CN205576165U - Modified cell culture bottle - Google Patents
Modified cell culture bottle Download PDFInfo
- Publication number
- CN205576165U CN205576165U CN201620271905.7U CN201620271905U CN205576165U CN 205576165 U CN205576165 U CN 205576165U CN 201620271905 U CN201620271905 U CN 201620271905U CN 205576165 U CN205576165 U CN 205576165U
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- CN
- China
- Prior art keywords
- bottle
- observation portion
- observation part
- bottleneck
- cell
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004113 cell culture Methods 0.000 title abstract description 10
- 239000012528 membrane Substances 0.000 claims abstract description 33
- 235000012771 pancakes Nutrition 0.000 claims abstract description 10
- 239000012930 cell culture fluid Substances 0.000 claims description 25
- 230000006872 improvement Effects 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 4
- 229920002379 silicone rubber Polymers 0.000 claims description 3
- 239000004945 silicone rubber Substances 0.000 claims description 3
- 239000002861 polymer material Substances 0.000 claims 1
- 230000019522 cellular metabolic process Effects 0.000 abstract description 5
- 238000007689 inspection Methods 0.000 abstract description 4
- 238000001914 filtration Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 11
- 230000000694 effects Effects 0.000 description 7
- 230000010261 cell growth Effects 0.000 description 5
- 239000012531 culture fluid Substances 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 230000005484 gravity Effects 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 230000003833 cell viability Effects 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229950000845 politef Drugs 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a modified cell culture bottle, the blake bottle includes bottle, bottle lid and observation part, one side of bottle sets up transparent observation part, and this observation part's shape is hollow pancake cuboid, and this observation part's bottom face and the bottom surface of bottle flush the setting mutually, and this observation part is through settings that be linked together in check valve and the bottle, and this observation part's top and observation part be inside to be linked together to make has a bleeder vent, and the top of this bleeder vent closely sets up filtration membrane, the bottom is linked together and sets up a drain pipe in observation part's lateral wall and the observation part, sets up an ooff valve on this drain pipe. The truly circumstances of the internal cell culture liquid of reaction bulb of testing result is guaranteed to this blake bottle, guarantees the accuracy of testing result, guarantees the exactness of results such as scientific research research, waits the operation to bring the facility for inspection, also guarantees cell culture's etc. Accuracy, and the cell itself after the assurance cultivation completion or the correct operation of cell metabolism result can guarantee the result of use.
Description
Technical field
This utility model belongs to cytobiology equipment technical field, the Tissue Culture Flask of a kind of improvement.
Background technology
Cell is cultivated and is referred under conditions of manually providing, the growth that cell is carried out in vitro, and in incubation, the cell of growth is no longer divided into tissue, is the basis in biotechnology and the important means of production.Its purpose is to harvesting itself or obtain products of cellular metabolism.
nullTissue Culture Flask is that the growth of cell provides necessary environment，The kind of Tissue Culture Flask is the most more at present，Function is the most more，As patent publication No. CN202369595U provides a kind of culture bottle having cell cultivation and observing double-purpose functional，It includes horizontal segment，The sidewall of bottle is provided with horizontal segment and the two is formed integrally，During use, the horizontal segment being shaped as pancake cuboid is placed under microscope，Can directly observe the cell cultivated，Without the culture fluid containing cell is taken out，It is positioned on microscope slide observation again，Efficiently avoid the culture fluid polluted in culture bottle，But，Owing to this horizontal segment is arranged at the side of bottle and for pancake cuboid，Cell is when cultivating，Poor fluidity due to cell culture fluid，And the gas etc. in bottle will not be distributed in bottle and horizontal segment sometimes equably，At this moment the cell culture fluid that will result in horizontal segment can produce different from the cell concentration etc. of the cell culture fluid in bottle，If now operator is by the cell in eye-level section and then the growing state etc. of the cell in inferring bottle，Testing result can not the situation of the most correctly cell in reaction bulb，Testing result can be caused inaccurate，It cannot be guaranteed that the correctness of the results such as scientific research，It is the most unfavorable to bring to operations such as inspections，Cannot guarantee that the accuracy of cell cultivation etc. simultaneously，It cannot be guaranteed that cell itself after having cultivated or products of cellular metabolism is proper use of，It cannot be guaranteed that using effect.
By retrieval, find patent publication us as relevant to present patent application in next chapter:
A kind of Tissue Culture Flask (CN202369595U), relate to a kind of cell culture vessel, to solve existing Tissue Culture Flask poor air permeability, it is unfavorable for that cell grows, and when using existing Tissue Culture Flask to observe the cell cultivated, needs to take out culture fluid to be placed on microscope slide, it is placed on basis of microscopic observation, easily pollute culture bottle and be contained within the culture fluid of cell, and troublesome poeration, the problem adding the work difficulty of experimenter.It includes bottle and bottle cap, it also includes horizontal segment and semipermeable membrane, the sidewall of described bottle is provided with horizontal segment and the two is formed integrally, and the bottom face of horizontal segment flushes with the bottom surface of bottle, described horizontal segment to be shaped as pancake rectangular-shaped, bottle is made up of main body, bottleneck and bottleneck, the upper end of main body is disposed with bottleneck and bottleneck from the bottom to top, semipermeable membrane covers on the bottleneck of bottle, semipermeable membrane is compressed by the bottle cap being connected with the bottleneck of bottle, and the top of bottle cap is provided with at least one passage.This utility model is cultivated for cell.
By contrast, there are the different of essence from above-mentioned patent publication us in present patent application.
Utility model content
In place of the purpose of this utility model is to overcome the deficiencies in the prior art, it is provided that a kind of simple in construction, easy to use, it is ensured that the Tissue Culture Flask of the improvement of the correctness of testing result.
This utility model realizes by the following technical solutions:
nullA kind of Tissue Culture Flask of improvement，Described culture bottle includes bottle、Bottle cap and observation portion，The top of described bottle coaxially detachably closely arranges bottle cap，The side of described bottle arranges transparent observation portion，The pancake cuboid being shaped as hollow in this observation portion，The bottom face in this observation portion and the bottom surface of bottle flush setting，This observation portion passes through check valve and the setting that is connected in bottle，The top in this observation portion is shaped with an air-vent with being connected inside observation portion，The tight of this air-vent arranges filter membrane，This filter membrane is ventilative and external microbe can be stoped to enter inside observation portion，The sidewall in described observation portion is connected with observation portion inner bottom part and arranges a drain pipe，One switch valve is set on this drain pipe，Cell culture fluid within observation portion aseptically can be discharged or sucking-off by this drain pipe，Opening and closing of this drain pipe can be controlled by switch valve.
And, described culture bottle also includes semipermeable membrane, described semipermeable membrane is ventilative and external microbe can be stoped to enter in bottle, described bottle includes main body, bottleneck and bottleneck, the upper end of main body is disposed with bottleneck and bottleneck from the bottom to top, described semipermeable membrane covers on the bottleneck of bottle, and semipermeable membrane is compressed by the bottle cap arranged that removably connects with the bottleneck of bottle, and the top of bottle cap is provided with at least one bottle cap passage.
And, described semipermeable membrane uses medical macromolecular materials to make.
And, described medical macromolecular materials are politef or silicone rubber.
Advantage of the present utility model and good effect be:
null1、This Tissue Culture Flask is provided with observation portion，Operator in use can be by the growing state etc. of this transparent observation portion observation of cell，In use，When needs carry out observation of cell，The observation portion being shaped as pancake cuboid is placed in basis of microscopic observation，Operator elder generation closing switch valve，Open check valve again，Under gravity，Cell culture fluid in bottle can flow to inside observation portion，When cell culture fluid amount to outflow meets detection demand，Then close check valve，Then can observe、The operations such as detection，After observing，Open switch valve，Under sterile conditions the cell culture fluid within observation portion can be evacuated by external setting，I.e. it is ready for observation detection next time，Setting due to air-vent，The operation flowing into or discharging making this cell culture fluid within observation portion is the most smooth and easy，Improve work efficiency，The setting in this observation portion，Will not cell culture fluid in pollution bottle body when not only making detection，And can guarantee that detected cell culture fluid is exactly the cell culture fluid in bottle，Testing result can the situation of cell culture fluid in actual response bottle，Ensure that the accuracy of testing result，Ensure that the correctness of the results such as scientific research，Facility is brought to operations such as inspections，Also ensure that the accuracy of cell cultivation etc. simultaneously，Ensure the cell itself after having cultivated or products of cellular metabolism proper use of，Ensure that using effect.
2, this culture bottle is provided with semipermeable membrane, and semipermeable membrane is breathed freely, and Bottle cap top is provided with bottle cap passage, be conducive to cell ventilation, meeting the demand of oxygen and carbon dioxide, beneficially cell growth, cell proliferation is fast, culture effect is good, meanwhile, this semipermeable membrane can effectively stop external microbe to enter in bottle so that pollution-free in bottle, making the growth of cell, breed at one's best, cell viability is high.
3, this Tissue Culture Flask is applicable to the cultivation of scientific research cell, and school's biology curriculum experiment cell is cultivated, and is also applied for clinical medicine experiment cell and cultivates.
Accompanying drawing explanation
Fig. 1 is structure connection diagram of the present utility model；
Fig. 2 is the attachment structure partial sectional view of the bottleneck of bottle cap and bottle in Fig. 1.
Detailed description of the invention
For content of the present utility model, feature and effect can be further appreciated that, hereby enumerate following example, and it is as follows to coordinate accompanying drawing to describe in detail.It should be noted that the present embodiment is illustrative, it not determinate, it is impossible to thus limit protection domain of the present utility model.
nullA kind of Tissue Culture Flask of improvement，As depicted in figs. 1 and 2，Described culture bottle includes bottle 5、Bottle cap 1 and observation portion 9，The top of described bottle coaxially detachably closely arranges bottle cap，The side of described bottle arranges transparent observation portion，The pancake cuboid being shaped as hollow in this observation portion，The bottom face in this observation portion and the bottom surface of bottle flush setting，This observation portion passes through check valve 6 and the setting that is connected in bottle，The top in this observation portion is shaped with an air-vent 7 with being connected inside observation portion，The tight of this air-vent arranges filter membrane 8，This filter membrane is ventilative and external microbe can be stoped to enter inside observation portion，The sidewall in described observation portion is connected with observation portion inner bottom part and arranges a drain pipe 11，One switch valve 10 is set on this drain pipe，Cell culture fluid within observation portion aseptically can be discharged or sucking-off by this drain pipe，Opening and closing of this drain pipe can be controlled by switch valve.
nullThis Tissue Culture Flask is provided with observation portion，Operator in use can be by the growing state etc. of this transparent observation portion observation of cell，In use，When needs carry out observation of cell，The observation portion being shaped as pancake cuboid is placed in basis of microscopic observation，Operator elder generation closing switch valve，Open check valve again，Under gravity，Cell culture fluid in bottle can flow to inside observation portion，When cell culture fluid amount to outflow meets detection demand，Then close check valve，Then can observe、The operations such as detection，After observing，Open switch valve，Under sterile conditions the cell culture fluid within observation portion can be evacuated by external setting，I.e. it is ready for observation detection next time，Setting due to air-vent，The operation flowing into or discharging making this cell culture fluid within observation portion is the most smooth and easy，Improve work efficiency，The setting in this observation portion，Will not cell culture fluid in pollution bottle body when not only making detection，And can guarantee that detected cell culture fluid is exactly the cell culture fluid in bottle，Testing result can the situation of cell culture fluid in actual response bottle，Ensure that the accuracy of testing result，Ensure that the correctness of the results such as scientific research，Facility is brought to operations such as inspections，Also ensure that the accuracy of cell cultivation etc. simultaneously，Ensure the cell itself after having cultivated or products of cellular metabolism proper use of，Ensure that using effect.
In the present embodiment, described culture bottle also includes semipermeable membrane 12, described semipermeable membrane is ventilative and external microbe can be stoped to enter in bottle, described semipermeable membrane can use the medical macromolecular materials such as politef or silicone rubber to make, described bottle includes main body 4, bottleneck 3 and bottleneck 13, the upper end of main body is disposed with bottleneck and bottleneck from the bottom to top, described semipermeable membrane covers on the bottleneck of bottle, semipermeable membrane is compressed by the bottle cap arranged that removably connects with the bottleneck of bottle, and the top of bottle cap is provided with at least one bottle cap passage 2.
This culture bottle is provided with semipermeable membrane, and semipermeable membrane is breathed freely, and Bottle cap top is provided with bottle cap passage, be conducive to cell ventilation, meeting the demand of oxygen and carbon dioxide, beneficially cell growth, cell proliferation is fast, culture effect is good, meanwhile, this semipermeable membrane can effectively stop external microbe to enter in bottle so that pollution-free in bottle, making the growth of cell, breed at one's best, cell viability is high.
The using method of the Tissue Culture Flask of this improvement is as follows:
Being added by culture fluid in culture bottle, covered on bottleneck by semipermeable membrane, semipermeable membrane is compressed by the bottle cap being connected with the bottleneck of bottle, forms one and have ventilative cell culture environment in culture bottle；
When incubation needs observation of cell, when needs carry out observation of cell, the observation portion being shaped as pancake cuboid is placed in basis of microscopic observation, operator elder generation closing switch valve, open check valve again, under gravity, cell culture fluid in bottle can flow to inside observation portion, when cell culture fluid amount to outflow meets detection demand, then close check valve, then can observe, the operations such as detection, after observing, open switch valve, under sterile conditions the cell culture fluid within observation portion can be evacuated by external setting, i.e. it is ready for observation detection next time.
Claims (4)
1. the Tissue Culture Flask improved, described culture bottle includes bottle, bottle cap and observation portion, and the top of described bottle is coaxial
Detachably bottle cap is closely set, it is characterised in that: the side of described bottle arranges transparent observation portion, and this observation portion is shaped as
The pancake cuboid of hollow, the bottom face in this observation portion and the bottom surface of bottle flush settings, this observation portion by check valve and
Be connected in bottle setting, and the top in this observation portion is shaped with an air-vent with being connected inside observation portion, and the top of this air-vent is tight
Close arranging filter membrane, this filter membrane is ventilative and external microbe can be stoped to enter inside observation portion, the sidewall in described observation portion with
Observation portion inner bottom part is connected and arranges a drain pipe, and this drain pipe arranges a switch valve, can will observe portion by this drain pipe
Internal cell culture fluid is aseptically discharged or sucking-off, can control opening and closing of this drain pipe by switch valve.
The Tissue Culture Flask of improvement the most according to claim 1, it is characterised in that: described culture bottle also includes semipermeable membrane,
Described semipermeable membrane is ventilative and external microbe can be stoped to enter in bottle, and described bottle includes main body, bottleneck and bottleneck, main body
Upper end be disposed with bottleneck and bottleneck from the bottom to top, described semipermeable membrane covers on the bottleneck of bottle, semipermeable membrane by with bottle
The bottleneck of body removably connect arrange bottle cap compress, the top of bottle cap is provided with at least one bottle cap passage.
The Tissue Culture Flask of improvement the most according to claim 2, it is characterised in that: described semipermeable membrane uses medical high polymer
Material is made.
The Tissue Culture Flask of improvement the most according to claim 3, it is characterised in that: described medical macromolecular materials are poly-four
Fluorothene or silicone rubber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620271905.7U CN205576165U (en) | 2016-04-01 | 2016-04-01 | Modified cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620271905.7U CN205576165U (en) | 2016-04-01 | 2016-04-01 | Modified cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205576165U true CN205576165U (en) | 2016-09-14 |
Family
ID=56864170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620271905.7U Expired - Fee Related CN205576165U (en) | 2016-04-01 | 2016-04-01 | Modified cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205576165U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107236670A (en) * | 2017-07-28 | 2017-10-10 | 贵州阳润农业科技发展有限公司 | A kind of dendrobium candidum Tissue Culture Flask |
-
2016
- 2016-04-01 CN CN201620271905.7U patent/CN205576165U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107236670A (en) * | 2017-07-28 | 2017-10-10 | 贵州阳润农业科技发展有限公司 | A kind of dendrobium candidum Tissue Culture Flask |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160914 Termination date: 20210401 |