CN213835319U

CN213835319U - Efficient cell culture device takes a sample

Info

Publication number: CN213835319U
Application number: CN202022806381.6U
Authority: CN
Inventors: 陈艳
Original assignee: Nanjing Zoonbio Biotechnology Co ltd
Current assignee: Nanjing Zoonbio Biotechnology Co ltd
Priority date: 2020-11-27
Filing date: 2020-11-27
Publication date: 2021-07-30
Anticipated expiration: 2030-11-27

Abstract

The utility model discloses a cell culture device with high sampling efficiency, which comprises a shaking bottle and a base, wherein the shaking bottle is arranged on the base; the top of the shake flask is provided with a threaded opening, the inner wall of the threaded opening is in threaded connection with a knob, and the top of the knob is fixedly connected with a breathable bottle cap; the ventilating bottle cap is provided with a through hole, one end of the hose is an injector connecting port, and the other end of the hose is attached to the bottom of the inner wall of the shake flask after penetrating through the through hole. The cell culture device with high sampling efficiency can be matched with an injector by arranging the hose for communicating the inside and the outside of the shake flask, can be used for quickly sampling cells and conveniently supplementing a fresh culture medium, so that the cells in the shake flask can be quickly sampled, and meanwhile, the inside of the shake flask is not easily polluted; meanwhile, the efficiency of extracting the cells in the shake flask can be improved, and the problems that the growth state of the cells in the shake flask cannot be well monitored when the cells grow in a suspension state and the efficiency is low during sampling are solved.

Description

Efficient cell culture device takes a sample

Technical Field

The utility model belongs to the technical field of cell culture equipment, in particular to sample efficient cell culture device.

Background

Cell culture (cell culture) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions, and the like) in vitro to enable the in vivo environment to survive, grow, reproduce and maintain the main structure and function, and for the whole bioengineering technology and one of the biological cloning technologies, the cell culture technology is an important and common technology in the research method of cell biology, and a large number of cells can be obtained through cell culture, and the signal transduction, the anabolism of the cells, the growth and the reproduction of the cells and the like can be researched by the cell culture technology.

The in vitro culture of cells has become the indispensable content of life research and practice, the cultured cell types are various, from viruses to bacteria and fungi, from human cells to animal cells and plant cells, some cells and tissues can grow under the suspension state, but because the cell suspension domestication process needs to constantly monitor the growth state, the sampling and counting are carried out constantly in the process, the sampling efficiency is low, secondly, the cells are easy to agglomerate in the cell culture process, thus the survival rate of the cells can be influenced, the counting result of the cells is also influenced, the problems of high culture and detection difficulty are solved, and therefore structural innovation is needed to solve the specific problems.

SUMMERY OF THE UTILITY MODEL

The main objective of the utility model is to provide a sample efficient cell culture device, its efficiency that can improve when cell to shaking in the bottle draws, solves to shake the bottle in the cell grow under the suspended state, can not monitor its growth state well, problem that the efficiency is low simultaneously when taking a sample.

The utility model discloses a secondary purpose lies in providing a sample efficient cell culture device, can solve to shake the easy agglomeration of the cell in the bottle, influences the survival rate of cell, also influences the problem of the count result of cell simultaneously to reach the effect of shaking even dispersion to the cell in shaking the bottle.

In order to achieve the above purpose, the solution of the present invention is:

a cell culture device with high sampling efficiency comprises a shake flask and a base, wherein the shake flask is arranged on the base; the top of the shake flask is provided with a threaded opening, the inner wall of the threaded opening is in threaded connection with a knob, and the top of the knob is fixedly connected with a breathable bottle cap; the ventilating bottle cap is provided with a through hole, one end of the hose is an injector connecting port, and the other end of the hose is attached to the bottom of the inner wall of the shake flask after penetrating through the through hole.

The hose is provided with a pinching port at the part outside the shake flask and close to the connection port of the injector.

The inner wall of the shake flask is attached with a cell filter screen, and the shape of the shake flask is the same as that of the cell filter screen.

The top of the base is provided with a first groove, the bottom of the inner wall of the first groove is provided with a second groove, and the inner wall of the second groove is fixedly provided with a micro motor; the driving end of the micro motor is connected with the rotating seat, the top of the rotating seat is fixedly provided with a sucker, and the edge of the top of the rotating seat is fixedly connected with a baffle.

The diameter of the rotary seat is equal to that of the bottom of the shake flask, and the outer wall of the rotary seat is attached to the inner wall of the first groove.

The contact part of the breathable bottle cap and the shake flask is provided with a sealing strip.

After the scheme is adopted, the utility model discloses compare prior art's beneficial effect is:

(1) the utility model can be matched with the injector through arranging the hose for communicating the inside and the outside of the shake flask, and can be used for quickly sampling cells and conveniently supplementing fresh culture medium, thereby quickly sampling the cells in the shake flask and not easily polluting the inside of the shake flask;

(2) the utility model is provided with the nipping port at one end of the hose close to the joint of the injector, and when the sampling is not carried out, the nipping port can be closed to avoid the pollution to the inner wall of the hose;

(3) the cell filter screen is arranged in the shake flask, and the rotary seat is arranged at the bottom of the shake flask, so that when the cells are agglomerated, the micro motor drives the rotary seat to drive the shake flask to rotate, the cells in the shake flask are dispersed through the cell filter screen, the survival rate of the cells is improved, and the accuracy of cell counting results is improved;

(4) the utility model discloses a sucking disc that sets up can fix shaking the bottle, avoids shaking the bottle and takes place to drop shaking the in-process.

Drawings

Fig. 1 is a schematic perspective view of the present invention;

FIG. 2 is a cross-sectional view of FIG. 1;

fig. 3 is a schematic structural diagram of the base of the present invention.

Reference numerals:

1. an injector mouthpiece; 2. pinching the opening; 3. a hose;

4. a breathable bottle cap; 5. shaking the flask;

6. a threaded opening; 7. a knob;

8. a cell filter screen; 9. a first groove;

10. a baffle plate; 11. a suction cup; 12. a rotating base;

13. a micro motor; 14. a second groove; 15. a base; 16. and a through hole.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.

As shown in fig. 1 and 2, the utility model provides a sample efficient cell culture device, including shaking bottle 5, shake bottle 5's top and be provided with screw thread mouth 6, the inner wall threaded connection of screw thread mouth 6 has knob 7, the ventilative bottle lid 4 of top fixedly connected with of knob 7, and through-hole 16 has been seted up at the top of ventilative bottle lid 4, and the inner wall fixedly connected with hose 3 of through-hole 16 shakes the bottom of bottle 5 and is provided with base 15.

In the embodiment, the top of the hose 3 is provided with an injector connecting port 1, the outer wall of the hose 3 is provided with a nipping port 2, and the bottom of the hose 3 is attached to the bottom of the inner wall of the shake flask 5; a sealing strip is arranged between the bottom of the breathable bottle cap 4 and the top of the shake flask 5, and one end of the hose 3 is arranged above the breathable bottle cap 4; through the hose 3 that sets up, make hose 3 can be used for the cell sample fast and conveniently supplement fresh culture medium to can be fast to shaking the cell in the bottle 5 and take a sample, be difficult for simultaneously shaking 5 insides and cause the pollution, in addition, the one end of hose 3 is provided with pinches a mouthful 2, when not taking a sample, can avoid 3 inner walls of hose to receive the pollution through closing pinches a mouthful 2.

As a preferred embodiment of the present invention, as shown in fig. 2, the inner wall of the shake flask 5 is attached to the outer wall of the cell filter screen 8, and the shape of the shake flask 5 is the same as the shape of the cell filter screen 8; meanwhile, as shown in fig. 3, a first groove 9 is formed in the top of the base 15, a second groove 14 is formed in the bottom of the inner wall of the first groove 9, a micro motor 13 is fixedly installed on the inner wall of the second groove 14, a rotating base 12 is fixedly connected to the top of the micro motor 13, the diameter of the rotating base 12 is equal to that of the bottom of the shake flask 5, the outer wall of the rotating base 12 is attached to the inner wall of the first groove 9, a suction cup 11 is fixedly installed on the top of the rotating base 12, and a baffle 10 is fixedly connected to the edge of the top of the rotating base 12; through the arranged cell culture filter screen 8, when cells are agglomerated, the rotation of the micro motor 13 enables the rotating seat 12 at the top of the driving end of the micro motor 13 to drive the shake flask 5 to rotate, so that the cells in the shake flask 5 are dispersed through the cell filter screen 8, the survival rate of the cells is improved, and meanwhile, the accuracy of cell counting results is also improved; the sucking disc 11 provided in this embodiment can fix the shake flask 5, so as to prevent the shake flask 5 from falling off during shaking.

The utility model discloses a theory of operation is: as shown in fig. 1-3, when cell sampling or fresh culture medium supplement is required, cells in the shake flask 5 can be rapidly sampled through the syringe adaptor 1 on the hose 3, and the interior of the shake flask 5 is not easily polluted; when the sampling is not carried out, the nipping port 2 at one end of the hose 3 can be closed, so that the inner wall of the hose 3 is prevented from being polluted; in addition, when the cells are agglomerated, the micro motor 13 is started to drive the rotating seat 12 to drive the shake flask 5 to rotate, so that the cells in the shake flask 5 are dispersed through the cell filter screen 8, the survival rate of the cells is improved, and the accuracy of a cell counting result is improved; the shake flask 5 can be fixed by the arranged sucking disc 11, the shake flask 5 is prevented from falling off in the shaking process,

in the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description of the present invention and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above should not be understood to necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by those skilled in the art.

Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.

Claims

1. A cell culture device with high sampling efficiency is characterized in that: comprises a shake flask and a base, wherein the shake flask is arranged on the base; the top of the shake flask is provided with a threaded opening, the inner wall of the threaded opening is in threaded connection with a knob, and the top of the knob is fixedly connected with a breathable bottle cap; the ventilating bottle cap is provided with a through hole, one end of the hose is an injector connecting port, and the other end of the hose is attached to the bottom of the inner wall of the shake flask after penetrating through the through hole.

2. A high efficiency sampling cell culture assembly as recited in claim 1 wherein: the hose is provided with a pinching port at a position close to the connection port of the injector.

3. A high efficiency sampling cell culture assembly as recited in claim 1 wherein: the inner wall of the shake flask is attached with a cell filter screen, and the shape of the shake flask is the same as that of the cell filter screen.

4. A high efficiency sampling cell culture assembly as claimed in claim 3 wherein: the top of the base is provided with a first groove, the bottom of the inner wall of the first groove is provided with a second groove, and the inner wall of the second groove is fixedly provided with a micro motor; the driving end of the micro motor is connected with the rotating seat, the top of the rotating seat is fixedly provided with a sucker, and the edge of the top of the rotating seat is fixedly connected with a baffle.

5. A high efficiency sampling cell culture assembly as claimed in claim 4 wherein: the diameter of the rotating seat is equal to that of the bottom of the shake flask, and the outer wall of the rotating seat is attached to the inner wall of the first groove.

6. A high efficiency sampling cell culture assembly as recited in claim 1 wherein: and a sealing strip is arranged at the contact part of the breathable bottle cap and the shake flask.