CN211394502U - Culture apparatus for cell experiments - Google Patents
Culture apparatus for cell experiments Download PDFInfo
- Publication number
- CN211394502U CN211394502U CN201922267605.8U CN201922267605U CN211394502U CN 211394502 U CN211394502 U CN 211394502U CN 201922267605 U CN201922267605 U CN 201922267605U CN 211394502 U CN211394502 U CN 211394502U
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- CN
- China
- Prior art keywords
- culture
- box
- culture medium
- cell
- sponge piece
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- Expired - Fee Related
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- 239000001963 growth medium Substances 0.000 claims abstract description 84
- 238000004113 cell culture Methods 0.000 claims abstract description 44
- 238000007789 sealing Methods 0.000 claims abstract description 13
- 238000003780 insertion Methods 0.000 claims abstract 4
- 230000037431 insertion Effects 0.000 claims abstract 4
- 239000007788 liquid Substances 0.000 claims description 36
- 239000002699 waste material Substances 0.000 claims description 13
- 239000012530 fluid Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 17
- 239000012982 microporous membrane Substances 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000004663 cell proliferation Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a culture device for cell experiments, which comprises a culture medium box and cell culture boxes, wherein the interior of the culture medium box is provided with a culture medium storage tank, one side of the top of the culture medium box is provided with a culture medium feed inlet communicated with the culture medium storage tank, the culture medium feed inlet is provided with a feed inlet sealing cover, the feed inlet sealing cover is in threaded connection with the culture medium box, the other side of the top of the culture medium box is provided with culture box insertion ports at equal intervals, and the culture box insertion ports are communicated with the culture medium storage tank, only need to change the base fluid of culture medium box can, it is higher to change efficiency.
Description
Technical Field
The utility model relates to a cell culture device technical field specifically is a culture apparatus for cell experiments.
Background
In vitro culture of cells and tissues has become an indispensable matter for life research and practice, and plays an important role in regenerative medicine and disease treatment. The cell types in culture are numerous, from viruses to bacteria and fungi, from human cells to animal and plant cells. Cell culture flasks are equipment for culturing cells, and the safety of the cell culture flasks is quite high, so that the cell culture flasks are widely favored by researchers in the pharmaceutical industry and the medical equipment industry.
However, since the cell culture flask is large in size and can culture only one cell at a time, and only the cell culture flask is added to the control group to culture the cells, it is necessary to treat each cell culture flask when the medium solution is replaced, which is inefficient.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a culture apparatus for cell experiments to when changing culture medium liquid, need handle every cell culture bottle among the solution prior art, the lower problem of efficiency.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a culture apparatus for cell experiments, includes culture medium box and cell culture box, the culture medium holding tank has been seted up to the inside of culture medium box, the culture medium feed inlet that is linked together with the culture medium holding tank is seted up to top one side of culture medium box, culture medium feed inlet department is provided with the sealed lid of feed inlet, the sealed lid of feed inlet is threaded connection with the culture medium box, the culture box interface has been seted up to the top opposite side of culture medium box equidistantly, the culture box interface is linked together with the culture medium holding tank, the cell culture box inserts the inside of culture medium box via the culture box interface, be provided with the external screw thread on the external surface of cell culture box, the cell culture box is threaded connection with the culture medium box, the opposite side lower part of culture medium box is provided with the waste liquid discharge port.
Preferably, the cell culture box comprises culture box body, culture box lid, sponge piece and micropore diaphragm plate, and the bottom of culture box body is provided with the inlet, and the inboard of culture box body is provided with sponge piece fixed station, and the sponge piece sets up in the top of sponge piece fixed station, and the bottom of sponge piece passes sponge piece fixed station and extends to inlet department, and the micropore diaphragm plate is tiled at the top of sponge piece, and the top at culture box body is installed to the culture box lid.
Preferably, the sponge block fixing table and the culture box body are of an integrated structure.
Preferably, the sponge block is arranged into a T-shaped structure.
Preferably, the surface of the culture box cover is provided with an air vent penetrating through the culture box cover.
Preferably, the bottom surface of the culture medium storage tank is inclined towards one side of the waste liquid discharge port, an outlet sealing cover is installed at the waste liquid discharge port, and the outlet sealing cover is in threaded connection with the waste liquid discharge port.
Compared with the prior art, the beneficial effects of the utility model are that:
the utility model discloses a set up a plurality of cell culture boxes of equal size and less volume and conveniently carry out the contrast test, because a plurality of cell culture boxes all peg graft inside the culture medium box, all use the culture medium liquid inside the culture medium box when carrying out cell culture, when needing to change the culture medium liquid, only need to change the culture medium liquid of culture medium box can, change efficiency is higher, because cell culture box and culture medium box are threaded connection, so can change the depth that the cell culture box inserts the culture medium box through twisting the cell culture box, conveniently carry out corresponding adjustment according to the user demand, when operating, the cell inoculation is on the surface of micropore diaphragm plate, through the water absorption characteristic of sponge piece, carry the base liquid to the surface of micropore diaphragm plate, the cell absorbs the nutrient through the micropore on the micropore diaphragm plate, this base liquid conveying mode can effectively avoid cell proliferation to pollute the base liquid, and simultaneously, the consumption of the base liquid can be reduced.
Drawings
The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:
fig. 1 is a schematic view of the overall internal structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the cell culture cassette of the present invention;
fig. 3 is a schematic structural view of the sealing cover of the feed inlet of the present invention.
In the figure: 1. a culture medium box; 2. a culture medium feed port; 3. a feed port sealing cover; 4. a culture box interface; 5. an external thread; 6. a cell culture cassette; 7. a waste liquid discharge port; 8. an outlet sealing cover; 9. a culture medium storage tank; 10. a culture box body; 11. a ventilation hole; 12. a culture box cover; 13. a sponge block; 14. a liquid inlet; 15. a sponge block fixing table; 16. a microporous membrane plate.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined to clearly and completely describe the technical solutions of the embodiments of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1 of the drawings, a drawing, fig. 3, in the embodiment of the utility model, a culture apparatus for cell experiments, including culture medium box 1 and cell culture box 6, culture medium holding tank 9 has been seted up to the inside of culture medium box 1, culture medium feed inlet 2 that is linked together with culture medium holding tank 9 has been seted up to top one side of culture medium box 1, culture medium feed inlet 2 department is provided with the sealed lid 3 of feed inlet, the sealed lid 3 of feed inlet is threaded connection with culture medium box 1, culture box interface 4 has been seted up to the top opposite side of culture medium box 1 equidistant, culture box interface 4 is linked together with culture medium holding tank 9, cell culture box 6 inserts the inside of culture medium box 1 via culture box interface 4, be provided with external screw thread 5 on the external surface of cell culture box 6, cell culture box 6 is threaded connection with culture medium box 1, the opposite side lower part of culture medium box 1 is provided with waste liquid discharge port 7.
Please refer to fig. 2, the cell culture box 6 is composed of a culture box body 10, a culture box cover 12, a sponge block 13 and a microporous membrane plate 16, a liquid inlet 14 is arranged at the bottom of the culture box body 10, a sponge block fixing table 15 is arranged at the inner side of the culture box body 10, the sponge block 13 is arranged at the top of the sponge block fixing table 15, the bottom of the sponge block 13 penetrates through the sponge block fixing table 15 and extends to the liquid inlet 14, the microporous membrane plate 16 is tiled at the top of the sponge block 13, and the culture box cover 12 is arranged at the top of the culture box body 10.
Referring to fig. 2, the sponge block fixing platform 15 and the cultivation box body 10 are of an integral structure, the sponge block 13 is of a T-shaped structure, and the surface of the cultivation box cover 12 is provided with a ventilation hole 11 penetrating through the cultivation box cover 12.
Referring to fig. 1, the bottom surface of the culture medium storage tank 9 is inclined to the side of the waste liquid discharge port 7, an outlet sealing cover 8 is mounted at the waste liquid discharge port 7, and the outlet sealing cover 8 is in threaded connection with the waste liquid discharge port 7; the cell culture boxes 6 with the same size and smaller volume are arranged to conveniently carry out a contrast test, as the cell culture boxes 6 are all inserted inside the culture medium box 1 and the culture medium liquid inside the culture medium box 1 is used during cell culture, only the culture medium liquid of the culture medium box 1 needs to be replaced when the culture medium liquid needs to be replaced, the replacement efficiency is higher, as the cell culture boxes 6 are in threaded connection with the culture medium box 1, the depth of the cell culture boxes 6 inserted into the culture medium box 1 can be changed by screwing the cell culture boxes 6, corresponding adjustment can be conveniently carried out according to the use requirement, when in operation, cells are inoculated on the surface of the microporous membrane plate 16, the culture medium liquid is conveyed to the surface of the microporous membrane plate 16 through the water absorption characteristic of the sponge block 13, the cells are taken in through micropores on the microporous membrane plate 16, the conveying mode of the culture medium can effectively avoid cell proliferation to pollute the culture medium, and simultaneously, the consumption of the base liquid can be reduced.
The utility model discloses a theory of operation and use flow: the utility model discloses a set up a plurality of equal size and less cell culture box 6 and conveniently carry out the contrast test, because a plurality of cell culture box 6 all peg graft in culture medium box 1 inside, all use the culture medium liquid in culture medium box 1 when carrying out cell culture, therefore when needing to change culture medium liquid, only need to change the culture medium liquid of culture medium box 1 can, it is higher to change efficiency, because cell culture box 6 is threaded connection with culture medium box 1, therefore can change the depth that cell culture box 6 inserted culture medium box 1 through twisting cell culture box 6, conveniently carry out corresponding adjustment according to the user demand, during the operation, the cell is inoculated on the surface of micropore diaphragm plate 16, through the water absorption characteristic of sponge piece 13, carry the base liquid to the surface of micropore diaphragm plate 16, the cell absorbs the nutrient through the micropore on the micropore diaphragm plate 16, this base liquid transport mode can effectually avoid the cell proliferation to pollute the base liquid, and simultaneously, the consumption of the base liquid can be reduced.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A culture apparatus for cell experiments, which comprises a culture medium box (1) and a cell culture box (6), and is characterized in that: a culture medium storage groove (9) is arranged in the culture medium box (1), a culture medium feeding hole (2) communicated with the culture medium storage groove (9) is arranged on one side of the top of the culture medium box (1), a feed inlet sealing cover (3) is arranged at the position of the culture medium feed inlet (2), the feed inlet sealing cover (3) is in threaded connection with the culture medium box (1), the other side of the top of the culture medium box (1) is provided with culture box insertion ports (4) at equal intervals, the culture box interface (4) is communicated with the culture medium storage tank (9), the cell culture box (6) is inserted into the culture medium box (1) through the culture box insertion port (4), the external surface of the cell culture box (6) is provided with an external thread (5), the cell culture box (6) is in threaded connection with the culture medium box (1), and a waste liquid discharge port (7) is formed in the lower portion of the side face of the other side of the culture medium box (1).
2. The culture device for cell experiments according to claim 1, wherein: cell culture box (6) are by cultivateing box body (10), cultivate lid (12), sponge piece (13) and micropore diaphragm plate (16) are constituteed, the bottom of cultivateing box body (10) is provided with inlet (14), the inboard of cultivateing box body (10) is provided with sponge piece fixed station (15), sponge piece (13) set up in the top of sponge piece fixed station (15), and the bottom of sponge piece (13) passes sponge piece fixed station (15) and extends to inlet (14) department, micropore diaphragm plate (16) tiling is at the top of sponge piece (13), cultivate the top at cultivateing box body (10) in lid (12).
3. A culture apparatus for cell experiments according to claim 2, wherein: the sponge block fixing table (15) and the culture box body (10) are of an integrated structure.
4. A culture apparatus for cell experiments according to claim 2, wherein: the sponge block (13) is arranged into a T-shaped structure.
5. A culture apparatus for cell experiments according to claim 2, wherein: the surface of the culture box cover (12) is provided with an air vent (11) which penetrates through the culture box cover (12).
6. The culture device for cell experiments according to claim 1, wherein: the bottom surface of the culture medium storage tank (9) inclines to one side of the waste liquid discharge port (7), an outlet sealing cover (8) is installed at the waste liquid discharge port (7), and the outlet sealing cover (8) is in threaded connection with the waste liquid discharge port (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922267605.8U CN211394502U (en) | 2019-12-17 | 2019-12-17 | Culture apparatus for cell experiments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922267605.8U CN211394502U (en) | 2019-12-17 | 2019-12-17 | Culture apparatus for cell experiments |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211394502U true CN211394502U (en) | 2020-09-01 |
Family
ID=72222388
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922267605.8U Expired - Fee Related CN211394502U (en) | 2019-12-17 | 2019-12-17 | Culture apparatus for cell experiments |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211394502U (en) |
-
2019
- 2019-12-17 CN CN201922267605.8U patent/CN211394502U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200901 |
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| CF01 | Termination of patent right due to non-payment of annual fee |