CN109439544A - A kind of carbon dioxide cell incubator - Google Patents

A kind of carbon dioxide cell incubator Download PDF

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Publication number
CN109439544A
CN109439544A CN201811318795.5A CN201811318795A CN109439544A CN 109439544 A CN109439544 A CN 109439544A CN 201811318795 A CN201811318795 A CN 201811318795A CN 109439544 A CN109439544 A CN 109439544A
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China
Prior art keywords
shell
carbon dioxide
cell incubator
dioxide cell
plc controller
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201811318795.5A
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Chinese (zh)
Inventor
李博文
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Zhengzhou Welt Biotechnology Co Ltd
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Zhengzhou Welt Biotechnology Co Ltd
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Priority to CN201811318795.5A priority Critical patent/CN109439544A/en
Publication of CN109439544A publication Critical patent/CN109439544A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Computer Hardware Design (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a kind of carbon dioxide cell incubators, including shell, the bottom of the shell is equipped with carbon dioxide generating device and humidifier, the middle part of shell is equipped with several supporting plates for culture dish holding in parallel, temperature sensor is installed at the top of the shell, humidity sensor, gas concentration lwevel sensor and ultraviolet lamp, PLC controller is installed above the shell, display screen and control key are provided in PLC controller, the shell is inner hollow framework, the inside of shell is filled with heat-preservation cotton, and enclosure interior has been fitted around electric heating wire, compared with common cell incubator, the present invention passes through carbon dioxide generating device, the effect of humidifier and electric heating wire, it can be realized to the gas concentration lwevel inside incubator, temperature and humidity is accurately controlled, it ensure that cell Normal growth;Meanwhile present invention automation, intelligence degree height, improve the working efficiency of staff.

Description

A kind of carbon dioxide cell incubator
Technical field
The present invention relates to field of biotechnology, and in particular to a kind of carbon dioxide cell incubator.
Background technique
In recent years, with the development of science and technology the development of biotechnology is also more and more mature.In field of biotechnology, carefully Born of the same parents' culture technique is a kind of important method for Celluar and Molecular Biology research, plays irreplaceable work With.During cell culture, carbon dioxide cell incubator is a kind of common instrument for cell and tissue structrue, i.e., logical It crosses and intends forming a similar cell, the growing environment of tissue in vivo in culture tank shell internal model, it is ensured that in incubator Humidity, temperature and carbon dioxide meet the growth demand of cell, to cultivate cell, tissue, and then obtain corresponding Cell, tissue products.
In the prior art, most of carbon dioxide cell incubator, the control for humidity, temperature and carbon dioxide It is very inconvenient, it needs manually regularly checked, adjusted, it is intelligent not high, seriously affect the growth ring of incubator inner cell Border, it is very unfavorable to the growth of cell.
Summary of the invention
The purpose of the present invention is to provide a kind of carbon dioxide cell incubators, mentioned above in the background art to solve Problem.
To achieve the above object, the invention provides the following technical scheme:
A kind of carbon dioxide cell incubator, including shell, the bottom of the shell are equipped with carbon dioxide generating device and add Wet device, the middle part of shell are equipped with several supporting plates in parallel, are placed with several culture dishes, institute on each supporting plate It states and several ultraviolet lamps is installed at the top of shell, and be also equipped with temperature sensor, humidity sensor and two at the top of shell Carbonoxide concentration sensor, the shell top are equipped with PLC controller, are provided with display screen and control key in PLC controller, The shell is inner hollow framework, and the inside of shell is filled with heat-preservation cotton, and enclosure interior has been fitted around electric heating wire.
As a further solution of the present invention: the side wall of the shell is provided with ventilation fan and air outlet, and air outlet is located at The lower section of ventilation fan.
As further scheme of the invention: the ventilation fan is unidirectional ventilation fan, and air outlet is unidirectional air outlet.
As further scheme of the invention: the PLC controller is filled with carbon dioxide generating device, humidification respectively Set, ventilation fan, ultraviolet lamp, temperature sensor, humidity sensor, gas concentration lwevel sensor and electric heating wire are electrical connected, And control its work.
As further scheme of the invention: the left and right sides of each supporting plate is symmetrically connected with several cunnings Wheel, pulley are slidably arranged in track, and the track is fixedly mounted on the side wall of shell.
As further scheme of the invention: being provided with several through-holes on each supporting plate.
As the present invention further scheme: the front of the shell is hinged with chamber door, be provided on chamber door handle and Visor.
As further scheme of the invention: the visor is made of transparent tempered glass.
Compared with common cell incubator, the beneficial effects of the present invention are: the present invention by carbon dioxide generating device, The effect of humidifier and electric heating wire can be realized and control to the gas concentration lwevel inside incubator, temperature and humidity System, ensure that the normal growth of cell, improves culture efficiency;Meanwhile the present invention can be realized by PLC controller to dioxy Change concentration of carbon, the automation of temperature and humidity, intelligentized control method and improves staff's without manually periodically being operated Working efficiency.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of carbon dioxide cell incubator.
Fig. 2 is a kind of cross-sectional view of shell in carbon dioxide cell incubator.
Fig. 3 is a kind of front view of carbon dioxide cell incubator.
In figure: 1- shell, 2- carbon dioxide generating device, 3- humidifier, 4- air outlet, 5- track, 6- pulley, 7- support Plate, 8- through-hole, 9- culture dish, 10- ventilation fan, 11- ultraviolet lamp, 12- temperature sensor, 13- humidity sensor, 14- titanium dioxide Concentration of carbon sensor, 15-PLC controller, 16- display screen, 17- control key, 18- heat-preservation cotton, 19- electric heating wire, 20- chamber door, 21- handle, 22- visor.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other Embodiment shall fall within the protection scope of the present invention.
Embodiment 1
Please refer to Fig. 1~2, in the embodiment of the present invention, a kind of carbon dioxide cell incubator, including shell 1, the shell 1 Bottom is equipped with carbon dioxide generating device 2 and humidifier 3, and the middle part of shell 1 is equipped with several supporting plates 7 in parallel, each Several are placed on the supporting plate 7 for cultivating the culture dish 9 of cell, the top of the shell 1 is equipped with several Ultraviolet lamp 11, and the top of shell 1 is also equipped with temperature sensor 12, humidity sensor 13 and gas concentration lwevel sensor 14, PLC controller 15 is installed above the shell 1, display screen 16 and control key 17 are provided in PLC controller 15, it is described Shell 1 is inner hollow framework, and the inside of shell 1 is filled with heat-preservation cotton 18, and 1 inner loop of shell is around being equipped with electric heating wire 19。
Further, the side wall of the shell 1 is provided with ventilation fan 10 and air outlet 4, and air outlet 4 is located at ventilation fan 10 Lower section.
Further, the ventilation fan 10 is unidirectional ventilation fan, and air outlet 4 is unidirectional air outlet, here, ventilation fan 10 Place's gas can only enter, and 4 gas of air outlet can only go out cannot be into.
Further, the PLC controller 15 respectively with carbon dioxide generating device 2, humidifier 3, ventilation fan 10, Ultraviolet lamp 11, temperature sensor 12, humidity sensor 13, gas concentration lwevel sensor 14 and electric heating wire 19 are electrical connected, And control its work.
The working principle of the present embodiment is: in the use of the present invention, connecting external power supply (not shown), staff Can be according to the characteristic for the cell cultivated, carbon dioxide concentration value required for setting is cultivated in PLC controller 15 in advance, At this moment temperature value and humidity value open carbon dioxide generating device 2 by PLC controller 15, when carbon dioxide is dense in culturing room 3 When angle value reaches preset value, gas concentration lwevel sensor 14 transmits a signal to PLC controller 15, PLC controller 15 Carbon-dioxide generator closing is controlled again, to realize accurately controlling to the density of carbon dioxide gas inside incubator. Meanwhile PLC controller 15 can also control electric heating according to the signal value of the transmission of temperature sensor 12 and humidity sensor 13 The unlatching of silk 19 and humidifier 3 is either closed, and guarantees that the temperature and humidity inside incubator is at a suitable cell In the range of growth.In addition, if gas concentration lwevel is excessively high, PLC controller 15 can be controlled and be changed when in cell cultivation process 10 work of gas fan, extraneous gas is delivered to inside incubator, the gas concentration lwevel inside incubator is reduced, and in incubator The ultraviolet lamp 11 in portion can play the role of sterilizing, guarantee that the cell in incubator is not contaminated.
It is compared with common cell incubator, the present invention passes through carbon dioxide generating device 2, humidifier 3 and electric heating The effect of silk 19, can be realized and control the gas concentration lwevel inside incubator, temperature and humidity, ensure that cell Normal growth improves culture efficiency;Meanwhile the present invention can be realized automation, intelligence by PLC controller 15, be not necessarily to It is manually periodically operated, improves the working efficiency of staff.
Embodiment 2
Referring to Fig. 1, the present embodiment mainly upgrades supporting plate 7, i.e., the left and right sides of each supporting plate 7 symmetrically connects Several pulleys 6 are connected to, pulley 6 is slidably arranged in track 5, and the track 5 is fixedly mounted on the side wall of shell 1.
Further, several through-holes 8 are provided on each supporting plate 7.
Here, when placing and taking out culture dish 9, pass through the effect of pulley 6 and track 5, it is only necessary to be pulled outwardly supporting plate 7 Out, facilitate the operation of staff.
Embodiment 3
Please refer to Fig. 1~3, in the present embodiment, the front of the shell 1 is hinged with chamber door 20, and handle 21 is provided on chamber door 20 With visor 22.
Further, the visor 22 is made of transparent tempered glass.
It should be strongly noted that in the technical program, carbon dioxide generating device 2, humidifier 3 and electric heating wire 19 Be the application of the prior art, and supporting plate 7, the specific installation of ventilation fan 10 and PLC controller 15, application, structure be the application Innovative point compared with common cell incubator, the beneficial effect is that: the present invention passes through the effect of pulley 6 and track 5, side Just the operation of staff, and the growing environment of incubator inner cell can accurately control, conducive to the life of cell It is long;Meanwhile automation, intelligence can be realized by PLC controller 15, without manually periodically being operated, improve work The working efficiency of personnel.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims Variation is included within the present invention.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art The other embodiments being understood that.

Claims (8)

1. a kind of carbon dioxide cell incubator, shell (1), which is characterized in that the bottom of the shell (1) is equipped with dioxy Change carbon generating device (2) and humidifier (3), the middle part of shell (1) and several supporting plates (7) be installed in parallel, it is each described in It is placed with several culture dishes (9) on supporting plate (7), several ultraviolet lamps (11), and shell are installed at the top of the shell (1) Temperature sensor (12), humidity sensor (13) and gas concentration lwevel sensor (14) are also equipped at the top of body (1), it is described It is equipped with PLC controller (15) above shell (1), display screen (16) and control key (17), institute is provided in PLC controller (15) Stating shell (1) is inner hollow framework, and the inside of shell (1) is filled with heat-preservation cotton (18), and shell (1) inner loop is around being equipped with Electric heating wire (19).
2. a kind of carbon dioxide cell incubator according to claim 1, which is characterized in that the side wall of the shell (1) It is provided with ventilation fan (10) and air outlet (4), air outlet (4) is located at the lower section of ventilation fan (10).
3. a kind of carbon dioxide cell incubator according to claim 2, which is characterized in that the ventilation fan (10) is single To ventilation fan, air outlet (4) is unidirectional air outlet.
4. a kind of carbon dioxide cell incubator according to claim 1, which is characterized in that the PLC controller (15) Respectively with carbon dioxide generating device (2), humidifier (3), ventilation fan (10), ultraviolet lamp (11), temperature sensor (12), wet Degree sensor (13), gas concentration lwevel sensor (14) and electric heating wire (19) are electrical connected, and control its work.
5. a kind of carbon dioxide cell incubator according to claim 1, which is characterized in that each supporting plate (7) The left and right sides is symmetrically connected with several pulleys (6), and pulley (6) is slidably arranged in track (5), and the track (5) is fixed It is mounted on the side wall of shell (1).
6. a kind of carbon dioxide cell incubator according to claim 1 or 5, which is characterized in that each supporting plate (7) several through-holes (8) are provided on.
7. a kind of carbon dioxide cell incubator according to claim 1, which is characterized in that the front of the shell (1) It is hinged with chamber door (20), handle (21) and visor (22) is provided on chamber door (20).
8. a kind of carbon dioxide cell incubator according to claim 7, which is characterized in that the visor (22) is using saturating Bright tempered glass is made.
CN201811318795.5A 2018-11-07 2018-11-07 A kind of carbon dioxide cell incubator Withdrawn CN109439544A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811318795.5A CN109439544A (en) 2018-11-07 2018-11-07 A kind of carbon dioxide cell incubator

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811318795.5A CN109439544A (en) 2018-11-07 2018-11-07 A kind of carbon dioxide cell incubator

Publications (1)

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CN109439544A true CN109439544A (en) 2019-03-08

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109943484A (en) * 2019-04-01 2019-06-28 上海东富龙医疗装备有限公司 A kind of RTP carbon dioxide incubator
CN110760442A (en) * 2019-10-09 2020-02-07 冯建兵 Carbon dioxide cell culture case
CN111321072A (en) * 2020-03-05 2020-06-23 通用生物系统(安徽)有限公司 Cell culture box for preparing monoclonal antibody
CN114107052A (en) * 2021-12-03 2022-03-01 深圳市新一仑生物科技有限公司 Portable stem cell amplification culture equipment capable of realizing online dynamic real-time monitoring

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109943484A (en) * 2019-04-01 2019-06-28 上海东富龙医疗装备有限公司 A kind of RTP carbon dioxide incubator
CN110760442A (en) * 2019-10-09 2020-02-07 冯建兵 Carbon dioxide cell culture case
CN111321072A (en) * 2020-03-05 2020-06-23 通用生物系统(安徽)有限公司 Cell culture box for preparing monoclonal antibody
CN114107052A (en) * 2021-12-03 2022-03-01 深圳市新一仑生物科技有限公司 Portable stem cell amplification culture equipment capable of realizing online dynamic real-time monitoring
CN114107052B (en) * 2021-12-03 2022-05-20 深圳市新一仑生物科技有限公司 Portable stem cell amplification culture equipment capable of realizing online dynamic real-time monitoring

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Application publication date: 20190308

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