CN207895144U - For microscopical load sample device - Google Patents
For microscopical load sample device Download PDFInfo
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- CN207895144U CN207895144U CN201820229410.7U CN201820229410U CN207895144U CN 207895144 U CN207895144 U CN 207895144U CN 201820229410 U CN201820229410 U CN 201820229410U CN 207895144 U CN207895144 U CN 207895144U
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- hole
- upper plate
- coverslip
- load sample
- sample device
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Abstract
One kind being used for microscopical load sample device, and the load sample device includes：Upper plate, washer, coverslip, lower plate and glue lattice, it is characterised in that：The center of the upper plate has a through-hole, the size of through-hole to be less than the coverslip；The washer is fabricated from a flexible material, and center has a through-hole, the size of through-hole consistent with the through-hole of the upper plate；The coverslip is used for carrying sample；The shapes and sizes of the lower plate are identical as the upper plate, there is a through-hole in center, and the size of through-hole is consistent with the through-hole of the upper plate, the upper plate and the lower plate are equipped with coupling device, for combining the load sample device, the glue lattice are sticked to by adhesive on the coverslip, and coverslip is separated into independent cell, the glue lattice are fabricated from a flexible material, and there is a through-hole in center.The present apparatus can be used for high power objective observation, increase the range of high power lens microexamination.The present apparatus can Reusability, it is at low cost, and be widely used.
Description
Technical field
The utility model is that one kind being suitable for microscopical load sample device, is particularly suitable for the observation of high power objective.
Background technology
The load sample device of current micro- sem observation has culture dish (Fig. 1), orifice plate, culture bottle, glass slide etc., but these are filled
It sets because bottom is thicker, is more than high power objective operating distance, therefore cannot be used for high power objective observation.If wanting to be observed with high power objective
There are two types of methods：A kind of method is that coverslip is added in cell culture fluid, and cell paving is allowed then to salvage lid on the cover slip
Slide carries out the operations such as follow-up fluorescent staining, and last coverslip is contacted with object lens carries out microexamination.But coverslip and ware bottom
Fitting is closer, it is difficult to salvage, and carries out being easy coverslip doing anti-when follow-up sample preparation operation, fluorescent dye is made to add wrong face,
Dyeing is caused to fail.
Another method is to use glass bottom ware (Fig. 2), has a circular hole among this culture dish, is stained with coverslip below.Carefully
Born of the same parents cultivate in the ware of glass bottom, sample preparation, finally directly with micro- sem observation.But the range of observation is confined in this circular hole.And
Glass bottom ware is medical disposable material, cannot be reused, cost is very high.
It is about 1cm that two kinds of load sample devices in Fig. 1 and Fig. 2, which have side wall, height, the so high side wall in micromanipulation
The angle of inclination of serious limitation microneedle and moving range.
Utility model content
In order to solve the defects of load sample device base thickness degree is big, range of observation is small, of high cost in the prior art, the utility model
It proposes a kind of for microscopical load sample device.
The utility model provides one kind and being used for microscopical load sample device, including：Upper plate, washer, coverslip, lower plate with
And glue lattice, it is characterised in that：The center of the upper plate has a through-hole, through-hole to be dimensioned slightly smaller than the coverslip；The washer
It is fabricated from a flexible material, and center has a through-hole, the size of through-hole consistent with the through-hole of the upper plate；The coverslip is used for
Carry sample；The shapes and sizes of the lower plate are identical as the upper plate, and there is a through-hole in center, and the size of through-hole with it is described
The through-hole of upper plate is consistent, and the upper plate and the lower plate are equipped with coupling device, for combining the load sample device, institute
It states glue lattice to be sticked on the coverslip by adhesive, coverslip is separated into independent cell, the glue lattice are by flexible material
It is made, and there is a through-hole in center.
Preferably, the upper plate and lower plate are made of rigid material respectively, the rigid material be selected from plastics, stainless steel or
Aluminium alloy.
Preferably, there are scale or number around the through-hole of the upper plate.
Preferably, the shape of the glue lattice be square, rectangle or circle, it is alternatively possible to use multiple glue lattice will
One coverslip is separated into multiple cells, to load different samples.
Preferably, the flexible material of the washer and glue lattice is respectively selected from silica gel or rubber.
Preferably, the coupling device is locker.
Preferably, the upper plate is equipped with lock, and the lower plate is equipped with locked groove or the upper plate is equipped with locked groove, under described
Plate is equipped with lock.
Compared with prior art, the load sample device of the utility model has the advantages that：
1, can make repeatedly after utility model device uses material (including upper plate, lower plate, washer, glue lattice) is sterilized
With only coverslip is consumables, has saved material, cost is greatly reduced compared with disposable glass bottom ware.
2, utility model device is load sample on the cover slip, the coverslip that thickness is less than 0.17mm can be selected, so can
It is observed with high power objective, such as 100 times of oil mirrors.The load sample device upper plate, lower plate, the size of washer through-hole are about that coverslip is big
It is small, even if so observed with high power objective, it can also be observed that the field range of about entire coverslip.
3, it can be used for cell culture.The utility model is used directly for culture cell since leakproofness is preferable.This practicality
The height of new device can be designed to 1cm, highly consistent with Nostoc commune Vanch ware, cell can load on coverslip, coated with
Cell culture fluid is placed in incubator and cultivates.A coverslip is divided into multiple cells by glue lattice simultaneously, can load multiple samples
Product or same sample carry out a variety of processing, ensure that incubation conditions are consistent, facilitate and carry out single-factor variable analysis.One coverslip
The upper multiple samples of load, are greatly saved the time varied, focused when observation.
4, it can be used for fluorescent staining.Cell can directly carry out the behaviour such as fluorescent staining after being cultivated in utility model device
Make, avoid the operation for salvaging coverslip, while avoiding and coverslip is done anti-, fluorescent dye is made to add wrong face, dyeing is caused to lose
It loses.If using glue lattice, glue lattice size is small, reduces dyeing range, to save antibody and fluorescent dye, reduces experimental cost.
Fluorescent dyeing is carried out using utility model device, in addition buffer solution can be observed directly, is omitted and seals up piece liquid, smears
The step of nail polish, has saved the time of sample preparation.Nail polish is avoided simultaneously and penetrates into sample, and sample is polluted, influences to see
It examines.
5, it can be used for micromanipulation.Utility model device reduces the height of load sample device, and the height of device can be set
0.2-0.5cm is counted into, is common load sample device height 1/5th to half, to increase the inclination of microneedle
Angle and moving range.
6, high stainless steel, the aluminium alloy etc. of density may be selected in upper and lower plate material, increases the weight of load sample device.Load sample fills
Weight increase is set, the visual field is not easy to drift about.In this microexamination under inverted microscope high power objective, especially multiple spot is seen repeatedly
Advantage is more prominent when examining.
Description of the drawings
Fig. 1 is ordinary cells culture dish in the prior art；
Fig. 2 is glass bottom in the prior art ware；
Fig. 3 is the load sample schematic device in the utility model embodiment.
Reference sign：
1- upper plates；2- washers；3- coverslips；4- lower plates；5- glue lattice；6- locked grooves；7- is latched.
Specific implementation mode
To make the purpose of this utility model, technical solution and advantage be more clearly understood, below in conjunction with specific embodiment, and
With reference to attached drawing, the utility model is described in further detail.
The utility model includes for microscopical load sample device：Upper plate 1, washer 2, coverslip 3, lower plate 4 and glue
Lattice 5 are provided with locked groove 6 on upper plate 1, and lock 7 is provided in lower plate 4, and load sample device is whole to be combined by locked groove 6 and lock 7 are whole
Together.
Upper plate 1 can be the arbitrary shapes such as rectangle, square, circle, and there is a through-hole in center.Around through-hole also
Can there are scale or number, precise positioning viewing area is used for, to find the region of previous observation.On in one embodiment
The size of plate 1 is 7.5 × 2.5cm, and thickness about 0.2cm, there are a square hole, the 4 × 2cm of size in hole, hole Zhou Keyou number in center.
Washer 2 is fabricated from a flexible material, and plays the role of closed, can increase the leakproofness of device.The center of washer 2 has
The size of one through-hole, through-hole is identical as the through-hole of upper plate 1.In one embodiment, the size of washer 2 is 7.5 × 2.5cm, thick
About 0.1cm is spent, there are a square hole, the 4 × 2cm of size in hole in center.The size of washer 2 is also smaller than the size of upper plate 1.
Coverslip 3 is used for carrying sample.In one embodiment, 4.5 × 2.5cm of size of coverslip 3.
The shape of lower plate 4 is identical as the upper plate as size, and there is a through-hole in center, the size of through-hole, position and upper plate 1
Through-hole is consistent.
Upper plate 1 is connected with lower plate 4 by locker or other coupling devices, and in another embodiment, the setting of locked groove 6 exists
In lower plate 4, lock 7 is arranged on upper plate 1.Upper plate 1 and lower plate 4 are made of rigid material, the rigid material can be selected from plastics,
Stainless steel, aluminium alloy etc..
The through-hole in upper plate 1 and lower plate 4 and 2 center of washer can design different specifications according to 3 size of coverslip, be slightly less than lid
Slide 3.
Glue lattice 5 are fabricated from a flexible material, and center has a through-hole, glue lattice 5 that can be sticked to coverslip 3 by adhesive
On, coverslip 3 is separated into independent cell.Preferably, coverslip 3 can be separated into using multiple glue lattice 5 multiple small
Room, to load different samples.The shape of glue lattice 5 can be square, rectangle or circle etc., and can be used alone also can be more
It is a to be used together.In one embodiment, the size of glue lattice 5 is 0.5 × 0.5cm.Scale around the through-hole of upper plate 1 or number
Spacing it is consistent with the size of glue lattice 5, play the role of mark glue case set.The material of washer 2 and glue lattice 5 is unlimited, for flexibility
Material, such as silica gel, rubber.
Reusability after upper plate 1, washer 2, lower plate 4 and glue lattice 5 can sterilize, it is cost-effective.
The load sample device of the utility model is suitable for the observation of simple microscope, laser scanning co-focusing microscope etc..Sample
Product are through the sample preparations step such as fluorescent staining, in addition buffer solution can be observed directly, the step for being omitted and sealing up piece liquid, smear nail polish
Suddenly, the time of sample preparation has been saved.And avoid nail polish and penetrate into sample, sample is polluted, influences to observe.
Utility model device also can be directly used for the operations such as fluorescent staining, avoid the operation for salvaging coverslip, simultaneously
It avoids and coverslip is done anti-, fluorescent dye is made to add wrong face, dyeing is caused to fail.If using glue lattice, glue lattice size is small, reduces
Range is dyed, to save antibody and fluorescent dye, reduces experimental cost.Fluorescent dye is carried out using utility model device
Color is omitted in addition buffer solution can be observed directly and seals up piece liquid, smears the step of nail polish, saved the time of sample preparation.Together
When avoid nail polish penetrate into sample, sample is polluted, influences to observe.
The load sample device of the utility model is suitable for micromanipulation, reduces the thickness of load sample device, increases micromanipulation
The angle of inclination of needle and moving range.
The load sample device of the utility model is also suitable for the observation of suspension cell.It can be coated with lid glass with poly-D-lysine
Piece, is then added dropwise suspension cell, then with micro- sem observation.
The load sample device of the utility model can also be used for cell culture.Due to good airproof performance, can be trained with the utility model
Cell is supported, is placed in incubator and cultivates cell.Compared to Nostoc commune Vanch ware, glue lattice size is small, to reduce cell growth
Space greatlys save the reagents such as culture medium, growth factor, reduces experimental cost.Meanwhile glue lattice separate different samples, one
Multiple samples can be handled on coverslip simultaneously, ensure that the incubation conditions of different sample rooms are consistent, it is ensured that monotropic quantity research.
The load sample device of the utility model in addition to coverslip, rest part can high-temperature sterilization, Reusability substantially reduces
Buy the cost of culture dish.Position of the coverslip as carrying sample simultaneously, it is disposable, it avoids not washing clean clearly and causes dirt
Dye.Not only can be to avoid pollution using the utility model, but also reduce cost.
Particular embodiments described above has carried out into one the purpose of this utility model, technical solution and advantageous effect
Step is described in detail, it should be understood that the foregoing is merely specific embodiment of the utility model, are not limited to this reality
With novel, within the spirit and principle of the utility model, any modification, equivalent substitution, improvement and etc. done should all include
It is within the protection scope of the utility model.
Claims (9)
1. one kind being used for microscopical load sample device, including：Upper plate, washer, coverslip, lower plate and glue lattice, it is characterised in that：
The center of the upper plate has a through-hole, the size of through-hole to be less than the coverslip；The washer is fabricated from a flexible material, and in
Centre has a through-hole, the size of through-hole consistent with the through-hole of the upper plate；The coverslip is used for carrying sample；The shape of the lower plate
Shape and size are identical as the upper plate, and there is a through-hole in center, and the size of through-hole is consistent with the through-hole of the upper plate, it is described on
Plate and the lower plate are equipped with coupling device, and for combining the load sample device, the glue lattice are sticked to by adhesive
On the coverslip, coverslip is separated into independent cell, the glue lattice are fabricated from a flexible material, and center has one to lead to
Hole.
2. load sample device as described in claim 1, which is characterized in that the upper plate and lower plate are made of rigid material respectively.
3. load sample device as claimed in claim 2, which is characterized in that the rigid material is selected from plastics, stainless steel or aluminium and closes
Gold.
4. load sample device as described in claim 1, which is characterized in that have scale or number around the through-hole of the upper plate.
5. load sample device as described in claim 1, which is characterized in that the shape of the glue lattice is square, rectangle or circle
Shape.
6. load sample device as described in claim 1, which is characterized in that be separated into a coverslip using multiple glue lattice multiple
Cell, to load different samples.
7. load sample device as described in claim 1, which is characterized in that the flexible material of the washer and glue lattice is respectively selected from silicon
Glue or rubber.
8. load sample device as described in claim 1, which is characterized in that the coupling device is locker.
9. load sample device as claimed in claim 8, which is characterized in that the upper plate is provided with lock, and the lower plate is provided with
Locked groove or the upper plate are provided with locked groove, and the lower plate is provided with lock.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820229410.7U CN207895144U (en) | 2018-02-08 | 2018-02-08 | For microscopical load sample device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820229410.7U CN207895144U (en) | 2018-02-08 | 2018-02-08 | For microscopical load sample device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207895144U true CN207895144U (en) | 2018-09-21 |
Family
ID=63544420
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| Application Number | Title | Priority Date | Filing Date |
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| CN201820229410.7U Active CN207895144U (en) | 2018-02-08 | 2018-02-08 | For microscopical load sample device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110133837A (en) * | 2018-02-08 | 2019-08-16 | 中国科学院微生物研究所 | For microscopical load sample device and its application |
| CN110673324A (en) * | 2019-09-27 | 2020-01-10 | 佛山科学技术学院 | Glass slide structure suitable for confocal microscope |
-
2018
- 2018-02-08 CN CN201820229410.7U patent/CN207895144U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110133837A (en) * | 2018-02-08 | 2019-08-16 | 中国科学院微生物研究所 | For microscopical load sample device and its application |
| CN110673324A (en) * | 2019-09-27 | 2020-01-10 | 佛山科学技术学院 | Glass slide structure suitable for confocal microscope |
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