CN202543209U - Quick identifying culturing drug sensitivity kit for streptococcus mutans - Google Patents
Quick identifying culturing drug sensitivity kit for streptococcus mutans Download PDFInfo
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- CN202543209U CN202543209U CN 201220108791 CN201220108791U CN202543209U CN 202543209 U CN202543209 U CN 202543209U CN 201220108791 CN201220108791 CN 201220108791 CN 201220108791 U CN201220108791 U CN 201220108791U CN 202543209 U CN202543209 U CN 202543209U
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- streptococcus mutans
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Abstract
The utility model relates to a streptococcus mutans identification technology and discloses a quick identifying culturing drug sensitivity kit for streptococcus mutans. The quick identifying culturing drug sensitivity kit comprises a kit and a drug sensitivity plate and is characterized in that the kit comprises a kit body, an ultra-pure water reagent bottle, a paraffinic oil reagent bottle, a normal saline reagent bottle, and a culture dish contained with a streptococcus mutans quick identifying culture medium, wherein the ultra-pure water reagent bottle, the paraffinic oil reagent bottle and the normal saline reagent bottle are arranged in the kit body; the drug sensitivity plate comprises a plate body and a plate cover; a negative detecting hole and a positive detecting hole are arranged on the plate body; two rows of drug property contrast holes are correspondingly arranged in the negative detecting hole and the positive detecting hole; and detecting drugs are respectively contained in one row of drug property contrast holes corresponding to the positive detecting hole. The quick identifying culturing drug sensitivity kit for the streptococcus mutan is simple to operate and short in detecting period; and with the adoption of the quick identifying culturing drug sensitivity kit, the problems that time and labor are wasted in current streptococcus mutan identifying culturing method.
Description
Technical field
The utility model relates to the Streptococcus mutans authenticate technology, is specifically related to a kind of Streptococcus mutans Rapid identification and cultivates the susceptibility test kit.
Background technology
Streptococcus mutans is a kind of endogenous bacterium, and the respiratory tract, plaque etc. that can be present in healthy subjects are located, and belong to conditioned pathogen.Children's and the elderly's carious tooth are a kind of common odontopathy, are wherein caused by Streptococcus mutans greatly.
At present, be used for the main employing culture method of the evaluation cultivation of Streptococcus mutans on the market, the drug sensitive test product does not then have.Common evaluation cultural method is, cultivate about 2～3 days through flat board after, observe colonial morphology, confirm as after the Streptococcus mutans, picking colony is analyzed resistance with paper disk method again, front and back are accomplished and are identified and approximately need 7 days time.Operator are had higher requirements, and waste time and energy, be not suitable for the requirement of clinical Rapid identification.
Summary of the invention
The purpose of the utility model provides a kind of Streptococcus mutans Rapid identification and cultivates the susceptibility test kit, has solved existing Streptococcus mutans and has identified the problem that wastes time and energy that exists in the cultural method.
In order to achieve the above object, the utility model adopts following technical scheme to be achieved.
A kind of Streptococcus mutans Rapid identification is cultivated the susceptibility test kit; Comprise test kit part and drug sensitive plate part; It is characterized in that; Said test kit partly comprises box body, and the petridish that places the intravital ultrapure water reagent bottle of box, Yellow Protopet 2A reagent bottle, saline water reagent bottle, interior dress Streptococcus mutans Rapid identification substratum; Said drug sensitive plate partly comprises plate body and Ban Gai; Said plate body is provided with negative hole, the positive detection hole detected; Detecting hole and positive detection hole corresponding to feminine gender is provided with the corresponding delegation's property of medicine in two row property of medicine contrast holes and positive detection hole and contrasts the hole and detection of drugs is housed in respectively.
The characteristics of the utility model are, the property of medicine contrast hole of in corresponding delegation property of medicine contrast hole, said positive detection hole is respectively penicillium mould, Oxacyclotetradecane,erythromycin deriv, Azythromycin, tsiklomitsin, levofloxacin, trimethoprim-sulfamethoxazole, clindamycin, vancomyein, Roxithromycin, Spiramycin Base, ceftriaxone or sulperazone being housed.
Utilize the utility model Streptococcus mutans Rapid identification to cultivate the susceptibility test kit and can carry out Rapid identification; Its concrete steps are implemented as follows: step 1; Get the petridish of ultrapure water reagent bottle and interior dress Streptococcus mutans Rapid identification substratum; Join ultrapure water in the Streptococcus mutans Rapid identification substratum, after the dissolving, obtain nutrient solution fully; Step 2 is got nutrient solution 50～100 μ l that step 1 obtains, and the feminine gender that joins on the drug sensitive plate detects in the hole; Step 3 is got the saline water reagent bottle, adsorbs saline water with cotton swab; At the twisted several of diseased region, take out cotton swab, place immediately in the nutrient solution that step 1 obtains; Stir cotton swab for several times, extract cotton swab, and squeeze out wherein liquid as far as possible; Take out cotton swab, shake mixing for several times or with oscillator, obtain mixed nutrient solution with sample injector piping and druming; Step 4 is got mixed nutrient solution 50～100 μ l that step 3 obtains, and joins respectively in the positive detection hole and property of medicine contrast hole on the drug sensitive plate; Step is got the Yellow Protopet 2A reagent bottle, drips a dropstone wax oil (50 μ l) in each hole on drug sensitive plate, covers plate lid 10, places drug sensitive plate 35～37 ℃ of incubators to cultivate; Step 6 is observed in 6～24 hours and read the result, and interpretation standard is following: (1) if feminine gender detects the hole nondiscoloration, the nondiscoloration of positive detection hole then shows no Streptococcus mutans growth, and remaining hole does not make a decision; (2) if negative detection hole variable color then shows pollution, invalidate the test; (3) if feminine gender detects the hole nondiscoloration, the variable color of positive detection hole, showing has Streptococcus mutans in the sample, judge property of medicine contrast each hole result of hole at last.
The utility model utilizes test kit and drug sensitive plate to combine, and a step accomplishes and identifies, has broken through the time limitation of culture-based method, in 6～24 hours, can accomplish the cultivation of Streptococcus mutans; Also added the drug sensitive test of Streptococcus mutans, when Streptococcus mutans is identified cultivation, also accomplished drug resistance analysis, filled up the blank that does not have special Streptococcus mutans susceptibility analysed preparation in the industry the Streptococcus mutans clinical strain.In addition, this product drug sensitive plate encapsulate concentration, also can have certain significance for reference to clinical application, can avoid antibiotic abuse to a certain extent.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the utility model is elaborated.
Fig. 1 is the structural representation of the utility model test kit part;
Fig. 2 is the structural representation of the utility model drug sensitive plate part plate body;
Fig. 3 is the structural representation of the utility model drug sensitive plate part plate lid.
Among the figure, 1, box body; 2, ultrapure water reagent bottle; 3, Yellow Protopet 2A reagent bottle; 4, saline water reagent bottle; 5, petridish; 6, the negative hole of detecting; 7, positive detection hole; 8, property of medicine contrast hole; 9, plate body; 10, Ban Gai.
Embodiment
The Streptococcus mutans Rapid identification of the utility model is cultivated the susceptibility test kit, comprises test kit part and drug sensitive plate part.
With reference to Fig. 1, test kit partly comprises box body 1, is placed with the petridish 5 of dress Streptococcus mutans Rapid identification substratum in two ultrapure water reagent bottles 2, Yellow Protopet 2A reagent bottle 3, saline water reagent bottle 4 and in the box body 1.
With reference to Fig. 2 and Fig. 3, be the plate body 9 and the plate lid 10 of the part of drug sensitive plate.Plate body 9 is provided with negative hole 6 and the positive detection hole 7 detected; Detect hole 6 corresponding to feminine gender and be provided with two row property of medicine contrast holes 8 with positive detection hole 7; Be A capable property of medicine contrast hole and the capable property of medicine contrast of B hole (being encoded to B1-B14), the capable property of medicine contrast of B is equipped with detection of drugs in the hole.In the present embodiment, the capable property of medicine contrast of B hole has utilized totally 13 of B1-B12, and other are subsequent use; Wherein the built-in detection of drugs of B1-B11 is as shown in table 1.
Table 1
Utilize the utility model Streptococcus mutans Rapid identification to cultivate the susceptibility test kit and can carry out Rapid identification, its concrete steps are implemented as follows:
(1) if feminine gender detects hole 6 nondiscolorations, 7 nondiscolorations of positive detection hole then show no Streptococcus mutans growth, and remaining hole does not make a decision;
(2) if negative detection hole 6 variable colors then show pollution, invalidate the test;
(3) if feminine gender detects hole 6 nondiscolorations, 7 variable colors of positive detection hole, showing has Streptococcus mutans in the sample, judge remaining each hole result;
The capable corresponding property of medicine contrast of a:A hole 8 nondiscolorations, the capable corresponding property of medicine contrast of B hole 8 nondiscolorations, the medicaments insensitive that expression is corresponding to these row;
The capable corresponding property of medicine contrast of b:A hole 8 nondiscolorations, the capable corresponding property of medicine contrast of B hole 8 variable colors, the medium sensitivity of medicine that expression is corresponding to these row;
The capable corresponding property of medicine contrast of c:A hole 8 variable colors, the capable corresponding property of medicine contrast of B hole 8 variable colors, expression is insensitive to the corresponding medicine of these row.
Embodiment
Get the petridish 5 of ultrapure water reagent bottle 2 and interior dress Streptococcus mutans Rapid identification substratum, join the 1.5ml ultrapure water in the petridish 5, after the dissolving, obtain nutrient solution fully; Get the nutrient solution 50 μ l that the step obtains, the feminine gender that joins on the drug sensitive plate detects in the hole 6; Get saline water reagent bottle 4, adsorb saline water, at the twisted several of diseased region with cotton swab; Take out cotton swab, place immediately to go on foot in the nutrient solution that obtains, stir cotton swab for several times; Extract cotton swab, squeeze out wherein liquid as far as possible, take out cotton swab facing to the bottle wall; Shake mixing for several times or with oscillator with sample injector piping and druming, obtain mixed nutrient solution; Get the mixed nutrient solution 50 μ l that the step obtains, the positive detection hole 7 that joins respectively on the drug sensitive plate contrasts in the hole 8 with the property of medicine; Get Yellow Protopet 2A reagent 3, Dropwise 50 μ l Yellow Protopet 2A in each hole on drug sensitive plate covers plate lid 10, places drug sensitive plate 35～37 ℃ of incubators to cultivate; Take out after 6 hours, identify according to following rule then.
Negative control hole is red, and the positive control hole is yellow, and showing has Streptococcus mutans in the sample, judges remaining each hole result:
The capable property of medicine contrast of penicillin A hole is red, and the capable property of medicine contrast of B hole is red, shows that this Streptococcus mutans is to the penicillium mould medicaments insensitive; The capable property of medicine contrast of A hole is red, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is to the medium sensitivity of penicillium mould medicine; The capable property of medicine contrast of A hole is yellow, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is insensitive to the penicillium mould medicine.
The capable property of medicine contrast of Erythromycin A hole is red, and the capable property of medicine contrast of B hole is red, shows that this Streptococcus mutans is to the Oxacyclotetradecane,erythromycin deriv medicaments insensitive; The capable property of medicine contrast of A hole is red, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is to the medium sensitivity of Oxacyclotetradecane,erythromycin deriv medicine; The capable property of medicine contrast of A hole is yellow, and the capable property of medicine contrast of B hole shows that this Streptococcus mutans is insensitive to the Oxacyclotetradecane,erythromycin deriv medicine.
The capable property of medicine contrast of Azythromycin A hole is red, and the capable property of medicine contrast of B hole is red, shows that this Streptococcus mutans is to the Azythromycin medicaments insensitive; The capable property of medicine contrast of A hole is red, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is to the medium sensitivity of Azythromycin medicine; The capable property of medicine contrast of A hole is yellow, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is insensitive to the Azythromycin medicine.
The capable property of medicine contrast of tsiklomitsin A hole is red, and the capable property of medicine contrast of B hole is red, shows that this Streptococcus mutans is to the tsiklomitsin medicaments insensitive; The capable property of medicine contrast of A hole is red, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is to the medium sensitivity of tsiklomitsin medicine; The capable property of medicine contrast of A hole is yellow, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is insensitive to the tsiklomitsin medicine;
The capable property of medicine contrast of levofloxacin A hole is red, and the capable property of medicine contrast of B hole is red, shows that this Streptococcus mutans is to the levofloxacin medicaments insensitive; The capable property of medicine contrast of A hole is red, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is to the medium sensitivity of levofloxacin medicine; The capable property of medicine contrast of A hole is yellow, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is insensitive to the levofloxacin medicine;
The capable property of medicine contrast of trimethoprim-sulfamethoxazole A hole is red, and the capable property of medicine contrast of B hole is red, shows that this Streptococcus mutans is to the trimethoprim-sulfamethoxazole medicaments insensitive; The capable property of medicine contrast of A hole is red, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is to the medium sensitivity of trimethoprim-sulfamethoxazole medicine; The capable property of medicine contrast of A hole is yellow, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is insensitive to the trimethoprim-sulfamethoxazole medicine;
The capable property of medicine contrast of clindamycin A hole is red, and the capable property of medicine contrast of B hole is red, shows that this Streptococcus mutans is to the clarithromycin medicaments insensitive; The capable property of medicine contrast of A hole is red, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is to the medium sensitivity of clarithromycin medicine; The capable property of medicine contrast of A hole is yellow, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is insensitive to the clarithromycin medicine.
The capable property of medicine contrast of vancomyein A hole is red, and the capable property of medicine contrast of B hole is red, shows that this Streptococcus mutans is to the vancomyein medicaments insensitive; The capable property of medicine contrast of A hole is red, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is to the medium sensitivity of vancomyein medicine; The capable property of medicine contrast of A hole is yellow, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is insensitive to the vancomyein medicine.
The capable property of medicine contrast of Roxithromycin A hole is red, and the capable property of medicine contrast of B hole is red, shows that this Streptococcus mutans is to the Roxithromycin medicaments insensitive; The capable property of medicine contrast of A hole is red, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is to the medium sensitivity of Roxithromycin medicine; The capable property of medicine contrast of A hole is yellow, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is insensitive to the Roxithromycin medicine.
The capable property of medicine contrast of Foromacidin A hole is red, and the capable property of medicine contrast of B hole is red, shows that this Streptococcus mutans is to the Spiramycin Base medicaments insensitive; The capable property of medicine contrast of A hole is red, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is to the medium sensitivity of Spiramycin Base medicine; The capable property of medicine contrast of A hole is yellow, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is insensitive to the Spiramycin Base medicine.
The capable property of medicine contrast of ceftriaxone A hole is red, and the capable property of medicine contrast of B hole is red, shows that this Streptococcus mutans is to the ceftriaxone medicaments insensitive; The capable property of medicine contrast of A hole is red, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is to the medium sensitivity of ceftriaxone medicine; The capable property of medicine contrast of A hole is yellow, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is insensitive to the ceftriaxone medicine.
The capable property of medicine contrast of sulperazone A hole is red, and the capable property of medicine contrast of B hole is red, shows that this Streptococcus mutans is to the Spiramycin Base medicaments insensitive; The capable property of medicine contrast of A hole is red, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is to the medium sensitivity of Spiramycin Base medicine; The capable property of medicine contrast of A hole is yellow, and the capable property of medicine contrast of B hole is yellow, shows that this Streptococcus mutans is insensitive to the Spiramycin Base medicine.
The Streptococcus mutans Rapid identification of the utility model is cultivated the susceptibility test kit, and simple to operate and sense cycle weak point has solved existing Streptococcus mutans and identified the problem that wastes time and energy that exists in the cultural method.
Claims (2)
1. a Streptococcus mutans Rapid identification is cultivated the susceptibility test kit; Comprise test kit part and drug sensitive plate part; It is characterized in that; Said test kit partly comprises box body, and the petridish that places the intravital ultrapure water reagent bottle of box, Yellow Protopet 2A reagent bottle, saline water reagent bottle, interior dress Streptococcus mutans Rapid identification substratum; Said drug sensitive plate partly comprises plate body and Ban Gai; Said plate body is provided with negative hole, the positive detection hole detected; Detecting hole and positive detection hole corresponding to feminine gender is provided with the corresponding delegation's property of medicine in two row property of medicine contrast holes and positive detection hole and contrasts the hole and detection of drugs is housed in respectively.
2. Streptococcus mutans Rapid identification according to claim 1 is cultivated the susceptibility test kit; It is characterized in that the property of medicine contrast hole of in corresponding delegation property of medicine contrast hole, said positive detection hole is respectively penicillium mould, Oxacyclotetradecane,erythromycin deriv, Azythromycin, tsiklomitsin, levofloxacin, trimethoprim-sulfamethoxazole, clindamycin, vancomyein, Roxithromycin, Spiramycin Base, ceftriaxone or sulperazone being housed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220108791 CN202543209U (en) | 2012-03-21 | 2012-03-21 | Quick identifying culturing drug sensitivity kit for streptococcus mutans |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220108791 CN202543209U (en) | 2012-03-21 | 2012-03-21 | Quick identifying culturing drug sensitivity kit for streptococcus mutans |
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| CN202543209U true CN202543209U (en) | 2012-11-21 |
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| CN 201220108791 Expired - Fee Related CN202543209U (en) | 2012-03-21 | 2012-03-21 | Quick identifying culturing drug sensitivity kit for streptococcus mutans |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107299127A (en) * | 2017-08-24 | 2017-10-27 | 惠州市阳光生物科技有限公司 | The hammer dientification of bacteria of B races and susceptibility detection card, the hammer dientification of bacteria of B races and susceptibility detection kit and its application method |
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2012
- 2012-03-21 CN CN 201220108791 patent/CN202543209U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107299127A (en) * | 2017-08-24 | 2017-10-27 | 惠州市阳光生物科技有限公司 | The hammer dientification of bacteria of B races and susceptibility detection card, the hammer dientification of bacteria of B races and susceptibility detection kit and its application method |
| CN107299127B (en) * | 2017-08-24 | 2020-11-06 | 惠州市阳光生物科技有限公司 | Group B streptococcus identification and drug sensitivity detection card, group B streptococcus identification and drug sensitivity detection kit and use method thereof |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121121 Termination date: 20130321 |