CN212077079U - a cell culture flask - Google Patents

Abstract

The utility model discloses a cell culture bottle, including bottle and cork, be provided with the cavity in the bottle, the bottom evenly is provided with a plurality of cell culture boards in the bottle, the inside cavity of cell culture board, cell culture board bottom and cavity intercommunication, bottle upper portion are provided with two bottlenecks, bottleneck and cork cooperate, are provided with permeability cell and pipette on the cork of left side, are provided with on the cork of right side and annotate liquid pipe and permeability cell, and this cell culture bottle can effectively solve the cell culture process and receive the limited problem of pollution, single cell culture bottle culture cell volume easily, creates comfortable ambient temperature for the cell of cultivateing.

Description

a cell culture flask

technical field

The utility model relates to a cell culture device, in particular to a cell culture bottle.

Background technique

Cell culture refers to a method that simulates the in vivo environment (sterility, suitable temperature, pH and certain nutrient conditions, etc.) in vitro to make it survive, grow, reproduce and maintain its main structure and function.

The cell culture flask in the prior art has a simple structure and cannot create a comfortable ambient temperature for the cultured cells. During the process of adding the culture solution and discharging the waste solution, it is usually necessary to open the container lid, which is easily affected by the external environment, resulting in the failure of the cell culture operation, and the cell culture flask usually only grows cells on the inner wall of the cell culture flask. The amount of cells cultured in a culture flask is limited, so culturing mammalian cells requires the use of a large number of cell culture flasks, which is not conducive to mammalian cell culture.

Utility model content

The utility model aims at providing a cell culture flask in view of the technical defects of the prior art, which can effectively solve the problems that the cell culture process is easily polluted and the amount of cultured cells in a single cell culture flask is limited, and create a comfortable environment temperature for the cultured cells .

To achieve the above object, the utility model provides the following technical solutions:

A cell culture bottle includes a bottle body and a cork, the bottle body is provided with a cavity, the bottom of the bottle body is provided with a drain pipe, the drain pipe is provided with a drain valve, and the left upper part of the bottle body is provided with a water inlet pipe, so the The water inlet pipe is provided with a water inlet valve, a plurality of cell culture plates are evenly arranged at the bottom of the bottle body, the interior of the cell culture plate is hollow, the bottom of the cell culture plate is communicated with the cavity, the outer side of the bottle body is provided with a thermal insulation layer, and the upper part of the bottle body is provided with There are two bottle mouths, the bottle mouth and the cork stopper are matched, the left cork stopper is provided with a vent tube and a liquid suction tube, the vent tube is located on the left side of the suction tube, and the right cork stopper is provided with a liquid injection The liquid injection pipe is located on the left side of the ventilation pipe, and the ventilation pipe is provided with a hydrophobic gas permeable film. One end of the ventilation pipe is connected to the atmosphere, and the other end is connected to the inside of the bottle body. The lower part of the suction pipe is directly connected to the bottom of the bottle body. The upper part of the suction pipe is provided with a small water pump, the bottom of the liquid injection pipe is connected to the inside of the bottle body, the upper part is provided with a liquid injection valve, and the top of the liquid injection pipe is fixedly connected to the conical port.

In this embodiment: the material of the thermal insulation layer is polyurethane foam.

In this embodiment: the ventilation tube, the liquid suction tube, the liquid injection tube and the cork are detachably connected.

The beneficial effects of the utility model are as follows: (1) the inner surface of the bottle and the outer surface of the cell culture plate are modified to support cell adhesion and growth, and the culture area is increased; (2) the cell liquid and the culture liquid are injected through the liquid injection tube, Ventilation through the hydrophobic gas permeable membrane eliminates the need to open the bottle cap and prevent external bacterial contamination; (3) After the cells have grown for a certain period of time, the waste liquid is extracted through a pipette, and the cells attached to the inner surface of the bottle and the outer surface of the cell culture plate are not affected. It is easy to fall; (4) When the temperature is high, the cold water is injected into the cavity through the water inlet pipe, and when the temperature is low, the warm water is injected into the cavity through the water inlet pipe to create a comfortable temperature for cell reproduction.

Description of drawings

Fig. 1 is the structural representation of the utility model;

Fig. 2 is a top view at Fig. 1A-A;

Reference signs: bottle body 1, cell culture plate 2, cavity 3, insulation layer 4, pipette 5, small water pump 6, hydrophobic gas permeable membrane 7, gas permeable pipe 8, liquid injection pipe 9, liquid injection valve 10, cone Shaped mouth 11, cork 12, water inlet pipe 13, water inlet valve 14, drain pipe 15, drain valve 16, bottle mouth 17.

Detailed ways

The present invention will be described in detail below with reference to the accompanying drawings. As shown in Figures 1-2, a cell culture bottle includes a bottle body 1 and a cork stopper 12. The bottle body 1 is provided with a cavity 3, and the bottle body 1 The bottom is provided with a drain pipe 15, the drain pipe 15 is provided with a drain valve 16, the upper left part of the bottle body 1 is provided with a water inlet pipe 13, the water inlet pipe 13 is provided with a water inlet valve 14, a water inlet pipe 13 and a drain pipe 15 It is convenient to replace the liquid in the cavity 3 and to control the volume of the liquid in the cavity 3 .

In this embodiment, several cell culture plates 2 are evenly arranged at the inner bottom of the bottle body 1 , the interior of the cell culture plate 2 is hollow, the bottom of the cell culture plate 2 is communicated with the cavity 3 , and the inner surface of the bottle body 1 is connected to the cell culture plate 2 The outer surface has been modified to allow cells to adhere, divide and grow.

In this embodiment: the outer side of the bottle body 1 is provided with a thermal insulation layer 4, the upper part of the bottle body 1 is provided with two bottle mouths 17, the cork stopper 12 is matched with the bottle mouth 17, and the left cork stopper 12 is provided with a ventilation pipe 8 and the suction pipe 5, the breathable pipe 8 is located on the left side of the suction pipe 5, the right cork 12 is provided with a liquid injection pipe 9 and a ventilation pipe 8, and the liquid injection pipe 9 is located on the left side of the ventilation pipe 8, The breathable tube 8 is provided with a hydrophobic breathable membrane 7, one end of the breathable tube 8 is connected to the atmosphere, and the other end is connected to the inside of the bottle body 1, which is convenient for exchanging oxygen and carbon dioxide with the outside world. The upper part of the liquid pipe 5 is provided with a small water pump 6, which can absorb the waste liquid in the culture bottle. The bottom of the liquid injection pipe 9 is connected to the inside of the bottle body 1, the upper part is provided with a liquid injection valve 10, and the top of the liquid injection pipe 9 is fixedly connected to the conical port 11. , adding cell liquid and culture liquid through the liquid injection tube 9, it is not easy to spill outside and pollute the laboratory environment.

In this embodiment, the material of the thermal insulation layer 4 is polyurethane foam, which has a good thermal insulation effect.

In this embodiment, the ventilation pipe 8, the liquid suction pipe 5, the liquid injection pipe 9 and the cork stopper 12 are detachably connected, which is convenient for regular cleaning and sterilization in the later period.

The working principle of the utility model is as follows: the inner surface of the bottle body 1 and the outer surface of the cell culture plate 2 are modified to support cell adhesion and growth, and the culture area is increased. The breathable membrane 7 is ventilated without opening the bottle cap to prevent external bacterial contamination. After the cells grow for a certain period of time, the waste liquid is extracted through the pipette 5, and the cells attached to the inner surface of the bottle body 1 and the outer surface of the cell culture plate 2 are not easy to fall off. When the temperature is high, cold water is injected into the cavity 3 through the water inlet pipe 13, and when the temperature is low, warm water is injected into the cavity 3 through the water inlet pipe 13 to create a comfortable temperature for cell reproduction.

The above are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the present invention. within the scope of protection of the utility model.

Claims (3)

1. A cell culture bottle, characterized in that: a bottle body (1) and a cork stopper (12) are included, a cavity (3) is provided in the bottle body (1), and a drain is provided at the bottom of the bottle body (1) Pipe (15), a drain valve (16) is arranged on the drain pipe (15), a water inlet pipe (13) is arranged on the upper left part of the bottle body (1), and a water inlet valve (13) is arranged on the water inlet pipe (13). 14), the inner bottom of the bottle body (1) is evenly provided with several cell culture plates (2), the interior of the cell culture plate (2) is hollow, the bottom of the cell culture plate (2) is communicated with the cavity (3), and the bottle body ( 1) An insulating layer (4) is provided on the outer side, and two bottle mouths (17) are arranged on the upper part of the bottle body (1), the bottle mouths (17) are matched with the cork stopper (12), and the left cork stopper (12) A vent pipe (8) and a liquid suction pipe (5) are provided on it, the vent pipe (8) is located on the left side of the liquid suction pipe (5), and the right cork stopper (12) is provided with a liquid injection pipe (9) and The ventilation pipe (8), the liquid injection pipe (9) is located on the left side of the ventilation pipe (8), the ventilation pipe (8) is provided with a hydrophobic gas permeable membrane (7), one end of the ventilation pipe (8) is connected to the atmosphere, and the other side is connected to the atmosphere. One end is connected to the inside of the bottle body (1), the lower part of the suction pipe (5) is directly connected to the bottom of the bottle body (1), the upper part of the suction pipe (5) is provided with a small water pump (6), and the bottom of the liquid injection pipe (9) The inside of the bottle body (1) is communicated, and a liquid injection valve (10) is arranged on the upper part, and the top of the liquid injection pipe (9) is fixedly connected to the conical port (11). 2. A cell culture flask according to claim 1, characterized in that: the material of the thermal insulation layer (4) is polyurethane foam. 3. A cell culture flask according to claim 1, characterized in that: the ventilation pipe (8), the liquid suction pipe (5), the liquid injection pipe (9) and the cork (12) are detachably connected.

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