CN110669668B - Carbon dioxide incubator - Google Patents

Carbon dioxide incubator Download PDF

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Publication number
CN110669668B
CN110669668B CN201911019188.3A CN201911019188A CN110669668B CN 110669668 B CN110669668 B CN 110669668B CN 201911019188 A CN201911019188 A CN 201911019188A CN 110669668 B CN110669668 B CN 110669668B
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carbon dioxide
inner cavity
control system
cavity
independent
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CN110669668A (en
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庞逢祥
杨超
张博韬
赵臻
张立明
温邵博
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Qinhuangdao Zhaojian Biotechnology Co ltd
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Qinhuangdao Zhaojian Biotechnology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/20Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor

Abstract

The invention relates to a carbon dioxide incubator, which comprises a case body, case wheels and a control panel, wherein the case body is divided into a plurality of independent cavities, and an outer case door is hinged outside each independent cavity; an inner cavity is arranged in each independent cavity; each side of the inner side of the partition plate of the inner cavity is covered with a heat insulation layer; each inner cavity is provided with a respective inner glass door and is provided with an independent temperature control system, a carbon dioxide concentration control system, a constant humidity control system and a sterilization and disinfection device. The beneficial effects of the invention are as follows: cultures under different conditions are cultivated in the same incubator, cross contamination among cells is avoided, equipment investment cost of a plurality of incubators is saved, and a heat dissipation function is further increased. In addition, the cell is not influenced by external environment fluctuation due to intermediate disinfection and sterilization operation in the culture process, the operation is simple, the cell culture is not interrupted, and the cell culture efficiency is improved.

Description

Carbon dioxide incubator
Technical Field
The invention relates to the field of biological incubators, in particular to a carbon dioxide incubator.
Background
The carbon dioxide incubator realizes in vitro culture of cells by simulating the formation of a growth environment similar to that in a living body in the incubator. Carbon dioxide incubator is used for makingThe stable CO in the box body is required to have stable temperature (37 ℃) when in use 2 Level (5%), constant pH (pH: 7.2-7.4), and relatively high relative saturation humidity (95%), and are widely used in cell, tissue culture and culture of certain specific microorganisms.
However, the conventional carbon dioxide incubator cannot simultaneously culture cells under different culture conditions due to uniform internal conditions. Therefore, when it is desired to culture cells under different conditions, a plurality of incubators are purchased, which is costly and occupies a large space. On the other hand, the heating effect of current carbon dioxide incubator is comparatively concentrated, leads to cultivateing regional temperature distribution inhomogeneous, has both been difficult to provide invariable environment for cell culture, also can influence temperature sensor's feedback simultaneously. In addition, the carbon dioxide incubator in the prior art has a heating function, and cell pollution is caused by opening the refrigerator door, and the incubator also has no heat dissipation function, so that when the internal devices of the incubator generate heat, the temperature in the incubator can be influenced, and the incubator is unstable. In addition, when the sterilization and disinfection operation is performed again in the culture process, the cultured cells need to be taken out, so that the cell culture is interrupted and is inevitably influenced by external environment fluctuation.
Disclosure of Invention
First, the technical problem to be solved
The invention provides a carbon dioxide incubator to solve the problems that in the prior art, a single carbon dioxide incubator is difficult to culture cells under different culture conditions simultaneously, fluctuation and pollution are caused to the cultured cells by re-sterilization and disinfection in the process of opening and closing a box door and in the culture period, the heating effect is uneven, and the independent carbon dioxide incubator does not have a heat dissipation function and further has a temperature floating effect.
(II) technical scheme
In order to achieve the above purpose, the main technical scheme adopted by the invention comprises the following steps:
the carbon dioxide incubator comprises a case body, case wheels and a control panel, wherein the case body is divided into two parts, the upper part is a regulating area, and the lower part is a culturing area; the culture area is divided into a plurality of independent cavities by a partition board;
the side edge of the independent cavity is hinged with an outer box door, an inner cavity is arranged in the independent cavity, the inner cavity and the independent cavity are arranged in a non-contact mode, and the side edge of the inner cavity is hinged with an inner glass door;
each inner cavity is provided with an independent temperature control system, a carbon dioxide concentration control system and a constant humidity control system; the temperature control system, the carbon dioxide concentration control system and the constant humidity control system are integrated in the regulation area, and a regulation area shell is covered outside the regulation area;
the temperature control system comprises copper pipes fixed on the outer sides of all the surfaces of the inner cavity;
a character-returning plate is arranged between the independent cavity and the inner cavity, and the character-returning plate and the inner glass door are positioned on the same plane; when the outer box door is in an open state, the independent cavity is still a closed zone due to the arrangement of the reverse-square-shaped plate.
The carbon dioxide concentration control system comprises a carbon dioxide pipe joint, a pressure reducing valve and a carbon dioxide conveying pipe; the carbon dioxide conveying pipe comprises a carbon dioxide conveying main pipe and a plurality of carbon dioxide conveying branch pipes;
the pressure reducing valve is arranged on the carbon dioxide conveying main pipe, one end of the carbon dioxide conveying branch pipe is connected with the carbon dioxide conveying main pipe, and the other end of the carbon dioxide conveying branch pipe is connected with the carbon dioxide throttle valve at the outer side of the rear wall surface of each inner cavity;
the pressure reducing valve is arranged in the regulating area shell, and a pressure regulating window is arranged at a position of the regulating area shell corresponding to the pressure reducing valve;
further, the carbon dioxide pipe joint is externally connected with a carbon dioxide source.
The constant humidity control system comprises a water filling port, an atomization generator and a mist conveying pipe in the middle of the top of the regulation area;
the mist conveying pipe comprises a mist conveying main pipe and a plurality of mist conveying branch pipes, one end of the atomization generator is connected with the water filling port, and the other end of the atomization generator is connected with the mist conveying main pipe; and a mist throttle valve is arranged below each carbon dioxide throttle valve, and the mist throttle valve is connected with the mist conveying branch pipe.
Each surface in the inner cavity is attached with a heat insulation layer; each surface of each inner cavity is provided with a plurality of long and narrow pores.
Further, a silicone rubber sealing strip is arranged between the inner glass door and the inner cavity, and between the outer box door and the independent cavity.
Further, copper pipes are also fixed on the inner side of each outer box door.
Further, an ultraviolet lamp is arranged on the top wall of each inner cavity.
Further, a ventilation fan is arranged on the rear wall surface of each inner cavity.
Further, two side walls of the inner cavity are provided with laminate support plates perpendicular to the bottom surface, and the laminate support plates are provided with a plurality of grooves in the horizontal direction.
Further, a sensor protection cover is arranged at the top of each inner cavity, and a carbon dioxide concentration sensor, a humidity sensor and a temperature sensor are arranged in the sensor protection cover.
Further, the copper pipe is communicated with heating liquid or steam.
(III) beneficial effects
The beneficial effects of the invention are as follows:
1. by arranging a plurality of inner cavities, cultures under different conditions are cultivated in the same incubator, cross contamination among cells is avoided, and equipment investment cost of a plurality of incubators is saved.
2. The culture can be transferred to an inner cavity with proper culture conditions in time, the cells cannot be influenced by external environment fluctuation due to intermediate disinfection and sterilization operation in the culture process, the cell culture cannot be interrupted, and the cell culture efficiency is improved.
3. The carbon dioxide concentration, temperature, humidity and disinfection operation of each inner cavity can be independently controlled, and the cost input is not increased.
4. Through the setting of returning the font board, realized the heat dissipation function that carbon dioxide incubator did not possess among the prior art, and easy operation only need open outer chamber door can realize, need not worry pollution problem.
5. Six-face heating can be realized in the culture area of inner cavity, makes to be heated more evenly, more quick.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a carbon dioxide incubator;
FIG. 2 is a schematic diagram of the overall structure of the carbon dioxide incubator;
FIG. 3 is a rear view of the carbon dioxide incubator;
FIG. 4 is a schematic view of the structure of the cavity in the carbon dioxide incubator;
FIG. 5 is a schematic view of the back side wall of the chamber in the carbon dioxide incubator.
[ reference numerals description ]
101: a control panel; 102: a control button; 103: an outer door; 104: an independent cavity;
201: a chassis housing; 202: a carbon dioxide pipe joint; 203: a pressure regulating window; 204: a pressure reducing valve; 205: a water filling port; 206: a box wheel; 207: a regulatory region envelope;
301: a glass inner door; 302: an inner cavity; 303: copper pipe; 304: an ultraviolet lamp; 305: a sensor protection cover; 306: a laminate; 307: a silicone rubber sealing strip; 308: a laminate support plate;
401: a ventilation fan; 402: a mist throttle valve; 403: a carbon dioxide throttle valve; 404: a mist delivery pipe; 405: a carbon dioxide delivery tube.
Detailed Description
The invention will be better explained by the following detailed description of the embodiments with reference to the drawings.
A carbon dioxide incubator, as shown in fig. 1 and 2, comprises a case, a control panel 101 and a case wheel 206, wherein the case is divided into two parts, the upper part is a regulating area, and the lower part is a culturing area; the box body is divided into a plurality of independent cavities 104 by a partition board, and the side edge of each independent cavity 104 is hinged with an outer box door 103; an inner cavity 302 is arranged in the independent cavity 104, the inner cavity 302 and the independent cavity 104 are arranged in a non-contact mode, and the side edge of the inner cavity 302 is hinged with an inner glass door 301;
each of the inner chambers 302 has an independent temperature control system, carbon dioxide concentration control system, constant humidity control system; the temperature control system, the carbon dioxide concentration control system and the constant humidity control system are integrated in the regulation area, and a regulation area shell 207 is covered outside the regulation area;
the temperature control system comprises copper tubes 303 fixed outside the respective faces of the inner cavity 302; the copper pipe 303 is filled with heating liquid or gas, and heat is supplied to the inner cavity through heat conduction of the copper pipe. The temperature sensor is arranged in the inner cavity 302, so that the temperature change in the cavity can be fed back in time, and a constant temperature environment is further provided.
Further, copper tubes 303 are also fixed to the inner side of the outer box door 103, thus achieving six-sided heating of the culture in the inner cavity 302. Compared with the prior art, six-face heating is carried out on the culture cavity, so that heat distribution is uniform and concentrated, the heating process is quicker and more efficient, and the problems that temperature distribution in a culture area is uneven, a sensor is not representative to temperature feedback and the like are avoided.
The carbon dioxide concentration control system comprises a carbon dioxide pipe joint, a pressure reducing valve and a carbon dioxide conveying pipe; the carbon dioxide conveying pipe comprises a carbon dioxide conveying main pipe and a plurality of carbon dioxide conveying branch pipes;
the pressure reducing valve 204 is arranged on the carbon dioxide conveying main pipe, one end of the carbon dioxide conveying branch pipe is connected with the carbon dioxide conveying main pipe, and the other end of the carbon dioxide conveying branch pipe is connected with the carbon dioxide throttle valve 403 on the outer side of the rear wall surface of each inner cavity 302;
the pressure reducing valve is arranged in the regulation area shell, and a pressure regulating window 203 is arranged at a position of the regulation area shell 207 corresponding to the pressure reducing valve 204; a carbon dioxide concentration sensor is arranged in the inner cavity 302;
further, the carbon dioxide pipe joint 202 is externally connected with a carbon dioxide source.
The carbon dioxide pressure reducing valve 204 reduces the carbon dioxide to a suitable operating pressure; after exiting the pressure relief valve 204, the carbon dioxide passes through a carbon dioxide delivery manifold and then through a carbon dioxide delivery manifold associated with each of the inner chambers 302 to the inner chambers 302. The carbon dioxide throttle valve 403 may control the circulation and interruption of carbon dioxide in each inner cavity 302, and is independent of each other, so as to realize independent control of carbon dioxide in each inner cavity 302. In addition, the carbon dioxide concentration in the culture zone can be monitored in real time by the carbon dioxide sensor in each inner cavity 302, and the constant state of the carbon dioxide concentration in the culture zone can be maintained.
The constant humidity control system comprises a water injection port 205, an atomization generator, a mist conveying pipe 404 and a humidity sensor in the middle of the top of the regulation area; the water injection port 205 is externally connected with a water source;
the mist conveying pipe 404 comprises a mist conveying main pipe and a plurality of mist conveying branch pipes, one end of the atomization generator is connected with the water filling port 205, and the other end of the atomization generator is connected with the mist conveying main pipe; a mist throttle valve 402 is disposed below each of the carbon dioxide throttle valves 403, and the mist throttle valve 402 is connected to the mist delivery branch pipe. The atomization generator turns water into mist, so that the requirements of culture conditions are met; the mist throttle 404 can control the replenishment and interruption of water vapor in each inner cavity separately according to different requirements, so as to maintain the humidity of one or a plurality of inner cavities 302 constant.
Further, an ultraviolet lamp 304 is disposed on a top wall of each of the inner chambers 302. In the process of cell or tissue culture, the culture area needs to be disinfected and sterilized again to prevent the external environment from polluting the culture. However, since the carbon dioxide incubator in the prior art has only one culture area, the culture is often required to be placed in the external environment when the re-sterilization operation is performed in the culture process. Thus, on one hand, the external environment is difficult to meet the culture conditions, the environment fluctuation is caused, and the culture is influenced; on the other hand, the external environment outside the incubator is highly likely to cause contamination of the culture.
In the present invention, the operator can perform the sterilization operation on any inner cavity 302 at any time without worrying about the influence of environmental fluctuation and external pollution during the cultivation. The operator only needs to transfer the cell tissue and the like being cultured into the other inner cavity with proper carbon dioxide, temperature and humidity and disinfection and sterilization, so that the culture is not interrupted, the culture is not polluted, and the quality of the culture can be improved.
A zigzag plate 105 is arranged between the independent cavity 104 and the inner cavity 302, and the zigzag plate 105 and the inner glass door 301 are positioned on the same plane; when the outer door 103 is opened, the independent cavity 104 is still a closed area due to the provision of the zigzag plate 105. The zigzag plate 105 can prevent foreign substances from entering the cell culture area to pollute the culture substances. When the temperature rises due to the fact that certain parts in the incubator are heated for a long time, the heat dissipation operation can be realized only by opening the outer refrigerator door 301, no external pollution is caused, and due to the six-face heating system, even if the outer refrigerator door 301 is opened, the heat dissipation cannot cause fluctuation to the environmental conditions of the incubator.
Each side of the inner cavity 302 is attached with a heat insulating layer to ensure heat concentration; each surface of each inner cavity is provided with a plurality of long and narrow pores, and the pores enable carbon dioxide and water vapor required in the cell culture process to permeate through the pores, so that the carbon dioxide, the water vapor and the like in the culture environment are distributed uniformly.
Further, a silicone rubber sealing strip is arranged between the inner glass door 301 and the inner cavity 302, and between the outer door 103 and the independent cavity 104.
Further, a sensor protection cover 305 is disposed on top of each inner cavity 302, and the carbon dioxide concentration sensor, the humidity sensor and the temperature sensor are disposed in the sensor protection cover 305.
Further, a ventilation fan 401 is disposed on the rear wall surface of each inner cavity, when the cells are cultured, the ventilation fan 401 is opened, air uniformly flows in the whole box body, and carbon dioxide, humidity, heat and the like are further driven to be uniformly distributed in the inner cavity 302, so that culture conditions are more ideal.
Further, two side walls of the inner cavity 302 are provided with laminate support plates 308 with vertical bottom surfaces, and the laminate support plates 308 are provided with a plurality of grooves in horizontal directions. When the number of cells or tissues to be cultured is large, a plurality of laminates 306 can be placed and clamped in the grooves of the laminate support plates 308, so that the number of cultured cells or tissues is increased, and the space utilization rate is improved.
The carbon dioxide incubator in the prior art is only suitable for culturing the same type of culture, and if different types of culture are placed in the unified incubator, cross contamination among cells can be caused. According to the invention, a plurality of inner cavities are arranged in one incubator, so that cultures of different types can be cultivated simultaneously, the investment cost of equipment is not required to be increased additionally, and meanwhile, the cross contamination is avoided and the cultivation efficiency is improved.
The above embodiments are only for explaining the present invention and do not limit the scope of protection of the present invention, and those skilled in the art make various variations or modifications within the scope of the claims, which fall within the essential content of the present invention.

Claims (9)

1. The carbon dioxide incubator comprises a case body, a case wheel (206) and a control panel (101), and is characterized in that the case body is divided into two parts, wherein the upper part is a regulating area, and the lower part is a culturing area; the culture area is divided into a plurality of independent cavities (104) by a partition board;
the side edge of the independent cavity (104) is hinged with the outer box door (103), an inner cavity (302) is arranged in the independent cavity (104), the inner cavity (302) and the independent cavity (104) are arranged in a non-contact mode, and the side edge of the inner cavity (302) is hinged with the inner glass door (301);
each inner cavity (302) is provided with an independent temperature control system, a carbon dioxide concentration control system and a constant humidity control system; the temperature control system, the carbon dioxide concentration control system and the constant humidity control system are integrated in the regulation area, and a regulation area shell (207) is covered outside the regulation area;
the temperature control system comprises copper pipes (303) fixed outside all surfaces of the inner cavity (302);
a back-shaped plate (105) is arranged between the independent cavity (104) and the inner cavity (302), and the back-shaped plate (105) and the inner glass door (301) are positioned on the same plane; when the outer box door (103) is in an open state, the independent cavity (104) is still a closed section due to the arrangement of the zigzag plate (105);
each surface of each inner cavity (302) is provided with a plurality of long and narrow pores;
a ventilation fan (401) is arranged on the rear wall surface of each inner cavity (302).
2. The carbon dioxide incubator of claim 1, wherein the carbon dioxide concentration control system comprises a carbon dioxide adapter (202), a pressure reducing valve (204), a carbon dioxide delivery tube (405); the carbon dioxide conveying pipe (405) comprises a carbon dioxide conveying main pipe and a plurality of carbon dioxide conveying branch pipes;
the pressure reducing valve (204) is arranged on the carbon dioxide conveying main pipe, one end of the carbon dioxide conveying branch pipe is connected with the carbon dioxide conveying main pipe, and the other end of the carbon dioxide conveying branch pipe is connected with the carbon dioxide throttle valve (403) on the outer side of the rear wall surface of each inner cavity (302);
the pressure reducing valve (204) is arranged in the regulating area shell (207), and a pressure regulating window (203) is arranged at a position of the regulating area shell corresponding to the pressure reducing valve (204);
the carbon dioxide pipe joint (202) is externally connected with a carbon dioxide source.
3. The carbon dioxide incubator of claim 1, wherein the constant humidity control system comprises a water injection port (205), an atomization generator, a mist delivery pipe (404) intermediate the top of the regulatory region;
the mist conveying pipe (404) comprises a mist conveying main pipe and a plurality of mist conveying branch pipes, one end of the atomization generator is connected with the water filling port, and the other end of the atomization generator is connected with the mist conveying main pipe; a mist throttle valve (402) is arranged below each carbon dioxide throttle valve (403), and the mist throttle valve (402) is connected with the mist delivery branch pipe.
4. The carbon dioxide incubator of claim 1, wherein a thermal insulation layer is attached to each face within each of the inner cavities (302);
and a silicon rubber sealing strip is arranged between the inner glass door (301) and the inner cavity (302) and between the outer box door (103) and the independent cavity (104).
5. The carbon dioxide incubator of claim 1, wherein copper tubes (303) are also secured to the inside of each outer door (103).
6. A carbon dioxide incubator as claimed in claim 1, wherein an ultraviolet lamp is provided on the top wall of each of the inner cavities (302).
7. The carbon dioxide incubator of claim 1, wherein two side walls of the inner chamber (302) are provided with laminate support plates (308) with vertical bottom surfaces, and the laminate support plates (308) are provided with grooves in horizontal directions.
8. The carbon dioxide incubator of claim 1, wherein a sensor protection cover (305) is provided on top of each inner cavity (302), and a carbon dioxide concentration sensor, a humidity sensor and a temperature sensor are provided in the sensor protection cover (305).
9. The carbon dioxide incubator of claim 1, wherein the copper tube (305) is vented with a heating liquid or vapor.
CN201911019188.3A 2019-10-24 2019-10-24 Carbon dioxide incubator Active CN110669668B (en)

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CN110162123A (en) * 2019-04-30 2019-08-23 福建九圃生物科技有限公司 A kind of chamber of precise control of temperature humidity

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