CN201933086U - Micro-volume testing plate for primarily testing fast drug susceptibility and bacteria types of combination of tuberculosis and anti-acid mycobacterium - Google Patents
Micro-volume testing plate for primarily testing fast drug susceptibility and bacteria types of combination of tuberculosis and anti-acid mycobacterium Download PDFInfo
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- CN201933086U CN201933086U CN2011200007799U CN201120000779U CN201933086U CN 201933086 U CN201933086 U CN 201933086U CN 2011200007799 U CN2011200007799 U CN 2011200007799U CN 201120000779 U CN201120000779 U CN 201120000779U CN 201933086 U CN201933086 U CN 201933086U
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Abstract
A micro-volume testing plate for primarily testing fast drug susceptibility and bacteria types of the combination of tuberculosis and anti-acid mycobacterium is in a shape of rectangle. Cylinder-cone-shaped micro-culture holes are equally arranged on the plate and at least more than two rows and five lines. The diameter of the bottom of each cylinder-cone-shaped micro-culture hole is only one millimeter. The bottom of each micro-culture hole is provided with a light-transmitted window which has a flat mirror plane and is similar to a glass slide. A top cover is arranged at the upper portion of each cylinder-cone-shaped micro-culture hole. The top is provided with a protruded sealing circle which is embedded inside the hole when the top cover covers on the hole. Various drug-resistant strains can be tested at a time. The results of drug susceptibility and the test of bacteria types can be provided.
Description
Technical field
The utility model relates to clinical and microorganism detection articles for use technical field.Fast trace is cultivated, and detects tuberculosis and the antiacid mycobacterium susceptibility to various antibacterials continuously in real time.
Background technology
Per three philtrums in the whole world just have a people to infect latent tuberculosis germ (being tuberculosis bacteria).Only when these bacteriums became reactivity, people just suffered from tuberculosis.Tuberculosis can adopt treat the course of treatment of four kinds of standards or a line antitubercular agent usually.If these medicines are abused or miscarriage, then can develop into anti-multiple medicines tuberculosis (MDR-TB).Longer with the anti-multiple medicines tuberculosis of two wires pharmacological agent required time, expensive more, and produce more side effects.Also abused or miscarriage and when therefore also becoming invalid at these two wires medicines, can be developed into extensive resistance tuberculosis.It is essential that the clinician recognizes chemical sproof possibility, and utilize drug sensitive experiment result accurately may be provided in early days, so that formulate effective treatment plan as early as possible.If in phlegm, find tuberculosis bacteria, can make diagnosis, but this result can not distinguish this tubercule bacillus to which kind of medicaments insensitive or resistance.Therefore to estimate drug susceptibility, must cultivate and detect bacterium in suitable laboratory.
The mycobacterium tuberculosis growth is very slow.Mycobacterium is cultivated still traditional method (hereinafter to be referred as " rules " method), i.e. absolute concentration method and the scaling method (the national Clinical Laboratory working specification third edition 2,006 916～917) of adopting of drug sensitive test more at present.On tradition improvement sieve (L-J) family name solid medium, cultivate one's ability through 4～8 weeks and grow bacterium colony, could obtain drug sensitivity tests through 4 weeks again.By this way tuberculosis, especially extensive resistance tuberculosis are carried out final diagnosis, may need 6-16 week.Shorten the diagnosis required time, press for new rapid tuberculosis diagnosis instrument.
For the ease of distinguishing different resistant tuberculosis, The World Health Organization (WHO) is divided into following five kinds to resistant tuberculosis:
Single resistant tuberculosis (DR-TB): in vitro tests confirms that the mycobacterium tuberculosis of tubercular's infection is to a kind of antitubercular agent resistance.
Many resistant tuberculosis (PDR-TB): in vitro tests confirms that the mycobacterium tuberculosis of tubercular's infection is not to comprising the two or more antitubercular agent resistances of vazadrine and Rifampin simultaneously;
Anti-multiple medicines tuberculosis (MDR-TB): in vitro tests confirms that the mycobacterium tuberculosis of tubercular's infection is at least to vazadrine in the line antitubercular agent and Rifampin resistance.
Extensive resistant tuberculosis (XDR-TB): in vitro tests confirms mycobacterium tuberculosis that the tubercular infects at least to outside the vazadrine and Rifampin resistance in the line antitubercular agent, also simultaneously to the arbitrary fluoroquinolones resistance in the arbitrary injection class medicine in kantlex, amikacin, the capromycin and Ofloxacine USP 23, levofloxacin, the Moxifloxacin.
Full resistant tuberculosis (TDR-TB): in vitro tests confirms that the mycobacterium tuberculosis that the tubercular infects all produces resistance to all line antitubercular agents and all two wires antitubercular agent Ofloxacine USP 23s that carry out drug sensitive test, kantlex, amikacin, capromycin, ethionamide, Protionamide, seromycin, para-aminosalicylic acid etc.
The product that microwell plate is used for microorganism medicine sensitive and evaluation is a lot, and by innovative design, Liu Zhenghua etc. have proposed to be exclusively used in the bacterium microtest plate (ZL 2,007 2 0040393.4) that the mycobacterium tuberculosis susceptibility is identified.For improving the practicality of drug sensitive test operation, further improve the bacterium microtest plate, design can be carried out the drug sensitivity test culture plate of a line and two wires antituberculosis drugs thing and non-rules medicine, once filters out the sensitive medicaments to Resistant strain, instructs medicine for treatment.
The utility model content
The purpose of this utility model is: propose a kind of tuberculosis and antiacid mycobacterium combination quick medicine-sensitive and bacterial type preliminary evaluation trace detection plate, it has simple in structure, detects purpose fast and accurately.
The technical solution of the utility model is: tuberculosis and antiacid mycobacterium combination quick medicine-sensitive and bacterial type preliminary evaluation trace detection plate, has cylinder taper trace culture hole, through the design permutation and combination, the vacuum lyophilization bag is by antitubercular agent and cultivate based in each hole with various medicines.
Special-purpose microtest plate: tuberculosis and antiacid mycobacterium combination quick medicine-sensitive and bacterial type preliminary evaluation trace detection plate, check-out console is a rectangular plate, has evenly distributed cylinder taper trace culture hole on the plate, at least greater than 7 row, 10 row, especially 8 row 12 are listed as, diameter only has 1 mm at the bottom of the cylinder taper trace culture hole hole, at the bottom of the hole and have an optical transmission window of the smooth and slide glass sample of minute surface, at the bottom of the hole also as the view port of gathering image, corresponding each cylinder taper trace culture hole top is equipped with loam cake, on cover and be provided with an outstanding sealing-ring, loam cake covers the back sealing-ring and embeds in the aperture, prevents that effectively liquid is excessive in the hole; Need the bacterium cultivated by natural subsidence behind the application of sample, concentrate on view port at the bottom of the hole, view port is observed at the bottom of the hole, improves observation efficiency.
Especially be provided with the plate lid corresponding with check-out console, the correspondence position that plate covers in cylinder taper trace culture hole is equipped with loam cake, i.e. the incorporate formation plate lid of all loam cakes.
Every cylinder taper trace culture hole volume is 0.35 ml, has sample and concentrates at the bottom of the optical aperture and closed cover.After the aseptically process, each hole adds substratum and relative medicine on request in the plate, builds loam cake, vacuum lyophilization, and the aseptic condition sealing is preserved.
The beneficial effects of the utility model are: use simple: directly bacteria suspension is added to contain in the freeze-dried drug trace culture hole and can finish vaccine program.Save cost: trial drug that micro plate uses and substratum seldom, every hole only 0.1ml(only be well-established law 1/25～1/40), saved a large amount of reagent costs.Experiment is quick: observe the propagation of single bacterium and the propagation form of small bacterioflora by surpassing thousand times of amplification systems continuously, judge pharmaceutically-active result in early days.Efficiently: a small amount of bacterium liquid can carry out whole medicine drug sensitive tests, reduces the dependence to the bacterium amount.Science compatibility: comprise conventional tuberculosis one line, two wires medicine and other antitubercular agents and penicillin medicine, once detect single resistant tuberculosis (DR-TB), anti-multiple medicines tuberculosis (MDR-TB), extensive resistant tuberculosis (XDR-TB) and full resistant tuberculosis (TDR-TB), it is clinical in drug sensitivity tests adjustment therapeutic regimen to provide drug sensitivity tests to be convenient to.Simple in structure, detect quick and precisely.
Description of drawings
Fig. 1 is the utility model structural representation.
Embodiment
The circle of arranging on Fig. 1 rectangle check-out console is a cylinder taper trace culture hole, and check-out console or/and plate cover row and be composed of numeral or alphabetical sequence number with row.The check-out console (incorporate) that plate covered and arranged each cylinder taper trace culture hole all can prepare by the injection moulding mode.Add experimental concentration tubercule bacillus bacterium liquid in each cylinder taper trace culture hole during use, cultivate in 37 ℃ of constant incubators.Observed every 24 hours after 3 days.By surpassing thousand times of amplification systems, gather the bacterial growth image, and compare with negative and positive control wells image, the similar resistance that is judged to the positive control hole, on the contrary be responsive.
Be built-in with through the vacuum lyophilization bag by antitubercular agent and substratum in cylinder taper trace culture hole.Described bag is comprised drug sensitive test combination, line antitubercular agent combination, the combination of two wires antitubercular agent by antitubercular agent, and non-rules medicine tuberculosis combination and bacterial type are identified combination.Described bag is the medicine that once detects single resistance tubercule bacillus, anti-multiple medicines tubercule bacillus, extensive resistance tubercule bacillus and full resistance tuberculosis by antitubercular agent.
Bag is comprised that by antitubercular agent drug sensitive test combination is a bag by a conventional line and two wires antitubercular agent, bag by penicillins, carbapenems and Clavulanic Potassium, aminoglycoside, cephalosporin, quinolone and fluoroquinolones, aminoglycoside, macrolide antibiotics and all through evidence to tuberculosis and the effective medicine of antiacid mycobacterium.
The utility model tuberculosis and antiacid mycobacterium combination quick medicine-sensitive and bacterial type preliminary evaluation trace detection plate under the condition of the cylinder taper trace culture hole quantity with 8 row, 12 row (by putting different medicines and substratum) can be finished drug sensitive test combination, line antitubercular agent combination, the combination of two wires antitubercular agent by a plate, and non-rules medicine tuberculosis combination and bacterial type are identified the work of combination.
Substratum: improvement Michaelis (Middleblook) 7H-9 liquid nutrient medium.
Drug sensitive test medicine: drug level ug/ml
01, vazadrine (H) 0.05～5000
02, Rifampin (R) 0.05～500
03, Streptomycin sulphate (S) 0.5～500
04, than carboxamide dihydrochloride (Z) 1～5000
05, Tibutol (E) 0.5～500
06, to amino bigcatkin willow (PAS) 0.1～500
07, kantlex (Km) 0.5～500
08, amikacin (AMK) 0.1～10000
09, capromycin (Cm) 0.1～500
10, seromycin (Cs) 0.1～500
11, Ofloxacine USP 23 (Ofx) 0.1～500
12, levofloxacin (Lfx) 0.1～500
13, ethionamide (Eto) 0.1～500
14, Protionamide (Pto) 0.1～500
15, Moxifloxacin (Mfx) 0.1～500
16, Ciprofloxacin (Cfx) 0.1～500
17, Gatifloxacin (Gfx) 0.1～500
18, Sparfloxacin (Sfx) 0.1～500
19, benzylpenicillin sodium (PNa) 0.1～5000
20, cefoxitin (Cfx) 1～5000
21, cefotaxime (Cft) 0.1～5000
22, amoxycilline Trihydrate bp (Amx) 0.1～5000
23, meropenem (MP) 0.1～5000
24, imipenum (IPM) 0.1～5000
25, Clavulanic Potassium (C) 1～500
26, erythromycin (ERY) 1～500
27, Roxithromycin (Rox) 1～500
28, clarithromycin (CLM) 1～500
29, tobramycin (TM) 0.1～500
30, doxycycline (DOX) 0.1～500
31, metronidazole (MDE) 1～5000
32, tinidazole (TNE) 1～5000
Thiophene-2-carboxylic acid hydrazides (TCH) 1～5000
To nitro-phenylpropionic acid sodium (PNB) 1～5000
Design susceptibility test combinations: line medicine combination, the combination of two wires medicine, the preliminary evaluation combination once detects single resistance tubercule bacillus, anti-multiple medicines tubercule bacillus, extensive resistance tubercule bacillus and full resistance tuberculosis.Bag is used for tuberculosis and antiacid mycobacterium drug sensitive test by penicillins, Clavulanic Potassium and macrolide antibiotics.
For example: the A1-A5 culture hole is line medicine combination commonly used, the medicine combination of A6-A12 E1-3 two wires commonly used, E4-12 G1-7 resistant organism and non-tuberculous mycobacteria medication combination, the combination of G8-G9 preliminary evaluation.
Judge: the single resistance of only a kind of medicine resistance of H/R
H/R resistance+other a kind of lines, the many resistances of two wires medicine resistance
The anti-multiple medicines of H+R resistance
The extensive resistance of H+R+Km/Am/Cm+Ofx/Lfx/Mfx
A1-12+E1-3 is the full resistance of resistance.
Claims (4)
1. tuberculosis and antiacid mycobacterium identification and susceptibility trace detection plate, it is characterized in that check-out console is a rectangular plate, has evenly distributed cylinder taper trace culture hole on the plate, cylinder taper trace culture hole is at least greater than 2 row, 5 row, diameter only has 1 mm at the bottom of the cylinder taper trace culture hole hole, at the bottom of the hole and have an optical transmission window of the smooth and slide glass sample of minute surface, corresponding each cylinder taper trace culture hole top is equipped with loam cake, on cover and be provided with an outstanding sealing-ring, loam cake covers the back sealing-ring and embeds in the aperture.
2. tuberculosis according to claim 1 and antiacid mycobacterium identification and susceptibility trace detection plate is characterized in that cylinder taper trace culture hole is 8 row, 12 row.
3. tuberculosis according to claim 1 and 2 and antiacid mycobacterium identification and susceptibility trace detection plate, it is characterized in that being provided with the plate lid corresponding with check-out console, be the integrated plate lid of loam cake, the correspondence position that plate covers in cylinder taper trace culture hole is equipped with loam cake.
4. tuberculosis according to claim 1 and 2 and antiacid mycobacterium identification and susceptibility trace detection plate is characterized in that every cylinder taper trace culture hole volume is 0.2～1.0 ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011200007799U CN201933086U (en) | 2011-01-04 | 2011-01-04 | Micro-volume testing plate for primarily testing fast drug susceptibility and bacteria types of combination of tuberculosis and anti-acid mycobacterium |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011200007799U CN201933086U (en) | 2011-01-04 | 2011-01-04 | Micro-volume testing plate for primarily testing fast drug susceptibility and bacteria types of combination of tuberculosis and anti-acid mycobacterium |
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| CN201933086U true CN201933086U (en) | 2011-08-17 |
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| CN2011200007799U Expired - Fee Related CN201933086U (en) | 2011-01-04 | 2011-01-04 | Micro-volume testing plate for primarily testing fast drug susceptibility and bacteria types of combination of tuberculosis and anti-acid mycobacterium |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555569A (en) * | 2013-11-15 | 2014-02-05 | 安徽科技学院 | High-throughput plant transgenosis hygromycin identification box |
| CN111527193A (en) * | 2017-12-13 | 2020-08-11 | 株式会社日立高新技术 | Antibacterial agent introduction plate for bacteriological examination and transparent plate |
| CN114196541A (en) * | 2021-12-24 | 2022-03-18 | 杭州艾名医学科技有限公司 | Culture plate with filtering function suitable for automatic pipetting system |
-
2011
- 2011-01-04 CN CN2011200007799U patent/CN201933086U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555569A (en) * | 2013-11-15 | 2014-02-05 | 安徽科技学院 | High-throughput plant transgenosis hygromycin identification box |
| CN111527193A (en) * | 2017-12-13 | 2020-08-11 | 株式会社日立高新技术 | Antibacterial agent introduction plate for bacteriological examination and transparent plate |
| CN111527193B (en) * | 2017-12-13 | 2024-04-05 | 株式会社日立高新技术 | Antibacterial agent introduction plate for bacterial examination and transparent plate |
| CN114196541A (en) * | 2021-12-24 | 2022-03-18 | 杭州艾名医学科技有限公司 | Culture plate with filtering function suitable for automatic pipetting system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110817 Termination date: 20170104 |