CN204198745U - A kind of multifunctional unit based on micro-fluidic chip is analyzed porous cell and is cultivated chip - Google Patents
A kind of multifunctional unit based on micro-fluidic chip is analyzed porous cell and is cultivated chip Download PDFInfo
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- CN204198745U CN204198745U CN201420543019.6U CN201420543019U CN204198745U CN 204198745 U CN204198745 U CN 204198745U CN 201420543019 U CN201420543019 U CN 201420543019U CN 204198745 U CN204198745 U CN 204198745U
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- cell
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- culture insert
- capsule
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- 238000004113 cell culture Methods 0.000 claims abstract description 70
- 239000002775 capsule Substances 0.000 claims abstract description 17
- 239000011148 porous material Substances 0.000 claims abstract description 15
- 239000004205 dimethyl polysiloxane Substances 0.000 claims abstract description 11
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims abstract description 11
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims abstract description 11
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 8
- 230000002441 reversible effect Effects 0.000 claims abstract description 7
- 239000000126 substance Substances 0.000 claims abstract description 4
- 239000000203 mixture Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 11
- 238000002474 experimental method Methods 0.000 abstract description 7
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 5
- 238000011081 inoculation Methods 0.000 abstract description 5
- 238000003556 assay Methods 0.000 abstract description 4
- 230000000007 visual effect Effects 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 239000007963 capsule composition Substances 0.000 abstract description 2
- 238000012744 immunostaining Methods 0.000 description 5
- 230000010261 cell growth Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 238000003501 co-culture Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000008614 cellular interaction Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of multifunctional unit based on micro-fluidic chip is analyzed porous cell cultivation chip and is belonged to cell culture assays technical field.This cell cultivation chip is primarily of cell culture insert, capsule composition; Cell culture insert is positioned at capsule inner bottom part, forms with the reversible sealing-in of capsule; Cell culture insert there are 2 ~ 6 pore chambers.The material of described cell culture insert is the PDMS polymkeric substance that light-permeable is ventilative, and described pore chamber is made by punch tool.Described cell culture insert is thickness is 1mm ~ 5mm.The utility model cell cultivation chip tool is easy and simple to handle, with low cost, reagent and cell consumption amount low, the microscopic examination visual field affects without obvious light and shade, when liquid feeding changes liquid, cell is not easily rushed, during cell inoculation, the more equal first-class advantage of density, can realize specific experiment purpose, have a wide range of applications in cell cultures and assay.
Description
Technical field
The utility model relates to the technical field of cell culture assays, is specifically related to a kind of multifunctional unit based on micro-fluidic chip and analyzes porous cell cultivation chip.
Background technology
Various types of cells on market cultivates orifice plate and Tissue Culture Dish carries out cell-seeding-density inequality, and cell is easily assembled, and fringe field of view is unclear, just can only be easy to observe at region intermediate.Carry out immunostaining related experiment reagents ratio costly, as more in wasted reagent when 12 orifice plates, 24 orifice plates carry out immunostaining, when liquid feeding changes liquid, bottom cell is easily rushed; And although 96 orifice plates are saved relatively, the visual field is unclear cannot carry out microscopic examination clearly substantially, also easily during liquid feeding is rushed by cell, and if be not once make a large amount of samples, also can cause the waste in unnecessary hole.Usual cell cultivates orifice plate and Tissue Culture Dish also only can carry out conventional cell experiment, cannot observe the stimulation in batches of various kinds of cell interaction or many factors, can not meet experiment demand special as co-culture of cells.
The science and technology that microfluidic chip technology develops rapidly as one, the advantage of its uniqueness has been presented at biomedical sector, more because of its with cell size coupling, environment is close with physiological environment, can provide in Time and place dimension and manipulate more accurately, realize the features such as various kinds of cell functional study easily through flexible design and become the Important Platform of bionic of new generation and cell research.The cell analysis platforms based on micro-fluidic chip in existing stage is that the PDMS silica gel material of utilization with good biocompatibility is as chip material mostly, there is good biocompatibility, light transmission ventilation, realize cell cultures in chip and various biophysics, biological chemistry stimulate to obtain the Behavioral change of cell under different extracellular microenvironment, such as survive, breed, apoptosis, differentiation etc.The character of PDMS chip reversible sealing-in after dehydrated alcohol process, airtight no leakage when ensureing sealing-in, can also at any time experimentally demand take the overall chip of PDMS off.Utilize PDMS material make be applicable to microscopic examination, immunostaining cell culture insert carry out cell analysis and as the particular experiment such as co-culture of cells be also in blank the stage.
Summary of the invention
The purpose of this utility model is to provide a kind of cell cultivation chip based on facture of microchip technology, and this cell cultivation chip is applied to the research of cell in particular experiment such as microscopic examination, immunostaining and co-culture of cells.
A kind of multifunctional unit based on micro-fluidic chip of the utility model is analyzed porous cell and is cultivated chip, and this cell cultivation chip is primarily of cell culture insert, capsule composition; Cell culture insert is positioned at capsule inner bottom part, sealing-in reversible with capsule; Cell culture insert there are 2 ~ 6 pore chambers.
The material of described cell culture insert (1) is the PDMS polymkeric substance that light-permeable is ventilative, and described pore chamber is made by punch tool.
Described cell culture insert is thickness is 1mm ~ 5mm.
Cell culture insert first through soaked in absolute ethyl alcohol 12 hours, 80 DEG C dry after and the reversible sealing-in of capsule
The cell cultivation chip that the utility model provides, because cell culture insert material light transmission is good, and highly lower, examine under a microscope cell cultures district and be subject to the impact of cultivating pool wall less, in the visual field, do not have obvious light and shade to affect.
The cell cultivation chip that the utility model provides, because volume is little, required cell inoculation liquid measure is little, and during cell inoculation, density is more homogeneous, and when liquid feeding changes liquid, the operation of micro updating is very little to impact cell, is not easily rushed by cell.
The cell cultivation chip that the utility model provides, can inoculate different cell, when Growth of Cells is good, adds nutrient solution and do not have cell culture insert in capsule, observe the relative influence of other cells of factor pair of different emiocytosis in the different pore chamber of cell culture insert.
The cell cultivation chip that the utility model provides, different cell can be inoculated in the different pore chamber of cell culture insert, when Growth of Cells is good, cell culture insert can be taken off, whether in capsule, add nutrient solution continue to cultivate, observing different iuntercellular has the effect promoting migration mutually or repel.
Cell cultivation chip of the present utility model is used to carry out the inoculation of cell with when cultivating: cell density is 5 × 10
5cells/mL, liquid volume added is 30 μ L-150 μ L.
The cell cultures based on cell cultivation chip that the utility model provides, immunostaining microscopy observation method, can adopt biologically conventional cell cultures, the cell of detection means to cell cultivation chip cultivate, detect.
The utility model cell cultivation chip tool is easy and simple to handle, with low cost, reagent and cell consumption amount low, the microscopic examination visual field affects without obvious light and shade, when liquid feeding changes liquid, cell is not easily rushed, during cell inoculation, the more equal first-class advantage of density, can realize specific experiment purpose, have a wide range of applications in cell cultures and assay.
Accompanying drawing explanation
Fig. 1 the utility model cell cultivation chip one-piece construction schematic diagram; Wherein 1 is capsule, and 2 is cell culture insert, and 3 is pore chamber.
Embodiment
The following examples will be further described the utility model, but therefore not limit the utility model.
Multifunctional unit based on micro-fluidic chip is analyzed porous cell and is cultivated a chip, and this cell cultivation chip forms primarily of cell culture insert 1, capsule 2; Cell culture insert 1 is positioned at capsule 2 inner bottom part, forms with the reversible sealing-in of capsule 2; Cell culture insert there are 2 ~ 6 pore chambers 3.
The material of described cell culture insert 1 is the PDMS polymkeric substance that light-permeable is ventilative, and described pore chamber 3 is made by punch tool.
Described cell culture insert 1 for thickness be 1mm ~ 5mm.
The cell cultivation chip that the utility model provides, different cell can be inoculated in the different pore chamber of cell culture insert 13, when Growth of Cells is good, in capsule 2, adds nutrient solution do not have cell culture insert 1, observe the relative influence of other cells of factor pair of different emiocytosis.
The cell cultivation chip that the utility model provides, different cell can be inoculated in the different pore chamber of cell culture insert 13, when Growth of Cells is good, cell culture insert 1 can be taken off, whether in capsule 2, add nutrient solution continue to cultivate, observing different iuntercellular has the effect promoting migration mutually or repel.
The establishment method of the utility model cell cultivation chip is:
75mm specification slide is used to carry out enclosing sheet, pouring PDMS monomer: the PDMS of initiator (V:V)=12:1, be highly 2mm, vacuum stripping is steeped, 80 DEG C of gels 1 hour, taking-up cools, solidification PDMS plate is cut into the cell culture insert junior unit of 20mm × 20mm, use punch tool to make the pore chamber that 5 diameters are 6mm thereon, by cell culture insert reversible sealing-in with capsule after soaked in absolute ethyl alcohol is spent the night, uv irradiating spends the night after sterilizing and can use, and structure as shown in Figure 1.
Utilize the cell cultivation chip of laboratory self manufacture, after cell dissociation, being diluted to concentration is 5 × 10
5the cell suspension of cells/mL, 50 μ L add cell culture insert, drip PBS solution increase humidity minimizing reagent and volatilize, move in CO2gas incubator and continue to cultivate around cultivation pool, cell attachment uniform spreading, behind cell culture insert bottom surface, carry out related immune dyeing process.
Claims (4)
1. the multifunctional unit based on micro-fluidic chip analyzes a porous cell cultivation chip, it is characterized in that: this cell cultivation chip is primarily of cell culture insert (1), capsule (2) composition; Cell culture insert (1) is positioned at capsule (2) inner bottom part, forms with capsule (2) reversible sealing-in; Cell culture insert there are 2 ~ 6 pore chambers (3).
2. according to cell cultivation chip according to claim 1, it is characterized in that: the material of described cell culture insert (1) is the PDMS polymkeric substance that light-permeable is ventilative, and described pore chamber (3) is made by punch tool.
3., according to cell cultivation chip according to claim 1, it is characterized in that described cell culture insert (1) thickness is 1mm ~ 5mm.
4., according to cell cultivation chip according to claim 1, it is characterized in that the diameter of described pore chamber (3) is: 5mm ~ 10mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420543019.6U CN204198745U (en) | 2014-09-22 | 2014-09-22 | A kind of multifunctional unit based on micro-fluidic chip is analyzed porous cell and is cultivated chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420543019.6U CN204198745U (en) | 2014-09-22 | 2014-09-22 | A kind of multifunctional unit based on micro-fluidic chip is analyzed porous cell and is cultivated chip |
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| Publication Number | Publication Date |
|---|---|
| CN204198745U true CN204198745U (en) | 2015-03-11 |
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| CN201420543019.6U Expired - Lifetime CN204198745U (en) | 2014-09-22 | 2014-09-22 | A kind of multifunctional unit based on micro-fluidic chip is analyzed porous cell and is cultivated chip |
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| CN (1) | CN204198745U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105907641A (en) * | 2016-05-19 | 2016-08-31 | 大连医科大学附属第医院 | Assembly type multi-condition parallel-culture microfluidic control device and using method thereof |
| CN108148756A (en) * | 2016-12-05 | 2018-06-12 | 中国科学院大连化学物理研究所 | A kind of preparation method of low adherency culture plate |
| CN109055213A (en) * | 2018-08-14 | 2018-12-21 | 深圳职业技术学院 | A kind of production method of simple Micro-CPE neutralization test and the device and the device of counting |
| CN115992050A (en) * | 2023-02-23 | 2023-04-21 | 深圳市第二人民医院(深圳市转化医学研究院) | Controllable rigidity modulus culture dish and preparation method and application thereof |
-
2014
- 2014-09-22 CN CN201420543019.6U patent/CN204198745U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105907641A (en) * | 2016-05-19 | 2016-08-31 | 大连医科大学附属第医院 | Assembly type multi-condition parallel-culture microfluidic control device and using method thereof |
| CN108148756A (en) * | 2016-12-05 | 2018-06-12 | 中国科学院大连化学物理研究所 | A kind of preparation method of low adherency culture plate |
| CN109055213A (en) * | 2018-08-14 | 2018-12-21 | 深圳职业技术学院 | A kind of production method of simple Micro-CPE neutralization test and the device and the device of counting |
| CN115992050A (en) * | 2023-02-23 | 2023-04-21 | 深圳市第二人民医院(深圳市转化医学研究院) | Controllable rigidity modulus culture dish and preparation method and application thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20150311 |