CN201424477Y - Integrated preparation box for cell and cell climbing sheet - Google Patents
Integrated preparation box for cell and cell climbing sheet Download PDFInfo
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- CN201424477Y CN201424477Y CN2009200807195U CN200920080719U CN201424477Y CN 201424477 Y CN201424477 Y CN 201424477Y CN 2009200807195 U CN2009200807195 U CN 2009200807195U CN 200920080719 U CN200920080719 U CN 200920080719U CN 201424477 Y CN201424477 Y CN 201424477Y
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- cell
- climbing sheet
- cell climbing
- box
- creep plate
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Abstract
The utility model relates to an integrated preparation box for cells and cell climbing sheets. The integrated preparation box comprises a preparation box and cell climbing sheets, as well as a cell climbing sheet support positioned in the preparation box; wherein support legs are arranged at the bottom of the square frame shaped support base seat, a plurality of cell climbing sheets fixing bars are arranged on the support base seat at equal intervals in parallel, the contracted parts of the H-shaped cross sections of the fixing bars form cell climbing sheet accommodating grooves, and the cellclimbing sheet accommodating groove on two adjacent fixing bars is the position for accommodating the cell climbing sheet. The integrated preparation box has the advantages that the experiment efficiency is high, the effect is better, the materials and the reagent are better saved, the operation is simple, and the labor intensity of the operator is obviously reduced.
Description
Technical field
The utility model relates to a kind of cell in vitro to be cultivated and the required experiment apparatus of correlative study, especially a kind of high-level efficiency, high quality, cell and the integrated preparation box of cell climbing sheet easy and simple to handle.
Background technology
Can get rid of the influence of many interfering factorss of testing in the body as research object with the cell of vitro culture, using various processing factors handles culturing cell and also becomes easy, simultaneously, adopt various technology and method to come pair cell to observe and analyze also more convenient.Therefore, cell culture technology is one of important technology of life science researchs such as biology, preclinical medicine.Along with going deep into of research, cell research method and cell detection index are day by day abundant, and the cell of vitro culture preparation and the demand of cell climbing sheet are increased gradually; Simultaneously,, usually need under identical condition, prepare cell and cell climbing sheet, make cell proterties homogeneous, favourable identity, have more comparability between the experimental result in order to guarantee the objectivity of experimental result.But there is defective in various degree in the method for this field employing at present.
Usual method be a plurality of cell climbing sheets are directly placed culture dish at the bottom of, after cell cultures finished, the cell of collecting cell creep plate and culture dish bottom growth was used for different research methods and different indexs detects.But this method has following shortcoming: 1. cell climbing sheet slides at the bottom of slick culture dish easily and is overlapping, and the growth conditions of cell on cell climbing sheet can not be unified; 2. in order to reduce overlapping between the cell climbing sheet, cell climbing sheet need disperse to place, and simultaneously, cell climbing sheet has occupied at the bottom of a part of culture dish, and therefore, the cell of preparation and cell climbing sheet are all few, is difficult to satisfy the experiment needs; 3. because cell climbing sheet directly with at the bottom of the culture dish contacts, and cell can stick to the back side of cell climbing sheet, causes cell in the two-sided growth of cell climbing sheet, influence is observed and subsequent experimental is handled; 4. cell climbing sheet directly places and causes that cell climbing sheet and bottom are tight to be combined at the bottom of the culture dish, is difficult to separately, and the cell climbing sheet gripping is difficulty very.
The method that also has is to make a plurality of orderly grooves at the bottom of the culture dish, or places the slide glass plate (CN1928063A) with a plurality of orderly grooves in culture dish, and these grooves can grown cells or held cell climbing sheet.Like this, can prepare cell and cell climbing sheet simultaneously, part has solved cell climbing sheet and has slided and the eclipsed problem.But also have the following disadvantages: 1. prepared cell and cell climbing sheet quantity are also limited; 2. cell climbing sheet since at the bottom of the back side and the culture dish or the slide glass plate directly contact, cell equally can two-sided growth; 3. cell climbing sheet is small and light, may be rushed out the groove at place or floating when adding nutrient solution, also might produce the overlapping and uneven problem of cell growth of creep plate; 4. cell climbing sheet directly places at the bottom of the culture dish or causes cell climbing sheet gripping difficulty on the slide glass plate too.Therefore, the benefit of this method is few, and practical significance is very not big.
In addition, when experimentizing, owing to will relatively waste experiment material and reagent to each cell climbing sheet individual curing with cell climbing sheet; The cell climbing sheet size is generally less, and pick and place, shift, observation etc. must be concentrated, and operation is careful, prevent to drop, press from both sides broken, put upside down pros and cons etc., experimental implementation process complexity, difficulty is bigger, time and effort consuming; When the cell climbing sheet of preparation stored,, upset overlapping for preventing, scratch scratched, and often need special storing unit, have also increased experimental cost.Therefore, these problems also need to solve.
The utility model content
The purpose of this utility model is to provide a kind of integrated preparation box of a large amount of character homogeneous, high-quality cell and cell climbing sheet that can prepare simultaneously at the problem in the above-mentioned technology, so that conventional efficient is higher, better effects if, material and reagent are more saved, operate also easylier, significantly reduce labor intensity of operating personnel.
This novel purpose is achieved in that a kind of cell and the integrated preparation box of cell climbing sheet, comprise the cultivation box, cell climbing sheet, also have as the creep plate support that is positioned in the described cultivation box: the bottom of the bracket base of square frame shape is provided with leg, the bracket base equal intervals, be fixed with many creep plate fixed strips abreast, the narrowed portion of " worker " shaped sections of this fixed strip forms the creep plate standing groove, and the creep plate standing groove on adjacent two fixed strips is for placing the position of cell climbing sheet.
Compared with prior art, this novel beneficial effect is:
1, can simultaneously a large amount of preparation property homogeneous, the cell and the cell climbing sheet of reliable quality different treatment.Cell for preparing in the same preparation box and cell climbing sheet are owing to cultivation, treating processes integrate simultaneously and carry out, various influence factor unanimities such as environment of living in and operating process, so the character homogeneous, various research methods and index detected result have better comparability;
2, cell climbing sheet is owing to be in vacant state in cultivation, and cell can't adhere to its back side (following) and grow, and therefore, has only its front (top) can grown cell, has solved the problem of the two-sided growth of cell in the past.Simultaneously, also solved cell climbing sheet in the past and be difficult to separate the problem of cell climbing sheet gripping difficulty because of tightly combining with slide glass plate at the bottom of the culture dish or at the bottom of the culture dish;
3, because in the culturing process, cell climbing sheet is thrown on the creep plate support in an orderly manner, eliminated that cell climbing sheet was overlapped in operation easily in the past, the cell growth is inhomogeneous, the problem that nature difference is big;
4, the cultivation of cell climbing sheet, fixing, store all always on the creep plate support, reduced operations such as cell climbing sheet picks and places, moves, reduced in the past cell climbing sheet complex operation, overlapping, scratch easily, broken, obscure disadvantages such as pros and cons, waste experiment material and reagent into pieces;
5, the cell climbing sheet of multiple different treatment can stick on common slide glass or the special creep plate brassboard together, is integrated into the cell climbing sheet array, dyes simultaneously, processing such as hybridization.Handle with in the past monolithic and to compare, unified operating process, make the suffered processing of every cell climbing sheet more consistent, experimental result is more objective; Simultaneously, also simplify operation, saved the consumption of reagent such as antibody, staining agent and associated materials, economical and efficient;
6, preparation box simple structure is multiple functional, the manufacture craft maturation, and consumptive material is few, and cost is low; The preparation box also is the Storage Box of cell climbing sheet simultaneously, no longer needs special Storage Box; The preparation box can use repeatedly, economizes on resources.
Description of drawings
Fig. 1 is the sectional view of the integrated preparation box of this novel cell and cell climbing sheet;
Fig. 2 is the vertical view (dissecing lid) of Fig. 1;
Fig. 3 is the stereographic map of creep plate support shown in Figure 1.
Embodiment
The utility model is described in further detail below in conjunction with accompanying drawing.
Fig. 1 Fig. 2 illustrates, this novel cultivation box that comprises, cell climbing sheet 4, also have as the creep plate support that is positioned in the described cultivation box: the bottom of the bracket base 2 of square frame shape is provided with leg 2a, bracket base 2 equal intervals, many creep plate fixed strips 1 have been arranged in parallel, the groove that the narrowed portion of " worker " shaped sections of this fixed strip forms is a creep plate standing groove 3, and the creep plate standing groove 3 on adjacent two fixed strips is for placing the position of cell climbing sheet 4.
Cell climbing sheet can be shapes such as square or circle, thick 0.1～0.2mm.The big I of cell climbing sheet is by the required various sizes of making of experiment, and the length of side or diameter are all sizes such as 4～8mm.Described creep plate support is that a plurality of cross sections are on the bracket base of creep plate fixed strip of " worker " font and the square frame shape that is listed in four pin.Each wide 2mm of creep plate fixed strip " worker " font top and the bottom (upper and lower part all is a lath), thick 1mm, the wide 1mm of middle narrowed portion, thick 0.2mm.The groove that creep plate fixed strip narrowed portion forms is the creep plate standing groove, groove depth 0.5mm, wide 0.2mm (X that refers to " worker " font deeply to, wide finger Y to), two relative creep plate standing grooves of creep plate fixed strip are the position of horizontal positioned cell climbing sheet, and the width between groove is advisable can just put into cell climbing sheet.Stay the 1mm space between cell climbing sheet, make things convenient for picking and placeing of cell climbing sheet, simultaneously, the nutrient solution of cell climbing sheet upper and lower can be circulated, guarantee the character homogeneous of nutrient solution.The creep plate standing groove is a matte surface, makes cell climbing sheet place into the back stationkeeping, anti-sliding stop.Lath two ends, creep plate fixed strip " worker " shaped sections top respectively have the 30-60 degree interior to oblique angle 8, can make things convenient for picking and placeing of cell climbing sheet.High 1～the 2mm of square frame shape bracket base, four legs are positioned on four angles of base high 1～3mm.The bracket base square frame can be made the length of side 4～10cm (high 1～2mm, various sizes such as wide 2～3mm) according to the amount of cell, the cell climbing sheet of preparation once.The creep plate support can form with the cast of mould Integratively.The creep plate support is placed on a square or circular a cultivation in the box as common culture dish, cultivating box is made up of the length of side 4～10cm cultivation box body and cultivation lid, box body and creep plate support adapt, square is cultivated cell climbing sheet bracket lip that the box side ratio the placed 6～10mm that grows up, the circular box of cultivating is advisable can just put down the creep plate support, can make things convenient for picking and placeing of creep plate support like this.Base thickness 1～the 2mm of box body.Cultivate the length of side of lid or big 6～10mm that the diameter ratio is cultivated box body.The circular box of cultivating can replace with existing culture dish, can make full use of existing resource like this.Cell climbing sheet, creep plate support and cultivation box are made by the transparent material of high temperature resistant, acid and alkali-resistance, organic solvent-resistant.
In the such scheme, after described cell climbing sheet preparation is finished, can place and carry out HE dyeing, immunocytochemistry (immunocytochemistry on common slide glass or a kind of creep plate brassboard, ICC), in situ hybridization (In situhybridization, ISH), fluorescence in situ hybridization (fluorescence in situ hybridization, handle by FISH) etc. various experiments.The creep plate brassboard is grown 6～9cm by required the making of experiment, wide 2～4cm all size, and the wide part of positive (top) end 2cm is made the frosting that is used for mark, and the cell climbing sheet that rest part is made a plurality of ordered arrangement that adapt with cell climbing sheet adheres to groove.Creep plate adhesion bottom land plane is in or is lower than the brassboard plane, and groove bottom is in or is lower than the brassboard plane, and groove depth is identical with cell climbing sheet thickness, and groove shape and size are identical with cell climbing sheet, and groove is 0.2～0.3mm at interval.This creep plate brassboard is made by the transparent material of high temperature resistant, soda acid, organic solvent.
Embodiment 1:
Shown in Fig. 1,2, cell climbing sheet 4 is positioned in the creep plate standing groove of creep plate support, the creep plate support is put into the cultivation box body 6 that adapts, add an amount of cell suspension, cover and cultivate lid 5, cell is because action of gravity, at the bottom of can being layered on cell climbing sheet 4 fronts (top) and cultivating box on 7, and adherent growth then.Cell in a plurality of cultivation boxes can apply different processing.After cultivating end, the creep plate support is taken out from cultivate box body 6, place other square to cultivate box, add the liquid-solid cell of deciding on the cell climbing sheet 4 of cell fixation that did not have the cell climbing sheet support, cell fixation discards cell fixation liquid after finishing, and square is cultivated box carry out mark, cryopreservation is handled use for various experiments such as HE dyeing, ICC, ISH, FISH.The cell of 7 growths can collect in method digestion routinely at the bottom of the cultivation box originally, enters experiment flows such as nucleic acid, protein preparation, and is used for various corresponding research methods and index test experience.
Embodiment 2:
With the ICC experiment flow is that example is explained the concrete using method of cell climbing sheet 4.As shown in Figure 4, on common slide glass or creep plate brassboard, drip a droplet glue (water white transparency, stick together firmly, the time of sticking together is short, the corrosive glue of acid and alkali-resistance and organic solvent etc., as UV glue), get the cell climbing sheet 4 of the different treatment group of embodiment 1 preparation, positive (top) adheres on slide glass or the creep plate brassboard up, and filter paper is inhaled and removed unnecessary glue.Cell climbing sheet 4 is integrated into array, routinely the method flow of tissue slice immunohistochemical staining (IHC) to this cell climbing sheet array integrally handle, mounting, observe under the mirror then, take pictures, get final product high quality, high-throughput, the rapid ICC experiment of finishing the cell climbing sheet 4 of a large amount of different treatment easily.
Claims (7)
1, the integrated preparation box of a kind of cell and cell climbing sheet, comprise the cultivation box, cell climbing sheet (4), it is characterized in that, also have as the creep plate support that is positioned in the described cultivation box: the bottom of the bracket base of square frame shape (2) is provided with leg (2a), bracket base (2) equal intervals, many creep plate fixed strips (1) have been arranged in parallel, the narrowed portion of " worker " shaped sections of this fixed strip forms creep plate standing groove (3), and the creep plate standing groove (3) on adjacent two fixed strips is for placing the position of cell climbing sheet (4).
2, according to described cell of claim 1 and the integrated preparation box of cell climbing sheet, it is characterized in that: lath two ends, described creep plate fixed strip (1) " worker " shaped sections top respectively have in 30～60 ° to the oblique angle.
3, according to described cell of claim 2 and the integrated preparation box of cell climbing sheet, it is characterized in that: the dark 0.5mm of the creep plate standing groove (3) on the described creep plate fixed strip (1), wide 0.2mm is matte surface.
4, according to described cell of claim 3 and the integrated preparation box of cell climbing sheet, it is characterized in that: the length of side 4～10cm of described bracket base (2), high 1～2mm has four legs (2a), the high 1～3mm of leg on the wide 2～3mm, four jiaos of bracket bases.
5, according to described cell of claim 4 and the integrated preparation box of cell climbing sheet, it is characterized in that: described cell climbing sheet (4) is square or circular, and thick 0.1～0.2mm, the cell climbing sheet length of side or diameter are 4～8mm.
6, according to described cell of claim 5 and the integrated preparation box of cell climbing sheet, it is characterized in that: cultivate box and be square or circular, and by cultivating box body (6) and cultivating lid (5) and form; Square is cultivated the box body side ratio creep plate bracket lip 6～10mm that grows up, and the circular box body of cultivating can just be put into the creep plate support, cultivates box body bottom thickness 1～2mm, cultivates the length of side of lid or diameter than the big 6～10mm that cultivates box body.
7, according to described cell of the arbitrary claim of claim 1～6 and the integrated preparation box of cell climbing sheet, it is characterized in that: described cultivation box, cell climbing sheet (4) and creep plate support are made by the transparent material of high temperature resistant, acid and alkali-resistance, organic solvent-resistant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200807195U CN201424477Y (en) | 2009-05-08 | 2009-05-08 | Integrated preparation box for cell and cell climbing sheet |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200807195U CN201424477Y (en) | 2009-05-08 | 2009-05-08 | Integrated preparation box for cell and cell climbing sheet |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201424477Y true CN201424477Y (en) | 2010-03-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009200807195U Expired - Fee Related CN201424477Y (en) | 2009-05-08 | 2009-05-08 | Integrated preparation box for cell and cell climbing sheet |
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| CN (1) | CN201424477Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114369536A (en) * | 2022-02-24 | 2022-04-19 | 中南大学湘雅医院 | Cell climbing sheet culture device and application method thereof |
-
2009
- 2009-05-08 CN CN2009200807195U patent/CN201424477Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114369536A (en) * | 2022-02-24 | 2022-04-19 | 中南大学湘雅医院 | Cell climbing sheet culture device and application method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100317 Termination date: 20130508 |