CN218879935U

CN218879935U - Cell culture container

Info

Publication number: CN218879935U
Application number: CN202320064577.3U
Authority: CN
Inventors: 陈怀勇; 陈熹; 熊英
Original assignee: Wuhan Beisuo Cell Science And Technology Research Institute Co ltd
Current assignee: Wuhan Beisuo Cell Science And Technology Research Institute Co ltd
Priority date: 2023-01-09
Filing date: 2023-01-09
Publication date: 2023-04-18
Anticipated expiration: 2033-01-09

Abstract

The utility model relates to a cell culture container technical field just discloses a cell culture container, including constant temperature base subassembly, the fixedly connected with culture dish base of axle center department of constant temperature base subassembly upper surface, the last fixed surface of culture dish base is connected with cross baffle, spacing screw hole has been seted up in the axle center department of cross baffle, open the inside of cross baffle and all be equipped with spacing spout, the inside sliding connection of spacing spout has cell culture dish subassembly, there is the closing cap on the top of cross baffle through screw bolt threaded connection. This cell culture container through the setting of constant temperature base subassembly, constant temperature lid subassembly, can provide a homothermal place for cell culture, promotes the cell reproduction, improves the survival rate, can completely cut off external bacterium and also played dirt-proof effect, through the setting of spacing spout, cell culture dish subassembly, the access cell of being convenient for is equipped with a plurality of culture tanks, and the increase contrast can accelerate culture efficiency.

Description

Cell culture container

Technical Field

The utility model relates to a cell culture container technical field specifically is a cell culture container.

Background

Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. A large number of cells or their metabolites are obtained by cell culture. Since biological products are all derived from cells, it can be said that cell culture technology is the most core and basic technology in biotechnology. Since the culture conditions of cells are special, a cell culture vessel is required for carrying.

One of the existing cell culture vessels has the following disadvantages:

(1) The cell culture container is single, the contrast is low, and different types of cells cannot be put together for simultaneous propagation.

(2) The tightness is poor, the cell propagation is easily influenced by bacteria, and cell culture technicians are required to register on the notebook, so that real-time visual observation is hindered.

Therefore, the utility model provides a cell culture container.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

Not enough to prior art, the utility model provides a cell culture container has solved the influence of the difference in temperature that receives that proposes in the above-mentioned background art, and cell culture container is single, and the seal is poor, needs the problem of cell culture technical staff registration on this son.

(II) technical scheme

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: the utility model provides a cell culture container, includes constant temperature base subassembly, the fixedly connected with culture dish base of axle center department of constant temperature base subassembly upper surface, the last fixed surface of culture dish base is connected with cross baffle, spacing screw hole has been seted up to cross baffle's axle center department, open the inside of cross baffle all is equipped with spacing spout, the inside sliding connection of spacing spout has cell culture dish subassembly, the top of cross baffle has airtight lid through the threaded bolt threaded connection, constant temperature cover subassembly has been cup jointed to the outside of constant temperature base subassembly, the fixed surface of cell culture dish subassembly is connected with the record board.

Optionally, the constant temperature base assembly comprises a temperature control base, a clamping groove is formed in the periphery of the upper surface of the temperature control base, an anti-slip mat is fixedly connected to the bottom of the temperature control base, and a sealing ring is sleeved inside the clamping groove.

Optionally, the cell culture dish subassembly includes the cell culture dish, the surperficial threaded connection of cell culture dish has the handle, the spacing slider of bottom fixedly connected with of cell culture dish, spacing slider sliding connection is in the inside of spacing spout, the quantity of cell culture dish is four.

Optionally, the constant temperature cover assembly comprises a constant temperature cover, a circle center of the upper surface of the constant temperature cover is fixedly connected with a cover button, and the constant temperature cover is clamped with the upper end of the constant temperature base assembly.

Optionally, the material of the closed cover is transparent glass, and the bottom end of the closed cover is clamped in the constant temperature base assembly.

Optionally, the inside fixedly connected with spacing slider of spacing spout, the material of spacing slider is the rubber material.

(III) advantageous effects

The utility model provides a cell culture container possesses following beneficial effect:

1. this cell culture container through the setting of constant temperature base subassembly, constant temperature lid subassembly, can provide a homothermal place for cell culture, promotes the cell reproduction, improves the survival rate, can completely cut off external bacterium and also played dirt-proof effect, through the setting of spacing spout, cell culture dish subassembly, the access cell of being convenient for is equipped with a plurality of culture tanks, and the increase contrast can accelerate culture efficiency.

2. This cell culture container, through the setting of culture dish base, cross baffle, airtight lid, screw bolt, the cell interactive reaction in the isolated different culture tanks makes data safe and reliable more, through the setting of record board, can register the cell reproduction condition of different time quantums, makes things convenient for cell culture technical staff to make statistics of cell culture data.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the structure of the culture dish base, the cross-shaped partition plate, the limiting chute, the cell culture dish assembly, the sealing cover and the threaded bolt of the present invention;

FIG. 3 is a schematic view of the structure of the constant temperature base assembly of the present invention;

fig. 4 is a schematic cross-sectional structure diagram of the present invention.

In the figure: 1. a constant temperature base assembly; 101. a temperature control base; 102. a card slot; 103. a non-slip mat; 2. a culture dish base; 3. a cross-shaped partition plate; 4. a limiting chute; 5. a cell culture dish assembly; 501. a cell culture dish; 502. a handle; 503. a limiting slide block; 6. a threaded bolt; 7. a hermetic cover; 8. a constant temperature cap assembly; 801. a constant temperature cover; 802. a cover button; 9. and (5) recording the plate.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1 to 4, the present invention provides a technical solution: a cell culture container comprises a constant temperature base component 1, a constant temperature condition is provided for cell culture through the arrangement of the constant temperature base component 1, the cell propagation efficiency is increased, a culture dish base 2 is fixedly connected with the axis position of the upper surface of the constant temperature base component 1, the effect of supporting a cross-shaped partition plate 3 is achieved through the arrangement of the culture dish base 2, the cross-shaped partition plate 3 is fixedly connected with the upper surface of the culture dish base 2, the effect of isolating cell interaction in a cell culture dish component 5 is achieved through the arrangement of the cross-shaped partition plate 3, a limit threaded hole is formed in the axis position of the cross-shaped partition plate 3, the effect of facilitating the threaded fixation of a sealing cover 7 through a threaded bolt 6 is achieved through the arrangement of the threaded hole, limit sliding grooves 4 are formed in the inner part of the cross-shaped partition plate 3, and through the arrangement of the limit sliding grooves 4, the cell culture dish assembly has the advantages that the cell culture dish 501 is driven by the limiting sliding block 503 to move on the culture dish base 2 conveniently, the cell culture dish assembly 5 is connected to the limiting sliding groove 4 in a sliding mode, the cell culture dish assembly 5 is arranged to bear cell propagation, the top end of the cross-shaped partition plate 3 is connected with the sealing cover 7 through the threaded bolt 6 in a threaded mode, the sealing cover 7 is arranged to play a role of sealing the cell culture dish assembly 5, the constant-temperature cover assembly 8 is sleeved on the outer portion of the constant-temperature base assembly 1, a constant-temperature place is provided for cell culture through the arrangement of the constant-temperature cover assembly 8, cell propagation is promoted, the survival rate is improved, external bacteria can be isolated, the dustproof effect is achieved, the recording plate 9 is fixedly connected to the surface of the cell culture dish assembly 5, and cell propagation conditions of different time periods are registered through the arrangement of the recording plate 9, the cell culture technicians can conveniently count the cell culture data;

the constant temperature base assembly 1 comprises a temperature control base 101, a constant temperature condition is provided for cell culture through the arrangement of the temperature control base 101, the cell propagation efficiency is increased, a clamping groove 102 is formed in the periphery of the upper surface of the temperature control base 101, the clamping groove 102 is arranged to facilitate clamping of the constant temperature cover assembly 8, an anti-slip mat 103 is fixedly connected to the bottom of the temperature control base 101, the anti-slip effect is achieved through the arrangement of the anti-slip mat 103, a sealing ring is sleeved inside the clamping groove 102, and the sealing ring is arranged to achieve a sealing effect;

the cell culture dish assembly 5 comprises a cell culture dish 501, the cell culture dish 501 is arranged to play a role in bearing cell propagation, the surface of the cell culture dish 501 is in threaded connection with a handle 502, the cell culture dish 501 is pulled by the handle 502, the bottom of the cell culture dish 501 is fixedly connected with a limiting slide block 503, the limiting slide block 503 is connected to the inside of a limiting slide groove 4 in a sliding manner, the number of the cell culture dishes 501 is four, the buffering function is achieved by the arrangement of the limiting slide block 503, and the effects of arranging a plurality of culture grooves and increasing the cell culture speed are achieved by the arrangement of the four cell culture dishes 501;

the constant temperature cover assembly 8 comprises a constant temperature cover 801, the constant temperature cover 801 is arranged to achieve constant temperature sealing and prevent external bacteria from entering, a cover knob 802 is fixedly connected to the center of a circle on the upper surface of the constant temperature cover 801, and the constant temperature cover 801 is clamped with the upper end of the constant temperature base assembly 1;

the closed cover 7 is made of transparent glass, the transparent glass is arranged to facilitate observation of cells by cell culture technicians, so that statistics can be carried out, and the bottom end of the closed cover 7 is clamped in the constant-temperature base assembly 1;

the inside fixedly connected with stopper of spacing spout 4 through the setting of stopper, has played spacing effect, and the material of stopper is the rubber material, through the setting of rubber material, has played the effect of anticollision buffering.

The utility model discloses in, the device's working procedure as follows:

1. when a cell culture technician cultures cells, firstly, the limiting slide block 503 at the bottom end of the cell culture dish 501 needs to be placed in the limiting slide groove 4, the sealing cover 7 is fixed at the top end of the cross-shaped partition plate 3 through the threaded bolt 6 in a threaded manner, then the handle 502 on the surface of the cell culture dish 501 can be pulled out to pull out the cell culture dish 501, cell culture dish 501 is pushed in after cell liquid required to be cultured is dripped in, and the sealing cover 7 is made of transparent glass, so that the cell culture technician can observe and count the propagation condition of the cells through the transparent sealing cover 7 without opening the device, and the influence of bacteria and dust in the air on cell propagation is avoided;

2. finally, the constant temperature cover 801 is placed on the constant temperature base assembly 1 by lifting the cover button 802, and the bottom end of the constant temperature cover 801 is clamped into the clamping groove 102 for closed constant temperature storage, so that the propagation speed of cells is increased.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A cell culture container, comprising a thermostatic base assembly (1), characterized in that: fixedly connected with culture dish base (2) is located in the axle center of constant temperature base subassembly (1) upper surface, the last fixed surface of culture dish base (2) is connected with cross baffle (3), spacing screw hole has been seted up in the axle center department of cross baffle (3), the inside of cross baffle (3) is opened and is all been equipped with spacing spout (4), the inside sliding connection of spacing spout (4) has cell culture dish subassembly (5), there is closing cap (7) on the top of cross baffle (3) through threaded bolt (6) threaded connection, constant temperature lid subassembly (8) have been cup jointed to the outside of constant temperature base subassembly (1), the fixed surface of cell culture dish subassembly (5) is connected with recording sheet (9).

2. A cell culture vessel according to claim 1 wherein: constant temperature base subassembly (1) is including accuse temperature base (101), draw-in groove (102) have been seted up to the periphery of accuse temperature base (101) upper surface, the bottom fixedly connected with slipmat (103) of accuse temperature base (101), the sealing washer has been cup jointed to the inside of draw-in groove (102).

3. A cell culture vessel according to claim 1 wherein: cell culture dish subassembly (5) are including cell culture dish (501), the surperficial threaded connection of cell culture dish (501) has handle (502), the bottom fixedly connected with spacing slider (503) of cell culture dish (501), spacing slider (503) sliding connection is in the inside of spacing spout (4), the quantity of cell culture dish (501) is four.

4. A cell culture vessel according to claim 1 wherein: constant temperature lid subassembly (8) are including constant temperature lid (801), the centre of a circle department fixedly connected with of constant temperature lid (801) upper surface covers knob (802), constant temperature lid (801) joint is in the upper end of constant temperature base subassembly (1).

5. A cell culture vessel according to claim 1 wherein: the material of airtight lid (7) is transparent glass, the bottom joint of airtight lid (7) is in the inside of constant temperature base subassembly (1).

6. A cell culture vessel according to claim 1 wherein: the inside fixedly connected with stopper of spacing spout (4), the material of stopper is the rubber material.