CN207793288U - A kind of cell culture apparatus taken pictures for laser confocal microscope - Google Patents
A kind of cell culture apparatus taken pictures for laser confocal microscope Download PDFInfo
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- CN207793288U CN207793288U CN201820009799.4U CN201820009799U CN207793288U CN 207793288 U CN207793288 U CN 207793288U CN 201820009799 U CN201820009799 U CN 201820009799U CN 207793288 U CN207793288 U CN 207793288U
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- cell
- glass slide
- frame structure
- baffle
- culture apparatus
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of cell culture apparatus taken pictures for laser confocal microscope, belongs to Bioexperiment clothing arts.The cell culture apparatus includes mainboard, card slot, glass slide and culture cell baffle, the mainboard is the frame structure for including at least one frame, card slot is equipped in the frame structure middle inside and mainboard in the middle part of side frame corresponding with frame structure, the glass slide is placed in inside frame structure, and the edge of glass slide is located in card slot, culture cell baffle is set on glass slide, on the inside of frame structure, and the glass slide upper space in frame structure is divided at least one cell by the culture cell baffle.The utility model is designed reasonably, easy to operation, borate glass slide transparency is high, refractivity is good, convenient for confocal microscopy, takes pictures.Glass slide lower part is hanging, can prevent cell contamination, and prevents the spot interference experiment of glass slide bottom.Using multiple culture compact designs, it is convenient for experiment packet, substantially reduces experimental cost.
Description
Technical field
The utility model belongs to Bioexperiment equipment technical field, and in particular to one kind is clapped for laser confocal microscope
According to cell culture apparatus.
Background technology
Laser scanning confocal microscopy is widely used in field of biomedical research, is commonly used in laser co-focusing on the market
The culture dish of experiment is mostly culture vessel with glass bottom, typically in the plastic culture dish bottom holes Da Ge, then by thickness be 170 ~ 220 μ
The ultra-thin coverslip or cell climbing sheet of m sticks at culture dish bottom with shadowless glue water or silica gel.Glass bottom culture dish bottom surface is thin, translucency
Can be good, laser co-focusing microscope experiment is removed, the requirement of the experiments such as fluorescence microscopy experiment and difference microscope experiment can be also met.But
There are shortcomings：（1）The bottom for not being entire culture dish is all glass material, and the region for being suitable for observation is only being beaten
The region in hole.（2）Due to the hydrophobicity of glass, often when cultivating cell, cell cannot be adherent well on glass,
Growth conditions are simultaneously bad, or even there is a phenomenon where cell flakes when doing confocal scanning imaging.（3）Single culture dish is logical
Measure very little, if one it is secondary make multiple samples, operation will be cumbersome, and reagent dosage can be larger.（4）Culture dish dismounting is tired
Difficulty is unfavorable for subsequent experimental and long-term preservation.（5）Fluorescence can influence each other between hole.（6）The unqualified culture vessel with glass bottom of quality
It is possible that can be because glue have the glue for gluing even and leakage or using toxic to cell.
Utility model content
The technical issues of solution：In view of the above technical problems, the utility model provides a kind of micro- for laser co-focusing
The cell culture apparatus that mirror is taken pictures, have at low cost, operability it is strong, convenient for testing and observe, be conducive to subsequent experimental and length
The advantages that phase preserves.
Technical solution：A kind of cell culture apparatus taken pictures for laser confocal microscope, the cell culture apparatus
Including mainboard, card slot, glass slide and culture cell baffle, the mainboard is the frame structure for including at least one frame, the frame
Card slot is equipped in frame structure middle inside and mainboard in the middle part of side frame corresponding with frame structure, the glass slide is placed in frame
Inside configuration, and the edge of glass slide is located in card slot, and culture cell baffle is set on glass slide, in frame structure
Glass slide upper space in frame structure is divided at least one cell by side, the culture cell baffle.
Preferably, the mainboard is the frame structure for including 3,6 or 12 frames.
Preferably, the glass slide upper space in frame structure is divided into 2 cells by the culture cell baffle.
Preferably, the cell culture apparatus further includes liquid feeding slot, the liquid feeding slot is set to culture cell baffle and surrounds
Cell side, and less than culture cell baffle.
Preferably, the board edges are equipped with screw thread.
Preferably, the mainboard is fillet far from the one side edge equipped with card slot.
Preferably, the glass slide is borate glass slide.
Preferably, culture cell baffle is hot melt adhesive baffle.
Preferably, the cell culture apparatus further includes coverslip, the coverslip is placed on corresponding glass slide top
It cultivates on cell baffle.
Advantageous effect：（1）The utility model is designed reasonably, easy to operate, borate glass slide transparency height, folding
Photosensitiveness is good, convenient for confocal microscopy, takes pictures.（2）The design of the utility model liquid feeding slot can prevent cell contamination with
And because pipette tips blow and beat caused by cellular damage, fall off.（3）The utility model uses hot melt adhesive baffle, can be removed with tweezers, carries glass
Piece can be freely disassembled, and be convenient for subsequent experimental and preservation.（4）The utility model glass slide lower part is hanging, can prevent cell contamination, and
Prevent the spot of glass slide bottom from interfering the burnt observation of copolymerization, taking pictures.（5）The utility model culture apparatus mainboard periphery is equipped with screw thread
Person easy to operation grasps, and the mainboard and hot melt adhesive baffle are black, glimmering between hole when Laser Scanning Confocal Microscope being avoided to take pictures
The influence of light.（6）The utility model mainboard is fillet far from the one side edge set on card slot, prevents coverslip lid anti-.（7）This reality
With novel multiple culture compact designs, it is convenient for experiment packet, it is few each to cultivate cell, reagent needed for cell in experimentation, greatly
It is big to reduce experimental cost.
Description of the drawings
Fig. 1 is the utility model structure diagram；
Fig. 2 is the utility model mainboard structure front view；
Fig. 3 is the utility model mainboard structure vertical view.
Each label represents as follows in figure：1. mainboard；2. card slot；3. glass slide；4. cultivating cell baffle；5. liquid feeding slot.
Specific implementation mode
The utility model is further described in the following with reference to the drawings and specific embodiments.
Embodiment 1
A kind of cell culture apparatus taken pictures for laser confocal microscope, referring to Fig.1, the cell culture apparatus packet
Mainboard 1, card slot 2, glass slide 3 and culture cell baffle 4 are included, with reference to Fig. 2 and Fig. 3, the mainboard 1 is the frame for including 3 frames
Structure is equipped with card slot 2 in the frame structure middle inside and mainboard 1 in the middle part of side frame corresponding with frame structure.It is described
Glass slide 3 is borate glass slide, and the glass slide 3 is placed in inside frame structure, and the edge of glass slide 3 is located at card slot 2
It is interior.Glass slide can be freely disassembled, and be convenient for subsequent operation and long-term preservation.Culture cell baffle 4 be set to glass slide 3 on,
On the inside of frame structure, 3 upper space of glass slide in frame structure is divided into 2 cells by the culture cell baffle 4.It is described thin
Born of the same parents' culture apparatus further includes liquid feeding slot 5, and the liquid feeding slot 5 is set to the cell side that culture cell baffle 4 surrounds, and less than training
Cell baffle 4 is supported, liquid feeding slot is used for liquid feeding, and buffering liquid is to the impact force of cell, to cell when preventing that fluid nutrient medium is added
Physical damnification, prevent cell detachment.
Embodiment 2
A kind of cell culture apparatus taken pictures for laser confocal microscope, the cell culture apparatus include mainboard 1,
Card slot 2, glass slide 3 and culture cell baffle 4, the mainboard 1 is the frame structure for including 6 frames, on the inside of the frame structure
Card slot 2 is equipped in middle part and mainboard 1 in the middle part of side frame corresponding with frame structure, 1 edge of the mainboard is equipped with screw thread, is convenient for
It grasps.The glass slide 3 is borate glass slide, and the glass slide 3 is placed in inside frame structure, and the edge of glass slide 3
In card slot 2.Glass slide can be freely disassembled, and be convenient for subsequent operation and long-term preservation.Culture cell baffle 4, which is set to, to be carried
On slide 3, on the inside of frame structure, 3 upper space of glass slide in frame structure is divided into 2 small by the culture cell baffle 4
Room.The cell culture apparatus further includes liquid feeding slot 5, and the liquid feeding slot 5 is set to the cell side that culture cell baffle 4 surrounds,
And less than culture cell baffle 4, liquid feeding slot is used for liquid feeding, and buffering liquid to the impact force of cell prevents from that Liquid Culture is added
To the physical damnification of cell when base, cell detachment is prevented.
Embodiment 3
A kind of cell culture apparatus taken pictures for laser confocal microscope, the cell culture apparatus include mainboard 1,
Card slot 2, glass slide 3 and culture cell baffle 4, the mainboard 1 is the frame structure for including 12 frames, on the inside of the frame structure
Card slot 2 is equipped in middle part and mainboard 1 in the middle part of side frame corresponding with frame structure, 1 edge of the mainboard is equipped with screw thread, is convenient for
It grasps.The glass slide 3 is borate glass slide, and the glass slide 3 is placed in inside frame structure, and the edge of glass slide 3
In card slot 2.Glass slide can be freely disassembled, and be convenient for subsequent operation and long-term preservation.Culture cell baffle 4 is hot melt
Glue baffle can be removed with tweezers.Culture cell baffle 4 is set on glass slide 3, on the inside of frame structure, the culture cell
3 upper space of glass slide in frame structure is divided into 2 cells by baffle 4.The cell culture apparatus further includes liquid feeding slot 5,
The liquid feeding slot 5 is set on the cell side that culture cell baffle 4 surrounds, and less than culture cell baffle 4, liquid feeding slot is for adding
Liquid, buffering liquid to the physical damnification of cell when preventing from being added fluid nutrient medium, prevent cell detachment to the impact force of cell.
The cell culture apparatus further includes coverslip, and the coverslip is placed on the culture cell on corresponding glass slide top 3
On baffle 4.The main body 1 is fillet far from the one side edge equipped with card slot 2, prevents coverslip lid anti-.And mainboard 1 and culture
Cell baffle 4 is black, when Laser Scanning Confocal Microscope being avoided to take pictures between hole fluorescence influence.
The utility model is designed reasonably, easy to operate, borate glass slide transparency is high, refractivity is good, convenient for altogether
Focusing microscope observation is taken pictures, and the utility model glass slide lower part is hanging, can prevent cell contamination, and prevent glass slide
The burnt observation of spot interference copolymerization of bottom is taken pictures.It is every in experimentation convenient for experiment packet using multiple culture compact designs
Cell, reagent needed for a culture cell are few, substantially reduce experimental cost.
Claims (9)
1. a kind of cell culture apparatus taken pictures for laser confocal microscope, which is characterized in that the cell culture apparatus
Including mainboard（1）, card slot（2）, glass slide（3）With culture cell baffle（4）, the mainboard（1）It includes at least one frame to be
Frame structure is equipped with card slot in the frame structure middle inside and mainboard in the middle part of side frame corresponding with frame structure（2）,
The glass slide（3）It is placed in inside frame structure, and glass slide（3）Edge be located at card slot（2）It is interior, the culture cell gear
Plate（4）Set on glass slide（3）On the inside of upper, frame structure, culture cell baffle（4）By the glass slide in frame structure（3）
Upper space is divided at least one cell.
2. a kind of cell culture apparatus taken pictures for laser confocal microscope according to claim 1, feature exist
In the mainboard（1）It is the frame structure for including 3,6 or 12 frames.
3. a kind of cell culture apparatus taken pictures for laser confocal microscope according to claim 1, feature exist
In culture cell baffle（4）By the glass slide in frame structure（3）Upper space is divided into 2 cells.
4. a kind of cell culture apparatus taken pictures for laser confocal microscope according to claim 1, feature exist
In the cell culture apparatus further includes liquid feeding slot（5）, the liquid feeding slot（5）Set on culture cell baffle（4）The cell surrounded
Side, and less than culture cell baffle（4）.
5. a kind of cell culture apparatus taken pictures for laser confocal microscope according to claim 1, feature exist
In the mainboard（1）Edge is equipped with screw thread.
6. a kind of cell culture apparatus taken pictures for laser confocal microscope according to claim 1, feature exist
In the mainboard（1）Far from equipped with card slot（2）One side edge be fillet.
7. a kind of cell culture apparatus taken pictures for laser confocal microscope according to claim 1, feature exist
In the glass slide（3）For borate glass slide.
8. a kind of cell culture apparatus taken pictures for laser confocal microscope according to claim 1, feature exist
In culture cell baffle（4）For hot melt adhesive baffle.
9. a kind of cell culture apparatus taken pictures for laser confocal microscope according to claim 1, feature exist
In the cell culture apparatus further includes coverslip, and the coverslip is placed on corresponding glass slide top（3）Culture cell baffle
（4）On.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820009799.4U CN207793288U (en) | 2018-01-04 | 2018-01-04 | A kind of cell culture apparatus taken pictures for laser confocal microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820009799.4U CN207793288U (en) | 2018-01-04 | 2018-01-04 | A kind of cell culture apparatus taken pictures for laser confocal microscope |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207793288U true CN207793288U (en) | 2018-08-31 |
Family
ID=63277157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820009799.4U Expired - Fee Related CN207793288U (en) | 2018-01-04 | 2018-01-04 | A kind of cell culture apparatus taken pictures for laser confocal microscope |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207793288U (en) |
-
2018
- 2018-01-04 CN CN201820009799.4U patent/CN207793288U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180831 Termination date: 20210104 |