CN202415570U - Integrated preparation rack for cell growing on glass slide - Google Patents
Integrated preparation rack for cell growing on glass slide Download PDFInfo
- Publication number
- CN202415570U CN202415570U CN2011205646229U CN201120564622U CN202415570U CN 202415570 U CN202415570 U CN 202415570U CN 2011205646229 U CN2011205646229 U CN 2011205646229U CN 201120564622 U CN201120564622 U CN 201120564622U CN 202415570 U CN202415570 U CN 202415570U
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- Prior art keywords
- cell
- preparation frame
- climbing sheet
- cell climbing
- groove
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Abstract
The utility model discloses an integrated preparation rack for cell growing on glass slide, belonging to the cell culture utensils. The integrated preparation rack consists of a preparation rack base and a preparation rack cover, wherein the preparation rack base has a structure that: the three sides of the upper surface of a rectangular bottom plate are provided with clamp slots formed by connecting upward extension sections and inward extension sections; four support legs are arranged at the bottom of the rectangular bottom plate; the upper surface of the rectangular bottom plate is provided with multiple strip grooves distributed at an equal interval and parallel to each other; a growing slot for the cell to grow on glass slide is arranged on the two upper edges in the long side direction of each strip groove respectively; the growing slots are uniformly distributed at an equal interval in the long side direction of the strip grooves; and the preparation rack cover has a structure that: the three sides of the bottom surface of a rectangular cover plate are provided with outward-extending flanges which are inserted into the clamp slots of the preparation rack base. The integrated preparation rack disclosed by the utility model has the characteristics of good positioning effect for the cell growing on glass slide, convenience for use, etc.
Description
Technical field
The utility model relates to a kind of cell in vitro to be cultivated and the required experiment utensil of correlative study, the integrated preparation frame of cell climbing sheet in especially a kind of high-level efficiency, high quality, the placed Tissue Culture Dish easy and simple to handle.
Background technology
The cell technology of vitro culture is one of important technology of life science researchs such as biology, preclinical medicine.Along with going deep into of research, cell research method and cell detection index are day by day abundant, and the cell of vitro culture preparation and the demand of cell climbing sheet are increased gradually; Simultaneously,, usually need under identical condition, prepare cell and cell climbing sheet, and require cell proterties homogeneous, favourable identity, make to have more comparability between the experimental result in order to guarantee the objectivity of experimental result.But there is defective in various degree in the method for this field employing at present.
Ordinary method be directly place petridish to cell climbing sheet at the bottom of, after cell cultures finished, the cell of collecting cell creep plate and petridish bottom growth was used for the detection of different research and index.But this method has following shortcoming: 1, cell climbing sheet slides at the bottom of slick petridish easily and is overlapping, and the growth conditions of cell on cell climbing sheet can not be unified; 2, in order to reduce overlapping between the cell climbing sheet, cell climbing sheet need disperse to place, and simultaneously, cell climbing sheet has occupied at the bottom of a part of petridish, and therefore, the cell of preparation and cell climbing sheet are all few, is difficult to satisfy the experiment needs; 3, because cell climbing sheet directly with at the bottom of the petridish contacts, and cell can stick to the back side of cell climbing sheet, causes cell in the two-sided growth of cell climbing sheet, influence is observed and subsequent experimental is handled; 4, cell climbing sheet directly places at the bottom of the petridish, combines with the bottom is tight, is difficult to separately, and gripping is difficulty very.In addition, when testing, owing to will relatively waste experiment material and reagent to each cell climbing sheet individual curing with cell climbing sheet; The cell climbing sheet size is generally less, and pick and place, shift, observation etc. must be concentrated, and operation is careful, prevent to drop, press from both sides broken, put upside down pros and cons etc., the experimental implementation process is complicated, difficulty is bigger, time and effort consuming; When the cell climbing sheet of preparation stored, overlapping easily, upset, scratch scratched.
Utility model (ZL200920080719.5) utilizes the support in the preparation box to solve the many problems in above cell climbing sheet preparation and the use cell and the integrated preparation of cell climbing sheet.But, if the cell cultures time is long slightly, because the lubrication of nutrient solution; Cell climbing sheet is fixing not to be fine, and the cell climbing sheet that has skids off from the support two ends easily, influences the quality of cell climbing sheet and lower floor's cultured cells; The cell climbing sheet that has is close together easily each other, causes taking inconvenience.During storage, the problem that also exists cell climbing sheet to come off easily.
The utility model content
The purpose of the utility model is to provide fixing safe, the preparation of a kind of cell climbing sheet to take the integrated preparation frame of cell climbing sheet easily to the problem in the above-mentioned technology.
The purpose of the utility model is achieved in that the integrated preparation frame of a kind of cell climbing sheet, is made up of preparation frame base and preparation frame lid; Preparation frame understructure is: have on three limits of rectangular base plate upper surface by the section of extending upward and the section of extending internally to be connected the draw-in groove that forms; Four legs are arranged at the rectangular base plate bottom; A plurality of strip grooves that are spacedly distributed, are parallel to each other are arranged on the rectangular base plate upper surface; Two upper edge hole places of each strip groove long side direction have the creep plate groove that supplies cell climbing sheet to place respectively, and the creep plate groove equidistantly is uniformly distributed with setting along the strip groove long side direction; Preparation frame lid structure is: have on three limits of rectangle cover plate bottom surface as the outwardly directed flange among the draw-in groove that inserts preparation frame base.
The degree of depth of above-mentioned creep plate groove is more than or equal to the thickness of cell climbing sheet.
Above-mentioned rectangular base thickness of slab 1mm, the section of extending upward and the Duan Junhou 0.5mm that extends internally, creep plate groove depth 0.17mm～0.2mm.
The outer side elevation of above-mentioned preparation frame base is the frosting that is used for mark.
Compared with prior art, this novel beneficial effect is:
1,, can prepare the cell and the cell climbing sheet of different disposal simultaneously in a large number in conjunction with the conventional Tissue Culture Dish that uses.The cell for preparing in the Tissue Culture Dish and creep plate prepare the cell climbing sheet on the frame because cultivate, treating processes is identical, various influence factors such as environment of living in and operating process are consistent, so the character homogeneous, have better comparability;
2, every cell climbing sheet is owing to independent stationary in cultivation is stopped the problem that comes off, is shifted in the creep plate groove of oneself.It is inhomogeneous to have eliminated the cell growth, and nature difference is big, takes the problem of inconvenience;
3, all cell climbing sheets all are fixed on the preparation frame successively, do as a wholely to cultivate, handle, storage etc., easy to operate effectively;
4, the preparation frame also is the storage shelf of cell climbing sheet simultaneously, and a plurality of preparation frame marks are good, stack and vertically put in the special box, and easy storing, it is also convenient to take;
5, the preparation framework is made simply, and is multiple functional, and manufacture craft is ripe, and consumptive material is few, and cost is low;
6, the preparation box can use repeatedly, economizes on resources.
In cell cultivation process; Adopt the utility model to be fixed on independently in the creep plate groove cell climbing sheet is integrated well, stop the problem that comes off, be shifted, make cell climbing sheet character homogeneous, the quality height of preparation; Storage, easy and simple to handle; Significantly reduce labor intensity of operating personnel, conventional efficient is higher, better effects if.
Description of drawings
Fig. 1 is the stereographic map of preparation frame base.
Fig. 2 is the stereographic map of preparation frame lid.
Embodiment
Below in conjunction with accompanying drawing the utility model is described in further detail.
The utility model is made up of preparation frame base 2 and preparation frame lid 3.Fig. 1 illustrates; Preparation frame understructure is: have on three limits of rectangular base plate upper surface by the section of extending upward and the section of extending internally to be connected the draw-in groove 2c that forms; Four leg 2a are arranged at the rectangular base plate bottom; A plurality of strip grooves that are spacedly distributed, are parallel to each other are arranged on the rectangular base plate upper surface, and two upper edge hole places of each strip groove long side direction have the creep plate groove 2b that supplies cell climbing sheet 1 to place respectively, and creep plate groove 2b equidistantly is uniformly distributed with setting along the strip groove long side direction.
Fig. 2 illustrates, and the preparation frame covers 3 structures and is: have on three limits of rectangle cover plate bottom surface as the outwardly directed flange 3a among the draw-in groove 2c that inserts preparation frame base.The lid cross section is " protruding " font, and the base cross section is " recessed " font, and behind lid propelling base, integral body is a square.The degree of depth of creep plate groove 2b is more than or equal to the thickness of cell climbing sheet 1.Rectangular base thickness of slab 1mm, the section of extending upward and the Duan Junhou 0.5mm that extends internally, creep plate groove 2b degree of depth 0.17mm～0.2mm.
Preparation frame base is provided with the draw-in groove that inserts preparation frame lid.Have on the preparation frame lid 3 with the preparation corresponding strip groove of frame base so that insert and take out.Preparation frame chassis outer side facade is the frosting 2d that is used for mark, and preparation frame lid inserts three limit lower part projections of preparation frame lid, can insert tight with it the coincideing of preparation frame base and be integral.Size and various types of Tissue Culture Dish of preparation frame adapt.
Cell climbing sheet can be rectangle or square, thick 0.1mm～0.2mm.The big I of cell climbing sheet is by the required all size such as length of side 4mm～8mm of processing of experiment.It is lattice work that said creep plate prepares frame.Thick 1.5mm, creep plate groove depth 0.2mm, wide 1mm, the width between grid is advisable can just put into cell climbing sheet.Stay the 1mm space between the creep plate groove, make things convenient for picking and placeing of cell climbing sheet, simultaneously, also make the cultivation fluid power circulation of cell climbing sheet upper and lower, guarantee the character homogeneous of nutrient solution.Creep plate prepares frame top and is provided with the draw-in groove that inserts preparation frame lid, the high 0.5mm of groove, dark 0.5mm.The thick 0.5mm of groove top bars.Four legs are positioned on four angles of base high 0.5mm.Preparation frame base side is the frosting that is used for mark, and the preparation frame covers thick 1mm, on have and the corresponding lattice work of preparation frame base, can cooperate with base cell climbing sheet is fixed in the creep plate groove.Preparation frame lid inserts three limit the latter half projections of preparation frame lid, and thick 0.5mm can insert preparation frame base and tightly coincide with it and be integral.
Creep plate prepares frame can process length of side 4cm～10cm various sizes according to the Tissue Culture Dish model of inserting.Creep plate prepares the frame base, and available mould Integratively cast forms with lid.The creep plate support just can be placed in the common petridish, can make full use of existing resource like this.Creep plate prepares frame and processes by stainless steel or by the transparent material of highdensity high temperature resistant, acid and alkali-resistance, organic solvent-resistant.
In the such scheme, after cell cultures was accomplished, creep plate prepared frame and can integral body puts into stationary liquid and fix, and then, a plurality of creep plates prepare frame can vertically put into the similar special Storage Box 5 of Glass carrier box, puts-20 ℃ of preservations, and supplying in the future, experimental study uses.
Embodiment 1:
Cell climbing sheet is positioned over creep plate prepares in the creep plate groove of frame, insert creep plate and prepare the frame lid, put into the Tissue Culture Dish that adapts; Add an amount of cell suspension, cover the petridish lid, cell is because action of gravity; Can be layered at the bottom of cell climbing sheet front (top) and the petridish, then adherent growth.Cell in a plurality of petridish can apply different processing.After cultivating end, creep plate is prepared frame from petridish, take out, place other discarded petridish; Adding there was not creep plate to prepare the liquid-solid cell of deciding on the cell climbing sheet of cell fixation of frame, and cell fixation discards cell fixation liquid after finishing; Creep plate is prepared the frame marked; Place Storage Box ,-20 ℃ of cryopreservation supply various experiments such as HE dyeing, ICC, ISH, FISH to handle and use.The cell of petridish bottom growth can collect by the method digestion of routine, gets into experiment flows such as nucleic acid, protein preparation.
Claims (4)
1. the integrated preparation frame of cell climbing sheet is characterized in that, is made up of preparation frame base (2) and preparation frame lid (3); Preparation frame understructure is: have on three limits of rectangular base plate upper surface by the section of extending upward and the section of extending internally to be connected the draw-in groove (2c) that forms; Four legs (2a) are arranged at the rectangular base plate bottom; A plurality of strip grooves that are spacedly distributed, are parallel to each other are arranged on the rectangular base plate upper surface; Two upper edge hole places of each strip groove long side direction have the creep plate groove (2b) that supplies cell climbing sheet (1) to place respectively, and creep plate groove (2b) equidistantly is uniformly distributed with setting along the strip groove long side direction; Preparation frame lid (3) structure is: have on three limits of rectangle cover plate bottom surface as the outwardly directed flange (3a) among the draw-in groove (2c) that inserts preparation frame base.
2. the integrated preparation frame of cell climbing sheet according to claim 1 is characterized in that the degree of depth of said creep plate groove (2b) is more than or equal to the thickness of cell climbing sheet (1).
3. the integrated preparation frame of cell climbing sheet according to claim 2 is characterized in that, said rectangular base thickness of slab 1mm, the section of extending upward and the Duan Junhou 0.5mm that extends internally, creep plate groove (2b) degree of depth 0.17mm ~ 0.2mm.
4. according to claim 1 or the integrated preparation frame of 2 or 3 described cell climbing sheets, it is characterized in that the outer side elevation of said preparation frame base is the frosting that is used for mark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011205646229U CN202415570U (en) | 2011-12-30 | 2011-12-30 | Integrated preparation rack for cell growing on glass slide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011205646229U CN202415570U (en) | 2011-12-30 | 2011-12-30 | Integrated preparation rack for cell growing on glass slide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202415570U true CN202415570U (en) | 2012-09-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011205646229U Expired - Fee Related CN202415570U (en) | 2011-12-30 | 2011-12-30 | Integrated preparation rack for cell growing on glass slide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202415570U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723238A (en) * | 2017-09-29 | 2018-02-23 | 东北大学 | A kind of cell culture apparatus and its application |
| CN114369536A (en) * | 2022-02-24 | 2022-04-19 | 中南大学湘雅医院 | Cell climbing sheet culture device and application method thereof |
-
2011
- 2011-12-30 CN CN2011205646229U patent/CN202415570U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723238A (en) * | 2017-09-29 | 2018-02-23 | 东北大学 | A kind of cell culture apparatus and its application |
| CN107723238B (en) * | 2017-09-29 | 2020-07-28 | 东北大学 | Cell culture device and application thereof |
| CN114369536A (en) * | 2022-02-24 | 2022-04-19 | 中南大学湘雅医院 | Cell climbing sheet culture device and application method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120905 Termination date: 20121230 |