CN211665114U

CN211665114U - Culture bottle suitable for 3D cultivates stem cell

Info

Publication number: CN211665114U
Application number: CN201922420036.6U
Authority: CN
Inventors: 阮光萍; 姚翔; 潘兴华; 庞荣清
Original assignee: 920th Hospital of the Joint Logistics Support Force of PLA
Current assignee: 920th Hospital of the Joint Logistics Support Force of PLA
Priority date: 2019-12-28
Filing date: 2019-12-28
Publication date: 2020-10-13
Anticipated expiration: 2029-12-28

Abstract

The utility model discloses a culture bottle suitable for 3D culture of stem cells, which comprises a culture bottle, wherein the culture bottle comprises a bottle body and a bottle cap, the bottle body comprises a bottle body and a bottleneck, the bottle body is cylindrical and hollow, the upper end of the bottle body is open, the open end of the bottle body is provided with a sealing cap, one end of the bottleneck is communicated with the bottle body, the end of the bottleneck far away from the bottle body is connected with the bottle cap, and the bottle body and the sealing cap are transparent; the bottom of the bottle body is provided with a baffle plate, the baffle plate and the bottle body form a water channel, and the bottom of the water channel is provided with a culture hole; the sealing cover is provided with a liquid inlet pipe, the liquid inlet end of the liquid inlet pipe is aligned to the center of the water channel, and the liquid outlet end of the liquid inlet pipe is positioned outside the bottle body; a liquid outlet pipe is arranged on the circumferential side of the bottle body, the liquid inlet end of the liquid outlet pipe is communicated with the tail end of the water channel, and the liquid outlet end of the liquid outlet pipe is positioned on the outer side of the bottle body; the stability of the cell growth environment is ensured by continuously providing fresh nutrient substances for the cells in the culture holes and taking away the metabolites of the cells.

Description

Culture bottle suitable for 3D cultivates stem cell

Technical Field

The utility model relates to a medical science experiment field, in particular to blake bottle suitable for 3D cultivates stem cell.

Background

Most of the current cell biology research is still carried out in two-dimensional planar culture, but scientists gradually find the limitation of 2D cell culture: the growth mode, morphology, differentiation, function and the like of cells in planar culture are obviously different from those under in vivo physiological conditions. 3D cell culture can provide the microenvironment that more closely lives the condition in vivo for the cell, can simulate physiological state better to obtain the experimental result more unanimous with the in vivo experiment.

At present, a microporous plate is generally used for culturing stem cells, the separated stem cells are respectively placed in U-shaped holes of the microporous plate, each U-shaped hole can be used for placing one stem cell, the stem cells are cultured into 3D cell balls, concentration gradients of oxygen, nutrients and metabolic waste exist in the cell balls, multiple characteristics of solid tissues can be simulated, and the three-dimensional stem cell culture method is an important 3D physiological model for researching solid tumor occurrence and stem cell differentiation. As the stem cells are cultured in the U-shaped holes, cell metabolites and required nutrients are more difficult to control along with the increase of the culture time, and the division and differentiation of the stem cells are influenced. Therefore, a culture device capable of ensuring the growth environment of stem cells to be stable is needed.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a blake bottle suitable for 3D cultivates stem cell, through constantly providing fresh nutrient substance and taking away the metabolite of cell for the downthehole cell of cultivateing, guarantee the stability of cell growth environment.

The above technical purpose of the present invention can be achieved by the following technical solutions:

a culture bottle suitable for 3D culture of stem cells comprises a culture bottle body and a bottle cap, wherein the bottle body comprises a bottle body and a bottle neck, the bottle body is cylindrical and hollow, the upper end of the bottle body is open, an openable sealing cap is arranged at the open end of the bottle body, one end of the bottle neck is communicated with the bottle body, one end of the bottle neck, far away from the bottle body, is in threaded connection with the bottle cap, and the bottle body and the sealing cap are both transparent;

the bottom center of the bottle body is provided with a baffle distributed in a vortex line, the baffle and the bottle body form a water channel, the bottom of the water channel is provided with a plurality of culture holes at equal intervals except the center of the water channel, the bottoms of the culture holes are hemispherical cambered surfaces, and the cambered surfaces are ultra-low adsorption surfaces; the sealing cover is provided with a liquid inlet pipe communicated with the inner cavity of the bottle body, the water outlet end of the liquid inlet pipe is positioned in the bottle body and aligned to the center of the water channel, and the water inlet end of the liquid inlet pipe is positioned outside the bottle body; the bottle is characterized in that a liquid outlet pipe communicated with an inner cavity of the bottle body is arranged on the circumferential side of the bottle body, a water inlet end of the liquid outlet pipe is communicated with one end, far away from the center, of the water channel, and a water outlet end of the liquid outlet pipe is positioned on the outer side of the bottle body.

The utility model discloses a further set up to: the vertical solid fixed ring that is provided with towards the bottle inner chamber of open end of bottle, sealed lid includes inner cup, enclosing cover and sealing washer, the outside of inner cup with gu fixed ring's inboard phase-match, the enclosing cover is located the upper end of inner cup is set up the open end of bottle, the sealing washer cover is established the inner cup outside and with gu fixed ring forms sealedly.

The utility model discloses a further set up to: the opening end of the bottle body is circular.

The utility model discloses a further set up to: the height difference between the liquid outlet end of the liquid inlet pipe and the bottle bottom is 1-5 mm.

The utility model discloses a further set up to: the bottleneck is arranged on the circumferential side of the bottle body, and the bottom surface of the bottleneck and the bottom of the bottle body form an included angle of 10-30 degrees.

The utility model discloses a further set up to: the bottle cap is an air hole type cap.

The utility model discloses a further set up to: and a filter membrane with the aperture of 0.2 mu m is arranged in the bottle cap.

The utility model discloses a further set up to: the upper end of the sealing cover is provided with a handle, and the water inlet end of the liquid inlet pipe extends out of the side edge of the handle.

To sum up, the utility model discloses following beneficial effect has:

before using the utility model, the utility model is sterilized, placed on a super clean bench and opened the sealing cover, the separated stem cells are respectively planted in the culture holes and added with a certain culture solution, each hole is planted with a stem cell, then the sealing cover is covered, the feed pipe is connected with a water pump, the water pump absorbs the culture solution, the nutrient solution flows slowly in the water channel along the feed pipe water inlet channel, and finally flows away from the liquid outlet pipe communicated with the tail end of the water channel and is recycled uniformly, in the process, when the new culture solution flows in the water channel, not only fresh nutrients are provided for the stem cells and metabolites of the cells are taken away, but also the living environment of the stem cells in the body can be better simulated, and of course, because the stem cells grow in suspension in the culture holes, the flowing speed of the nutrient solution cannot be too high so as to prevent the stem cells from flowing away along with the nutrient solution.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

fig. 2 is a plan view of the present invention (with the sealing cap removed);

fig. 3 is a cross-sectional view of the present invention.

In the figure: 1. a culture bottle; 11. a bottle body; 111. a bottle body; 112. a bottleneck; 12. a bottle cap; 13. a sealing cover; 131. an inner cover; 132. an outer cover; 133. a seal ring; 14. a fixing ring; 15. a handle; 2. a baffle plate; 3. a water channel; 4. a culture well; 5. a liquid inlet pipe; 6. a liquid outlet pipe; 7. and (5) filtering the membrane.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "left", "right", "front", "rear", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

Furthermore, the terms "horizontal", "vertical" and the like do not imply that the components are required to be absolutely horizontal or pendant, but rather may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present invention, it should also be noted that, unless explicitly stated or limited otherwise, the terms "disposed," "connected," and "connected" are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integrally connected; they may be mechanically coupled, directly coupled, indirectly coupled through intervening media, or may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

First embodiment, a blake bottle suitable for 3D cultivates stem cell, as shown in fig. 1 to fig. 3, including blake bottle 1, blake bottle 1 includes body 11 and bottle lid 12, body 11 includes bottle 111 and bottleneck 112, bottle 111 is the hollow form of cylinder and upper end opening, the open end of bottle 111 is equipped with the sealed lid 13 that can open, the one end of bottleneck 112 communicates in bottle 111, the one end that bottle 111 was kept away from to bottleneck 112 and bottle lid 12 threaded connection, body 11 and sealed lid 13 are transparent, in this embodiment, body 11 and sealed lid 13 are made by transparent plastics, of course, also can be glass, can not influence the protection scope of the utility model.

In detail, the culture flask 1 is easy to operate and has high safety factor when used for culturing cells; the sealing cover 13 can be repeatedly opened for a plurality of times, so that a user can conveniently operate in the bottle; the body 11 and the sealing cover 13 are transparent, so that the condition in the culture bottle 1 can be observed conveniently.

The center of the bottom of the bottle body 111 is provided with a baffle 2 distributed in a vortex line, the baffle 2 and the baffle form a water channel 3, the water channel 3 is also distributed in a vortex line, the bottom of the water channel 3 is provided with a plurality of culture holes 4 at equal intervals except the center of the water channel 3, the bottoms of the culture holes 4 are in a hemispherical cambered surface, and the cambered surface is an ultra-low adsorption surface; the sealing cover 13 is provided with a liquid inlet pipe 5 communicated with the inner cavity of the bottle body 111, the water outlet end of the liquid inlet pipe 5 is positioned in the bottle body 111 and aligned with the center of the water channel 3, and the water inlet end of the liquid inlet pipe 5 is positioned outside the bottle body 111; the circumference of the bottle body 111 is provided with a liquid outlet pipe 6 communicated with the inner cavity of the bottle body 111, the water inlet end of the liquid outlet pipe 6 is communicated with one end of the water channel 3 far away from the center, and the water outlet end of the liquid outlet pipe 6 is positioned outside the bottle body 111.

In detail, the culture solution flows into the center of the water channel 3 from the liquid inlet pipe 5, flows along the water channel 3 and passes through each culture hole 4, the normal growth of stem cells is not influenced under the condition that the flow rate of the culture solution is slow, the culture solution finally flows out from the liquid outlet pipe 6,

preferably, the opening end of the bottle body 111 is vertically provided with a fixing ring 14 facing the inner cavity of the bottle body 111, the sealing cover 13 comprises an inner cover 131, an outer cover 132 and a sealing ring 133, the outer side of the inner cover 131 is matched with the inner side of the fixing ring 14, the outer cover 132 is arranged at the upper end of the inner cover 131 and is erected at the opening end of the bottle body 111, and the sealing ring 133 is sleeved at the outer side of the inner cover 131 and forms a seal with the fixing ring. Specifically, when the sealing cap 13 is fitted to the open end of the bottle body 111, the sealing ring 133 seals the gap between the fixing ring 14 and the inner cap 131, and the lower edge of the outer cap 132 is engaged with the upper end of the open end of the bottle body 111, thereby preventing the sealing cap 13 from falling into the bottle body 111.

Preferably, the open end of the body 111 is circular. Can correspond to the water channel 3, and obtain larger and more convenient operation space.

Preferably, the height difference between the liquid outlet end of the liquid inlet pipe 5 and the bottom of the bottle body 111 is 1 mm.

Preferably, the neck 112 is formed on the circumferential side of the body 111, and the bottom of the neck 112 forms an angle of 10 ° with the bottom of the body 111.

Preferably, the bottle cap 12 is a vented cap. Of course, the cover can be a filtering cover, which does not affect the protection scope of the present invention.

Preferably, the filter membrane 7 with the pore diameter of 0.2 μm is arranged in the bottle cap 12. Allowing gas to pass through, and microorganisms such as bacteria cannot pass through.

Preferably, the upper end of the sealing cover 13 is provided with a handle 15, and the liquid inlet end of the liquid inlet pipe 5 extends out of the side edge of the handle 15. Facilitating the opening of the sealing lid 13.

Example two: as shown in fig. 1 to fig. 3, the same features of the first embodiment are not repeated, and the different features of the first embodiment are as follows:

in this embodiment, the height difference between the liquid outlet end of the liquid inlet pipe 5 and the bottom of the bottle 111 is 3 mm.

The bottom surface of the bottle neck 112 and the bottom surface of the bottle body 111 form an included angle of 15 ° in this embodiment.

Example three: as shown in fig. 1 to fig. 3, the same features of the first embodiment are not repeated, and the different features of the first embodiment are as follows:

in this embodiment, the height difference between the liquid outlet end of the liquid inlet pipe 5 and the bottom of the bottle 111 is 5 mm.

The bottom surface of the bottle neck 112 and the bottom surface of the bottle body 111 form an included angle of 30 ° in this embodiment.

It should be noted that, the height difference between the liquid outlet end of the liquid inlet pipe 5 and the bottom of the bottle 111 of the present invention is not only limited to the first embodiment, the second embodiment and the third embodiment, but also can be any value within the range of 1-5mm, which does not affect the protection scope of the present invention, the height difference between the liquid outlet end of the liquid inlet pipe 5 and the bottom of the bottle 111 is too large, which will cause the culture solution to splash around, and affect the flow rate, and the height difference is too small, which will cause the nutrient solution to flow unsmoothly, therefore, the height difference between the liquid outlet end of the liquid inlet pipe 5 and the bottom of the bottle 111 is suitable within 1-5 mm.

It should be noted that the included angle formed between the bottom surface of the bottle neck 112 and the bottom surface of the bottle body 111 is not limited to the first embodiment, the second embodiment and the third embodiment, and may be any value within the range of 10-30 °, which does not affect the protection scope of the present invention.

The using method comprises the following steps: before using the utility model, the utility model is sterilized, placed on a super clean bench and the sealing cover 13 is opened, the separated stem cells are respectively planted in the culture holes 4 and added with certain culture solution, each hole is planted with a stem cell, the sealing cover 13 is covered, the liquid feeding pipe 5 is connected with a water pump, the water pump absorbs the culture solution and flows into the water inlet channel 3 along the liquid feeding pipe 5, the nutrient solution slowly flows in the water channel 3 and finally flows away from the liquid outlet pipe 6 communicated with the tail end of the water channel 3 to be recycled and processed uniformly, in the process, when the new culture solution flows in the water channel 3, not only fresh nutrients are provided for the stem cells and metabolites of the cells are taken away, but also the living environment of the stem cells in the body can be better simulated, and of course, since the stem cells grow in suspension in the culture wells 4, the flow rate of the nutrient solution cannot be too high, so that the stem cells cannot flow away with the nutrient solution.

The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications to the present embodiment without inventive contribution as required after reading the present specification, but all of them are protected by patent laws within the scope of the claims of the present invention.

Claims

1. A culture flask suitable for 3D culture of stem cells, comprising a culture flask (1), characterized in that: the culture bottle (1) comprises a bottle body (11) and a bottle cap (12), the bottle body (11) comprises a bottle body (111) and a bottle neck (112), the bottle body (111) is cylindrical and hollow, the upper end of the bottle body is open, an openable sealing cap (13) is arranged at the open end of the bottle body (111), one end of the bottle neck (112) is communicated with the bottle body (111), one end, far away from the bottle body (111), of the bottle neck (112) is in threaded connection with the bottle cap (12), and the bottle body (11) and the sealing cap (13) are both transparent;

the center of the bottom of the bottle body (111) is provided with a baffle (2) which is distributed in a vortex line shape, the baffle (2) and the baffle form a water channel (3), the bottom of the water channel (3) is provided with a plurality of culture holes (4) at equal intervals except the center of the water channel (3), the bottoms of the culture holes (4) are hemispherical cambered surfaces, and the cambered surfaces are ultra-low adsorption surfaces; the sealing cover (13) is provided with a liquid inlet pipe (5) communicated with the inner cavity of the bottle body (111), the liquid outlet end of the liquid inlet pipe (5) is positioned in the bottle body (111) and aligned with the center of the water channel (3), and the liquid inlet end of the liquid inlet pipe (5) is positioned outside the bottle body (111); the bottle is characterized in that a liquid outlet pipe (6) communicated with the inner cavity of the bottle body (111) is arranged on the circumferential side of the bottle body (111), the water inlet end of the liquid outlet pipe (6) is communicated with one end, far away from the center, of the water channel (3), and the water outlet end of the liquid outlet pipe (6) is located on the outer side of the bottle body (111).

2. A culture flask suitable for 3D culture of stem cells according to claim 1, wherein: the vertical solid fixed ring (14) towards bottle (111) inner chamber that is provided with of open end of bottle (111), sealed lid (13) include inner cup (131), enclosing cover (132) and sealing washer (133), the outside of inner cup (131) with the inboard phase-match of solid fixed ring (14), enclosing cover (132) are located the upper end of inner cup (131) is set up the open end of bottle (111), sealing washer (133) cover is established the inner cup (131) outside and with gu fixed ring (14) forms sealedly.

3. A culture flask suitable for 3D culture of stem cells according to claim 2, wherein: the open end of the bottle body (111) is circular.

4. A culture flask suitable for 3D culture of stem cells according to claim 1, wherein: the height difference between the liquid outlet end of the liquid inlet pipe (5) and the bottom of the bottle body (111) is 1-5 mm.

5. A culture flask suitable for 3D culture of stem cells according to claim 1, wherein: the bottle neck (112) is arranged on the circumferential side of the bottle body (111), and the included angle formed by the bottom surface of the bottle neck (112) and the bottom surface of the bottle body (111) is 10-30 degrees.

6. A culture flask suitable for 3D culture of stem cells according to claim 1, wherein: the bottle cap (12) is an air hole type cap.

7. A culture flask suitable for 3D culture of stem cells according to claim 1, wherein: a filter membrane (7) with the aperture of 0.2 mu m is arranged in the bottle cap (12).

8. A culture flask suitable for 3D culture of stem cells according to claim 1, wherein: the upper end of the sealing cover (13) is provided with a handle (15), and the water inlet end of the liquid inlet pipe (5) extends out of the side edge of the handle (15).