CN202022941U - Culture dish for maturing ovocyte of animal - Google Patents
Culture dish for maturing ovocyte of animal Download PDFInfo
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- CN202022941U CN202022941U CN2010206319499U CN201020631949U CN202022941U CN 202022941 U CN202022941 U CN 202022941U CN 2010206319499 U CN2010206319499 U CN 2010206319499U CN 201020631949 U CN201020631949 U CN 201020631949U CN 202022941 U CN202022941 U CN 202022941U
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- culture dish
- ovocyte
- animal
- concave bottom
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Abstract
A culture dish for maturing an ovocyte of an animal comprises a concave-base dish body and a base matched with the concave-base dish body, wherein a concave base of the concave-base dish body is embedded in the base and is fixed on the base. The culture dish is simple to operate and does not need to be fixed by a hand due to the base supporting the concave-base dish body, and the stability is good; therefore, the culture dish is particularly suitable for the maturing culture of the ovocyte of the animal in a laboratory.
Description
Technical field
The utility model relates to a kind of culture dish, is specifically related to a kind of animal oocyte maturation culture dish.
Background technology
The oocyte in vitro maturation technology provides wide variety of materials for the produced in vitro of animal embryo and relevant embryo engineering technology such as embryo's cutting, freezing preservation, embryonic cell and body-cell neucleus transplanting, transgenosis and sex control etc.
Need after the ovocyte collection to cultivate, could utilize after the maturation through the regular hour.To utilize damping fluid to wash behind the oocyte collection earlier, cultivate with ripe liquid again.Will slough the granulosa cell that adheres on every side with the damping fluid that contains certain enzyme after cultivating maturation, last picking quality ovocyte is preferably used.All these operating process require very strict, holiday cell very easily in washing and picking process especially, and requiring in time simultaneously is manipulation in vitro time weak point as far as possible, and strict aseptic technique.
Present glass culture dish has the matrix culture dish of circular flat bottom culture dish and no base, the former in operating process since at microscopically not under a visual field, need the rotation that hand does not stop, could operate each ovocyte, and to whole bottom be covered with operation liquid, need the amount of operation liquid bigger; Big for the present bore that uses of the latter, no base needs a hand to fix all the time, very easily tilts in operating process, cause ovocyte to run off and pollution, and the ovocyte cultivation needs aseptic technique, and this will cause all ovocytes losses.
Summary of the invention
The purpose of this utility model is to provide a kind of animal oocyte maturation culture dish, and it has solved the technical problem that background technology operation inconvenience, ovocyte are omitted and easily polluted.It makes and operates in the ovocyte culturing process that promptly operation is saved time again and various operation liquid easily, thereby improves the maturing rate of ovocyte.
Technical solution of the present utility model is:
A kind of animal oocyte maturation culture dish comprises a concave bottom ware body, and its special character is: also comprise one with the base of this concave bottom ware body coupling, the concave bottom of described concave bottom ware body is embedded in base, and is fixed on the base.Base support concave bottom ware body is arranged, just do not need hand to fix, good stability.
Above-mentioned base comprises that also one is used to hide the lid of concave bottom ware body.Lid is supporting with the base size, can prevent in the processes such as ovocyte cultivation, observation and photograph not contaminated.
Above-mentioned base is a hollow base.And adopt high-quality crystal polystyrene to make, light transmission is good, is convenient to examine under a microscope.
Above-mentioned base is circular or square.
The utility model is simple to operate, and base support concave bottom ware body is arranged, and does not just need hand to fix, good stability; Increased the lid of the concave bottom ware body that is used to prevent microbial contamination, in incubator, cultivated and examine under a microscope, photograph and moving process middle cover can effectively protect and not be subjected to microbial contamination.
Description of drawings
Fig. 1 is the utility model structural representation;
Fig. 2 is a lid of the present utility model.
Drawing reference numeral explanation: 1-concave bottom ware body, 2-base, 3-concave bottom, 4-lid.
Embodiment
Referring to Fig. 1 and Fig. 2, a kind of animal oocyte maturation culture dish comprises a concave bottom ware body 1, also comprise one with the base 2 of the circular hollow of this concave bottom ware body 1 coupling, the concave bottom of concave bottom ware body 1 is embedded in base 2, and is fixed on the base 2; Base 2 comprises that also one is used to hide the lid 4 of concave bottom ware body 1.
Compared with the existing, have following beneficial technical effects:
The at present various glass dishes that are used for the ovocyte cultivation will thoroughly clean before use, otherwise any material composition all can influence the quality of ovocyte.Also need to carry out sterilization then, could use like this.This utility model culture dish adopts high-quality crystal polystyrene to make, and disposable use has overcome the shortcoming that present glass culture dish is difficult to clean, and needing also to have avoided complex processes such as sterilization, baking and preservation.
The utility model has round base 2, concave surface and be furnished with and cover 4.Do not need a hand fixing separately; the amount of operation liquid can according to how many an amount of interpolations of ovocyte in the operating process; all ovocytes can be operated in a visual field, can effectively protect at cultivation, microscopically observation and moving process middle cover 4 not to be subjected to microbial contamination.
The utility model can be applied to biological experiment research, the outer Embryo Production of animal body and human supplementary reproduction aspect, can shorten the Oocyte in Vitro operating process, alleviates the experiment fees burden and reduces cost, and produces good society, economic benefit.
The utility model is by improving present glass culture dish, a kind of animal oocyte maturation culture dish is provided, the maturation that is used for animal embryo produced in vitro, animal and people's supplementary reproduction process ovocyte is cultivated, easy and simple to handle, anti-pollution, save time, save operation liquid reduces cost.
According to animal ovocyte physilogical characteristics, the concave surface of concave bottom ware body 1 once can ripely be cultivated 1～200 ovocyte.Once can wash, change routine operations such as liquid, quality screening and classification to 500～800 ovocytes.A whole set of culture dish adopts high-quality crystal polystyrene to make, and light transmission is good, is convenient to microscopically and observes, counts and take a picture.
Claims (4)
1. an animal oocyte maturation culture dish comprises a concave bottom ware body, it is characterized in that: also comprise one with the base of this concave bottom ware body coupling, the concave bottom of described concave bottom ware body is embedded in base, and is fixed on the base.
2. according to the described animal oocyte maturation of claim 1 culture dish, it is characterized in that: described base comprises that also one is used to hide the lid of concave bottom ware body.
3. according to claim 1 or 2 described animal oocyte maturation culture dish, it is characterized in that: described base is a hollow base.
4. according to the described animal oocyte maturation of claim 3 culture dish, it is characterized in that: described base is for circular or square.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206319499U CN202022941U (en) | 2010-11-25 | 2010-11-25 | Culture dish for maturing ovocyte of animal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206319499U CN202022941U (en) | 2010-11-25 | 2010-11-25 | Culture dish for maturing ovocyte of animal |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202022941U true CN202022941U (en) | 2011-11-02 |
Family
ID=44847901
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010206319499U Expired - Fee Related CN202022941U (en) | 2010-11-25 | 2010-11-25 | Culture dish for maturing ovocyte of animal |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202022941U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696484A (en) * | 2012-06-20 | 2012-10-03 | 安徽农业大学 | Tissue culture vessel and tissue culture method |
| CN109439527A (en) * | 2018-11-02 | 2019-03-08 | 滕红林 | A kind of microbial cell culture dish |
-
2010
- 2010-11-25 CN CN2010206319499U patent/CN202022941U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696484A (en) * | 2012-06-20 | 2012-10-03 | 安徽农业大学 | Tissue culture vessel and tissue culture method |
| CN109439527A (en) * | 2018-11-02 | 2019-03-08 | 滕红林 | A kind of microbial cell culture dish |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111102 Termination date: 20121125 |