapid identification and cultivation susceptibility test kit and the test method are simple in operation and short in testing period.
Description
Technical field
The invention belongs to the vitro diagnostic techniques field, be specifically related to a kind of candida albicans rapid identification and cultivation susceptibility test kit, the invention still further relates to the method for utilizing this test kit to detect.
Background technology
Candida albicans is a kind of endogenous fungi, and the respiratory tract, vagina etc. that can be present in healthy people are located, and belong to conditioned pathogen.Monilial vaginitis is a kind of common gynecological disease, is the colpitis that is caused by Candida albicans, and it is a principal character with pruritus vulvae, cusalgia, bean dregs sample leukorrhea.
What be used in the market that the evaluation of Candida albicans cultivates mainly is culture method, and the drug sensitive test product does not then almost have.Common diagnostic method is, cultivate about 2～3 days by flat board after, observe colonial morphology, be defined as after the Candida albicans, picking colony is analyzed resistance with paper disk method again, diagnosis is finished in front and back approximately needs 10 days time.Operator are had higher requirements, and waste time and energy, be not suitable for the requirement of clinical quick diagnosis.
Summary of the invention
The purpose of this invention is to provide a kind of candida albicans rapid identification and cultivation susceptibility test kit, solved the problem that wastes time and energy that existing diagnostic method exists.
Another object of the present invention provides the method for utilizing this susceptibility test kit to detect.
The technical solution adopted in the present invention is, a kind of candida albicans rapid identification and cultivation susceptibility test kit, comprise test kit and drug sensitive plate, test kit comprises box body, be placed with ultrapure water reagent, paraffin oil reagent, physiological saline reagent and Candida albicans Rapid identification substratum in the box body, drug sensitive plate comprises plate body and Ban Gai, plate body is provided with negative hole and the positive detection hole detected, detect in the property of medicine contrast hole that hole and positive detection hole be provided with several property of medicine contrast holes and positive detection hole correspondence corresponding to feminine gender detection of drugs is housed.
Another technical scheme of the present invention is, the method for utilizing this susceptibility test kit to detect is carried out according to the following steps,
Step 1: get ultrapure water reagent and Candida albicans Rapid identification substratum, ultrapure water reagent is joined in the Candida albicans Rapid identification substratum, after the dissolving, obtain nutrient solution fully;
Step 2: get nutrient solution 50～100 μ l that the step obtains, the feminine gender that joins on the plate body detects in the hole;
Step 3: get a physiological saline reagent, adsorb physiological saline, at the twisted several of diseased region with cotton swab, take out cotton swab, place immediately in the nutrient solution that step 1 obtains, stir cotton swab and extract wherein liquid, mix obtaining mixed nutrient solution with sample injector piping and druming or with the oscillator concussion;
Step 4: get the mixed nutrient solution that the step obtains, join respectively in the positive detection hole and property of medicine contrast hole on the plate body, add 50～100 μ l in each hole;
Step 5: get paraffin oil reagent, Dropwise 50 μ l paraffin oil in each hole on plate body covers the plate lid, places drug sensitive plate 35～37 ℃ of incubators to cultivate;
Observe in step 6:6～24 hour and read the result, examination criteria is as follows:
If negative detection hole nondiscoloration, the nondiscoloration of positive detection hole then shows no albicans growth, and remaining hole does not make a decision;
If negative detection hole variable color then shows pollution, invalidate the test;
If negative detection hole nondiscoloration, the variable color of positive detection hole shows adularescent candidiasis in the sample, judges remaining each hole result;
A: and the property of medicine contrast hole nondiscoloration of the property of medicine contrast hole nondiscoloration of negative detection hole correspondence and positive detection hole correspondence, expression is to the medicaments insensitive of this property of medicine contrast hole correspondence;
B: and the property of medicine contrast hole variable color of the property of medicine contrast hole nondiscoloration of negative detection hole correspondence and positive detection hole correspondence, expression is to the medium sensitivity of medicine of this property of medicine contrast hole correspondence;
C: and the property of medicine contrast hole variable color of the property of medicine contrast hole variable color of negative detection hole correspondence and positive detection hole correspondence, expression is insensitive to the medicine of this property of medicine contrast hole correspondence.
The invention has the beneficial effects as follows,
(1) improves the Candida albicans substratum, add quick growth factor therein, make the cultivation of Candida albicans break through about 7 days time limitation of culture-based method, can finish the cultivation of Candida albicans in 6～24 hours, its composition mainly comprises peptone, glucose, yeast extract, indicator, quick growth factor etc.In addition, substratum is processed as dry powder, helps the prolonged preservation of product, with using after the supporting ultrapure water dissolving.Simultaneously supporting have a ultrapure water, both uses for convenience, and what also cause for fear of the difference of different areas water quality can not expected result, has guaranteed product stability of the present utility model on to greatest extent.Also be equipped with stroke-physiological saline solution, be used for moistening cotton swab when gathering sample, in the sample short period of time that can guarantee to collect can be dead because of the change of environment, guarantee the accuracy of sample cultivation results.
(2) the method principle that medicine sensitive detecting plate adopted is to improve to form on the basis of the trace liquid diluting method principle of the NCCLS of international standard.Conventional trace liquid diluting method is that the bacterium colony through the screening of mirror mirror is carried out enlarged culturing, more cultured nutrient solution is joined in the good antibiotic concentration gradient of prior dilution, cultivates observations.Because this method operation steps is more, the drug sensitive test of finishing a case approximately needs about 7～8 days time, and culture success ratio is lower, and needs the dedicated experiments personnel to operate.Drug sensitive plate of the present invention comprises susceptibility plate body and Ban Gai, first row are provided with control wells in the left side of drug sensitive plate, comprise positive and negative control reacting hole, the every row reacting hole that begins from secondary series all corresponding to a kind of antibiotic medicine commonly used at present, comprising: itraconazole, nystatin, amphotericin, flucytosine, miconazole, KETOKONAZOL, econazole, clotrimazole, fluconazole, Lamisil, Mepartricin, Ciclopirox Olamine, Caspofungin, trichomycin.And every kind of microbiotic all has corresponding two concentration-response holes of height on drug sensitive plate, and all microbiotic all adopt the heat bag to be wrapped in advance to be advanced in the drug sensitive plate reacting hole by method.Each susceptibility plate body all has a drug sensitive plate lid corresponding, can prevent that extraneous dust or other foreign material from falling into the susceptibility hole or scratching the drug sensitive reaction hole, influences the accuracy of drug sensitive test.Be equipped with the paraffin oil after the sterilization simultaneously, be used in the drug sensitive test process, sealing drug sensitive reaction hole prevents the volatilization of liquid to greatest extent, influences result's judgement.
Description of drawings
Fig. 1 is the structural representation of test kit in the susceptibility test kit of the present invention;
Fig. 2 is the structural representation of drug sensitive plate in the susceptibility test kit of the present invention;
Fig. 3 is the structural representation of plate lid in the susceptibility test kit of the present invention.
Among the figure, 1. box body, 2. ultrapure water reagent, 3. paraffin oil reagent, 4. physiological saline reagent, 5. Candida albicans Rapid identification substratum, the 6. negative hole of detecting, 7. positive detection hole, 8. property of medicine contrast hole, 9. plate body, 10. plate lid.
Embodiment
The present invention is described in detail below in conjunction with the drawings and specific embodiments.
Candida albicans rapid identification and cultivation susceptibility test kit of the present invention comprises test kit part and drug sensitive plate part, the structure of test kit as shown in Figure 1, comprise box body 1, be placed with two ultrapure water reagent 2 in the box body 1, a paraffin oil reagent 3, a physiological saline reagent 4 and a Candida albicans Rapid identification substratum 5, the structure of drug sensitive plate as shown in Figures 2 and 3, comprise plate body 9 and plate lid 10, plate body 9 is provided with negative hole 6 and the positive detection hole 7 detected, detect in the delegation property of medicine contrast hole 8 that hole 6 and positive detection hole 7 be provided with two row property of medicine contrast holes 8 and positive detection hole 7 correspondences corresponding to feminine gender detection of drugs is housed.
The method of utilizing susceptibility test kit of the present invention to detect, specifically implement according to following steps:
Step 1: get a ultrapure water reagent 2 and a Candida albicans Rapid identification substratum 5, ultrapure water is joined in the Candida albicans Rapid identification substratum 5, after the dissolving, obtain nutrient solution fully;
Step 2: get nutrient solution 50～100 μ l that the step obtains, the feminine gender that joins on the drug sensitive plate detects in the hole 6;
Step 3: get a physiological saline reagent 4, adsorb physiological saline with cotton swab, at the twisted several of diseased region, take out cotton swab, place immediately in the nutrient solution that step 1 obtains, stir cotton swab for several times, extract cotton swab, squeeze out wherein liquid as far as possible, take out cotton swab facing to the bottle wall, shake mixing for several times or with oscillator with sample injector piping and druming, obtain mixed nutrient solution;
Step 4: get mixed nutrient solution 50～100 μ l that the step obtains, join respectively in the positive detection hole 7 and property of medicine contrast hole 8 on the drug sensitive plate;
Step 5: get paraffin oil reagent 3, Dropwise 50 μ l paraffin oil in each hole on drug sensitive plate covers plate lid 10, places drug sensitive plate 35～37 ℃ of incubators to cultivate;
Observe in step 6:6～24 hour and read the result, interpretation standard is as follows:
(1) if feminine gender detects hole 6 nondiscolorations, 7 nondiscolorations of positive detection hole then show no albicans growth, and remaining hole does not make a decision;
(2) if negative detection hole 6 variable colors then show pollution, invalidate the test;
(3) if feminine gender detects hole 6 nondiscolorations, 7 variable colors of positive detection hole show adularescent candidiasis in the sample, judge remaining each hole result;
A: and negative property of medicine contrast hole 8 nondiscolorations of hole 6 correspondences and property of medicine contrast hole 8 nondiscolorations of positive detection hole 7 correspondences, the medicaments insensitive that expression is corresponding to these row of detecting;
B: and negative property of medicine contrast hole 8 nondiscolorations of hole 6 correspondences and property of medicine contrast hole 8 variable colors of positive detection hole 7 correspondences, the medium sensitivity of medicine that expression is corresponding to these row of detecting;
C: and property of medicine contrast hole 8 variable colors of property of medicine contrast hole 8 variable colors of negative detection hole 6 correspondences and positive detection hole 7 correspondences, expression is insensitive to the medicine of this row correspondence.
Embodiment
Get a ultrapure water reagent 2 and a Candida albicans Rapid identification substratum 5, the 1.5ml ultrapure water is joined in the Candida albicans Rapid identification substratum 5, after the dissolving, obtain nutrient solution fully; Get the nutrient solution 50 μ l that the step obtains, the feminine gender that joins on the drug sensitive plate detects in the hole 6; Get a physiological saline reagent 4, adsorb physiological saline with cotton swab, at the twisted several of diseased region, take out cotton swab, place immediately in the nutrient solution that step 1 obtains, stir cotton swab for several times, extract cotton swab, squeeze out wherein liquid as far as possible, take out cotton swab facing to the bottle wall, shake mixing for several times or with oscillator with sample injector piping and druming, obtain mixed nutrient solution; Get the mixed nutrient solution 50 μ l that the step obtains, join respectively in the positive detection hole 7 and property of medicine contrast hole 8 on the drug sensitive plate; Get paraffin oil reagent 3, Dropwise 50 μ l paraffin oil in each hole on drug sensitive plate covers plate lid 10, places drug sensitive plate 35～37 ℃ of incubators to cultivate; Take out after 6 hours and read the result,
Wherein drug sensitive plate medicine english abbreviation and Chinese contrast:
| Medicine name | Itraconazole | Nystatin | Amphotericin | Flucytosine | Miconazole | KETOKONAZOL | Econazole | Clotrimazole | Fluconazole | Lamisil | Mepartricin | Ciclopirox Olamine | Caspofungin | Trichomycin |
| English abbreviation | ITR | NYS | AMP | FLU | MIC | KET | ECO | KLO | FHC | LAM | MEP | CIC | CAS | TRI |
Detected result is as follows:
It is redness or grass green that hole 6 is detected corresponding to feminine gender in ITR medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the ITR medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of ITR medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the ITR medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in NYS medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the NYS medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of NYS medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the NYS medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in AMP medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the AMP medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of AMP medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the AMP medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in FLU medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the FLU medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of FLU medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the FLU medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in MIC medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the MIC medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of MIC medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the MIC medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in KET medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the KET medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of KET medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the KET medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in ECO medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the ECO medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of ECO medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the ECO medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in KLO medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the KLO medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of KLO medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the KLO medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in FHC medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the FHC medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of FHC medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the FHC medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in LAM medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the LAM medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of LAM medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the LAM medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in MEP medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the MEP medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of MEP medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the MEP medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in LAM medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the LAM medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of LAM medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the LAM medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in CIC medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the CIC medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of CIC medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the CIC medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in CAS medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the CAS medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of CAS medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the CAS medicine;
It is redness or grass green that hole 6 is detected corresponding to feminine gender in TRI medicine contrast hole 8, is redness or grass green corresponding to positive detection hole 7, shows that this Candida albicans is to the TRI medicaments insensitive; It is redness or grass green that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is to the medium sensitivity of TRI medicine; It is yellow that hole 6 is detected corresponding to feminine gender in medicine contrast hole 8, is yellow corresponding to positive detection hole 7, shows that this Candida albicans is insensitive to the TRI medicine.
Candida albicans rapid identification and cultivation susceptibility test kit of the present invention and detection method, simple to operate and sense cycle weak point.
Claims (2)
1. candida albicans rapid identification and cultivation susceptibility test kit, it is characterized in that, comprise test kit and drug sensitive plate, described test kit comprises box body (1), be placed with ultrapure water reagent (2) in the box body (1), paraffin oil reagent (3), physiological saline reagent (4) and Candida albicans Rapid identification substratum (5), described drug sensitive plate comprises plate body (9) He Bangai (10), plate body (9) is provided with negative hole (6) and positive detection hole (7) detected, detecting hole (6) and positive detection hole (7) corresponding to feminine gender is provided with several property of medicine contrast holes (8) and positive detection hole (7) corresponding property of medicine and contrasts in the hole (8) detection of drugs is housed.
2. method of utilizing the described candida albicans rapid identification and cultivation susceptibility test kit of claim 1 to detect, it is characterized in that, comprise test kit and drug sensitive plate, described test kit comprises box body (1), be placed with ultrapure water reagent (2) in the box body (1), paraffin oil reagent (3), physiological saline reagent (4) and Candida albicans Rapid identification substratum (5), described drug sensitive plate comprises plate body (9) He Bangai (10), plate body (9) is provided with negative hole (6) and positive detection hole (7) detected, be provided with several property of medicine contrast holes (8) corresponding to feminine gender detection hole (6) and positive detection hole (7), with in positive detection hole (7) corresponding property of medicine contrast hole (8) detection of drugs is housed
Specifically implement according to following steps,
Step 1: get ultrapure water reagent (2) and Candida albicans Rapid identification substratum (5), ultrapure water reagent (2) is joined in the Candida albicans Rapid identification substratum (5), after the dissolving, obtain nutrient solution fully;
Step 2: get nutrient solution 50～100 μ l that the step obtains, the feminine gender that joins on the plate body (9) detects in the hole (6);
Step 3: get a physiological saline reagent (4), adsorb physiological saline with cotton swab, at the twisted several of diseased region, take out cotton swab, place immediately in the nutrient solution that step 1 obtains, stir cotton swab and extract wherein liquid, mix obtaining mixed nutrient solution with sample injector piping and druming or with the oscillator concussion;
Step 4: get the mixed nutrient solution that the step obtains, join respectively in the positive detection hole (7) and property of medicine contrast hole (8) on the plate body (9), add 50～100 μ l in each hole;
Step 5: get paraffin oil reagent (3), Dropwise 50 μ l paraffin oil in each hole on plate body (9) covers plate lid (10), places drug sensitive plate 35～37 ℃ of incubators to cultivate;
Observe in step 6:6～24 hour and read the result, examination criteria is as follows:
If negative detection hole (6) nondiscoloration, positive detection hole (7) nondiscoloration then shows no albicans growth, and remaining hole does not make a decision;
If negative detection hole (6) variable color then shows pollution, invalidate the test;
If negative detection hole (6) nondiscoloration, positive detection hole (7) variable color shows adularescent candidiasis in the sample, judges remaining each hole result;
A: and negative corresponding property of medicine contrast hole (8) nondiscoloration in hole (6) and positive detection hole (7) corresponding property of medicine contrast hole (8) nondiscoloration, the medicaments insensitive that expression is corresponding to this property of medicine contrast hole (8) of detecting;
B: and negative corresponding property of medicine contrast hole (8) nondiscoloration in hole (6) and positive detection hole (7) corresponding property of medicine contrast hole (8) variable color, the medium sensitivity of medicine that expression is corresponding to this property of medicine contrast hole (8) of detecting;
C: and negative property of medicine contrast hole (8) variable color and positive detection hole (7) corresponding property of medicine contrast hole (8) variable color that detects hole (6) correspondence, it is insensitive that expression contrasts the corresponding medicine in hole (8) to this property of medicine.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103013822A (en) * | 2012-12-24 | 2013-04-03 | 山东省海水养殖研究所 | Fast drug sensitivity detection kit for aquatic product vibrio |
| CN106755279A (en) * | 2017-03-23 | 2017-05-31 | 合肥百盛园生物药业有限公司 | Nutrient solution, kit and detection method for detection bacterium vaginitis |
| CN106916728A (en) * | 2017-04-18 | 2017-07-04 | 朱红 | A kind of magnetic stirring Analysis of Drug Susceptibility instrument and matched reagent box |
| CN106939329A (en) * | 2017-03-23 | 2017-07-11 | 合肥百盛园生物药业有限公司 | A kind of nutrient solution, kit and detection method for being used to detect colpomycosis |
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2009
- 2009-05-15 CN CNA2009100225517A patent/CN101555516A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103013822A (en) * | 2012-12-24 | 2013-04-03 | 山东省海水养殖研究所 | Fast drug sensitivity detection kit for aquatic product vibrio |
| CN103013822B (en) * | 2012-12-24 | 2014-07-16 | 山东省海洋生物研究院 | Fast drug sensitivity detection kit for aquatic product vibrio |
| CN106755279A (en) * | 2017-03-23 | 2017-05-31 | 合肥百盛园生物药业有限公司 | Nutrient solution, kit and detection method for detection bacterium vaginitis |
| CN106939329A (en) * | 2017-03-23 | 2017-07-11 | 合肥百盛园生物药业有限公司 | A kind of nutrient solution, kit and detection method for being used to detect colpomycosis |
| CN106916728A (en) * | 2017-04-18 | 2017-07-04 | 朱红 | A kind of magnetic stirring Analysis of Drug Susceptibility instrument and matched reagent box |
| CN106916728B (en) * | 2017-04-18 | 2023-10-27 | 朱红 | Magnetic stirring type drug sensitive analytical instrument and matched kit |
| CN109622077A (en) * | 2018-12-10 | 2019-04-16 | 郑州安图生物工程股份有限公司 | Self-enclosed reaction orifice plate and preparation method thereof when a kind of incubation |
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