CN212925050U

CN212925050U - Novel cell culture medium

Info

Publication number: CN212925050U
Application number: CN202020991855.6U
Authority: CN
Inventors: 吕青兰; 谭斯品; 邹江; 蒋磊; 刘可; 张华莉; 肖献忠
Original assignee: Central South University
Current assignee: Central South University
Priority date: 2020-06-03
Filing date: 2020-06-03
Publication date: 2021-04-09
Anticipated expiration: 2030-06-03

Abstract

The utility model discloses a novel cell culture medium, concretely relates to cell culture technical field, which comprises a base bod, the base member is trapezoidal form, the base member inner chamber is equipped with the culture seat, the culture seat is trapezoidal form, culture seat and base member activity joint, the culture seat inner chamber is equipped with cultivates the dish, it is trapezoidal form to cultivate the dish, cultivate dish and culture seat activity joint, the base member outer wall is equipped with first air vent, it is equipped with the second air vent to cultivate the seat outer wall. The utility model discloses a set up the culture seat, be equipped with the second handle on the culture seat, rotatory second handle, the second handle can drive the culture seat and rotate, because the base member sets up to transparent, so can be accurate corresponding second handle and first air vent, when rotating the second handle directly over first air vent, first ventilation and second air vent intercommunication this moment, when the second handle is not directly over first air vent, first air vent and second air vent do not communicate this moment.

Description

Novel cell culture medium

Technical Field

The utility model relates to a cell culture technical field, more specifically say, the utility model relates to a novel cell culture medium.

Background

Currently, cell culture refers to a culture technique in which cells are taken out from tissues in vivo to simulate the in vivo growth environment, and the cells are grown and propagated in a growth period under the conditions of sterility, proper temperature, pH value and certain nutrition, and the structure and function of the cells are maintained. One of the key points of the cell in vitro culture technology is a cell culture medium, and the ideal cell culture medium can simultaneously meet all the requirements of pH, osmotic pressure, nutrient substances and regulating substances required by the cell in vitro culture.

In the scientific research cell culture experiment process, because the culture medium is rich in nutrient substances and is easy to be polluted or deteriorate, the culture medium needs to be cultured in a corresponding incubator in the culture process, and when an experimenter takes out the culture medium from the incubator, if the experiment is not started immediately, microorganisms are easily introduced, so that the pollution of the whole culture medium is caused, and the culture medium can not be placed in the external environment for a long time.

SUMMERY OF THE UTILITY MODEL

In order to overcome the above-mentioned defect of prior art, the embodiment of the utility model provides a novel cell culture medium, when the culture medium is when the incubator, through the first air vent of rotatory second handle control and second air vent intercommunication, the air admission culture medium in the incubator, the culture medium begins to cultivate the cell, when the culture medium is taken out the incubator, through the first air vent of rotatory second handle control and second air vent not intercommunication, the outside air can not get into the culture medium, so can not make the culture medium pollute.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a novel cell culture medium, includes the base member, the base member is trapezoidal form, the base member inner chamber is equipped with cultivates the seat, it is trapezoidal form to cultivate the seat, cultivate seat and base member activity joint, it is equipped with cultivates the dish to cultivate the seat inner chamber, it is trapezoidal form to cultivate the dish, cultivate dish and cultivates seat activity joint, base member outer wall bilateral symmetry is equipped with first air vent, it is equipped with the second air vent to cultivate seat outer wall bilateral symmetry, the second air vent is corresponding with first air vent.

Preferably, the base body top is equipped with the base lid, base lid and base body activity joint, fixedly connected with lid stopper in the middle of the bottom of base lid, the lid stopper cooperatees with base body top opening.

Preferably, a circle of rubber ring is fixedly arranged on the side wall of the inner cavity of the base cover.

Preferably, a sealing ring is arranged on the edge of one side of the inner cavity of the first vent hole, which is close to the culture seat, and the sealing ring is fixedly connected with the base body.

Preferably, symmetrical first handles are fixedly arranged on two sides of the top edge of the base cover.

Preferably, two symmetrical second handles are fixedly arranged on two sides of the edge of the top of the culture seat, and the second handles are arranged right above the second vent holes.

Preferably, symmetrical third handles are fixedly arranged on two sides of the top edge of the culture tray.

Preferably, the substrate is transparent.

The utility model discloses a technological effect and advantage:

1. the utility model discloses a setting up the culture seat, being equipped with the second handle on the culture seat, rotatory second handle, the second handle can drive the culture seat and rotate, because the base member sets up to transparent, so can be accurate correspond second handle and first air vent, when rotating the second handle directly over the first air vent, first ventilation and second air vent intercommunication this moment, gas can get into the culture medium, when the second handle is not directly over the first air vent, first air vent and second air vent do not communicate this moment, gas can't get into the culture medium;

2. the utility model discloses a be equipped with the sealing ring at a side edge that first air vent inner chamber is close to the culture base, when first air vent and second air vent do not communicate, prevent the air from getting into the culture medium from the gap between culture base and the base member in and polluting the culture medium.

Drawings

Fig. 1 is a schematic overall sectional structural view of the present invention.

Fig. 2 is a schematic overall side view of the present invention.

FIG. 3 is a schematic side view of the culture seat of the present invention.

FIG. 4 is a schematic top view of the culture tray of the present invention.

FIG. 5 is a front view of the structure of the culture tray of the present invention.

The reference signs are: 1 substrate, 2 culture seats, 3 culture plates, 4 first vent holes, 5 second vent holes, 6 substrate covers, 7 cover plugs, 8 rubber rings, 9 sealing rings, 10 first handles, 11 second handles and 12 third handles.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

According to the novel cell culture medium that fig. 1-5 shows, including base member 1, base member 1 is trapezoidal form, 1 inner chamber of base member is equipped with culture tray 2, culture tray 2 is trapezoidal form, culture tray 2 and 1 activity joint of base member, 2 inner chambers of culture tray are equipped with cultivates dish 3, it is trapezoidal form to cultivate dish 3, cultivate dish 3 and the 2 activity joints of culture tray, 1 outer wall bilateral symmetry of base member is equipped with

first air vent

4, 2 outer wall bilateral symmetry of culture tray are equipped with second air vent 5, second air vent 5 is corresponding with first air vent 4.

Further, base lid 6 is equipped with at 1 top of base member, base lid 6 and 1 activity joint of base member, fixedly connected with lid stopper 7 in the middle of the bottom of base lid 6, lid stopper 7 cooperatees with 1 top opening of base member.

Furthermore, a circle of rubber ring 8 is fixedly arranged on the side wall of the inner cavity of the base cover 6, so that the base cover 6 and the base body 1 can be tighter.

Further, a sealing ring 9 is arranged on the edge of one side of the inner cavity of the first vent hole 4, which is close to the culture base 2, the sealing ring 9 is fixedly connected with the base body 1, and when the first vent hole 4 is not communicated with the second vent hole 5, air is prevented from entering the culture medium, so that pollution is caused.

Furthermore, two sides of the top edge of the base cover 6 are fixedly provided with symmetrical first handles 10, so that the base cover 6 can be conveniently opened.

Further, 2 fixed second handles 11 that are equipped with the symmetry in edge both sides in top of culture seat, second handle 11 sets up directly over second vent 5, conveniently mentions culture seat 2, and can indicate first vent whether with second vent intercommunication.

Furthermore, two symmetrical third handles 12 are fixedly arranged on two sides of the top edge of the culture tray 3, so that the culture tray is convenient to lift.

Further, the base body 1 is transparent, and the second handle 12 can be accurately arranged corresponding to the first ventilation hole 4.

The utility model discloses the theory of operation: when cell culture is to be carried out, the base cover 6 is taken out through the first handle 10, the culture seat 2 and the culture tray 3 are taken out through the second handle 11, culture solution and cells to be cultured are added on the culture tray 3, then the culture seat 2 is placed into a bottle, the first vent hole 4 is not communicated with the second vent hole 5, the culture tray 3 is placed into the culture seat 2, the base cover 6 is covered, then the substrate 1 is placed into an incubator, the base cover 6 is opened when the incubator is placed, the second handle 11 is rotated, the second handle 11 drives the culture seat 2 to rotate, because the substrate 1 is transparent, the second handle 12 can accurately correspond to the first vent hole 4, namely, the first vent hole 4 is communicated with the second vent hole 5, when the second handle 11 is rotated to be positioned above the first vent hole 4, the first vent hole 4 is communicated with the second vent hole 5, cover substrate lid 6, begin to cultivate the cell, when the laboratory technician need take out the incubator with the culture medium, open substrate lid 6 in the incubator, rotatory second handle 12, make first breather pipe 4 stagger with second breather pipe 5, cover substrate lid 6, take out base member 1, because first breather pipe 4 does not communicate with second breather pipe 5, at this moment the outside air can not get into the cell in the pollution culture dish 3 in the culture medium, just can take out the incubator with the culture medium when the later stage is experimental again, conveniently experiment at any time, the neutral period (can be long or short)) culture medium between the use is taken out to the culture medium like this can not be contaminated, along with getting and using.

The points to be finally explained are: first, in the description of the present application, it should be noted that, unless otherwise specified and limited, the terms "mounted," "connected," and "connected" should be understood broadly, and may be a mechanical connection or an electrical connection, or a communication between two elements, and may be a direct connection, and "upper," "lower," "left," and "right" are only used to indicate a relative positional relationship, and when the absolute position of the object to be described is changed, the relative positional relationship may be changed;

secondly, the method comprises the following steps: in the drawings of the disclosed embodiments of the present invention, only the structures related to the disclosed embodiments are referred to, and other structures can refer to the common design, and under the condition of no conflict, the same embodiment and different embodiments of the present invention can be combined with each other;

and finally: the above description is only for the preferred embodiment of the present invention and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A novel cell culture medium, comprising a substrate (1), characterized in that: base member (1) is the trapezoidal form, base member (1) inner chamber is equipped with cultivates seat (2), it is the trapezoidal form to cultivate seat (2), cultivate seat (2) and base member (1) activity joint, it is equipped with cultivates dish (3) to cultivate seat (2) inner chamber, it is the trapezoidal form to cultivate dish (3), cultivate dish (3) and cultivate seat (2) activity joint, base member (1) outer wall bilateral symmetry is equipped with first air vent (4), it is equipped with second air vent (5) to cultivate seat (2) outer wall bilateral symmetry, second air vent (5) are corresponding with first air vent (4).

2. A novel cell culture medium according to claim 1, wherein: the base body (1) top is equipped with base lid (6), base lid (6) and base body (1) activity joint, fixedly connected with lid stopper (7) in the middle of the bottom of base lid (6), lid stopper (7) cooperate with base body (1) top opening.

3. A novel cell culture medium according to claim 2, wherein: and a circle of rubber ring (8) is fixedly arranged on the side wall of the inner cavity of the base cover (6).

4. A novel cell culture medium according to claim 1, wherein: a sealing ring (9) is arranged on the edge of one side of the inner cavity of the first vent hole (4) close to the culture seat (2), and the sealing ring (9) is fixedly connected with the base body (1).

5. A novel cell culture medium according to claim 2, wherein: and symmetrical first handles (10) are fixedly arranged on two sides of the top edge of the base cover (6).

6. A novel cell culture medium according to claim 1, wherein: the two sides of the edge of the top of the culture seat (2) are fixedly provided with symmetrical second handles (11), and the second handles (11) are arranged right above the second vent holes (5).

7. A novel cell culture medium according to claim 1, wherein: and symmetrical third handles (12) are fixedly arranged on two sides of the top edge of the culture tray (3).

8. A novel cell culture medium according to claim 1, wherein: the substrate (1) is transparent.