CN212426032U - Disposable culture dish with separation structure - Google Patents
Disposable culture dish with separation structure Download PDFInfo
- Publication number
- CN212426032U CN212426032U CN202020475490.1U CN202020475490U CN212426032U CN 212426032 U CN212426032 U CN 212426032U CN 202020475490 U CN202020475490 U CN 202020475490U CN 212426032 U CN212426032 U CN 212426032U
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- China
- Prior art keywords
- culture dish
- dish body
- hole
- sealing
- disposable
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- 238000000926 separation method Methods 0.000 title claims abstract description 11
- 238000007789 sealing Methods 0.000 claims abstract description 38
- 238000002347 injection Methods 0.000 claims abstract description 32
- 239000007924 injection Substances 0.000 claims abstract description 32
- 238000005192 partition Methods 0.000 claims abstract description 12
- 241000894006 Bacteria Species 0.000 claims abstract description 5
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 239000007779 soft material Substances 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010030 laminating Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000007493 shaping process Methods 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 1
- 238000004115 adherent culture Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Images
Abstract
The utility model relates to a disposable culture dish with a separation structure, which comprises a culture dish body and a sealing cover; n partition plates are arranged in the culture dish body in a radial mode, are as high as the dish wall of the culture dish body, equally divide the volume space of the culture dish body into N sub-volume spaces with the same sectional area, M groups of adjusting holes are arranged on the sealing cover, wherein M is less than or equal to N, each group of adjusting holes comprise an air adjusting hole and an injection hole, and a bacterium filtering and air permeable film is arranged on the inner side of each air adjusting hole and used for providing an aerobic culture environment for the corresponding sub-volume space on the culture dish body; the injection ring sleeve is made of soft materials, and a ring hole matched with the injector is formed in the injection ring sleeve. The utility model discloses effectively separate the culture dish body and carry out contrast culture with the convenience, reduced the experimental expense cost to guarantee the uniformity and the controllability of internal environment in the culture dish body.
Description
Technical Field
The utility model relates to a culture dish and relevant device technical field, concretely relates to disposable culture dish with partition structure.
Background
In the field of biological culture, a culture dish is a laboratory vessel for microbial or cell culture, consisting of a planar dish-shaped bottom and a cover, generally made of glass or plastic, and can be used for the culture of plant materials, microorganisms and for the adherent culture of animal cells. The glass culture dish is usually a culture dish for multiple use, and the glass culture dish needs to be fully cleaned to reduce the pollution of impurities or mixed bacteria when in use, so that the use is troublesome; for the reasons, disposable culture dishes made of polyethylene or other plastic materials are also used in the market, and the culture dishes are of a preformed sterilization structure and have the advantages of convenience in use, easiness in treatment and the like.
However, the disposable culture dish in the prior art is too simple in structure, and when a plant cultivation contrast experiment is carried out and a contrast experiment needs to be carried out, a plurality of disposable culture dishes are needed for contrast operation; when different culture dishes are used for operation culture, on one hand, the number of the required culture dishes is large, which can increase the experiment cost; on the other hand, slight differences exist between the culture environments of corresponding single individual culture dishes, and the comparison process influences the data accuracy of the experiment. In the actual comparative culture process, certain different choices exist for air circulation and addition of some reagents or nutrients, and the different choices directly influence the test result, but the test result is also influenced by frequent uncapping operations of the culture dish.
SUMMERY OF THE UTILITY MODEL
The technical problem solved by the utility model is to provide a disposable culture dish with a separation structure to solve the defects in the technical background.
The utility model provides a technical problem adopt following technical scheme to realize:
a disposable culture dish with a separation structure comprises a culture dish body and a sealing cover matched with the culture dish body; n partition plates are arranged in the culture dish body in a radial mode, are as high as the dish wall of the culture dish body, equally divide the volume space of the culture dish body into N sub-volume spaces with the same sectional area, M groups of adjusting holes are arranged on the sealing cover, wherein M is less than or equal to N, each group of adjusting holes comprise an air adjusting hole and an injection hole, and a bacterium filtering and air permeable film is arranged on the inner side of each air adjusting hole and used for providing an aerobic culture environment for the corresponding sub-volume space on the culture dish body; the injection ring sleeve is made of soft materials, and a ring hole matched with the injector is formed in the injection ring sleeve.
By way of further limitation, the culture dish body is a circular dish body, a square dish body or other dish bodies with regular cross sections.
As a further limitation, a sealing structure is formed between the culture dish body and the sealing cover, and the sealing structure is one or a combination of a sealing groove, a liquid sealing groove, a sealing strip and a sealing ring.
As further limitation, the section of the outer cylindrical surface of the culture dish body and the inner surface of the sealing cover are inclined edges matched with each other, and an included angle between each inclined edge and the horizontal plane ranges from 75 degrees to 90 degrees.
And as a further limitation, a sealing strip is formed on the upper surface of the partition plate, and the sealing strip is attached to the inner surface of the sealing cover after the sealing cover seals the culture dish body so as to ensure the sealing property between the adjacent sub-volume spaces.
As a further limitation, the air adjustment hole is closed by a detachable plug.
As a further limitation, the injection hole is closed by a detachable rubber plug so as to close the injection hole after the injection ring sleeve is detached from the injection hole.
The cross-sectional area of the air adjusting hole is 1/20-1/5 of the cross-sectional area of the corresponding sub-volume space.
Has the advantages that: the utility model discloses a disposable culture dish simple structure, reasonable in design, the partition structure that it has can carry out the contrast culture of multiunit test thing in single culture dish to guarantee the uniformity of culture environment, overcome the selectivity gas permeability problem of culture dish among the prior art through guaranteeing sealed effect and setting up the regulation hole simultaneously, and can solve traditional culture dish and need uncap the defect of carrying out the content and pour into, saved the operation of opening, closing of closing cap, reduced the risk of culture dish pollution; labor and time are saved, the test cost and the risk can be effectively reduced, and the precision of test data is improved.
Drawings
Fig. 1 is a detailed top view of the preferred embodiment of the present invention.
Fig. 2 is a sectional detail view of the preferred embodiment of the present invention.
Wherein: 1. a closure cap; 2. air adjusting holes; 3. filtering bacteria and ventilating; 4. an injection hole rubber plug; 5. a partition plate; 6. a sealing strip; 7. a culture dish body; 8. a sub-volume space; 9. a seal ring; 10. a rubber plug with an air adjusting hole; 11. an injection ring sleeve; 12. a syringe.
Detailed Description
In order to make the technical means, creation features, achievement purposes and functions of the present invention easy to understand and understand, the present invention is further explained by combining with the specific drawings.
Referring to fig. 1 and 2, in the present embodiment, the disposable culture dish is made of plastic material and is injection molded, and includes a separable culture dish body 7 and a closing cover 1, wherein the culture dish body 7 is a circular dish body, and meanwhile, the culture dish body 7 may also be a square dish body or other dish bodies with regular cross-sections. The section of the outer cylinder of the culture dish body 7 and the inner surface of the sealing cover 1 is a matched bevel edge, and the included angle between the bevel edge and the horizontal plane is 85 degrees, the sealing cover 1 arranged on the bevel edge can have better laminating performance with the culture dish body 7, the operation problem that the sealing cover 1 needs to be completely covered on the culture dish body 7 is avoided, and meanwhile, the sealing ring 9 arranged on the outer cylinder of the culture dish body 7 can have better laminating performance to improve the sealing effect.
And separate into three sectorial subvolumes space 8 through three baffle 5 that are radial setting in the culture dish body 7 of this embodiment, these three subvolumes space 8 are independent space, can regard as three culture space respectively, cultivate three different cultures respectively, and in order to guarantee the relative independence of three subvolumes space 8, baffle 5 is as high as the dish wall of culture dish body, and the shaping has sealing strip 6 on the upper surface of baffle 5, sealing strip 6 is the soft material shaping that the upper portion is the Y shape, after closing lid 1 is covered, laminate in order to guarantee the leakproofness between the adjacent subvolumes space on the internal surface of closing lid 1.
And still be provided with a set of regulation hole on closing cap 1 of this embodiment, the regulation hole is including adjusting gas pocket 2 and injection hole, should adjust gas pocket 2 to the round hole, the cross sectional area of adjusting gas pocket 2 is 1/15 of the cross sectional area of the sub-volume space that corresponds, and still be provided with in the inboard of adjusting gas pocket 2 and strain fungus ventilated membrane 3, guarantee ventilation effect on the one hand, can also prevent miscellaneous fungus pollution simultaneously, the inboard upper portion of adjusting gas pocket 2 is provided with the shoulder hole, and be provided with detachable adjusting gas pocket plug 10 in the shoulder hole position and carry out the selectivity and seal to adjusting gas pocket 2.
An injection hole is arranged on the inner side of the sealing cover 1 corresponding to the air adjusting hole 2, a stepped hole is also arranged on the upper part of the inner side of the injection hole, when materials are injected, the injection ring sleeve 11 is sleeved on the stepped hole at the position of the injection hole, then the injector 12 is sleeved on a ring hole which is arranged in the center of the injection ring sleeve 11 and matched with the injector 12, and then materials with corresponding quantity are injected; after the injection is completed, the injection ring sleeve 11 and the injector 12 are integrally taken out to prevent residues from polluting the position of the injection hole and further polluting the culture dish body, and after the injection ring sleeve 11 is taken out, the injection hole is directly sealed through the injection hole rubber plug 4, so that the relative independence of the corresponding sub-volume space can be continuously ensured.
In this embodiment, because the closing cap 1 of selection has only set up a set of regulation hole, therefore a set of air feed and material adjustment in the three group of sub-volume spaces 8 to the culture dish body 7, adjust other three group of sub-volume spaces 8 if need, can choose for use the closing cap 1 of taking two sets of or three group's regulation holes to come to cover the culture dish body 7 and close to rotate closing cap 1, the regulation hole that the adjustment corresponds can with corresponding sub-volume space 8's position relation.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that various changes, modifications and/or alterations to the present invention may be made by those skilled in the art after reading the technical disclosure of the present invention, and all such equivalents may fall within the scope of the present invention as defined by the appended claims.
Claims (10)
1. A disposable culture dish with a separation structure is characterized by comprising a culture dish body and a sealing cover matched with the culture dish body; n partition plates are arranged in the culture dish body in a radial mode, are as high as the dish wall of the culture dish body, equally divide the volume space of the culture dish body into N sub-volume spaces with the same sectional area, M groups of adjusting holes are arranged on the sealing cover, wherein M is less than or equal to N, each group of adjusting holes comprise an air adjusting hole and an injection hole, and a bacterium filtering and air permeable film is arranged on the inner side of each air adjusting hole and used for providing an aerobic culture environment for the corresponding sub-volume space on the culture dish body; the injection ring sleeve is made of soft materials, and a ring hole matched with the injector is formed in the injection ring sleeve.
2. The disposable culture dish with the separation structure according to claim 1, wherein the culture dish body is a circular dish body, a square dish body or other dish bodies with regular cross sections.
3. The disposable culture dish with a partition structure according to claim 1, wherein a sealing structure is formed between the culture dish body and the closing cover.
4. The disposable culture dish with a separation structure according to claim 3, wherein the sealing structure is one or a combination of a sealing groove, a liquid sealing groove, a sealing strip and a sealing ring.
5. The disposable culture dish with a partition structure according to claim 1, wherein the section of the outer cylindrical surface of the culture dish body and the section of the inner surface of the closing cover are matched inclined edges.
6. The disposable culture dish with a separation structure according to claim 5, wherein the inclined edge is 75-90 ° from the horizontal plane.
7. The disposable culture dish with the separation structure according to claim 1, wherein a sealing strip is formed on the upper surface of the partition plate, and the sealing strip is attached to the inner surface of the sealing cover after the sealing cover closes the culture dish body.
8. The disposable culture dish with a partition structure of claim 1, wherein the air adjusting hole is closed by a detachable rubber plug.
9. The disposable culture dish with a partition structure of claim 1, wherein the injection hole is closed by a detachable plug which closes the injection hole after the injection ring is removed from the injection hole.
10. The disposable culture dish with a separation structure according to claim 1, wherein the cross-sectional area of the air-adjusting hole is 1/20-1/5 of the cross-sectional area of the corresponding sub-volume space.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020475490.1U CN212426032U (en) | 2020-04-03 | 2020-04-03 | Disposable culture dish with separation structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020475490.1U CN212426032U (en) | 2020-04-03 | 2020-04-03 | Disposable culture dish with separation structure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212426032U true CN212426032U (en) | 2021-01-29 |
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ID=74295257
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020475490.1U Active CN212426032U (en) | 2020-04-03 | 2020-04-03 | Disposable culture dish with separation structure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212426032U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113046231A (en) * | 2021-03-23 | 2021-06-29 | 广东轻工职业技术学院 | Reduce bacterial solution inoculation device that other bacterial sneaked into |
-
2020
- 2020-04-03 CN CN202020475490.1U patent/CN212426032U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113046231A (en) * | 2021-03-23 | 2021-06-29 | 广东轻工职业技术学院 | Reduce bacterial solution inoculation device that other bacterial sneaked into |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231107 Address after: 7th and 8th Floor, Building 2, No. 7 Juxing Road, Xianshuigu Town, Jinnan District, Tianjin 300350 Patentee after: Baioupai (Tianjin) Biotechnology Co.,Ltd. Address before: Room 1468, Xiangshui hetianxia, No.1, Shaoshan South Road, Yuhua District, Changsha, Hunan 410000 Patentee before: Zhong Shuai |
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| TR01 | Transfer of patent right |