CN203048943U - Kit with fungi identification and fast drug sensitivity test functions - Google Patents
Kit with fungi identification and fast drug sensitivity test functions Download PDFInfo
- Publication number
- CN203048943U CN203048943U CN 201220718377 CN201220718377U CN203048943U CN 203048943 U CN203048943 U CN 203048943U CN 201220718377 CN201220718377 CN 201220718377 CN 201220718377 U CN201220718377 U CN 201220718377U CN 203048943 U CN203048943 U CN 203048943U
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- shrinkage pool
- fungi
- culture plate
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- susceptibility
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Abstract
The utility model discloses a kit with fungi identification and fast drug sensitivity test functions, and the kit comprises a box body, wherein a porous culture plate, a normal saline reagent and a liquid fungi culture medium reagent are placed in the box body; the porous culture plate is provided with a negative detection concave hole, a culture concave hole and at least one drug property comparison concave hole; the drug property comparison concave holes are internally provided with tested drugs; the porous culture plate is provided with a cover plate; and the cover plate is of a transparent structure. The kit disclosed by the utility model greatly shortens the test period by completing pathogenic fungi identification and drug sensitivity test through one step within 1-5 days, is suitable for the clinical early diagnosis and enables the patients to enter a treatment period as early as possible.
Description
Technical field
The utility model belongs to the vitro diagnostic techniques field, is specifically related to the test kit of a kind of tool fungi evaluation and the quick measurement function of susceptibility.
Background technology
What be used in the market that the evaluation of pathomycete cultivates mainly is culture method, usually diagnostic method is, after cultivating about 2～3 days by flat board, observe colonial morphology, be defined as after the pathomycete, picking colony again, then utilize pathomycete to the different characteristics of different antibacterials susceptibility, analyze resistance with diffusion process (paper disk method), concrete steps are as follows: the filter paper that will contain quantitative antibacterials is attached on the solid culture primary surface of having inoculated the test bacterium, medicine in the scraps of paper spreads in substratum, reduce along with the concentration of the increase antibacterials of diffusion length is logarithm, thereby form a kind of concentration gradient around the scraps of paper, the test bacterium around the scraps of paper in the Mlc scope can not grow, the then continued growth of the extraneous bacterial strain of Mlc, thus transparent inhibition zone around the scraps of paper, formed.In general, the big I reflection of inhibition zone is tested bacterium to the sensitivity of mensuration medicine, and with this medicine the minimum inhibitory concentration of testing bacterium is negative correlation, and namely inhibition zone is more big, and minimum inhibitory concentration is more little.Paper disk method can be measured multiple antibacterials simultaneously, and has the characteristics cheap, that environmental compatibility is strong.Above-mentioned culture method and paper disk method are all finished about time of 10 days of needs, can't satisfy the expectation that the patient wants rapid healing.
In addition, the porous culture plate on the existing market arranges in the nontransparent lid usually, when test is finished, need open lid and could observe colonial morphology, and prolific thalline might permeate in the air by this operation, and then influence testing crew health.
The utility model content
The utility model provides a kind of tool fungi to identify and the test kit of the quick measurement function of susceptibility, and its purpose is to solve long, the defective that can not direct viewing of available reagent box test period.
The technical solution of the utility model is as follows:
The test kit of the evaluation of tool fungi and the quick measurement function of susceptibility, comprise box body, placing porous culture plate, physiological saline reagent and liquid fungi culture medium reagent in this box body, described porous culture plate is provided with negative shrinkage pool, cultivation shrinkage pool and the property of medicine of at least one the contrast shrinkage pool of detecting, and in each property of medicine contrast shrinkage pool detection of drugs is installed; Described porous culture plate is provided with a cover plate, and described cover plate is transparent configuration.
In the embodiment that recommends, described negative shrinkage pool, cultivation shrinkage pool and each property of medicine contrast shrinkage pool of detecting has identical aperture and the degree of depth.
In the embodiment that recommends, the detection of drugs of different sorts or various dose is installed in each property of medicine contrast shrinkage pool.
In the embodiment that recommends, labeling section is set above or below each shrinkage pool of described porous culture plate, to record reagent name, detection of drugs title or mentioned reagent and the corresponding numbering of putting in the corresponding shrinkage pool of detection of drugs.
In the embodiment that recommends, described porous culture plate is transparent configuration equally.
In the embodiment that recommends, described detection of drugs is selected from least a in KETOKONAZOL, miconazole, itraconazole, fluconazole, econazole, clotrimazole, undecylenic acid, acetic acid, lactic acid, Whitfield's ointment, grisovin, nystatin, kenianjunsu, flucytosine, amphotericin B, globoroseomycin or the methylpartricin.
In the embodiment that recommends, described liquid fungi culture medium reagent is potato dextrose agar, Ma Dingshi substratum or broth culture.
Compared with prior art to description of the present utility model as can be known by above-mentioned, advantage of the present utility model is:
1, the utility model can be in 1-5 days a step finish pathomycete and identify and medicament sensitivity test, shorten the test period greatly, be suitable for clinical early diagnosis, make the patient enter treatment cycle as early as possible;
2, the cover plate of the utility model porous culture plate is made by transparent material, makes the testing crew can direct viewing colony growth form, great convenience is provided not only for user's observation work, but and the protection test personnel avoid the infringement of fungi.
Description of drawings
Fig. 1 is the structural representation of test kit of the present utility model.
Embodiment
Embodiment of the present utility model is described with reference to the accompanying drawings.
The test kit of the evaluation of tool fungi and the quick measurement function of susceptibility, shown in Fig. 1, it comprises box body 1, places physiological saline reagent 2, Ma Dingshi substratum reagent 3 and porous culture plate 5 in this box body 1.
Porous culture plate 5 is provided with negative shrinkage pool 51, cultivation shrinkage pool 52 and several the property of medicine contrast shrinkage pool 53 of detecting.This wherein, the negative shrinkage pool 51 that detects is arranged to independent delegation jointly with cultivation shrinkage pool 52,53 branches of several property of medicine contrast shrinkage pools are set as two row, the property of medicine contrast shrinkage pool 53 of previous row is placed with the medicine of low dosage, then is placed with same a kind of medicine of high dosage in the corresponding property of medicine contrast shrinkage pool 53 of back delegation.Negative shrinkage pool 51, cultivation shrinkage pool 52 and each property of medicine contrast shrinkage pool 53 of detecting has identical aperture and the degree of depth.
Porous culture plate 5 is provided with a cover plate (not shown), and this cover plate is transparent configuration.
Porous culture plate 5 is transparent configuration equally, and the below of its each shrinkage pool arranges labeling section 54, to record reagent name, detection of drugs title or mentioned reagent and the corresponding numbering of putting in the corresponding shrinkage pool of detection of drugs.
In each property of medicine contrast shrinkage pool 53 of first row different types of detection of drugs is installed, detection of drugs is selected from least a in KETOKONAZOL, miconazole, itraconazole, fluconazole, econazole, clotrimazole, undecylenic acid, acetic acid, lactic acid, Whitfield's ointment, grisovin, nystatin, kenianjunsu, flucytosine, amphotericin B, globoroseomycin or the methylpartricin.
Detection of drugs kind in the corresponding property of medicine contrast with first row of the detection of drugs shrinkage pool of installing in each property of medicine contrast shrinkage pool 53 of second row 53 is identical, and dosage then is three times in the detection of drugs of each property of medicine contrast shrinkage pool 53 of first row.
The method of utilizing the utility model test kit to detect, following steps specifically are installed implement:
Step 1: get Ma Dingshi substratum 100 μ l from Ma Dingshi substratum reagent 3, the feminine gender that joins on the porous culture plate detects in the shrinkage pool 51;
Step 2: get a physiological saline reagent 2, adsorb physiological saline with cotton swab, at the twisted several of diseased region, take out cotton swab, place immediately in remaining Ma Dingshi substratum, stir cotton swab for several times, extract cotton swab, squeeze out wherein liquid facing to the bottle wall as far as possible, take out cotton swab, mixing obtains having the Ma Dingshi substratum of fungi;
Step 3: get the Ma Dingshi substratum 100 μ l that have fungi, join respectively in the cultivation shrinkage pool 52 and each property of medicine contrast shrinkage pool 53 on the porous culture plate;
Step 4: cover the plate lid, place the porous culture plate 37 ℃ of incubators to cultivate;
Observe in step 6:1～5 day and read the result, interpretation standard is as follows:
(1) if the increment of negative detection shrinkage pool 51 fungies then shows pollution, invalidate the test;
(2) if negative detection shrinkage pool 51 fungies do not have increment, cultivate shrinkage pool 52 increments, sampling judges whether to be pathomycete, if be judged as pathomycete, and remaining each property of medicine contrast shrinkage pool 53 result of judgement;
A: if the flora growing state of property of medicine contrast shrinkage pool 53 is suitable with the flora growing state of cultivating shrinkage pool 52, represent insensitive to this medicine;
B: if the flora growing state of property of medicine contrast shrinkage pool 53 is poorer than the flora growing state of cultivating shrinkage pool 52, expression is to this medicaments insensitive.
Then, again according to the flora growing state of low dosage detection of drugs shrinkage pool and high dosage detection of drugs shrinkage pool, preliminary judgement is formulated dosage to the patient.
Above-mentioned only is embodiment of the present utility model, but design concept of the present utility model is not limited thereto, and allly utilizes this design that the utility model is carried out the change of unsubstantiality, all should belong to the behavior of invading the utility model protection domain.
Claims (5)
1. the test kit of the fungi of having evaluation and the quick measurement function of susceptibility, it is characterized in that: comprise box body, placing porous culture plate, physiological saline reagent and liquid fungi culture medium reagent in this box body, described porous culture plate is provided with negative shrinkage pool, cultivation shrinkage pool and the property of medicine of at least one the contrast shrinkage pool of detecting, and in each property of medicine contrast shrinkage pool detection of drugs is installed; Described porous culture plate is provided with a cover plate, and described cover plate is transparent configuration.
2. according to the test kit of the evaluation of the tool fungi described in the claim 1 and the quick measurement function of susceptibility, it is characterized in that: described negative shrinkage pool, cultivation shrinkage pool and each property of medicine contrast shrinkage pool of detecting has identical aperture and the degree of depth.
3. according to the test kit of the evaluation of the tool fungi described in the claim 2 and the quick measurement function of susceptibility, it is characterized in that: the detection of drugs that different sorts or various dose are installed in each property of medicine contrast shrinkage pool.
4. according to the test kit of the evaluation of the tool fungi described in claim 1 or 2 or 3 and the quick measurement function of susceptibility, it is characterized in that: above or below each shrinkage pool of described porous culture plate labeling section is set.
5. according to the test kit of the evaluation of the tool fungi described in claim 1 or 2 or 3 and the quick measurement function of susceptibility, it is characterized in that: described porous culture plate is transparent configuration equally.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220718377 CN203048943U (en) | 2012-12-24 | 2012-12-24 | Kit with fungi identification and fast drug sensitivity test functions |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220718377 CN203048943U (en) | 2012-12-24 | 2012-12-24 | Kit with fungi identification and fast drug sensitivity test functions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203048943U true CN203048943U (en) | 2013-07-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220718377 Expired - Fee Related CN203048943U (en) | 2012-12-24 | 2012-12-24 | Kit with fungi identification and fast drug sensitivity test functions |
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| Country | Link |
|---|---|
| CN (1) | CN203048943U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047981A (en) * | 2016-08-15 | 2016-10-26 | 郑州点石生物技术有限公司 | Kit for fungal rapid culture, identification and drug sensitivity |
-
2012
- 2012-12-24 CN CN 201220718377 patent/CN203048943U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047981A (en) * | 2016-08-15 | 2016-10-26 | 郑州点石生物技术有限公司 | Kit for fungal rapid culture, identification and drug sensitivity |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130710 Termination date: 20131224 |