CN106916728B - Magnetic stirring type drug sensitive analytical instrument and matched kit - Google Patents
Magnetic stirring type drug sensitive analytical instrument and matched kit Download PDFInfo
- Publication number
- CN106916728B CN106916728B CN201710254526.6A CN201710254526A CN106916728B CN 106916728 B CN106916728 B CN 106916728B CN 201710254526 A CN201710254526 A CN 201710254526A CN 106916728 B CN106916728 B CN 106916728B
- Authority
- CN
- China
- Prior art keywords
- stirring
- holes
- kit
- detection
- drug sensitive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000003814 drug Substances 0.000 title claims abstract description 113
- 229940079593 drug Drugs 0.000 title claims abstract description 92
- 238000003760 magnetic stirring Methods 0.000 title claims abstract description 15
- 238000003756 stirring Methods 0.000 claims abstract description 92
- 238000001514 detection method Methods 0.000 claims abstract description 55
- 238000000034 method Methods 0.000 claims abstract description 44
- 230000008569 process Effects 0.000 claims abstract description 28
- 244000005700 microbiome Species 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 230000009471 action Effects 0.000 claims abstract description 7
- 230000002829 reductive effect Effects 0.000 claims abstract description 6
- 238000009413 insulation Methods 0.000 claims description 22
- 238000011896 sensitive detection Methods 0.000 claims description 21
- 239000003153 chemical reaction reagent Substances 0.000 claims description 20
- 238000012545 processing Methods 0.000 claims description 19
- 230000033001 locomotion Effects 0.000 claims description 18
- 239000002184 metal Substances 0.000 claims description 17
- 238000005485 electric heating Methods 0.000 claims description 15
- 238000003491 array Methods 0.000 claims description 14
- 238000006073 displacement reaction Methods 0.000 claims description 14
- 230000003287 optical effect Effects 0.000 claims description 10
- 229920000742 Cotton Polymers 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 7
- 230000008878 coupling Effects 0.000 claims description 6
- 238000010168 coupling process Methods 0.000 claims description 6
- 238000005859 coupling reaction Methods 0.000 claims description 6
- 239000013641 positive control Substances 0.000 claims description 6
- 239000003242 anti bacterial agent Substances 0.000 claims description 5
- 229940088710 antibiotic agent Drugs 0.000 claims description 5
- 230000009286 beneficial effect Effects 0.000 claims description 4
- 238000007599 discharging Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 230000000813 microbial effect Effects 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 230000000903 blocking effect Effects 0.000 claims description 2
- 230000008859 change Effects 0.000 claims description 2
- 238000004891 communication Methods 0.000 claims description 2
- 230000003247 decreasing effect Effects 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 238000005286 illumination Methods 0.000 claims description 2
- 230000035945 sensitivity Effects 0.000 abstract description 9
- 238000012258 culturing Methods 0.000 abstract description 6
- 238000010206 sensitivity analysis Methods 0.000 abstract description 5
- 206010059866 Drug resistance Diseases 0.000 abstract description 3
- 230000036961 partial effect Effects 0.000 description 9
- 238000010586 diagram Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
Abstract
The invention relates to the technical field of drug sensitivity analysis, in particular to a magnetic stirring type drug sensitivity analysis instrument and a matched kit. The invention is matched with a microcomputer for use, has simple operation, short drug sensitivity time and high result accuracy. The drug resistance of the microorganism can be judged by a final value method, the growth state of the microorganism can be monitored in the culture process, the stirring function is increased in the culture process, the propagation speed of the drug-resistant microorganism is accelerated, the time of the whole drug-sensitive process is shortened, and the steps of manual operation are reduced. In the process of culturing microorganisms, the stirring motor rotates to drive the magnet to rotate, the stirrer rotates under the action of the rotating magnetic field, and medicines, microorganism liquid and air in the medicine-sensitive stirring hole are fully mixed. The propagation speed of drug-resistant microorganisms of the medicine is accelerated, and the process of drug sensitivity is shortened. After a period of cultivation, the moving device moves to the detecting device with the kit, and the growth curve of the microorganism is finally obtained through repeated cultivation and detection.
Description
Technical Field
The invention relates to the technical field of drug sensitivity analysis, in particular to a magnetic stirring type drug sensitivity analysis instrument and a matched kit.
Background
As microbial resistance becomes more severe, infectious diseases require accurate identification of pathogenic bacteria species and testing of their in vitro antimicrobial drug sensitivity (AST), providing a clinical medication reference. Currently, clinic relies mainly on manual methods or commercial analysis systems to conduct the tests. The traditional manual method and commercial analysis system generally adopt a final value method, dilute the bacterial colony by a certain multiple, add into the antibiotic reagent box coated with different types and concentrations, put into the incubator for culturing for 24 hours, visually observe the turbidity of the liquid in the small hole of the reagent box, or judge the drug resistance of the microorganism by an instrument interpretation mode. The operation steps are numerous. And the whole culture process is always in a static state, which is not beneficial to the full contact of bacteria with medicines and nutrient components and the supply of oxygen in the culture process. The whole process needs to take a long time, and the requirement of clinic on rapid detection of the project cannot be met.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the invention provides a magnetic stirring type drug sensitivity analysis instrument and a matched kit, which can detect by a final value method, can monitor in the culture process, and can increase the stirring function in the culture process, so that microorganisms are fully contacted with drugs, nutritional ingredients and oxygen, the time of the drug sensitivity process is shortened, and the steps of manual operation are reduced.
(II) technical scheme
In order to achieve the above purpose, the invention is realized by the following technical scheme:
a magnetic stirring type drug sensitive analytical instrument, which is characterized in that: comprises a constant temperature culture device, a stirring device, a motion device and a detection device; the constant temperature culture device comprises an electric heating plate, a fan, a thermosensitive element and an insulation can, wherein the thermosensitive element is used for detecting the temperature of air in the insulation can and converting the temperature value into a corresponding voltage value, the stirring device comprises a stirring motor, a magnet and a stirrer, the magnet is connected to a rotating shaft of the stirring motor through an adapter, the stirrer is located in a stirring hole of a matched kit, the moving device comprises a moving motor, a displacement platform, a first photoelectric sensor, a second photoelectric sensor, a third photoelectric sensor, a fourth photoelectric sensor and a kit tray, the moving motor and the displacement platform are mounted on the metal plate and are on the same straight line, a shaft coupling is fixedly connected to the motor shaft through screws, and the other end of the shaft coupling is fixedly connected to the rotating shaft of the displacement platform through screws, and the detecting device comprises a white light LED array, an optical collimating component and a photosensitive array.
Preferably, the insulation can is divided into a bottom plate and a box body, the bottom plate is composed of three layers of different materials of a metal plate, insulation cotton and a plastic plate, the three layers of different materials are placed in an overlapping mode, the insulation cotton is located between the metal plate and the plastic plate, the box body is composed of the insulation cotton and the plastic plate, the metal plate is high in strength, the insulation can is supported by the inner structure, heat loss in the insulation can is reduced by the insulation cotton, a fan hole is reserved at the rear side of the box body, an electric heating plate and a fan are fixed on the metal plate through a fixing support and are located on the same straight line, the fan faces the fan hole at the rear side of the box body of the insulation can, and the electric heating plate, the fan and a thermosensitive element are connected to a signal control processing unit through respective control circuits.
Preferably, the preset temperature value in the internal register of the signal control processing unit is a temperature range, the fan is in a continuous working state, the electric heating plate is in an intermittent working state, the temperature detected by the thermosensitive element is higher than the preset temperature value, the electric heating plate does not work, the fan brings cold air outside the insulation box into the box body, the temperature is accelerated to be reduced, the detected temperature is lower than the preset temperature value, the electric heating plate works, the fan brings heat of the heating plate into the box body, the air inside the insulation box is stirred, and the cold air and the hot air are uniformly mixed.
Preferably, a stirrer is arranged in the culture hole of each matched kit, the number of stirring motors and magnets is determined by the number of columns of drug-resistant stirring holes of the matched kit, the stirring motors rotate to drive the magnets to rotate, the stirrer positioned right above the magnets rotates under the action of a rotating magnetic field, and drugs, microbial liquid, air and nutritional ingredients in the drug-resistant stirring holes are fully mixed to accelerate the propagation speed of drug-resistant microorganisms of the drugs and shorten the drug-resistant process.
Preferably, one end, close to the motor, of the displacement platform rotating shaft is connected with a kit tray through a conversion piece in a screw fixed manner, a plurality of kit placement areas are formed in the kit tray, the sizes of the kit placement areas are determined according to the sizes of the kits, a plurality of round holes are reserved on the panel of the kit placement areas and distributed in an array manner, and the number, the sizes of the round holes and the distance between the small holes are consistent with those of the drug sensitive detection holes in the kits.
Preferably, one side of the kit tray is provided with a row of dentate protrusions, the number of the dentate protrusions is consistent with the number of the detection hole rows in the kit, the distance between the dentate protrusions is consistent with the distance between the centers of the detection holes in the adjacent rows in the kit, the other side of the kit tray is provided with 2 dentate protrusions which are respectively a first dentate protrusion and a second dentate protrusion, and the distance between the first dentate protrusion and the second dentate protrusion is consistent with the distance between the drug-sensitive stirring holes in the first row and the drug-sensitive stirring holes in the last row in the kit.
Preferably, the first photoelectric sensor, the second photoelectric sensor, the third photoelectric sensor and the fourth photoelectric sensor are opposite-emitting photoelectric sensors, the first photoelectric sensor is blocked by an opaque object, signals at the output end change, the first photoelectric sensor is fixed on a first fixed support on one side of a metal plate, the second photoelectric sensor, the third photoelectric sensor and the fourth photoelectric sensor are respectively fixed on a second fixed support, a third fixed support and a fourth fixed support on the other side of the metal plate, during culture, the fourth photoelectric sensor is blocked by a second tooth-shaped protrusion, the electric level changes, the stirring device stirs substances in the first row of medicine-sensitive stirring holes, after a period of time, the motion motor rotates for a certain step number, the stirring device stirs the substances in the second row of medicine-sensitive stirring holes, sequentially stirs the third row and the fourth row of medicine-sensitive stirring holes downwards, when the electric level changes, all the substances in the medicine-sensitive stirring holes can be stirred, and simultaneously the motion motor moves reversely to sequentially stir the first row and the second row of reciprocal in turn downwards, and the purpose of stirring the substances in all medicine-sensitive stirring holes is achieved by using least hardware.
Preferably, after the microorganism is cultured for a period of time, the moving device moves to a detection position with the reagent box, the third tooth-shaped protrusion blocks the first photoelectric sensor, the first row is detected, the moving motor rotates, the fourth tooth-shaped protrusion blocks the first photoelectric sensor, the second row is detected, the third row and the fourth row are sequentially detected to be downwards, the repeated culture and detection are performed, when the reagent box is placed and taken out, the instruction is input through the input end of the microcomputer, the signal processing control unit receives the instruction and then controls the moving motor to push the reagent box tray to move towards the discharging direction, the first tooth-shaped protrusion blocking column on the reagent box tray stops, after the reagent box is placed and taken out, the instruction is input through the input end of the microcomputer, and the signal processing control unit receives the instruction and controls the moving motor to move towards the discharging direction.
Preferably, the detection device further comprises a software analysis system, the number of each group of white light LED arrays is determined by the number of columns of the kit, each group of white light LED arrays corresponds to one group of photosensitive arrays, the optical collimation component is a metal component, a plurality of cylindrical light guide holes are formed in the interior of each group of white light LED arrays in a digging mode, the inner wall of each light guide hole is smooth and beneficial to light reflection, each small hole corresponds to one white light LED, when in detection, LEDs in the white light LED arrays are lightened, the LEDs pass through the optical collimation component and are reflected for multiple times to form light beams with stronger illumination intensity, the light beams irradiate on the detection holes of the kit and penetrate through the medicine sensitive detection holes to reach the photosensitive arrays, the photosensitive arrays convert received transmitted light into periods, the received transmitted light intensities are different, the obtained periods are different, and when the detection data are detected by a final value method, the detection data are compared with a system preset value, and medicine sensitive results are analyzed and judged. When monitoring is carried out in the culture process, the software analysis system stores the detection values in a database according to the time sequence, and draws a microbial growth curve.
Preferably, the magnetic stirring type drug sensitive analyzer is further provided with a matched kit, the matched kit is a perforated plate with a cover and distributed in an array, the kit is divided into drug sensitive stirring holes and drug sensitive detection holes, each drug sensitive stirring hole corresponds to one drug sensitive detection hole, the drug sensitive stirring holes and the drug sensitive detection holes corresponding to the drug sensitive stirring holes are communication holes, stirring rods are contained in the drug sensitive stirring holes, stirring rods are not contained in the drug sensitive detection holes, the angle and the intensity of transmitted light received by the photosensitive array are not influenced during detection, dried antibiotics are coated in each hole except the positive control hole, the types of antibiotics in each row of drug sensitive stirring holes and the drug sensitive detection holes are the same, and the concentration is gradually increased or decreased in a gradient.
(III) beneficial effects
The invention is matched with a microcomputer for use, has simple operation, short drug sensitivity time and high result accuracy. The drug resistance of the microorganism can be judged by a final value method, the growth state of the microorganism can be monitored in the culture process, the stirring function is increased in the culture process, the propagation speed of the drug-resistant microorganism is accelerated, the time of the whole drug-sensitive process is shortened, and the steps of manual operation are reduced. The magnetic stirring type drug sensitive analysis instrument comprises a constant temperature culture device, a stirring device, a movement device and a detection device. The culture device is a constant temperature device, and the temperature is controlled at 35-37 ℃, and the culture device comprises an electric heating plate, a fan, a thermosensitive element and an incubator. The stirring device comprises a stirring motor, a magnet and a stirrer. The magnet is connected to the rotating shaft of the stirring motor through the adapter, and the stirrer is positioned in the drug sensitive stirring hole of the matched kit. The moving device comprises a moving motor, a displacement platform, a photoelectric sensor and a kit tray. The detection device comprises a white light LED array, an optical collimation component and a photosensitive array. The stirring device, the moving device and the detecting device are positioned inside the incubator. In the process of culturing microorganisms, the stirring motor rotates to drive the magnet to rotate, the stirrer rotates under the action of the rotating magnetic field, and medicines, microorganism liquid, air and nutrient components in the stirring holes are mixed. The propagation speed of drug-resistant microorganisms of the medicine is accelerated, and the process of drug sensitivity is shortened. After a period of cultivation, the moving device moves to the detecting device with the kit, and the cultivation and detection are repeated in this way, so that a microorganism growth curve is finally obtained.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a first partial structure of a magnetic stirring type drug sensitive analyzer;
FIG. 2 is a schematic diagram showing a second partial structure of a magnetic stirring type drug sensitive analyzer;
FIG. 3 is a schematic diagram of a third partial structure of a magnetic stirring type drug sensitive analyzer;
FIG. 4 is a detection flow chart of example 1;
FIG. 5 is a detection flow chart of example 2;
FIG. 6 is a schematic diagram of a partial structure of a kit;
fig. 1-3 reference numerals:
101. the device comprises a base plate, 102, an electric heating plate, 103, a fan, 104, a thermosensitive element, 105, a stirring motor, 106, a magnet, 107, a motion motor, 108, a displacement platform, 109, a first photoelectric sensor, 110, a second photoelectric sensor, 111, a third photoelectric sensor, 112, a fourth photoelectric sensor, 113, a kit tray, 114, a white light LED array, 115, an optical collimating component, 116, a photosensitive array, 117, a first tooth-shaped protrusion, 118, a second tooth-shaped protrusion, 119, a third tooth-shaped protrusion, 120, a fourth tooth-shaped protrusion, 121, a fifth tooth-shaped protrusion, 121, a sixth tooth-shaped protrusion, 122, a seventh tooth-shaped protrusion, 123 and an eighth tooth-shaped protrusion.
Fig. 6 reference numerals:
601. drug sensitive stirring hole 602, drug sensitive detection hole 603, stirrer.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Fig. 1-3 show a schematic partial structure of a magnetic stirring type drug sensitive analyzer, wherein fig. 1 is a schematic partial structure including a part of a culture device and a stirring device, fig. 2 is a schematic partial structure of an increasing motion device of fig. 1, and fig. 3 is a schematic partial structure of an increasing detection device of fig. 2. The electric heating plate 102 and the fan 103 are fixed on the bottom plate 101 through a fixed bracket and are in the same straight line. The magnet 106 is connected to the rotation shaft of the stirring motor 105 through an adapter. The motion motor 107 and the displacement platform 108 are arranged on the bottom plate and are positioned on the same straight line. The motion motor 107 is fixedly connected with a coupling through a screw, and the other end of the coupling is fixedly connected to a rotating shaft of the displacement platform 108 through the screw. One end, close to the motor, of the rotating shaft of the displacement platform 108 is fixedly connected with a kit tray 113 through a conversion piece and a screw. The first, second, third and fourth photosensors 109, 110, 111, 112 are fixed to both sides of the metal plate by fixing brackets, respectively.
Example 1:
example 1 gives a final value method test example. FIG. 6 shows a schematic partial structure of the kit. The final value method drug sensitivity detection is carried out according to the following steps.
Step one, preparing a specimen liquid and inoculating a kit. Preparing specimen liquid with a certain concentration, and adding the prepared specimen liquid into all holes.
And step two, culturing. And placing the prepared specimen kit into an incubator for culturing for 18-24 hours.
And thirdly, detecting on-machine. Fig. 4 shows a detection flow chart of example 1. Step S11, starting operation, entering step S12, wherein a user selects a final value method detection mode through the input end of the microcomputer, the signal processing control unit receives an instruction from the microcomputer, sends out a control signal, and the stirring motor 105 and the moving motor 107 are in a static state and wait for the next instruction. If a user needs to check the specimen kit on the machine, the operation of placing and taking the specimen is needed. Step S13 is performed to judge whether the user inputs a sample placement instruction through the microcomputer terminal. If the user inputs the instruction, the step S14 is entered, and the signal processing control unit sends a control signal to control the movement motor 107 to rotate, so as to push the reagent box tray 113 to move towards the unloading direction, otherwise, the waiting is continued. The first toothed protrusion 117 will block the second photosensor 110 if the discharge position is reached. Step S15 is entered, whether the level of the second photoelectric sensor 110 is changed is judged, if the level is changed, step S16 is entered, the signal processing control unit controls the motion motor 107 to stop moving, and the user waits for the completion of the placement of the specimen reagent kit. If the user takes out the specimen, the process is ended, if the user puts in the specimen, the process proceeds to step S17, and whether the user inputs a warehouse entry instruction through the input end of the microcomputer is judged. If the user inputs a binning instruction, the signal processing control unit controls the movement motor 107 to rotate, the reagent cartridge tray 112 moves in the binning direction, and if the detection position is reached, the third tooth-shaped protrusion 119 blocks the first photoelectric sensor 109. Step S19 is performed to determine whether the level of the first photosensor 109 is changed, and if so, step S110 is performed to control the motion motor 107 to stop at the detection position by the signal processing control unit, where one row of holes at the triangular notch in fig. 6 is the first row of holes. The first row of drug sensitive detection apertures 602 is located directly below the optical collimating component 115. Step S111 is performed, where the signal control processing unit sends out a control signal to control the white LED array 114 to be turned on, so as to enable the photosensitive array 116 to detect the first row of drug sensitive detection holes 602. And storing the corresponding detection hole data into a database, and sending a completion instruction after detection is completed. Step S112 is entered, and the signal processing control unit controls the movement motor 107 to send the second row of drug sensitive detection holes 602 to the detection position. The second row starts to be detected. And (3) detecting the completion of the second row, sequentially detecting a third row and a fourth row … …, proceeding to step S113, judging whether all holes are detected completely, proceeding to step S114 if the detection is completed, comparing the detection data with a system preset value, and analyzing and judging the drug sensitivity result. For example, for a certain bacteria, the system presets a negative-positive demarcation value X1, and the detected data is positive above X1 and negative below X1. The system presets a reference concentration X2. The positive control wells were negative, suggesting error. The positive control hole is positive, and the next judgment is carried out. MIC is less than or equal to X2/5, and is judged to be sensitive (S), MIC is more than X2, and is judged to be drug resistant (R), and X2 is more than or equal to MIC is more than or equal to X2/5, and is judged to be intermediate (I).
Example 2:
example 2 gives examples of monitoring during cultivation. First, a specimen liquid was prepared, and a kit was inoculated, and the procedure was the same as in example 1. Secondly, culturing and detecting on the machine. Fig. 5 shows a detection flow chart of example 2. Step S21, operation is started. Step S22 is entered, and the user selects the monitoring mode via the microcomputer input. The user inputs the sample to be sampled through the microcomputer input terminal, and the process proceeds to step S23, and the sample is sampled in the same manner as in the sample sampling operation of example 1. The sample reagent box is put and taken, the process goes to step S24, the signal processing control unit controls the motion motor 107 to stop at the culture position, and the stirring motor 105 is positioned under the first row of drug sensitive stirring holes. If the user takes out the specimen kit, the process proceeds to step S215. If the user inserts the specimen kit, the step S25 is entered, and the signal processing control unit controls the stirring motor 105 to rotate, the magnet 106 fixed on the rotation shaft of the stirring motor 105 rotates to form a rotating magnetic field, and the stirring rod 603 in the drug sensitive stirring hole 601 is driven to rotate, so that the stirring purpose is achieved. Stirring for a period of time, for example, 1 minute, proceeds to step S26, and judges whether the stirring time reaches 1 minute, if 1 minute is reached, proceeds to step S27, and the signal processing control unit controls the movement motor 107 to move, sends the second row of drug sensitive stirring holes to the upper side of the stirring motor, and starts stirring the second row. The second row stirring is completed and the third and fourth rows … … are stirred sequentially. Step S28 is performed to determine whether all the holes are completely stirred. If stirring is complete. Step S29 is performed, and the stirring is reversed. The stirring was repeated in the forward and reverse directions. The signal processing control unit receives the detection signal, and proceeds to step S210, and returns to the detection position for detection. The specific detection procedure was the same as in example 1. Step S211 is entered, whether all the well detection is completed is judged, the detection is completed, step S212 is entered, and the culture detection step is repeated. And (3) detecting a plurality of times, entering a step S213, wherein the software analysis system fits the data of the plurality of times into a curve, each time the detection is added, the fitted curve is added with one point, and a K value is calculated, wherein K=delta D/delta T, delta D represents the data difference of the two detections, and delta T represents the time difference of the two detections. Step S214 is entered, the detection data are compared with the preset value of the system, and the drug sensitivity result is analyzed and judged. For example, for a certain bacterium, the system presets the culture time T, the system presets the yin-yang boundary K value, the culture time T is reached, the calculated K value is positive above the system preset yin-yang boundary K value, and negative below the system preset yin-yang boundary K value. The system presets a reference concentration X2. The positive control wells were negative, suggesting error. The positive control hole is positive, and the next judgment is carried out. The judgment was the same as in example 1.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element. (columns, rows in the text are also convenient illustrations)
The above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (7)
1. A magnetic stirring type drug sensitive analytical instrument, which is characterized in that: comprises a constant temperature culture device, a stirring device, a motion device and a detection device; the constant temperature culture device comprises an electric heating plate, a fan, a thermosensitive element and an incubator, wherein the thermosensitive element is used for detecting the temperature of air in the incubator and converting the temperature value into a corresponding voltage value, the stirring device comprises a stirring motor, a magnet and a stirrer, the magnet is connected to a rotating shaft of the stirring motor through an adapter, the stirrer is positioned in a drug-sensitive stirring hole of a matched kit, the moving device comprises a moving motor, a displacement platform, a first photoelectric sensor, a second photoelectric sensor, a third photoelectric sensor, a fourth photoelectric sensor and a kit tray, the moving motor and the displacement platform are arranged on the metal plate and are positioned on the same straight line, a shaft coupling is fixedly connected to the motor shaft through screws, the other end of the shaft coupling is fixedly connected to the rotating shaft of the displacement platform through screws, and the detecting device comprises a white light LED array, an optical collimating component and a photosensitive array;
the first photoelectric sensor, the second photoelectric sensor, the third photoelectric sensor and the fourth photoelectric sensor are opposite-type photoelectric sensors, the first photoelectric sensor is blocked by an opaque object, signals at the output end change, the first photoelectric sensor is fixed on a first fixed support on one side of a metal plate, the second photoelectric sensor, the third photoelectric sensor and the fourth photoelectric sensor are respectively fixed on a second fixed support, a third fixed support and a fourth fixed support on the other side of the metal plate, during culture, the fourth photoelectric sensor is blocked by a second tooth-shaped protrusion, the electric level changes, the stirring device is used for stirring substances in a first row of medicine-sensitive stirring holes, after a period of time, a motion motor is rotated for a certain step number in sequence, the stirring device is used for stirring the substances in the second row of medicine-sensitive stirring holes, the third row and the fourth row of medicine-sensitive stirring holes are sequentially stirred downwards, when the electric level changes, all the substances in the medicine-sensitive stirring holes can be stirred, and simultaneously the motion motor is moved reversely, and the first row and the second row of medicine-sensitive stirring holes are sequentially stirred downwards by hardware;
after the microorganism is cultured for a period of time, the moving device moves to a detection position with the kit, the third tooth-shaped protrusion blocks the first photoelectric sensor, the first row is detected, the moving motor rotates, the fourth tooth-shaped protrusion blocks the first photoelectric sensor, the second row is detected, the third row and the fourth row are sequentially detected to be downwards, the repeated culture and detection are carried out, when the kit is placed and taken out, the input end of the microcomputer inputs a discharging instruction, the signal processing control unit receives the instruction and then controls the moving motor to push the kit tray to move towards the discharging direction, the first tooth-shaped protrusion blocking column on the kit tray stops, after the kit is placed and taken out, the input end of the microcomputer inputs a feeding instruction, and the signal processing control unit receives the instruction and controls the moving motor to move towards the feeding direction;
the detection device further comprises a software analysis system, the number of each group of white light LED arrays is determined by the number of columns of the kit, each group of white light LED arrays corresponds to one group of photosensitive arrays, the optical collimation component is a metal component, a plurality of cylindrical light guide holes are formed in the interior of each group of white light LED arrays in a matting way, the inner wall of each light guide hole is smooth and beneficial to light reflection, each small hole corresponds to one white light LED, when in detection, LEDs in the white light LED arrays are lightened, the LEDs pass through the optical collimation component and form light beams with stronger illumination intensity through the optical collimation component, the light beams irradiate on the medicine sensitive detection holes of the kit and penetrate the medicine sensitive detection holes to reach the photosensitive arrays, the photosensitive arrays convert received transmitted light into periods, the received transmitted light intensities are different, the obtained periods are different, when the detection data are detected by a final value method, the detection data are compared with preset values of the system, the medicine sensitive results are analyzed and judged, when the detection values are monitored in the cultivation process, the detection values are stored in a database according to the time sequence, and the detection values are drawn into a microbial growth curve.
2. The magnetically stirred drug sensitive analytical instrument according to claim 1, wherein: the insulation can is divided into a bottom plate and a box body, the bottom plate is composed of three layers of different materials of a metal plate, insulation cotton and a plastic plate, the three layers of different materials are overlapped and placed, the insulation cotton is located between the metal plate and the plastic plate, the box body is composed of the insulation cotton and the plastic plate, the metal plate is high in strength, the insulation can is supported by the inner structure, the insulation cotton reduces heat loss in the insulation can, a fan hole is reserved at the rear side of the box body, an electric heating plate and a fan are fixed on the metal plate through a fixing support and are located on the same straight line, the fan faces the fan hole at the rear side of the box body of the insulation can, and the electric heating plate, the fan and a thermosensitive element are connected to a signal control processing unit through respective control circuits.
3. The magnetically stirred drug sensitive analytical instrument according to claim 2, wherein: the temperature value is preset in the internal register of the signal control processing unit, the preset temperature value is a temperature range, the fan is in a continuous working state, the electric heating plate is in an intermittent working state, the temperature detected by the thermosensitive element is higher than the preset temperature value, the electric heating plate does not work, the fan brings cold air outside the insulation box into the box body, the temperature is accelerated to be reduced, the detected temperature is lower than the preset temperature value, the electric heating plate works, the fan brings heat of the heating plate into the box body, the air inside the insulation box is stirred, and the cold air and the hot air are uniformly mixed.
4. The magnetically stirred drug sensitive analytical instrument according to claim 1, wherein: the stirring motor rotates to drive the magnet to rotate, the stirrer positioned right above the magnet rotates under the action of a rotating magnetic field, and medicines, microorganism liquid and nutrient component air in the medicine-sensitive stirring holes are fully mixed to accelerate the propagation speed of medicine-resistant microorganisms of the medicines and shorten the medicine-sensitive process.
5. The magnetically stirred drug sensitive analytical instrument according to claim 1, wherein: the utility model discloses a quick detection hole detection device, including displacement platform, reagent box tray, reagent box, displacement platform pivot, changeover piece, screw fixed connection reagent box tray, set up a plurality of reagent boxes on the reagent box tray and place the district, the size that the reagent box was placed the district is confirmed according to the reagent box size, leave a plurality of round holes on the reagent box is placed the district panel, be array form distribution, the quantity, the size of round hole and the distance between the aperture are unanimous with quick detection hole in the reagent box.
6. The magnetically stirred tank type drug sensitive analytical instrument according to claim 5, wherein: a row of dentate protrusions are arranged on one side of the kit tray, the number of the dentate protrusions is consistent with the number of the drug sensitive detection holes in the kit, the distance between the dentate protrusions is consistent with the distance between the centers of the drug sensitive detection holes in the adjacent rows in the kit, 2 dentate protrusions are respectively arranged on the other side of the kit tray, the dentate protrusions are a first dentate protrusion and a second dentate protrusion, and the distance between the first dentate protrusion and the second dentate protrusion is consistent with the distance between the drug sensitive stirring holes in the first row and the drug sensitive stirring holes in the last row in the kit.
7. The magnetically stirred drug sensitive analytical instrument according to claim 1, wherein: the magnetic stirring type drug sensitive analyzer is characterized in that the magnetic stirring type drug sensitive analyzer is further provided with a matched kit, the matched kit is a perforated plate with a cover and distributed in an array shape, the kit is divided into drug sensitive stirring holes and drug sensitive detection holes, each drug sensitive stirring hole corresponds to one drug sensitive detection hole, the drug sensitive stirring holes and the drug sensitive detection holes corresponding to the drug sensitive stirring holes are communication holes, stirring rods are contained in the drug sensitive stirring holes, stirring rods are not contained in the drug sensitive detection holes, the angle and the intensity of transmitted light received by a photosensitive array are not influenced during detection, each hole except a positive control hole is further coated with dried antibiotics, the types of antibiotics in each row of drug sensitive stirring holes and the drug sensitive detection holes are the same, and the concentration of the antibiotics in each row of drug sensitive stirring holes and the drug sensitive detection holes is gradually increased or decreased in a gradient.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710254526.6A CN106916728B (en) | 2017-04-18 | 2017-04-18 | Magnetic stirring type drug sensitive analytical instrument and matched kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710254526.6A CN106916728B (en) | 2017-04-18 | 2017-04-18 | Magnetic stirring type drug sensitive analytical instrument and matched kit |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106916728A CN106916728A (en) | 2017-07-04 |
| CN106916728B true CN106916728B (en) | 2023-10-27 |
Family
ID=59567432
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710254526.6A Active CN106916728B (en) | 2017-04-18 | 2017-04-18 | Magnetic stirring type drug sensitive analytical instrument and matched kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106916728B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107991483A (en) * | 2017-12-28 | 2018-05-04 | 天津绿禾益民生物质利用科技有限公司 | A kind of intelligent quick detection biotinylation kit |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1137786A (en) * | 1979-10-05 | 1982-12-21 | Richard K. Wertz | Automatic scanning apparatus for performing optical density tests on liquid samples, method for testing for antibiotic susceptibility, and method for identifying microorganisms |
| US4448534A (en) * | 1978-03-30 | 1984-05-15 | American Hospital Corporation | Antibiotic susceptibility testing |
| US6537772B1 (en) * | 1997-04-18 | 2003-03-25 | Centro Nacional De Investigaciones | Equipment, kit and method for microbiological diagnosis |
| CN101555516A (en) * | 2009-05-15 | 2009-10-14 | 钱叶林 | Candida albicans rapid identification and cultivation susceptibility test kit and test method |
| CN101886111A (en) * | 2010-06-11 | 2010-11-17 | 郑州安图绿科生物工程有限公司 | Fungus rapid culture, identification and susceptibility detection kit and detection method thereof |
| CN103740802A (en) * | 2014-01-15 | 2014-04-23 | 珠海市银科医学工程有限公司 | Mycobacterium drug sensitive detection kit and testing method thereof |
| CN105136684A (en) * | 2015-08-14 | 2015-12-09 | 上海蓝怡科技股份有限公司 | Multi-sample detection device and method |
| CN106442985A (en) * | 2016-09-13 | 2017-02-22 | 珠海美华医疗科技有限公司 | Full-automatic quick microbiological identification and drug sensitivity analysis system |
| CN206768082U (en) * | 2017-04-18 | 2017-12-19 | 朱红 | A kind of magnetic stirring Analysis of Drug Susceptibility instrument and matched reagent box |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101796906B1 (en) * | 2009-03-24 | 2017-11-10 | 유니버시티 오브 시카고 | Method for carrying out a reaction |
-
2017
- 2017-04-18 CN CN201710254526.6A patent/CN106916728B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4448534A (en) * | 1978-03-30 | 1984-05-15 | American Hospital Corporation | Antibiotic susceptibility testing |
| CA1137786A (en) * | 1979-10-05 | 1982-12-21 | Richard K. Wertz | Automatic scanning apparatus for performing optical density tests on liquid samples, method for testing for antibiotic susceptibility, and method for identifying microorganisms |
| US6537772B1 (en) * | 1997-04-18 | 2003-03-25 | Centro Nacional De Investigaciones | Equipment, kit and method for microbiological diagnosis |
| CN101555516A (en) * | 2009-05-15 | 2009-10-14 | 钱叶林 | Candida albicans rapid identification and cultivation susceptibility test kit and test method |
| CN101886111A (en) * | 2010-06-11 | 2010-11-17 | 郑州安图绿科生物工程有限公司 | Fungus rapid culture, identification and susceptibility detection kit and detection method thereof |
| CN103740802A (en) * | 2014-01-15 | 2014-04-23 | 珠海市银科医学工程有限公司 | Mycobacterium drug sensitive detection kit and testing method thereof |
| CN105136684A (en) * | 2015-08-14 | 2015-12-09 | 上海蓝怡科技股份有限公司 | Multi-sample detection device and method |
| CN106442985A (en) * | 2016-09-13 | 2017-02-22 | 珠海美华医疗科技有限公司 | Full-automatic quick microbiological identification and drug sensitivity analysis system |
| CN206768082U (en) * | 2017-04-18 | 2017-12-19 | 朱红 | A kind of magnetic stirring Analysis of Drug Susceptibility instrument and matched reagent box |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106916728A (en) | 2017-07-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102116771B (en) | Automatic enzyme-linked immune analysis analyzer | |
| US6271022B1 (en) | Device for incubating and monitoring multiwell assays | |
| US7041493B2 (en) | Bioreactor and bioprocessing technique | |
| WO2013044454A1 (en) | Full-automatic immunity analyzer and detection method thereof | |
| CN2807259Y (en) | Full-automatic biochemical analyzer | |
| CN102131914A (en) | Apparatus and method for bacteriological testing on plasma | |
| CN107828648B (en) | Constant-temperature continuous automatic microbe culture and detection drug sensitivity analysis device | |
| EP0261202A1 (en) | Automated multi-purposse analytical chemistry processing center and laboratory work station | |
| JP2003014769A (en) | Automatic analyzer | |
| JPH07327662A (en) | Small-sized blood culture device | |
| JP2002125697A (en) | System and method for analyzing antibiotic susceptibility of biological sample | |
| CN111289295A (en) | Online sampling detection device and method for biological reaction process | |
| CN109459400A (en) | Protein analyzer and its test method | |
| CA2016872C (en) | Apparatus and method for detection of microorganisms | |
| CN207280957U (en) | Full-automatic intelligent liquid pesticidal residue detector | |
| CN206768082U (en) | A kind of magnetic stirring Analysis of Drug Susceptibility instrument and matched reagent box | |
| CN106916728B (en) | Magnetic stirring type drug sensitive analytical instrument and matched kit | |
| CN110346586A (en) | Full-automatic detection apparatus | |
| JP3666621B2 (en) | Microbe-derived component measuring apparatus and measuring method | |
| CN114019089A (en) | Automatic titration device for supporting chemical oxygen demand in water | |
| CN210376404U (en) | Full-automatic detection device | |
| CN218710489U (en) | Automatic fungus liquid preparation and inoculation workstation of changing | |
| EP0304406A2 (en) | Apparatus for automatically counting the microorganisms possibly present in liquids, particularly in waters for human use | |
| CN111455017A (en) | Method for measuring total number of microbial colonies in food | |
| KR102576448B1 (en) | Automatic inspection system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |