CN103555569A - High-throughput plant transgenosis hygromycin identification box - Google Patents
High-throughput plant transgenosis hygromycin identification box Download PDFInfo
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- CN103555569A CN103555569A CN201310567867.0A CN201310567867A CN103555569A CN 103555569 A CN103555569 A CN 103555569A CN 201310567867 A CN201310567867 A CN 201310567867A CN 103555569 A CN103555569 A CN 103555569A
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- hygromycin
- totomycin
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- base plate
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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Abstract
The invention discloses a high-throughput plant transgenosis hygromycin identification box, and belongs to the field of biotechnology. When transgenic identification is carried out on the present age or offspring of plant transgene, plants and leaves are generally required to be numbered, and then the leaves are sheared and soaked in a hygromycin solution. When the amount of transgenic plants is great, the method has two defects that firstly, the plants and leaves are numbered, so that the time and labor are wasted, and mistakes easily occur; and secondly, under the condition of no special tool, the using amount of hygromycin is large, and the cost is too much since the price of hygromycin is expensive. The high-throughput plant transgenosis hygromycin identification box is used for plant transgenosis identification, and can simplify the operation procedure, thus reducing the working amount, reducing the using amount of hygromycin and saving a large amount of cost.
Description
Technical field
Plant transgene Totomycin is identified a box, belongs to biotechware, says more specifically a kind of special tool that can carry out Totomycin evaluation to a large amount of plant transgene offsprings.
Background technology
When the plant transgene present age or offspring are identified, the sample taking off on plant need be soaked in Totomycin solution, conventionally using culture dish or beaker as container, the required Totomycin solution usage of this kind of method is larger, and holds several testing samples in a container, easily causes and mixes, especially when transfer-gen plant quantity is larger, will produce two problems: the one, number seriatim the container of sample and Totomycin, waste time and energy, easily make mistakes; The 2nd, Totomycin is expensive, its large usage quantity in the situation that there is no special tool, and cost is more.
Summary of the invention
The problem existing while the object of the invention is for current Totomycin processing, evaluation transgenic plant sample, provide a kind of high-throughput Totomycin that can identify a large amount of transfer-gen plants to identify box, this instrument both can have been removed process when Totomycin soaks, container being numbered from, increased work efficiency; Can save the consumption of Totomycin again, thus reduction of expenditure.
Structure of the present invention is:
1. high-throughput plant transgene Totomycin is identified a box, base plate, lid, consists of, and it is characterized in that, is distributed with some sample caves on base plate.
2. the quantity in the sample cave on the base plate described in is 100.
3. the distribution mode in 100 sample caves on the base plate described in is 10 row 10 row.
4. the sample cave on the base plate described in is cylindrical, and its degree of depth is 1cm, and diameter is 2cm.
5. the ranks in the sample cave on the base plate described in are all used numeral or lexicographic order mark.
6. the evaluation box described in adopts transparent plastics to be made.
Beneficial effect
When the present invention has successfully solved plant transgene and has detected, as various in material, often need plant and blade to be numbered respectively, workload greatly and the problem of easily makeing mistakes, thereby greatly reduce the consumption of Totomycin reagent, saving experimentation cost, increases work efficiency.
Accompanying drawing explanation
Fig. 1 is the floor map that a kind of high-throughput plant transgene Totomycin is identified box.
Fig. 2 is the diagrammatic cross-section that a kind of high-throughput plant transgene Totomycin is identified box.
In figure: 1 base plate, 2 sample caves, 3 lids.
Embodiment
Below in conjunction with the drawings and specific embodiments, one kind of plant thorn embryo transgenosis inoculation special needle is further detailed.
One, a kind of high-throughput plant transgene Totomycin is identified the structure of box:
1. high-throughput plant transgene Totomycin is identified a box, base plate, lid, consists of, and it is characterized in that, is distributed with some sample caves on base plate.
2. the quantity in the sample cave on the base plate described in is 100.
3. the distribution mode in 100 sample caves on the base plate described in is 10 row 10 row.
4. the sample cave on the base plate described in is cylindrical, and the degree of depth is 1cm, and diameter is 2cm.
5. the ranks in the sample cave on the base plate described in are all used numeral or lexicographic order mark.
6. the evaluation box described in adopts transparent plastics to be made.
Two, a kind of high-throughput plant transgene Totomycin is identified the using method of box:
1. the rotaring gene plant blade of needs being identified is cut into 1-2 centimetre of size, and plant leaf is put in the sample cave in identification plate in order.
2. in sample cave, add Totomycin solution, cover lid.
3. after 48 hours, observing blade has or not foxiness to judge the whether positive plant of transfer-gen plant.
Claims (6)
1. high-throughput plant transgene Totomycin is identified a box, base plate, lid, consists of, and it is characterized in that, is distributed with some sample caves on base plate.
2. high-throughput Totomycin according to claim 1 is identified box, it is characterized in that, on described base plate, the quantity in sample cave is 100.
3. high-throughput Totomycin according to claim 1 and 2 is identified box, it is characterized in that, the distribution mode in 100 sample caves on described base plate is 10 row 10 row.
4. high-throughput Totomycin according to claim 1 is identified box, it is characterized in that, the sample cave on described base plate is cylindrical, and the degree of depth is 1cm, and diameter is 2cm.
5. high-throughput Totomycin according to claim 1 is identified box, it is characterized in that, the ranks in the sample cave on described base plate are all used numeral or lexicographic order mark.
6. high-throughput Totomycin according to claim 1 is identified box, it is characterized in that, described evaluation box adopts transparent plastics to be made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310567867.0A CN103555569A (en) | 2013-11-15 | 2013-11-15 | High-throughput plant transgenosis hygromycin identification box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310567867.0A CN103555569A (en) | 2013-11-15 | 2013-11-15 | High-throughput plant transgenosis hygromycin identification box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103555569A true CN103555569A (en) | 2014-02-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310567867.0A Pending CN103555569A (en) | 2013-11-15 | 2013-11-15 | High-throughput plant transgenosis hygromycin identification box |
Country Status (1)
| Country | Link |
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| CN (1) | CN103555569A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105830763A (en) * | 2016-04-12 | 2016-08-10 | 扬州大学 | Application of hygromycin serving as selective agent in wheat transformation |
| CN109874450A (en) * | 2019-01-31 | 2019-06-14 | 浙江大学 | A kind of device and method of high flux screening transgenic homozygous plant |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0183973A1 (en) * | 1984-11-01 | 1986-06-11 | Becton Dickinson and Company | Multiwell tissue culture assembly with features for reduced media evaporation |
| CN200988841Y (en) * | 2006-09-20 | 2007-12-12 | 上海复星佰珞生物技术有限公司 | Microporous plate for microbial detection and medicine sensitive test |
| CN201158658Y (en) * | 2007-09-17 | 2008-12-03 | 上海中正慧东医疗技术有限公司 | Drop composition board for microbe drug sensitivity experiment |
| CN201395598Y (en) * | 2009-03-12 | 2010-02-03 | 山东省医学科学院基础医学研究所 | Sealed type micro porous culture plate |
| CN201933086U (en) * | 2011-01-04 | 2011-08-17 | 南京市胸科医院 | Micro-volume testing plate for primarily testing fast drug susceptibility and bacteria types of combination of tuberculosis and anti-acid mycobacterium |
| CN202139238U (en) * | 2011-07-13 | 2012-02-08 | 珠海美华医疗科技有限公司 | Micropore plate for microbiological assay |
| CN202440518U (en) * | 2012-02-16 | 2012-09-19 | 山东省立医院 | Cell culture plate |
-
2013
- 2013-11-15 CN CN201310567867.0A patent/CN103555569A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0183973A1 (en) * | 1984-11-01 | 1986-06-11 | Becton Dickinson and Company | Multiwell tissue culture assembly with features for reduced media evaporation |
| CN200988841Y (en) * | 2006-09-20 | 2007-12-12 | 上海复星佰珞生物技术有限公司 | Microporous plate for microbial detection and medicine sensitive test |
| CN201158658Y (en) * | 2007-09-17 | 2008-12-03 | 上海中正慧东医疗技术有限公司 | Drop composition board for microbe drug sensitivity experiment |
| CN201395598Y (en) * | 2009-03-12 | 2010-02-03 | 山东省医学科学院基础医学研究所 | Sealed type micro porous culture plate |
| CN201933086U (en) * | 2011-01-04 | 2011-08-17 | 南京市胸科医院 | Micro-volume testing plate for primarily testing fast drug susceptibility and bacteria types of combination of tuberculosis and anti-acid mycobacterium |
| CN202139238U (en) * | 2011-07-13 | 2012-02-08 | 珠海美华医疗科技有限公司 | Micropore plate for microbiological assay |
| CN202440518U (en) * | 2012-02-16 | 2012-09-19 | 山东省立医院 | Cell culture plate |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105830763A (en) * | 2016-04-12 | 2016-08-10 | 扬州大学 | Application of hygromycin serving as selective agent in wheat transformation |
| CN109874450A (en) * | 2019-01-31 | 2019-06-14 | 浙江大学 | A kind of device and method of high flux screening transgenic homozygous plant |
| CN109874450B (en) * | 2019-01-31 | 2023-08-01 | 浙江大学 | Device and method for high-throughput screening of transgenic homozygous plants |
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Application publication date: 20140205 |