CN205387577U - Novel carbon dioxide cell incubator - Google Patents

Novel carbon dioxide cell incubator Download PDF

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Publication number
CN205387577U
CN205387577U CN201620215793.3U CN201620215793U CN205387577U CN 205387577 U CN205387577 U CN 205387577U CN 201620215793 U CN201620215793 U CN 201620215793U CN 205387577 U CN205387577 U CN 205387577U
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China
Prior art keywords
mixing chamber
cell culture
carbon dioxide
conduit
grades
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Expired - Fee Related
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CN201620215793.3U
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Chinese (zh)
Inventor
李秀杰
李�杰
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Hubei Xinhua Zhongke Biotechnology Co Ltd
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Hubei Xinhua Zhongke Biotechnology Co Ltd
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Priority to CN201620215793.3U priority Critical patent/CN205387577U/en
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Abstract

The utility model belongs to the cell culture field, concretely relates to novel carbon dioxide cell incubator, comprising a main body, the cultivation room that the main part was set up by the horizontal direction in proper order side by side, the second grade mixing chamber is constituteed with elementary mixing chamber, it is separated by first vertical baffle with the second grade mixing chamber to cultivate the room, the lower part of first vertical baffle is provided with a plurality of through -holes no. 1 that room and second grade mixing chamber were cultivateed to the intercommunication, the second grade mixing chamber is separated by the vertical baffle of second with elementary mixing chamber, the upper portion of the vertical baffle of second is provided with a plurality of through -holes no. 2 of intercommunication second grade mixing chamber and elementary mixing chamber, cultivate the room and pass through the induction -pipe intercommunication with elementary mixing chamber, be provided with electronic air pump on the induction -pipe. Make to cultivate indoor gas through electronic air pump and be in flow state, be favorable to cultivateing indoor carbon dioxide's evenly distributed, set up elementary mixing chamber and second grade mixing chamber, be favorable to carrying out the preliminary treatment of temperature, humidity and carbon dioxide concentration to letting in the gas of cultivateing the room.

Description

A kind of Novel carbon dioxide cell culture incubator
Technical field
This utility model belongs to field of cell culture, is specifically related to a kind of Novel carbon dioxide cell culture incubator.
Background technology
CO2 gas incubator is widely used in the cultivation of cell, tissue culture and some peculiar microorganism, is common in cyto-dynamics research, the collection of mammalian cell secretions, various physics, the carcinogenic or toxicological effect of chemical factor, the research of antigen and production, cultivation hybridoma and produces the research fields such as antibody, in vitro fertilization (IVF), stem cell, organizational project, drug screening.
CO2 gas incubator is to form similar cell/tissue growing environment in vivo by simulation in incubator box body, such as stable temperature (37 DEG C), stable carbon dioxide level (5%), constant acid-base value (pH value: 7.2-7.4), higher relative saturation humidity (95%), cell/tissue is carried out a kind of device of In vitro culture.
Current CO2 gas incubator exists following not enough: the gas in incubator is substantially immobilising, then in incubator, carbon dioxide is likely to be formed more greatly the Concentraton gradient of in the vertical direction because of density, and in whole incubator, gas concentration lwevel is uneven;Heating, humidification and gas concentration lwevel are directly set in the culturing room of incubator and regulate device, there is a need to regular hour and space because of heat and mass, cause that the cell growth conditions being in culturing room near heating, humidification and gas concentration lwevel adjustment device has significantly different with its elsewhere.
Utility model content
In order to overcome the deficiencies in the prior art, this utility model provides a kind of Novel carbon dioxide cell culture incubator.
The technical scheme that this utility model solves above-mentioned technical problem is as follows: a kind of Novel carbon dioxide cell culture incubator, including main body, the culturing room that described main body is set up in parallel successively by horizontal direction, two grades of mixing chambers and primary mixing chamber composition, described culturing room is provided with the chamber door of rotatable folding, described culturing room and described two grades of mixing chambers are separated by the first vertical clapboard, the bottom of described first vertical clapboard is provided with the some through holes one connecting described culturing room and described two grades of mixing chambers, described two grades of mixing chambers are separated by the second vertical clapboard with described primary mixing chamber, the top of described second vertical clapboard is provided with the some through holes two connecting described two grades of mixing chambers with described primary mixing chamber, described culturing room is connected by snorkel with described primary mixing chamber, described snorkel is provided with electric air pump;
Described primary mixing chamber is also communicated with a CO2Conduit and the first pure air conduit, described two grades of mixing chambers are also communicated with the 2nd CO2Conduit and the second pure air conduit, a described CO2Conduit, the first pure air conduit, the 2nd CO2Conduit and the second pure air conduit are all each provided with electromagnetic valve, it is provided with first sensor module, primary heater and the first humidifier in described primary mixing chamber, it is provided with the second sensor assembly, secondary heater and the second humidifier in described two grades of mixing chambers, being additionally provided with microprocessor in described main body, described first sensor module, primary heater, the first humidifier, the second sensor assembly, secondary heater, the second humidifier and electromagnetic valve are all connected with described microprocessor signals.
Real time temperature, humidity and the dense carbon dioxide degrees of data that first sensor module and the second sensor assembly detect and transmit can be compared with preset data and judge by described microprocessor, send to described primary heater, secondary heater, the first humidifier, the second humidifier and suitable be turned on and off order, to adjust temperature and humidity;Send to specific electromagnetic valve simultaneously and be turned on and off instruction, to adjust the concentration of carbon dioxide.
On the basis of technique scheme, this utility model can also do following improvement.
Further, described first sensor module and the second sensor assembly are all integrated with temperature sensor, humidity sensor and gas concentration lwevel sensor.
Further, described culturing room is separated into some cell culture units by upper and lower spaced some horizontal baffles, offering the passage connecting its upper and lower two cell culture units on described horizontal baffle, the through hole one of described first vertical clapboard bottom connects with the cell culture unit of culturing room's bottom.
Further, the surface of described horizontal baffle is provided with polytetrafluorethylecoatings coatings.Polytetrafluorethylecoatings coatings, smooth not pickup, easy to clean.
Further, it is provided with uviol lamp in each described cell culture unit.Described uviol lamp can to cell culture unit disinfection.
Further, described uviol lamp is arranged on described first vertical clapboard.
Further, described second sensor assembly, secondary heater and the second humidifier are arranged on described second vertical clapboard.
Further, described main body is additionally provided with manual control panel, described manual control panel and described microprocessor and the electrical connection of described electric air pump.Preset data by microprocessor described in described manual control panel adjustable.
Further, the top of described culturing room and the top of described primary mixing chamber are connected by described snorkel.
Further, a described CO2Conduit and the first pure air conduit stretch in described primary mixing chamber and its outlet is near described through hole two, described 2nd CO2Conduit and the second pure air conduit stretch in described primary mixing chamber and its outlet is near the bottom of described primary mixing chamber.
The beneficial effects of the utility model are: culturing room, two grades of mixing chambers and primary mixing chamber are set up in parallel, in position it is sequentially communicated simultaneously, make the gas in culturing room be in flow regime by electric air pump, be conducive to cultivating being uniformly distributed of indoor carbon dioxide;Gas in culturing room carried out the pretreatment of temperature, humidity and gas concentration lwevel in advance before passing in it in primary mixing chamber and two grades of mixing chambers, there are time enough and space make gas mix homogeneously and guarantee that the data such as temperature, humidity and gas concentration lwevel meet the needs that cell is cultivated, make all cells in culturing room be in homogeneous good cultivation atmosphere;Primary and two grades of mixing chambers are used in combination, first coarse adjustment fine tuning again, are conducive to each item data of mixing gas to reach pre-provisioning request.
Accompanying drawing explanation
The structural representation of a kind of Novel carbon dioxide cell culture incubator that Fig. 1 provides for this utility model;
Fig. 2 is the structural representation of the first vertical clapboard between culturing room and two grades of mixing chambers;
Fig. 3 is the structural representation of the second vertical clapboard between primary mixing chamber and two grades of mixing chambers.
In accompanying drawing, the list of parts representated by each label is as follows:
1. main body;2. two grades of mixing chambers;3. primary mixing chamber;4. chamber door;5. horizontal baffle;6. passage;7. snorkel;8. electric air pump;9. first sensor module;10. the second sensor assembly;11. primary heater;12. secondary heater;13. the first humidifier;14. the second humidifier;15. the second pure air conduit;16. the 2nd CO2Conduit;17. the first pure air conduit;18. a CO2Conduit;19. electromagnetic valve;20. microprocessor;21. through hole one;22. uviol lamp;23. through hole two.
Detailed description of the invention
Below in conjunction with accompanying drawing, principle of the present utility model and feature being described, example is served only for explaining this utility model, is not intended to limit scope of the present utility model.
nullAs shown in Figure 1 to Figure 3,This utility model provides a kind of Novel carbon dioxide cell culture incubator,Including main body 1,The culturing room that described main body 1 is set up in parallel successively by horizontal direction、Two grades of mixing chambers 2 and primary mixing chamber 3 form,Described culturing room is provided with the chamber door 4 of rotatable folding,Described culturing room and described two grades of mixing chambers 2 are separated by the first vertical clapboard,The bottom of described first vertical clapboard is provided with the some through holes 1 connecting described culturing room and described two grades of mixing chambers 2,Described two grades of mixing chambers 2 are separated by the second vertical clapboard with described primary mixing chamber 3,The top of described second vertical clapboard is provided with some through holes 2 23 of the described two grades of mixing chambers of connection 2 and described primary mixing chamber 3,Described culturing room is connected by snorkel 7 with described primary mixing chamber (3),Described snorkel 7 is provided with electric air pump 8;
Described primary mixing chamber 3 is also communicated with a CO2Conduit 18 and the first pure air conduit 17, described two grades of mixing chambers 2 are also communicated with the 2nd CO2Conduit 16 and the second pure air conduit 15, a described CO2Conduit the 18, first pure air conduit the 17, the 2nd CO2Conduit 16 and the second pure air conduit 15 are all each provided with electromagnetic valve 19, it is provided with first sensor module 9, primary heater 11 and the first humidifier 13 in described primary mixing chamber 3, the second sensor assembly 10, secondary heater 12 and the second humidifier 14 it is provided with in described two grades of mixing chambers 2, being additionally provided with microprocessor 20 in described main body 1, described first sensor module 9, primary heater the 11, first humidifier the 13, second sensor assembly 10, secondary heater the 12, second humidifier 14 and electromagnetic valve 19 are all connected with described microprocessor 20 signal.
On the basis of technique scheme, this utility model can also do following improvement.
Further, described first sensor module 9 and the second sensor assembly 10 are all integrated with temperature sensor, humidity sensor and gas concentration lwevel sensor.
Further, described culturing room is separated into some cell culture units by upper and lower spaced some horizontal baffles 5, offering the passage 6 connecting its upper and lower two cell culture units on described horizontal baffle 5, the through hole 1 of described first vertical clapboard bottom connects with the cell culture unit of culturing room's bottom.
Further, the surface of described horizontal baffle 5 is provided with polytetrafluorethylecoatings coatings.
Further, it is provided with uviol lamp 22 in each described cell culture unit.
Further, described uviol lamp 22 is arranged on described first vertical clapboard.
Further, described second sensor assembly 10, secondary heater 12 and the second humidifier 14 are arranged on described second vertical clapboard.
Further, described main body 1 being additionally provided with manual control panel, described manual control panel is electrically connected with described microprocessor 20 and described electric air pump 8.
Further, the top of described culturing room and the top of described primary mixing chamber 3 are connected by described snorkel 7.
Further, a described CO2Conduit 18 and the first pure air conduit 17 stretch in described two grades of mixing chambers 2 and its outlet is near described through hole 2 23, described 2nd CO2Conduit 16 and the second pure air conduit 15 stretch in described primary mixing chamber 3 and its outlet is near the bottom of described primary mixing chamber 3.
The foregoing is only preferred embodiment of the present utility model, not in order to limit this utility model, all within spirit of the present utility model and principle, any amendment of making, equivalent replacement, improvement etc., should be included within protection domain of the present utility model.

Claims (10)

  1. null1. a Novel carbon dioxide cell culture incubator,It is characterized in that,Including main body (1),The culturing room that described main body (1) is set up in parallel successively by horizontal direction、Two grades of mixing chambers (2) and primary mixing chamber (3) composition,Described culturing room is provided with the chamber door (4) of rotatable folding,Described culturing room and described two grades of mixing chambers (2) are separated by the first vertical clapboard,The bottom of described first vertical clapboard is provided with the some through holes one (21) connecting described culturing room and described two grades of mixing chambers (2),Described two grades of mixing chambers (2) are separated by the second vertical clapboard with described primary mixing chamber (3),The top of described second vertical clapboard is provided with the some through holes two (23) connecting described two grades of mixing chambers (2) with described primary mixing chamber (3),Described culturing room is connected by snorkel (7) with described primary mixing chamber (3),Described snorkel (7) is provided with electric air pump (8);
    Described primary mixing chamber (3) is also communicated with a CO2Conduit (18) and the first pure air conduit (17), described two grades of mixing chambers (2) are also communicated with the 2nd CO2Conduit (16) and the second pure air conduit (15), a described CO2Conduit (18), the first pure air conduit (17), the 2nd CO2Conduit (16) and the second pure air conduit (15) are all each provided with electromagnetic valve (19), it is provided with first sensor module (9) in described primary mixing chamber (3), primary heater (11) and the first humidifier (13), it is provided with the second sensor assembly (10) in described two grades of mixing chambers (2), secondary heater (12) and the second humidifier (14), described main body (1) is additionally provided with microprocessor (20), described first sensor module (9), primary heater (11), first humidifier (13), second sensor assembly (10), secondary heater (12), second humidifier (14) and electromagnetic valve (19) are all connected with described microprocessor (20) signal.
  2. 2. a kind of Novel carbon dioxide cell culture incubator according to claim 1, it is characterized in that, described first sensor module (9) and the second sensor assembly (10) are all integrated with temperature sensor, humidity sensor and gas concentration lwevel sensor.
  3. 3. a kind of Novel carbon dioxide cell culture incubator according to claim 1, it is characterized in that, described culturing room is separated into some cell culture units by upper and lower spaced some horizontal baffles (5), offering the passage (6) connecting its upper and lower two cell culture units on described horizontal baffle (5), the through hole one (21) of described first vertical clapboard bottom connects with the cell culture unit of culturing room's bottom.
  4. 4. a kind of Novel carbon dioxide cell culture incubator according to claim 3, it is characterised in that the surface of described horizontal baffle (5) is provided with polytetrafluorethylecoatings coatings.
  5. 5. a kind of Novel carbon dioxide cell culture incubator according to claim 3, it is characterised in that be provided with uviol lamp (22) in each described cell culture unit.
  6. 6. a kind of Novel carbon dioxide cell culture incubator according to claim 5, it is characterised in that described uviol lamp (22) is arranged on described first vertical clapboard.
  7. 7. a kind of Novel carbon dioxide cell culture incubator according to claim 1, it is characterized in that, described second sensor assembly (10), secondary heater (12) and the second humidifier (14) are arranged on described second vertical clapboard.
  8. 8. a kind of Novel carbon dioxide cell culture incubator according to claim 1, it is characterized in that, described main body (1) is additionally provided with manual control panel, described manual control panel and described microprocessor (20) and described electric air pump (8) electrical connection.
  9. 9. a kind of Novel carbon dioxide cell culture incubator according to any one of claim 1 to 8, it is characterised in that the top of described culturing room and the top of described primary mixing chamber (3) are connected by described snorkel (7).
  10. 10. a kind of Novel carbon dioxide cell culture incubator according to any one of claim 1 to 8, it is characterised in that described 2nd CO2Conduit (16) and the second pure air conduit (15) stretch in described two grades of mixing chambers (2) and its outlet is near described through hole two (23), a described CO2Conduit (17) and the first pure air conduit (18) stretch in described primary mixing chamber (3) and its outlet is near the bottom of described primary mixing chamber (3).
CN201620215793.3U 2016-03-21 2016-03-21 Novel carbon dioxide cell incubator Expired - Fee Related CN205387577U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201620215793.3U CN205387577U (en) 2016-03-21 2016-03-21 Novel carbon dioxide cell incubator

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201620215793.3U CN205387577U (en) 2016-03-21 2016-03-21 Novel carbon dioxide cell incubator

Publications (1)

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CN205387577U true CN205387577U (en) 2016-07-20

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Country Status (1)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107164228A (en) * 2017-05-12 2017-09-15 杨健 CO2gas incubator
CN111321072A (en) * 2020-03-05 2020-06-23 通用生物系统(安徽)有限公司 Cell culture box for preparing monoclonal antibody

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107164228A (en) * 2017-05-12 2017-09-15 杨健 CO2gas incubator
CN111321072A (en) * 2020-03-05 2020-06-23 通用生物系统(安徽)有限公司 Cell culture box for preparing monoclonal antibody

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160720

Termination date: 20170321

CF01 Termination of patent right due to non-payment of annual fee